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Gene information
General information
TC004817 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2298034..2301979
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC004817
      iBeetle screen iB_00770

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
       egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC004817 RA
        MDRCFKWPQ ESKSKNYEE DRELIFRLQ SAKRRLSPI 
VDDIVESAV DLIEKGDDH FVNECTLTP DQYQDVIWA 
SQMLRQIEY DRMNHNNYY LPNTNLIVN RVSVESVKK 
TRDKSAVVI DQATVVQLL QLYDQMGVI IQKMLNTNK 
ATKNVDVQK STDESRSDC RCDTTMPAS TTTTENNEV 
DSLTSEKSA LKPCIKSTC SAFKKSSSS KENGEDSER 
KSVSINDEV ETKVCDDPT IYIKIQCYT DCSETAEVD 
ATPKTSEVK CSKSCDSHY HIKLDGEPV TLKMNQTNH 
NEDPVFCIN ARSDDESDV KIVIKQCPE CRSTIIGSS 
TKIENPPES HESLTIHGT PQLDETNLE YSSDDSGKS 
TSPSECHCK EKKMCIDLA KLKELLGCS CDKPKMDVV 
IDSDSVEKS EVKVRELPP LMRRRVCSS MEFDVTTVK 
TRLREKEIE HKNAHIVLE LKQLGVDQI LRASKPAKC 
CRKAILNER QRTALPYCV SLRSLPLMS HDAEVIKQS 
VDMSVKKIK NAIDLTQAY KDKLAYYVS LGKDDFKQK 
QTPWVVPLY DMDLNGEED RFYVLKKEE AEEQVATLL 
GIVERLRRN DLSYNKNRC FVLRKPNMM RRITEWNES 
KSSVLSRRI NRKRIKRRM ELKFRNCKP NRRRRSWRS 
NIDRFGFPH QFNSLDVNF LQTTEISRF TSFAKCSNS 
GSDIPLNAF PCFNICNLD NKQQEAIVE KSKGSGDCS 
LSKMLDFSY LNKPEKSKF LTSVFRKKN SKRKKSLPA 
SNALTIFIP KSKGVKRKK KNVLEYYVG LVKGDLVWS 
NTLPLVPQN YDTYYNHAS ERKKVPVEE PIVATRVSR 
AESSREPAR SPSRSSRRS SGSATRRKR LTLPESNNE 
PQKTVTPSP EPQQVNAPE MAKVDETQE LKDESWFPH 
VVSETSLPN IQETFEKKE VEQAEAQPV EPRDDPHEE 
EFVEQQEEP EEQQEEPEE QQEEPEEQP PPPPQAVKS 
KCSTKQVCI KHDCKSGCK ASKACCSSA SQSSSPPPP 
PSSAPHSSS VPPSSARHS SSPPAPSAR HSSSPPPPS 
QKAGPGDIT IAIKKKRKG IVIEGRAPA SCNKEVCEI 
YKNKNLVPD KPEKQQKAK SSSDKPPKE QKFKVVAKD 
SVEIIINNN TISVTNNDK RKK
      CDS sequence
      • >TC004817 RA
        atggataga tgtttcaaa tggccacaa gaaagcaaa 
tctaaaaat tatgaagaa gacagagaa cttatcttt 
cgtttacaa tcagcaaaa cgcagacta tcacccatt 
gtggacgat attgttgaa tcagccgta gatctcatc 
gaaaaaggt gatgatcat tttgtgaac gagtgcaca 
ctcacacca gaccaatat caagatgta atttgggct 
tctcaaatg ttacgacag atagaatat gatagaatg 
aaccacaat aactattac ttaccgaac actaattta 
atagtgaac cgtgtaagt gtcgaaagc gtgaaaaaa 
actagagat aagtccgcc gtcgttata gatcaagct 
acagtagtg cagctattg cagctttac gaccaaatg 
ggtgttatt atacaaaaa atgttaaac accaataaa 
gcaaccaaa aatgttgac gttcagaaa tcgaccgat 
gaaagtcga tcagactgt aggtgtgat accacaatg 
ccggcctca acaaccacc acagagaac aacgaagtt 
gattcttta acttccgaa aaaagtgca ctcaaaccg 
tgcatcaaa tcgacatgc tcagcattc aagaagagt 
tcttctagc aaagagaac ggcgaggac tcggaaaga 
aaaagtgtt tcaatcaac gacgaagtg gagactaaa 
gtttgcgat gaccccacc atctatatc aaaatccag 
tgctacact gattgctct gaaacggca gaagtcgat 
gctacgccc aaaaccagc gaagttaaa tgctccaaa 
agttgcgac tctcattat cacattaaa ctggacgga 
gaaccagtc acattaaaa atgaatcag accaaccat 
aatgaagac cctgttttt tgcataaat gcgcgaagc 
gacgacgaa agtgatgtc aaaattgtg atcaaacaa 
tgtccagaa tgtagatca acaatcatt ggttcttcc 
acaaaaatc gaaaacccg ccagaatca cacgagtcg 
cttacaata cacggaaca ccacaactt gacgaaaca 
aatctggaa tattcatct gatgattct ggcaaatca 
acatcaccc tcggaatgt cattgcaag gagaagaaa 
atgtgtata gacctcgcc aagcttaaa gaactgctc 
ggttgttca tgcgataaa cccaaaatg gacgtggtt 
atagattca gacagtgtg gaaaaatca gaagtcaaa 
gttcgtgaa ctaccacct ttgatgaga cgtcgggtt 
tgttcctca atggaattc gatgtcacc accgttaaa 
acgaggctt cgagagaaa gaaatcgag cacaaaaac 
gcccacatc gtcttagag ttgaaacaa ctaggagtt 
gaccaaatt ttacgagct agcaaaccg gccaagtgc 
tgtcggaag gcaattctc aacgaaagg caaagaaca 
gcgcttcct tactgtgtc agcttgcga tcgttgcca 
ctgatgagc cacgatgcc gaagtcatc aaacaatca 
gttgatatg agcgtgaag aaaatcaaa aacgcgatc 
gatctgacc caagcttac aaagacaaa ctggcgtat 
tatgtcagc ttggggaaa gatgatttc aaacaaaag 
caaacaccg tgggttgtg ccactgtac gatatggat 
ttaaatggc gaagaagac cgcttctac gtgttgaag 
aaagaagaa gctgaggaa caagttgct acactactt 
ggaatcgtt gaaagactc cgtcggaac gatttaagt 
tacaacaaa aatcgatgt tttgttttg aggaaacca 
aacatgatg agaaggata acggagtgg aatgagtca 
aaatcgtca gttttgagc aggagaatt aaccggaaa 
cgcataaaa agacgtatg gaattaaaa ttcagaaat 
tgtaaacca aacagaagg aggcgaagt tggagaagt 
aatattgat aggtttggc tttccccac caatttaat 
tcacttgat gtgaatttt cttcaaacg actgaaatc 
agtagattt acttctttt gccaaatgt tccaatagt 
gggtcagat atccccttg aatgctttt ccctgtttt 
aatatttgt aatttggat aataagcag caagaagca 
atagttgaa aaaagtaaa ggaagcggt gattgctcc 
ctaagcaag atgttggat ttctcttat ctaaataaa 
cccgaaaaa agcaagttc ttaacaagc gtgtttcgt 
aagaaaaat tcaaaaaga aaaaaatcg ttgcctgct 
tctaacgct ctaaccatt tttataccc aaatcaaaa 
ggtgtcaaa cgcaagaaa aaaaatgtt ctagaatac 
tatgttggt ttagtaaag ggagatcta gtctggagt 
aacaccctt ccactagta ccccaaaac tacgacacc 
tattataac catgcaagt gaacgtaag aaagttcct 
gtggaagag cccatagtc gctactcgc gtttcgcgt 
gccgaatca agtcgagaa ccagcaagg tcaccttca 
agaagcagc agaagaagt tctggttct gctaccaga 
cgcaaacgt ctcacattg ccggaatca aataacgaa 
ccccaaaaa actgtcaca ccatctcca gaaccccag 
caagttaat gctccagaa atggctaaa gttgatgaa 
acacaagaa ctaaaagac gaatcttgg tttcctcac 
gttgttagc gagacttcc ttgcccaac atccaggaa 
acatttgaa aagaaagaa gttgaacag gcggaagcg 
caaccggtg gaaccaaga gatgatccg catgaagag 
gaatttgta gaacagcag gaagaacca gaagaacag 
caagaagaa cctgaagaa cagcaagaa gaacctgaa 
gaacaacca ccaccaccg ccacaagca gtaaaaagc 
aaatgttcg acaaaacaa gtgtgcatc aaacatgat 
tgtaagagt ggttgcaaa gcaagtaag gcttgttgt 
tcatcagct tctcaatct tcttctcct ccgcctcct 
ccttcatcg gctcctcat tcttcttcc gttcctcct 
tcatcggct cgtcattct tcttcccct cctgctcca 
tcggctcgg cattcttct tcccctcct cctccttcc 
caaaaagcc ggtccggga gatattaca attgctatt 
aaaaagaag cgaaaggga atagttatt gaaggaaga 
gctccagcg agttgtaac aaggaagtt tgtgaaatt 
tacaaaaac aaaaatttg gtaccggac aaacccgag 
aaacaacaa aaagctaaa agttcgtct gacaagccc 
ccgaaagag caaaagttc aaagttgta gccaaagat 
agtgtagaa ataataata aataacaac acgatttct 
gtgacaaat aacgacaaa aggaaaaag taa
      mRNA sequence
      • >TC004817 RA
        ccgtaattg tagaaatga gattatgaa acaagtaga 
tattaagta gattatcaa tagattcag aaaatttta 
gattttcgg tgattatat tcatttcct aggcgttgg 
tctaaaacg tcgtaaaat gtctagctg acacatgtt 
tgacatcat ctgattgat cccaatcga atgagtcca 
caataatca aaatttttg taacatgtc ttaattctt 
actgaaatt atctgtaat cacacccaa gaacagcag 
ttctcatca cagaatgga tagatgttt caaatggcc 
acaagaaag caaatctaa aaattatga agaagacag 
agaacttat ctttcgttt acaatcagc aaaacgcag 
actatcacc cattgtgga cgatattgt tgaatcagc 
cgtagatct catcgaaaa aggtgatga tcattttgt 
gaacgagtg cacactcac accagacca atatcaaga 
tgtaatttg ggcttctca aatgttacg acagataga 
atatgatag aatgaacca caataacta ttacttacc 
gaacactaa tttaatagt gaaccgtgt aagtgtcga 
aagcgtgaa aaaaactag agataagtc cgccgtcgt 
tatagatca agctacagt agtgcagct attgcagct 
ttacgacca aatgggtgt tattataca aaaaatgtt 
aaacaccaa taaagcaac caaaaatgt tgacgttca 
gaaatcgac cgatgaaag tcgatcaga ctgtaggtg 
tgataccac aatgccggc ctcaacaac caccacaga 
gaacaacga agttgattc tttaacttc cgaaaaaag 
tgcactcaa accgtgcat caaatcgac atgctcagc 
attcaagaa gagttcttc tagcaaaga gaacggcga 
ggactcgga aagaaaaag tgtttcaat caacgacga 
agtggagac taaagtttg cgatgaccc caccatcta 
tatcaaaat ccagtgcta cactgattg ctctgaaac 
ggcagaagt cgatgctac gcccaaaac cagcgaagt 
taaatgctc caaaagttg cgactctca ttatcacat 
taaactgga cggagaacc agtcacatt aaaaatgaa 
tcagaccaa ccataatga agaccctgt tttttgcat 
aaatgcgcg aagcgacga cgaaagtga tgtcaaaat 
tgtgatcaa acaatgtcc agaatgtag atcaacaat 
cattggttc ttccacaaa aatcgaaaa cccgccaga 
atcacacga gtcgcttac aatacacgg aacaccaca 
acttgacga aacaaatct ggaatattc atctgatga 
ttctggcaa atcaacatc accctcgga atgtcattg 
caaggagaa gaaaatgtg tatagacct cgccaagct 
taaagaact gctcggttg ttcatgcga taaacccaa 
aatggacgt ggttataga ttcagacag tgtggaaaa 
atcagaagt caaagttcg tgaactacc acctttgat 
gagacgtcg ggtttgttc ctcaatgga attcgatgt 
caccaccgt taaaacgag gcttcgaga gaaagaaat 
cgagcacaa aaacgccca catcgtctt agagttgaa 
acaactagg agttgacca aattttacg agctagcaa 
accggccaa gtgctgtcg gaaggcaat tctcaacga 
aaggcaaag aacagcgct tccttactg tgtcagctt 
gcgatcgtt gccactgat gagccacga tgccgaagt 
catcaaaca atcagttga tatgagcgt gaagaaaat 
caaaaacgc gatcgatct gacccaagc ttacaaaga 
caaactggc gtattatgt cagcttggg gaaagatga 
tttcaaaca aaagcaaac accgtgggt tgtgccact 
gtacgatat ggatttaaa tggcgaaga agaccgctt 
ctacgtgtt gaagaaaga agaagctga ggaacaagt 
tgctacact acttggaat cgttgaaag actccgtcg 
gaacgattt aagttacaa caaaaatcg atgttttgt 
tttgaggaa accaaacat gatgagaag gataacgga 
gtggaatga gtcaaaatc gtcagtttt gagcaggag 
aattaaccg gaaacgcat aaaaagacg tatggaatt 
aaaattcag aaattgtaa accaaacag aaggaggcg 
aagttggag aagtaatat tgataggtt tggctttcc 
ccaccaatt taattcact tgatgtgaa ttttcttca 
aacgactga aatcagtag atttacttc ttttgccaa 
atgttccaa tagtgggtc agatatccc cttgaatgc 
ttttccctg ttttaatat ttgtaattt ggataataa 
gcagcaaga agcaatagt tgaaaaaag taaaggaag 
cggtgattg ctccctaag caagatgtt ggatttctc 
ttatctaaa taaacccga aaaaagcaa gttcttaac 
aagcgtgtt tcgtaagaa aaattcaaa aagaaaaaa 
atcgttgcc tgcttctaa cgctctaac catttttat 
acccaaatc aaaaggtgt caaacgcaa gaaaaaaaa 
tgttctaga atactatgt tggtttagt aaagggaga 
tctagtctg gagtaacac ccttccact agtacccca 
aaactacga cacctatta taaccatgc aagtgaacg 
taagaaagt tcctgtgga agagcccat agtcgctac 
tcgcgtttc gcgtgccga atcaagtcg agaaccagc 
aaggtcacc ttcaagaag cagcagaag aagttctgg 
ttctgctac cagacgcaa acgtctcac attgccgga 
atcaaataa cgaacccca aaaaactgt cacaccatc 
tccagaacc ccagcaagt taatgctcc agaaatggc 
taaagttga tgaaacaca agaactaaa agacgaatc 
ttggtttcc tcacgttgt tagcgagac ttccttgcc 
caacatcca ggaaacatt tgaaaagaa agaagttga 
acaggcgga agcgcaacc ggtggaacc aagagatga 
tccgcatga agaggaatt tgtagaaca gcaggaaga 
accagaaga acagcaaga agaacctga agaacagca 
agaagaacc tgaagaaca accaccacc accgccaca 
agcagtaaa aagcaaatg ttcgacaaa acaagtgtg 
catcaaaca tgattgtaa gagtggttg caaagcaag 
taaggcttg ttgttcatc agcttctca atcttcttc 
tcctccgcc tcctccttc atcggctcc tcattcttc 
ttccgttcc tccttcatc ggctcgtca ttcttcttc 
ccctcctgc tccatcggc tcggcattc ttcttcccc 
tcctcctcc ttcccaaaa agccggtcc gggagatat 
tacaattgc tattaaaaa gaagcgaaa gggaatagt 
tattgaagg aagagctcc agcgagttg taacaagga 
agtttgtga aatttacaa aaacaaaaa tttggtacc 
ggacaaacc cgagaaaca acaaaaagc taaaagttc 
gtctgacaa gcccccgaa agagcaaaa gttcaaagt 
tgtagccaa agatagtgt agaaataat aataaataa 
caacacgat ttctgtgac aaataacga caaaaggaa 
aaagtaaaa aggcaaata tgtgtttgt acttacgca 
atgatgtgt cataaataa atggtttta tttcgt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY