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Gene information
General information
TC004819 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2392569..2396657
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004819
    iBeetle screen iB_00771

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Adult morphology
    motility decreased -

    Fertility
    egg production: number no/almost no eggs -
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4

    mature egg not present - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004819 RA
      MYRRRLSQT FPTYPNLKI LLLENKNLS EQCYRITLM 
      LRQISAANN VKQRLELIL KNSKLFGVL KDKFSAVPR 
      SLKDQSSSV LRVWAHQFE CVMAHRLRR GQQMFCLYS 
      KLWEERALR EFLRQMRQR IGVKSRDFL VAALGVAAY 
      DWSSNRIPE SEIRRHSKE LEYVYKLKE KTLCNKCIK 
      NSRICQCQK PSDSIEKPK LASYDDWKL FIEKNDLVV 
      WRRLHSSGN YEYKVYGSY ADVSAEDFL NVQIDIDYR 
      RQWDNTAVV LNIVEADPD PHSHSDIIY WEMQWPTLF 
      ANRDYVFNR RYLVDYKAK TLFLVSKST KHPSCPKQQ 
      TKYRIEDYW SYMVIRPYT ELNKPGIEF SLTYFDNPG 
      VNIPASVTT WVAMKAMPD FLERLREAS RKYKVYCKE 
      SGVCSICNI LDSHENNRE PYQWDYFED DVTDNTIYE 
      AHHKIKAPE MDTKPSNPP PKSDGDVDG TTNTSANVQ 
      SENHSFWRF IKRLFK
    • >TC004819 RB
      MYRRRLSQT FPTYPNLKI LLLENKNLS EQCYRITLM 
      LRQISAANN VKQRLELIL KNSKLFGVL KDKFSAVPR 
      SLKDQSSSV LRVWAHQFE CVMAHRLRR GQQMFCLYS 
      KLWEERALR EFLRQMRQR IGVKSRDFL VAALGVAAY 
      DWSSNRIPE SEIRRHSKE LEYVYKLKE KTLCNKCIK 
      NSRICQCQK PSDSIEKPK LASYDDWKL FIEKNDLVV 
      WRRLHSSGN YEYKVYGSY ADVSAEDFL NVQIDIDYR 
      RQWDNTAVV LNIVEADPD PHSHSDIIY WEMQWPTLF 
      ANRDYVFNR RYLVDYKAK TLFLVSKST KHPSCPKQQ 
      TKYRIEDYW SYMVIRPYT ELNKPGIEF SLTYFDNPG 
      VNIPASVTT WVAMKAMPD FLERLREAS RKYKVYCKE 
      SGVCSICNI LDSHENNRE PYQWDYFED DVTDNTIYE 
      AHHKIKAPE MDTKPSNPP PKSDGDVDG TTNTSANVQ 
      SENHSFWRY LHPTYYFS
    CDS sequence
    • >TC004819 RA
      atgtaccgc cggagacta tcccaaaca ttcccgact 
      taccctaac ctcaaaatc ctcctttta gaaaacaag 
      aacttaagc gagcagtgt taccgaata actttaatg 
      cttcgccag atttcagcc gcaaacaat gtcaaacaa 
      cgactcgaa ctgatcctc aaaaacagc aaactcttc 
      ggagtcctc aaagacaag ttctcggct gtaccccgc 
      tcccttaag gaccagtct tcgagcgtg ttgagggta 
      tgggcacac cagtttgag tgtgtgatg gcccaccgt 
      ttacgacgg ggccagcag atgttttgt ctctactcg 
      aaattgtgg gaggaaagg gctttaagg gagtttctg 
      agacagatg aggcagaga attggggtg aaaagccgg 
      gattttctg gtggcggcg ctgggggtc gccgcctat 
      gactggagc tcgaatcgc atcccggag agcgaaatt 
      aggagacat tcaaaagag ttggagtat gtttacaag 
      ctcaaggaa aaaacctta tgtaacaag tgcataaaa 
      aattcacgg atctgccaa tgccaaaag cctagtgat 
      agcatcgag aagcctaaa ctggcgtcg tacgatgat 
      tggaaatta tttatcgaa aaaaatgac ttggtggtg 
      tggaggagg ttgcactcc tcaggaaac tacgaatat 
      aaggtttat gggtcttat gctgatgtc tctgctgaa 
      gattttctg aatgttcag attgatatt gattataga 
      agacagtgg gataacact gcggtggtt ttgaatatt 
      gtggaagct gacccagac ccacattcg catagcgac 
      attatctac tgggaaatg cagtggcca acgttattc 
      gccaacaga gactacgtg ttcaaccgg cgctatttg 
      gtcgactac aaagcgaaa accctcttt ctagtcagc 
      aaatcaacc aaacacccc agttgtcct aaacaacag 
      acaaaatac cgaatcgag gactactgg tcttacatg 
      gtcatcaga ccgtacacc gaactgaat aagcccggc 
      atcgaattt agtctaact tattttgat aatcccgga 
      gtgaatatt ccagcttct gtaacaaca tgggtggca 
      atgaaagcc atgcccgac tttctggaa cgcttgcga 
      gaagcctct cgaaaatat aaagtttac tgtaaagag 
      tcgggtgtg tgtagtatt tgcaatatc ctcgattcg 
      cacgaaaat aaccgggag ccgtaccaa tgggattac 
      ttcgaagac gatgttacg gataacacc atctacgaa 
      gctcatcac aaaatcaaa gcgcctgaa atggacaca 
      aaaccatca aatcctcca ccgaaatcc gatggggat 
      gttgatggc acaaccaac acatcggca aacgtgcaa 
      tcagaaaac catagcttc tggagattt attaaaaga 
      ttatttaaa tga
    • >TC004819 RB
      atgtaccgc cggagacta tcccaaaca ttcccgact 
      taccctaac ctcaaaatc ctcctttta gaaaacaag 
      aacttaagc gagcagtgt taccgaata actttaatg 
      cttcgccag atttcagcc gcaaacaat gtcaaacaa 
      cgactcgaa ctgatcctc aaaaacagc aaactcttc 
      ggagtcctc aaagacaag ttctcggct gtaccccgc 
      tcccttaag gaccagtct tcgagcgtg ttgagggta 
      tgggcacac cagtttgag tgtgtgatg gcccaccgt 
      ttacgacgg ggccagcag atgttttgt ctctactcg 
      aaattgtgg gaggaaagg gctttaagg gagtttctg 
      agacagatg aggcagaga attggggtg aaaagccgg 
      gattttctg gtggcggcg ctgggggtc gccgcctat 
      gactggagc tcgaatcgc atcccggag agcgaaatt 
      aggagacat tcaaaagag ttggagtat gtttacaag 
      ctcaaggaa aaaacctta tgtaacaag tgcataaaa 
      aattcacgg atctgccaa tgccaaaag cctagtgat 
      agcatcgag aagcctaaa ctggcgtcg tacgatgat 
      tggaaatta tttatcgaa aaaaatgac ttggtggtg 
      tggaggagg ttgcactcc tcaggaaac tacgaatat 
      aaggtttat gggtcttat gctgatgtc tctgctgaa 
      gattttctg aatgttcag attgatatt gattataga 
      agacagtgg gataacact gcggtggtt ttgaatatt 
      gtggaagct gacccagac ccacattcg catagcgac 
      attatctac tgggaaatg cagtggcca acgttattc 
      gccaacaga gactacgtg ttcaaccgg cgctatttg 
      gtcgactac aaagcgaaa accctcttt ctagtcagc 
      aaatcaacc aaacacccc agttgtcct aaacaacag 
      acaaaatac cgaatcgag gactactgg tcttacatg 
      gtcatcaga ccgtacacc gaactgaat aagcccggc 
      atcgaattt agtctaact tattttgat aatcccgga 
      gtgaatatt ccagcttct gtaacaaca tgggtggca 
      atgaaagcc atgcccgac tttctggaa cgcttgcga 
      gaagcctct cgaaaatat aaagtttac tgtaaagag 
      tcgggtgtg tgtagtatt tgcaatatc ctcgattcg 
      cacgaaaat aaccgggag ccgtaccaa tgggattac 
      ttcgaagac gatgttacg gataacacc atctacgaa 
      gctcatcac aaaatcaaa gcgcctgaa atggacaca 
      aaaccatca aatcctcca ccgaaatcc gatggggat 
      gttgatggc acaaccaac acatcggca aacgtgcaa 
      tcagaaaac catagcttc tggaggtat ttgcacccg 
      acttactat ttcagttag
    mRNA sequence
    • >TC004819 RA
      ttacgttca gtgaaaatg tcaaaattt tagtattcc 
      agtgccgaa tttttcgaa acaattcta ttcacctcg 
      tccaaaaac ccctcagaa tccaaaaat tactaaagt 
      ccctcaccg gccccagta tgtaccgcc ggagactat 
      cccaaacat tcccgactt accctaacc tcaaaatcc 
      tccttttag aaaacaaga acttaagcg agcagtgtt 
      accgaataa ctttaatgc ttcgccaga tttcagccg 
      caaacaatg tcaaacaac gactcgaac tgatcctca 
      aaaacagca aactcttcg gagtcctca aagacaagt 
      tctcggctg taccccgct cccttaagg accagtctt 
      cgagcgtgt tgagggtat gggcacacc agtttgagt 
      gtgtgatgg cccaccgtt tacgacggg gccagcaga 
      tgttttgtc tctactcga aattgtggg aggaaaggg 
      ctttaaggg agtttctga gacagatga ggcagagaa 
      ttggggtga aaagccggg attttctgg tggcggcgc 
      tgggggtcg ccgcctatg actggagct cgaatcgca 
      tcccggaga gcgaaatta ggagacatt caaaagagt 
      tggagtatg tttacaagc tcaaggaaa aaaccttat 
      gtaacaagt gcataaaaa attcacgga tctgccaat 
      gccaaaagc ctagtgata gcatcgaga agcctaaac 
      tggcgtcgt acgatgatt ggaaattat ttatcgaaa 
      aaaatgact tggtggtgt ggaggaggt tgcactcct 
      caggaaact acgaatata aggtttatg ggtcttatg 
      ctgatgtct ctgctgaag attttctga atgttcaga 
      ttgatattg attatagaa gacagtggg ataacactg 
      cggtggttt tgaatattg tggaagctg acccagacc 
      cacattcgc atagcgaca ttatctact gggaaatgc 
      agtggccaa cgttattcg ccaacagag actacgtgt 
      tcaaccggc gctatttgg tcgactaca aagcgaaaa 
      ccctctttc tagtcagca aatcaacca aacacccca 
      gttgtccta aacaacaga caaaatacc gaatcgagg 
      actactggt cttacatgg tcatcagac cgtacaccg 
      aactgaata agcccggca tcgaattta gtctaactt 
      attttgata atcccggag tgaatattc cagcttctg 
      taacaacat gggtggcaa tgaaagcca tgcccgact 
      ttctggaac gcttgcgag aagcctctc gaaaatata 
      aagtttact gtaaagagt cgggtgtgt gtagtattt 
      gcaatatcc tcgattcgc acgaaaata accgggagc 
      cgtaccaat gggattact tcgaagacg atgttacgg 
      ataacacca tctacgaag ctcatcaca aaatcaaag 
      cgcctgaaa tggacacaa aaccatcaa atcctccac 
      cgaaatccg atggggatg ttgatggca caaccaaca 
      catcggcaa acgtgcaat cagaaaacc atagcttct 
      ggagattta ttaaaagat tatttaaat gaaagctat 
      gttttaaag tacccgctt ctgaccatc ctgtagaag 
      ttgtcaaat tgacacgaa cccaagctg tgaagaact 
      caatgtcaa tctagccac tcatatttg catagttag 
      tacaacttg tgaaattgc aggtgggaa gaatgacca 
      agaaaccaa agaaaattg ttacgttat cacctgtta 
      aaatattta aggactctc tcgtctagt actaaataa 
      attattatg caactg
    • >TC004819 RB
      ttacgttca gtgaaaatg tcaaaattt tagtattcc 
      agtgccgaa tttttcgaa acaattcta ttcacctcg 
      tccaaaaac ccctcagaa tccaaaaat tactaaagt 
      ccctcaccg gccccagta tgtaccgcc ggagactat 
      cccaaacat tcccgactt accctaacc tcaaaatcc 
      tccttttag aaaacaaga acttaagcg agcagtgtt 
      accgaataa ctttaatgc ttcgccaga tttcagccg 
      caaacaatg tcaaacaac gactcgaac tgatcctca 
      aaaacagca aactcttcg gagtcctca aagacaagt 
      tctcggctg taccccgct cccttaagg accagtctt 
      cgagcgtgt tgagggtat gggcacacc agtttgagt 
      gtgtgatgg cccaccgtt tacgacggg gccagcaga 
      tgttttgtc tctactcga aattgtggg aggaaaggg 
      ctttaaggg agtttctga gacagatga ggcagagaa 
      ttggggtga aaagccggg attttctgg tggcggcgc 
      tgggggtcg ccgcctatg actggagct cgaatcgca 
      tcccggaga gcgaaatta ggagacatt caaaagagt 
      tggagtatg tttacaagc tcaaggaaa aaaccttat 
      gtaacaagt gcataaaaa attcacgga tctgccaat 
      gccaaaagc ctagtgata gcatcgaga agcctaaac 
      tggcgtcgt acgatgatt ggaaattat ttatcgaaa 
      aaaatgact tggtggtgt ggaggaggt tgcactcct 
      caggaaact acgaatata aggtttatg ggtcttatg 
      ctgatgtct ctgctgaag attttctga atgttcaga 
      ttgatattg attatagaa gacagtggg ataacactg 
      cggtggttt tgaatattg tggaagctg acccagacc 
      cacattcgc atagcgaca ttatctact gggaaatgc 
      agtggccaa cgttattcg ccaacagag actacgtgt 
      tcaaccggc gctatttgg tcgactaca aagcgaaaa 
      ccctctttc tagtcagca aatcaacca aacacccca 
      gttgtccta aacaacaga caaaatacc gaatcgagg 
      actactggt cttacatgg tcatcagac cgtacaccg 
      aactgaata agcccggca tcgaattta gtctaactt 
      attttgata atcccggag tgaatattc cagcttctg 
      taacaacat gggtggcaa tgaaagcca tgcccgact 
      ttctggaac gcttgcgag aagcctctc gaaaatata 
      aagtttact gtaaagagt cgggtgtgt gtagtattt 
      gcaatatcc tcgattcgc acgaaaata accgggagc 
      cgtaccaat gggattact tcgaagacg atgttacgg 
      ataacacca tctacgaag ctcatcaca aaatcaaag 
      cgcctgaaa tggacacaa aaccatcaa atcctccac 
      cgaaatccg atggggatg ttgatggca caaccaaca 
      catcggcaa acgtgcaat cagaaaacc atagcttct 
      ggaggtatt tgcacccga cttactatt tcagttaga 
      acacatgac accgtagtc attacgcat aagactgtc 
      tgtaccttt tgttaagtg ttaatccat tttgtactg 
      ttgtagttt gtatttaac gcaatgttt tgcagattt 
      attaaaaga ttatttaaa tgaaagcta tgttttaaa 
      gtacccgct tctgaccat cctgtagaa gttgtcaaa 
      ttgacacga acccaagct gtgaagaac tcaatgtca 
      atctagcca ctcatattt gcatagtta gtacaactt 
      gtgaaattg caggtggga agaatgacc aagaaacca 
      aagaaaatt gttacgtta tcacctgtt aaaatattt 
      aaggactct ctcgtctag tactaaata aattattat 
      gcaactg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY