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Gene information
General information
TC004823 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid6: 356891..358048
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004823
    iBeetle screen iB_00772
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004823 RA
      MEIEQITCV PKTLSPEDQ HRLEQQNSR LVSDFKASQ 
      LEKDARKHW DLFYKRNEA RFFKDRHWT TREFRELLD 
      NQTAGTKRV LLEIGCGVG NFIFPLIEE QLNFDIIAC 
      DLSSKAIEI VRSNKLYNE GYMRAFQVD ITTEDVLNQ 
      VDANSVDIA TLIFVLSAI HPDKFVTTL RVIHKVLKP 
      GGVLLFRDY GLYDMAQLR FKAGHKISD NFYMRQDGT 
      RSYYFSVEF FSNLCVEAG FEVVSNAYV HRRTVNKKE 
      NIDVPRIFI QAKIRKPP
    CDS sequence
    • >TC004823 RA
      atggaaatc gaacaaatc acatgtgtc cccaaaacg 
      ttatcacct gaagaccaa caccgttta gagcagcaa 
      aattcgcgc ttagtatca gattttaaa gcttcacaa 
      ttagagaaa gatgctcgg aagcactgg gatttgttt 
      tataaacgt aacgaggca cgttttttc aaagaccgt 
      cactggaca actcgcgaa ttcagagaa ttgttggac 
      aatcaaaca gccggcacg aaaagggtg cttttggaa 
      attggatgc ggtgttggc aactttatt ttccccttg 
      attgaggaa cagctaaat tttgatata attgcttgc 
      gatttatcc tcgaaagcg attgagatc gtgcgaagc 
      aataagctt tacaatgaa gggtatatg agggcgttt 
      caagtcgat ataacaaca gaagatgtt ctaaaccaa 
      gtggatgcg aatagtgtg gatatcgcg acgctgatc 
      tttgtgtta tcagccata catcccgac aaatttgtg 
      accactttg agagttatc cacaaagta ttgaagccg 
      ggaggagtt ttgctcttc agggattat ggtttatac 
      gatatggct cagttgagg tttaaagcg ggtcataaa 
      atatcagat aatttttac atgagacag gatggaacg 
      aggagttac tacttttca gtcgagttt ttctcaaat 
      ttgtgcgtt gaagcgggg tttgaagtt gtaagtaat 
      gcatatgtg catcgaaga acggtcaat aagaaagaa 
      aacatcgat gtgccaaga atttttatt caagcaaaa 
      attagaaaa ccaccttga
    mRNA sequence
    • >TC004823 RA
      acgattaaa taatatggc tgcgttgtg ttgagttac 
      gctgagaac aggtgcctt gtgagcacg tttaccatt 
      tcatgtttt tatttttgt cgccaatgt gatggaaat 
      cgaacaaat cacatgtgt ccccaaaac gttatcacc 
      tgaagacca acaccgttt agagcagca aaattcgcg 
      cttagtatc agattttaa agcttcaca attagagaa 
      agatgctcg gaagcactg ggatttgtt ttataaacg 
      taacgaggc acgtttttt caaagaccg tcactggac 
      aactcgcga attcagaga attgttgga caatcaaac 
      agccggcac gaaaagggt gcttttgga aattggatg 
      cggtgttgg caactttat tttcccctt gattgagga 
      acagctaaa ttttgatat aattgcttg cgatttatc 
      ctcgaaagc gattgagat cgtgcgaag caataagct 
      ttacaatga agggtatat gagggcgtt tcaagtcga 
      tataacaac agaagatgt tctaaacca agtggatgc 
      gaatagtgt ggatatcgc gacgctgat ctttgtgtt 
      atcagccat acatcccga caaatttgt gaccacttt 
      gagagttat ccacaaagt attgaagcc gggaggagt 
      tttgctctt cagggatta tggtttata cgatatggc 
      tcagttgag gtttaaagc gggtcataa aatatcaga 
      taattttta catgagaca ggatggaac gaggagtta 
      ctacttttc agtcgagtt tttctcaaa tttgtgcgt 
      tgaagcggg gtttgaagt tgtaagtaa tgcatatgt 
      gcatcgaag aacggtcaa taagaaaga aaacatcga 
      tgtgccaag aatttttat tcaagcaaa aattagaaa 
      accaccttg aggtaagtt ttttaagtg attctgcac 
      ttctaaatt aatagttac ctttgttta caggtattt 
      agtggcgaa aatatgatc agatttaag gttagtatt 
      aattagaaa tatccttac agattgtcg gattttttg 
      tgatgaatt