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Gene information
General information
TC004817 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2298034..2301979
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC004817
      iBeetle screen iB_00770

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC004817 RA
        MDRCFKWPQ ESKSKNYEE DRELIFRLQ SAKRRLSPI 
        VDDIVESAV DLIEKGDDH FVNECTLTP DQYQDVIWA 
        SQMLRQIEY DRMNHNNYY LPNTNLIVN RVSVESVKK 
        TRDKSAVVI DQATVVQLL QLYDQMGVI IQKMLNTNK 
        ATKNVDVQK STDESRSDC RCDTTMPAS TTTTENNEV 
        DSLTSEKSA LKPCIKSTC SAFKKSSSS KENGEDSER 
        KSVSINDEV ETKVCDDPT IYIKIQCYT DCSETAEVD 
        ATPKTSEVK CSKSCDSHY HIKLDGEPV TLKMNQTNH 
        NEDPVFCIN ARSDDESDV KIVIKQCPE CRSTIIGSS 
        TKIENPPES HESLTIHGT PQLDETNLE YSSDDSGKS 
        TSPSECHCK EKKMCIDLA KLKELLGCS CDKPKMDVV 
        IDSDSVEKS EVKVRELPP LMRRRVCSS MEFDVTTVK 
        TRLREKEIE HKNAHIVLE LKQLGVDQI LRASKPAKC 
        CRKAILNER QRTALPYCV SLRSLPLMS HDAEVIKQS 
        VDMSVKKIK NAIDLTQAY KDKLAYYVS LGKDDFKQK 
        QTPWVVPLY DMDLNGEED RFYVLKKEE AEEQVATLL 
        GIVERLRRN DLSYNKNRC FVLRKPNMM RRITEWNES 
        KSSVLSRRI NRKRIKRRM ELKFRNCKP NRRRRSWRS 
        NIDRFGFPH QFNSLDVNF LQTTEISRF TSFAKCSNS 
        GSDIPLNAF PCFNICNLD NKQQEAIVE KSKGSGDCS 
        LSKMLDFSY LNKPEKSKF LTSVFRKKN SKRKKSLPA 
        SNALTIFIP KSKGVKRKK KNVLEYYVG LVKGDLVWS 
        NTLPLVPQN YDTYYNHAS ERKKVPVEE PIVATRVSR 
        AESSREPAR SPSRSSRRS SGSATRRKR LTLPESNNE 
        PQKTVTPSP EPQQVNAPE MAKVDETQE LKDESWFPH 
        VVSETSLPN IQETFEKKE VEQAEAQPV EPRDDPHEE 
        EFVEQQEEP EEQQEEPEE QQEEPEEQP PPPPQAVKS 
        KCSTKQVCI KHDCKSGCK ASKACCSSA SQSSSPPPP 
        PSSAPHSSS VPPSSARHS SSPPAPSAR HSSSPPPPS 
        QKAGPGDIT IAIKKKRKG IVIEGRAPA SCNKEVCEI 
        YKNKNLVPD KPEKQQKAK SSSDKPPKE QKFKVVAKD 
        SVEIIINNN TISVTNNDK RKK
      CDS sequence
      • >TC004817 RA
        atggataga tgtttcaaa tggccacaa gaaagcaaa 
        tctaaaaat tatgaagaa gacagagaa cttatcttt 
        cgtttacaa tcagcaaaa cgcagacta tcacccatt 
        gtggacgat attgttgaa tcagccgta gatctcatc 
        gaaaaaggt gatgatcat tttgtgaac gagtgcaca 
        ctcacacca gaccaatat caagatgta atttgggct 
        tctcaaatg ttacgacag atagaatat gatagaatg 
        aaccacaat aactattac ttaccgaac actaattta 
        atagtgaac cgtgtaagt gtcgaaagc gtgaaaaaa 
        actagagat aagtccgcc gtcgttata gatcaagct 
        acagtagtg cagctattg cagctttac gaccaaatg 
        ggtgttatt atacaaaaa atgttaaac accaataaa 
        gcaaccaaa aatgttgac gttcagaaa tcgaccgat 
        gaaagtcga tcagactgt aggtgtgat accacaatg 
        ccggcctca acaaccacc acagagaac aacgaagtt 
        gattcttta acttccgaa aaaagtgca ctcaaaccg 
        tgcatcaaa tcgacatgc tcagcattc aagaagagt 
        tcttctagc aaagagaac ggcgaggac tcggaaaga 
        aaaagtgtt tcaatcaac gacgaagtg gagactaaa 
        gtttgcgat gaccccacc atctatatc aaaatccag 
        tgctacact gattgctct gaaacggca gaagtcgat 
        gctacgccc aaaaccagc gaagttaaa tgctccaaa 
        agttgcgac tctcattat cacattaaa ctggacgga 
        gaaccagtc acattaaaa atgaatcag accaaccat 
        aatgaagac cctgttttt tgcataaat gcgcgaagc 
        gacgacgaa agtgatgtc aaaattgtg atcaaacaa 
        tgtccagaa tgtagatca acaatcatt ggttcttcc 
        acaaaaatc gaaaacccg ccagaatca cacgagtcg 
        cttacaata cacggaaca ccacaactt gacgaaaca 
        aatctggaa tattcatct gatgattct ggcaaatca 
        acatcaccc tcggaatgt cattgcaag gagaagaaa 
        atgtgtata gacctcgcc aagcttaaa gaactgctc 
        ggttgttca tgcgataaa cccaaaatg gacgtggtt 
        atagattca gacagtgtg gaaaaatca gaagtcaaa 
        gttcgtgaa ctaccacct ttgatgaga cgtcgggtt 
        tgttcctca atggaattc gatgtcacc accgttaaa 
        acgaggctt cgagagaaa gaaatcgag cacaaaaac 
        gcccacatc gtcttagag ttgaaacaa ctaggagtt 
        gaccaaatt ttacgagct agcaaaccg gccaagtgc 
        tgtcggaag gcaattctc aacgaaagg caaagaaca 
        gcgcttcct tactgtgtc agcttgcga tcgttgcca 
        ctgatgagc cacgatgcc gaagtcatc aaacaatca 
        gttgatatg agcgtgaag aaaatcaaa aacgcgatc 
        gatctgacc caagcttac aaagacaaa ctggcgtat 
        tatgtcagc ttggggaaa gatgatttc aaacaaaag 
        caaacaccg tgggttgtg ccactgtac gatatggat 
        ttaaatggc gaagaagac cgcttctac gtgttgaag 
        aaagaagaa gctgaggaa caagttgct acactactt 
        ggaatcgtt gaaagactc cgtcggaac gatttaagt 
        tacaacaaa aatcgatgt tttgttttg aggaaacca 
        aacatgatg agaaggata acggagtgg aatgagtca 
        aaatcgtca gttttgagc aggagaatt aaccggaaa 
        cgcataaaa agacgtatg gaattaaaa ttcagaaat 
        tgtaaacca aacagaagg aggcgaagt tggagaagt 
        aatattgat aggtttggc tttccccac caatttaat 
        tcacttgat gtgaatttt cttcaaacg actgaaatc 
        agtagattt acttctttt gccaaatgt tccaatagt 
        gggtcagat atccccttg aatgctttt ccctgtttt 
        aatatttgt aatttggat aataagcag caagaagca 
        atagttgaa aaaagtaaa ggaagcggt gattgctcc 
        ctaagcaag atgttggat ttctcttat ctaaataaa 
        cccgaaaaa agcaagttc ttaacaagc gtgtttcgt 
        aagaaaaat tcaaaaaga aaaaaatcg ttgcctgct 
        tctaacgct ctaaccatt tttataccc aaatcaaaa 
        ggtgtcaaa cgcaagaaa aaaaatgtt ctagaatac 
        tatgttggt ttagtaaag ggagatcta gtctggagt 
        aacaccctt ccactagta ccccaaaac tacgacacc 
        tattataac catgcaagt gaacgtaag aaagttcct 
        gtggaagag cccatagtc gctactcgc gtttcgcgt 
        gccgaatca agtcgagaa ccagcaagg tcaccttca 
        agaagcagc agaagaagt tctggttct gctaccaga 
        cgcaaacgt ctcacattg ccggaatca aataacgaa 
        ccccaaaaa actgtcaca ccatctcca gaaccccag 
        caagttaat gctccagaa atggctaaa gttgatgaa 
        acacaagaa ctaaaagac gaatcttgg tttcctcac 
        gttgttagc gagacttcc ttgcccaac atccaggaa 
        acatttgaa aagaaagaa gttgaacag gcggaagcg 
        caaccggtg gaaccaaga gatgatccg catgaagag 
        gaatttgta gaacagcag gaagaacca gaagaacag 
        caagaagaa cctgaagaa cagcaagaa gaacctgaa 
        gaacaacca ccaccaccg ccacaagca gtaaaaagc 
        aaatgttcg acaaaacaa gtgtgcatc aaacatgat 
        tgtaagagt ggttgcaaa gcaagtaag gcttgttgt 
        tcatcagct tctcaatct tcttctcct ccgcctcct 
        ccttcatcg gctcctcat tcttcttcc gttcctcct 
        tcatcggct cgtcattct tcttcccct cctgctcca 
        tcggctcgg cattcttct tcccctcct cctccttcc 
        caaaaagcc ggtccggga gatattaca attgctatt 
        aaaaagaag cgaaaggga atagttatt gaaggaaga 
        gctccagcg agttgtaac aaggaagtt tgtgaaatt 
        tacaaaaac aaaaatttg gtaccggac aaacccgag 
        aaacaacaa aaagctaaa agttcgtct gacaagccc 
        ccgaaagag caaaagttc aaagttgta gccaaagat 
        agtgtagaa ataataata aataacaac acgatttct 
        gtgacaaat aacgacaaa aggaaaaag taa
      mRNA sequence
      • >TC004817 RA
        ccgtaattg tagaaatga gattatgaa acaagtaga 
        tattaagta gattatcaa tagattcag aaaatttta 
        gattttcgg tgattatat tcatttcct aggcgttgg 
        tctaaaacg tcgtaaaat gtctagctg acacatgtt 
        tgacatcat ctgattgat cccaatcga atgagtcca 
        caataatca aaatttttg taacatgtc ttaattctt 
        actgaaatt atctgtaat cacacccaa gaacagcag 
        ttctcatca cagaatgga tagatgttt caaatggcc 
        acaagaaag caaatctaa aaattatga agaagacag 
        agaacttat ctttcgttt acaatcagc aaaacgcag 
        actatcacc cattgtgga cgatattgt tgaatcagc 
        cgtagatct catcgaaaa aggtgatga tcattttgt 
        gaacgagtg cacactcac accagacca atatcaaga 
        tgtaatttg ggcttctca aatgttacg acagataga 
        atatgatag aatgaacca caataacta ttacttacc 
        gaacactaa tttaatagt gaaccgtgt aagtgtcga 
        aagcgtgaa aaaaactag agataagtc cgccgtcgt 
        tatagatca agctacagt agtgcagct attgcagct 
        ttacgacca aatgggtgt tattataca aaaaatgtt 
        aaacaccaa taaagcaac caaaaatgt tgacgttca 
        gaaatcgac cgatgaaag tcgatcaga ctgtaggtg 
        tgataccac aatgccggc ctcaacaac caccacaga 
        gaacaacga agttgattc tttaacttc cgaaaaaag 
        tgcactcaa accgtgcat caaatcgac atgctcagc 
        attcaagaa gagttcttc tagcaaaga gaacggcga 
        ggactcgga aagaaaaag tgtttcaat caacgacga 
        agtggagac taaagtttg cgatgaccc caccatcta 
        tatcaaaat ccagtgcta cactgattg ctctgaaac 
        ggcagaagt cgatgctac gcccaaaac cagcgaagt 
        taaatgctc caaaagttg cgactctca ttatcacat 
        taaactgga cggagaacc agtcacatt aaaaatgaa 
        tcagaccaa ccataatga agaccctgt tttttgcat 
        aaatgcgcg aagcgacga cgaaagtga tgtcaaaat 
        tgtgatcaa acaatgtcc agaatgtag atcaacaat 
        cattggttc ttccacaaa aatcgaaaa cccgccaga 
        atcacacga gtcgcttac aatacacgg aacaccaca 
        acttgacga aacaaatct ggaatattc atctgatga 
        ttctggcaa atcaacatc accctcgga atgtcattg 
        caaggagaa gaaaatgtg tatagacct cgccaagct 
        taaagaact gctcggttg ttcatgcga taaacccaa 
        aatggacgt ggttataga ttcagacag tgtggaaaa 
        atcagaagt caaagttcg tgaactacc acctttgat 
        gagacgtcg ggtttgttc ctcaatgga attcgatgt 
        caccaccgt taaaacgag gcttcgaga gaaagaaat 
        cgagcacaa aaacgccca catcgtctt agagttgaa 
        acaactagg agttgacca aattttacg agctagcaa 
        accggccaa gtgctgtcg gaaggcaat tctcaacga 
        aaggcaaag aacagcgct tccttactg tgtcagctt 
        gcgatcgtt gccactgat gagccacga tgccgaagt 
        catcaaaca atcagttga tatgagcgt gaagaaaat 
        caaaaacgc gatcgatct gacccaagc ttacaaaga 
        caaactggc gtattatgt cagcttggg gaaagatga 
        tttcaaaca aaagcaaac accgtgggt tgtgccact 
        gtacgatat ggatttaaa tggcgaaga agaccgctt 
        ctacgtgtt gaagaaaga agaagctga ggaacaagt 
        tgctacact acttggaat cgttgaaag actccgtcg 
        gaacgattt aagttacaa caaaaatcg atgttttgt 
        tttgaggaa accaaacat gatgagaag gataacgga 
        gtggaatga gtcaaaatc gtcagtttt gagcaggag 
        aattaaccg gaaacgcat aaaaagacg tatggaatt 
        aaaattcag aaattgtaa accaaacag aaggaggcg 
        aagttggag aagtaatat tgataggtt tggctttcc 
        ccaccaatt taattcact tgatgtgaa ttttcttca 
        aacgactga aatcagtag atttacttc ttttgccaa 
        atgttccaa tagtgggtc agatatccc cttgaatgc 
        ttttccctg ttttaatat ttgtaattt ggataataa 
        gcagcaaga agcaatagt tgaaaaaag taaaggaag 
        cggtgattg ctccctaag caagatgtt ggatttctc 
        ttatctaaa taaacccga aaaaagcaa gttcttaac 
        aagcgtgtt tcgtaagaa aaattcaaa aagaaaaaa 
        atcgttgcc tgcttctaa cgctctaac catttttat 
        acccaaatc aaaaggtgt caaacgcaa gaaaaaaaa 
        tgttctaga atactatgt tggtttagt aaagggaga 
        tctagtctg gagtaacac ccttccact agtacccca 
        aaactacga cacctatta taaccatgc aagtgaacg 
        taagaaagt tcctgtgga agagcccat agtcgctac 
        tcgcgtttc gcgtgccga atcaagtcg agaaccagc 
        aaggtcacc ttcaagaag cagcagaag aagttctgg 
        ttctgctac cagacgcaa acgtctcac attgccgga 
        atcaaataa cgaacccca aaaaactgt cacaccatc 
        tccagaacc ccagcaagt taatgctcc agaaatggc 
        taaagttga tgaaacaca agaactaaa agacgaatc 
        ttggtttcc tcacgttgt tagcgagac ttccttgcc 
        caacatcca ggaaacatt tgaaaagaa agaagttga 
        acaggcgga agcgcaacc ggtggaacc aagagatga 
        tccgcatga agaggaatt tgtagaaca gcaggaaga 
        accagaaga acagcaaga agaacctga agaacagca 
        agaagaacc tgaagaaca accaccacc accgccaca 
        agcagtaaa aagcaaatg ttcgacaaa acaagtgtg 
        catcaaaca tgattgtaa gagtggttg caaagcaag 
        taaggcttg ttgttcatc agcttctca atcttcttc 
        tcctccgcc tcctccttc atcggctcc tcattcttc 
        ttccgttcc tccttcatc ggctcgtca ttcttcttc 
        ccctcctgc tccatcggc tcggcattc ttcttcccc 
        tcctcctcc ttcccaaaa agccggtcc gggagatat 
        tacaattgc tattaaaaa gaagcgaaa gggaatagt 
        tattgaagg aagagctcc agcgagttg taacaagga 
        agtttgtga aatttacaa aaacaaaaa tttggtacc 
        ggacaaacc cgagaaaca acaaaaagc taaaagttc 
        gtctgacaa gcccccgaa agagcaaaa gttcaaagt 
        tgtagccaa agatagtgt agaaataat aataaataa 
        caacacgat ttctgtgac aaataacga caaaaggaa 
        aaagtaaaa aggcaaata tgtgtttgt acttacgca 
        atgatgtgt cataaataa atggtttta tttcgt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY