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Gene information
General information
TC004827 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2498134..2499357
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC004827
      iBeetle screen iB_00773

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC004827 RA
        MGKLIVLLS FTLAVLAQE ELAKPVYKY RYATEDVMN 
        TAASTIPIH GYHVVEGGY YPGAMGMGG MGLGYGGYG 
        GGIGMGGAG MMGAGKFIG GMGGAGMMG GAGLGGAGL 
        VAAPLLHHG YGGEFIDKN VYSGEKKNL NDEAFEKAS 
        GKKGEEFNA GKEGFTKGV AAVKNNKGE SAYFNDAKG 
        AKKMAEDGK AYYGGQHFD KEGKHGGQF NEKKGHKKG 
        HVIKGFKSS HHKDETGKT EEFYDEEND EGAKLNFNG 
        EAGSFGEKG ASQFKGGHE AGEFAEGAA KKAGHFDNE 
        HFVDNSNAG HGNYGEKKY GGSGSVYGV NNGIDQHSL 
        LGHQENSKI YKHFPHHVP FY
      CDS sequence
      • >TC004827 RA
        atgggcaag ttgatcgtc ctgcttagc ttcaccctt 
        gcagtcctt gcccaggag gagctcgcc aaacccgtc 
        tacaaatac cgctacgct acggaagat gtgatgaac 
        accgccgcc tccaccatc cccatccat ggctaccac 
        gtcgtggag ggtggctac taccctgga gccatgggc 
        atgggagga atgggcctt ggctatggc ggctacggg 
        ggtggaatt ggcatgggc ggagctggg atgatggga 
        gccggtaaa ttcattgga ggcatggga ggagctgga 
        atgatggga ggagcagga cttggggga gctggactc 
        gttgctgca cctctcctc caccacgga tacggcggt 
        gaattcatc gacaagaac gtctacagc ggcgaaaag 
        aagaacttg aacgatgaa gctttcgaa aaagcgagc 
        gggaagaaa ggcgaagag ttcaatgct gggaaagaa 
        ggtttcacg aaaggagtt gccgcagtg aagaacaac 
        aagggcgaa tcggcctat ttcaacgat gccaaaggt 
        gccaagaaa atggctgaa gacggtaaa gcttactac 
        ggaggtcaa cacttcgac aaagaaggg aaacacggt 
        ggtcaattc aacgagaag aaagggcac aagaaggga 
        catgtcatc aaagggttc aaatcctcg caccataag 
        gacgaaacc ggcaaaacc gaagaattc tacgatgag 
        gagaatgac gaaggtgcg aaacttaac ttcaacgga 
        gaagctgga agctttggc gagaaaggc gcttcgcaa 
        ttcaaggga ggtcacgaa gctggggaa tttgccgaa 
        ggagctgcc aagaaagca ggacacttc gataacgaa 
        cactttgtt gataacagc aatgctgga catggaaac 
        tacggggag aagaaatat ggagggagc gggtctgtc 
        tatggtgtt aataatggt attgaccaa catagtttg 
        ttgggacac caagaaaat agcaagatc tacaaacat 
        ttcccacac catgtgccg ttctactga
      mRNA sequence
      • >TC004827 RA
        ccaagcatc gttcagtca ttaaccatc atgggcaag 
        ttgatcgtc ctgcttagc ttcaccctt gcagtcctt 
        gcccaggag gagctcgcc aaacccgtc tacaaatac 
        cgctacgct acggaagat gtgatgaac accgccgcc 
        tccaccatc cccatccat ggctaccac gtcgtggag 
        ggtggctac taccctgga gccatgggc atgggagga 
        atgggcctt ggctatggc ggctacggg ggtggaatt 
        ggcatgggc ggagctggg atgatggga gccggtaaa 
        ttcattgga ggcatggga ggagctgga atgatggga 
        ggagcagga cttggggga gctggactc gttgctgca 
        cctctcctc caccacgga tacggcggt gaattcatc 
        gacaagaac gtctacagc ggcgaaaag aagaacttg 
        aacgatgaa gctttcgaa aaagcgagc gggaagaaa 
        ggcgaagag ttcaatgct gggaaagaa ggtttcacg 
        aaaggagtt gccgcagtg aagaacaac aagggcgaa 
        tcggcctat ttcaacgat gccaaaggt gccaagaaa 
        atggctgaa gacggtaaa gcttactac ggaggtcaa 
        cacttcgac aaagaaggg aaacacggt ggtcaattc 
        aacgagaag aaagggcac aagaaggga catgtcatc 
        aaagggttc aaatcctcg caccataag gacgaaacc 
        ggcaaaacc gaagaattc tacgatgag gagaatgac 
        gaaggtgcg aaacttaac ttcaacgga gaagctgga 
        agctttggc gagaaaggc gcttcgcaa ttcaaggga 
        ggtcacgaa gctggggaa tttgccgaa ggagctgcc 
        aagaaagca ggacacttc gataacgaa cactttgtt 
        gataacagc aatgctgga catggaaac tacggggag 
        aagaaatat ggagggagc gggtctgtc tatggtgtt 
        aataatggt attgaccaa catagtttg ttgggacac 
        caagaaaat agcaagatc tacaaacat ttcccacac 
        catgtgccg ttctactga ttttagaaa ctgggttct 
        agatcgtaa atatttatt ttatgttac catagatta 
        tttttgtct tgtctcttt tataacgaa tgccatgac 
        ttggtccaa tgttctttt tatgatact tatgtatta 
        tttgtatat tgtatataa tatatactt gaagcaatg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY