General information | |||
TC004817 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 2298034..2301979 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC004817 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00770 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC004817 RA
MDRCFKWPQ ESKSKNYEE DRELIFRLQ SAKRRLSPI VDDIVESAV DLIEKGDDH FVNECTLTP DQYQDVIWA SQMLRQIEY DRMNHNNYY LPNTNLIVN RVSVESVKK TRDKSAVVI DQATVVQLL QLYDQMGVI IQKMLNTNK ATKNVDVQK STDESRSDC RCDTTMPAS TTTTENNEV DSLTSEKSA LKPCIKSTC SAFKKSSSS KENGEDSER KSVSINDEV ETKVCDDPT IYIKIQCYT DCSETAEVD ATPKTSEVK CSKSCDSHY HIKLDGEPV TLKMNQTNH NEDPVFCIN ARSDDESDV KIVIKQCPE CRSTIIGSS TKIENPPES HESLTIHGT PQLDETNLE YSSDDSGKS TSPSECHCK EKKMCIDLA KLKELLGCS CDKPKMDVV IDSDSVEKS EVKVRELPP LMRRRVCSS MEFDVTTVK TRLREKEIE HKNAHIVLE LKQLGVDQI LRASKPAKC CRKAILNER QRTALPYCV SLRSLPLMS HDAEVIKQS VDMSVKKIK NAIDLTQAY KDKLAYYVS LGKDDFKQK QTPWVVPLY DMDLNGEED RFYVLKKEE AEEQVATLL GIVERLRRN DLSYNKNRC FVLRKPNMM RRITEWNES KSSVLSRRI NRKRIKRRM ELKFRNCKP NRRRRSWRS NIDRFGFPH QFNSLDVNF LQTTEISRF TSFAKCSNS GSDIPLNAF PCFNICNLD NKQQEAIVE KSKGSGDCS LSKMLDFSY LNKPEKSKF LTSVFRKKN SKRKKSLPA SNALTIFIP KSKGVKRKK KNVLEYYVG LVKGDLVWS NTLPLVPQN YDTYYNHAS ERKKVPVEE PIVATRVSR AESSREPAR SPSRSSRRS SGSATRRKR LTLPESNNE PQKTVTPSP EPQQVNAPE MAKVDETQE LKDESWFPH VVSETSLPN IQETFEKKE VEQAEAQPV EPRDDPHEE EFVEQQEEP EEQQEEPEE QQEEPEEQP PPPPQAVKS KCSTKQVCI KHDCKSGCK ASKACCSSA SQSSSPPPP PSSAPHSSS VPPSSARHS SSPPAPSAR HSSSPPPPS QKAGPGDIT IAIKKKRKG IVIEGRAPA SCNKEVCEI YKNKNLVPD KPEKQQKAK SSSDKPPKE QKFKVVAKD SVEIIINNN TISVTNNDK RKK
- >TC004817 RA
atggataga tgtttcaaa tggccacaa gaaagcaaa tctaaaaat tatgaagaa gacagagaa cttatcttt cgtttacaa tcagcaaaa cgcagacta tcacccatt gtggacgat attgttgaa tcagccgta gatctcatc gaaaaaggt gatgatcat tttgtgaac gagtgcaca ctcacacca gaccaatat caagatgta atttgggct tctcaaatg ttacgacag atagaatat gatagaatg aaccacaat aactattac ttaccgaac actaattta atagtgaac cgtgtaagt gtcgaaagc gtgaaaaaa actagagat aagtccgcc gtcgttata gatcaagct acagtagtg cagctattg cagctttac gaccaaatg ggtgttatt atacaaaaa atgttaaac accaataaa gcaaccaaa aatgttgac gttcagaaa tcgaccgat gaaagtcga tcagactgt aggtgtgat accacaatg ccggcctca acaaccacc acagagaac aacgaagtt gattcttta acttccgaa aaaagtgca ctcaaaccg tgcatcaaa tcgacatgc tcagcattc aagaagagt tcttctagc aaagagaac ggcgaggac tcggaaaga aaaagtgtt tcaatcaac gacgaagtg gagactaaa gtttgcgat gaccccacc atctatatc aaaatccag tgctacact gattgctct gaaacggca gaagtcgat gctacgccc aaaaccagc gaagttaaa tgctccaaa agttgcgac tctcattat cacattaaa ctggacgga gaaccagtc acattaaaa atgaatcag accaaccat aatgaagac cctgttttt tgcataaat gcgcgaagc gacgacgaa agtgatgtc aaaattgtg atcaaacaa tgtccagaa tgtagatca acaatcatt ggttcttcc acaaaaatc gaaaacccg ccagaatca cacgagtcg cttacaata cacggaaca ccacaactt gacgaaaca aatctggaa tattcatct gatgattct ggcaaatca acatcaccc tcggaatgt cattgcaag gagaagaaa atgtgtata gacctcgcc aagcttaaa gaactgctc ggttgttca tgcgataaa cccaaaatg gacgtggtt atagattca gacagtgtg gaaaaatca gaagtcaaa gttcgtgaa ctaccacct ttgatgaga cgtcgggtt tgttcctca atggaattc gatgtcacc accgttaaa acgaggctt cgagagaaa gaaatcgag cacaaaaac gcccacatc gtcttagag ttgaaacaa ctaggagtt gaccaaatt ttacgagct agcaaaccg gccaagtgc tgtcggaag gcaattctc aacgaaagg caaagaaca gcgcttcct tactgtgtc agcttgcga tcgttgcca ctgatgagc cacgatgcc gaagtcatc aaacaatca gttgatatg agcgtgaag aaaatcaaa aacgcgatc gatctgacc caagcttac aaagacaaa ctggcgtat tatgtcagc ttggggaaa gatgatttc aaacaaaag caaacaccg tgggttgtg ccactgtac gatatggat ttaaatggc gaagaagac cgcttctac gtgttgaag aaagaagaa gctgaggaa caagttgct acactactt ggaatcgtt gaaagactc cgtcggaac gatttaagt tacaacaaa aatcgatgt tttgttttg aggaaacca aacatgatg agaaggata acggagtgg aatgagtca aaatcgtca gttttgagc aggagaatt aaccggaaa cgcataaaa agacgtatg gaattaaaa ttcagaaat tgtaaacca aacagaagg aggcgaagt tggagaagt aatattgat aggtttggc tttccccac caatttaat tcacttgat gtgaatttt cttcaaacg actgaaatc agtagattt acttctttt gccaaatgt tccaatagt gggtcagat atccccttg aatgctttt ccctgtttt aatatttgt aatttggat aataagcag caagaagca atagttgaa aaaagtaaa ggaagcggt gattgctcc ctaagcaag atgttggat ttctcttat ctaaataaa cccgaaaaa agcaagttc ttaacaagc gtgtttcgt aagaaaaat tcaaaaaga aaaaaatcg ttgcctgct tctaacgct ctaaccatt tttataccc aaatcaaaa ggtgtcaaa cgcaagaaa aaaaatgtt ctagaatac tatgttggt ttagtaaag ggagatcta gtctggagt aacaccctt ccactagta ccccaaaac tacgacacc tattataac catgcaagt gaacgtaag aaagttcct gtggaagag cccatagtc gctactcgc gtttcgcgt gccgaatca agtcgagaa ccagcaagg tcaccttca agaagcagc agaagaagt tctggttct gctaccaga cgcaaacgt ctcacattg ccggaatca aataacgaa ccccaaaaa actgtcaca ccatctcca gaaccccag caagttaat gctccagaa atggctaaa gttgatgaa acacaagaa ctaaaagac gaatcttgg tttcctcac gttgttagc gagacttcc ttgcccaac atccaggaa acatttgaa aagaaagaa gttgaacag gcggaagcg caaccggtg gaaccaaga gatgatccg catgaagag gaatttgta gaacagcag gaagaacca gaagaacag caagaagaa cctgaagaa cagcaagaa gaacctgaa gaacaacca ccaccaccg ccacaagca gtaaaaagc aaatgttcg acaaaacaa gtgtgcatc aaacatgat tgtaagagt ggttgcaaa gcaagtaag gcttgttgt tcatcagct tctcaatct tcttctcct ccgcctcct ccttcatcg gctcctcat tcttcttcc gttcctcct tcatcggct cgtcattct tcttcccct cctgctcca tcggctcgg cattcttct tcccctcct cctccttcc caaaaagcc ggtccggga gatattaca attgctatt aaaaagaag cgaaaggga atagttatt gaaggaaga gctccagcg agttgtaac aaggaagtt tgtgaaatt tacaaaaac aaaaatttg gtaccggac aaacccgag aaacaacaa aaagctaaa agttcgtct gacaagccc ccgaaagag caaaagttc aaagttgta gccaaagat agtgtagaa ataataata aataacaac acgatttct gtgacaaat aacgacaaa aggaaaaag taa
- >TC004817 RA
ccgtaattg tagaaatga gattatgaa acaagtaga tattaagta gattatcaa tagattcag aaaatttta gattttcgg tgattatat tcatttcct aggcgttgg tctaaaacg tcgtaaaat gtctagctg acacatgtt tgacatcat ctgattgat cccaatcga atgagtcca caataatca aaatttttg taacatgtc ttaattctt actgaaatt atctgtaat cacacccaa gaacagcag ttctcatca cagaatgga tagatgttt caaatggcc acaagaaag caaatctaa aaattatga agaagacag agaacttat ctttcgttt acaatcagc aaaacgcag actatcacc cattgtgga cgatattgt tgaatcagc cgtagatct catcgaaaa aggtgatga tcattttgt gaacgagtg cacactcac accagacca atatcaaga tgtaatttg ggcttctca aatgttacg acagataga atatgatag aatgaacca caataacta ttacttacc gaacactaa tttaatagt gaaccgtgt aagtgtcga aagcgtgaa aaaaactag agataagtc cgccgtcgt tatagatca agctacagt agtgcagct attgcagct ttacgacca aatgggtgt tattataca aaaaatgtt aaacaccaa taaagcaac caaaaatgt tgacgttca gaaatcgac cgatgaaag tcgatcaga ctgtaggtg tgataccac aatgccggc ctcaacaac caccacaga gaacaacga agttgattc tttaacttc cgaaaaaag tgcactcaa accgtgcat caaatcgac atgctcagc attcaagaa gagttcttc tagcaaaga gaacggcga ggactcgga aagaaaaag tgtttcaat caacgacga agtggagac taaagtttg cgatgaccc caccatcta tatcaaaat ccagtgcta cactgattg ctctgaaac ggcagaagt cgatgctac gcccaaaac cagcgaagt taaatgctc caaaagttg cgactctca ttatcacat taaactgga cggagaacc agtcacatt aaaaatgaa tcagaccaa ccataatga agaccctgt tttttgcat aaatgcgcg aagcgacga cgaaagtga tgtcaaaat tgtgatcaa acaatgtcc agaatgtag atcaacaat cattggttc ttccacaaa aatcgaaaa cccgccaga atcacacga gtcgcttac aatacacgg aacaccaca acttgacga aacaaatct ggaatattc atctgatga ttctggcaa atcaacatc accctcgga atgtcattg caaggagaa gaaaatgtg tatagacct cgccaagct taaagaact gctcggttg ttcatgcga taaacccaa aatggacgt ggttataga ttcagacag tgtggaaaa atcagaagt caaagttcg tgaactacc acctttgat gagacgtcg ggtttgttc ctcaatgga attcgatgt caccaccgt taaaacgag gcttcgaga gaaagaaat cgagcacaa aaacgccca catcgtctt agagttgaa acaactagg agttgacca aattttacg agctagcaa accggccaa gtgctgtcg gaaggcaat tctcaacga aaggcaaag aacagcgct tccttactg tgtcagctt gcgatcgtt gccactgat gagccacga tgccgaagt catcaaaca atcagttga tatgagcgt gaagaaaat caaaaacgc gatcgatct gacccaagc ttacaaaga caaactggc gtattatgt cagcttggg gaaagatga tttcaaaca aaagcaaac accgtgggt tgtgccact gtacgatat ggatttaaa tggcgaaga agaccgctt ctacgtgtt gaagaaaga agaagctga ggaacaagt tgctacact acttggaat cgttgaaag actccgtcg gaacgattt aagttacaa caaaaatcg atgttttgt tttgaggaa accaaacat gatgagaag gataacgga gtggaatga gtcaaaatc gtcagtttt gagcaggag aattaaccg gaaacgcat aaaaagacg tatggaatt aaaattcag aaattgtaa accaaacag aaggaggcg aagttggag aagtaatat tgataggtt tggctttcc ccaccaatt taattcact tgatgtgaa ttttcttca aacgactga aatcagtag atttacttc ttttgccaa atgttccaa tagtgggtc agatatccc cttgaatgc ttttccctg ttttaatat ttgtaattt ggataataa gcagcaaga agcaatagt tgaaaaaag taaaggaag cggtgattg ctccctaag caagatgtt ggatttctc ttatctaaa taaacccga aaaaagcaa gttcttaac aagcgtgtt tcgtaagaa aaattcaaa aagaaaaaa atcgttgcc tgcttctaa cgctctaac catttttat acccaaatc aaaaggtgt caaacgcaa gaaaaaaaa tgttctaga atactatgt tggtttagt aaagggaga tctagtctg gagtaacac ccttccact agtacccca aaactacga cacctatta taaccatgc aagtgaacg taagaaagt tcctgtgga agagcccat agtcgctac tcgcgtttc gcgtgccga atcaagtcg agaaccagc aaggtcacc ttcaagaag cagcagaag aagttctgg ttctgctac cagacgcaa acgtctcac attgccgga atcaaataa cgaacccca aaaaactgt cacaccatc tccagaacc ccagcaagt taatgctcc agaaatggc taaagttga tgaaacaca agaactaaa agacgaatc ttggtttcc tcacgttgt tagcgagac ttccttgcc caacatcca ggaaacatt tgaaaagaa agaagttga acaggcgga agcgcaacc ggtggaacc aagagatga tccgcatga agaggaatt tgtagaaca gcaggaaga accagaaga acagcaaga agaacctga agaacagca agaagaacc tgaagaaca accaccacc accgccaca agcagtaaa aagcaaatg ttcgacaaa acaagtgtg catcaaaca tgattgtaa gagtggttg caaagcaag taaggcttg ttgttcatc agcttctca atcttcttc tcctccgcc tcctccttc atcggctcc tcattcttc ttccgttcc tccttcatc ggctcgtca ttcttcttc ccctcctgc tccatcggc tcggcattc ttcttcccc tcctcctcc ttcccaaaa agccggtcc gggagatat tacaattgc tattaaaaa gaagcgaaa gggaatagt tattgaagg aagagctcc agcgagttg taacaagga agtttgtga aatttacaa aaacaaaaa tttggtacc ggacaaacc cgagaaaca acaaaaagc taaaagttc gtctgacaa gcccccgaa agagcaaaa gttcaaagt tgtagccaa agatagtgt agaaataat aataaataa caacacgat ttctgtgac aaataacga caaaaggaa aaagtaaaa aggcaaata tgtgtttgt acttacgca atgatgtgt cataaataa atggtttta tttcgt
