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Gene information
General information
TC004803 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7464506..7469943
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004803
    iBeetle screen iB_00769

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004803 RA
      MSKNKLNLT LPPGSLDQT PTVTPTSAP FPDAAAPSN 
      NVGGSSIDN LTARIEGLE MDATQRRRI EVFLCQKEK 
      IGGDLSDDD LEKLGELGS GNGGVVIKV RHKSGLIMA 
      RKLIHLEVK QAIKLQIIR ELKVLHECN FAHIVGFYG 
      AFYSDGEIS ICMEYMDAG SLDLILKKA GRIPENILG 
      KITVAVLKG LSYLRDKHA IMHRDVKPS NILINSSGE 
      IKICDFGVS GQLIDSMAN SFVGTRSYM SPERLQGTH 
      YSVQSDIWS LGLSLVEMA IGMYPIPPP DPETLKAMF 
      ESRNDNDSP DHTKGPRPM AIFELLDYI VNEPPPKLP 
      SGLFSDEFK DFVDRCLRK NPDERADLK TLMNHEWIK 
      KAEAEDVDI AGWVCKTME IQPPQNKV
    CDS sequence
    • >TC004803 RA
      atgtcgaag aacaagcta aacctgacc ctccccccg 
      ggttcgctg gaccaaacc cccacggtg acccccaca 
      agtgcgccg tttccggac gccgcagcc ccgtcgaac 
      aacgtcggg ggctccagc atcgacaac ctaacggcc 
      cggatcgag ggcttggag atggacgcc acgcagagg 
      aggcgcatt gaggtgttc ctgtgccag aaggagaaa 
      atcgggggt gatttgagc gacgacgat ttggaaaaa 
      ctgggcgag ttggggtcg gggaacggc ggcgtcgtt 
      attaaagtc cgccataaa tccgggctc atcatggcc 
      cggaaatta atccacttg gaggtgaaa caggcgatc 
      aaactccag ataattcgc gaattgaaa gtcttgcac 
      gagtgcaat ttcgcgcac attgtgggc ttctacggc 
      gcgttttac agcgatggg gagatcagc atctgtatg 
      gagtatatg gacgctggg tctttagac ttaattctt 
      aaaaaagcc ggacggatt cccgaaaat atactcggg 
      aagattacc gttgcggtt ttgaagggg ctgagctac 
      ttgagggat aaacacgcg attatgcac cgcgatgtt 
      aagcccagt aatatactc attaatagt agcggcgag 
      atcaagatt tgtgatttt ggggtctcg ggacaactt 
      atagactcg atggcgaac tctttcgtc ggaacgaga 
      agctacatg tcgccggag agactccaa ggaacgcat 
      tactcagtc caaagtgat atttggtct ctcggttta 
      tcactagtg gagatggca atcggcatg tacccaatc 
      ccacctcct gatccagaa acgttgaaa gcgatgttt 
      gagtcgcga aacgacaac gattcacct gaccacact 
      aaagggcct cgccctatg gcaatcttc gagcttctg 
      gactacatc gtcaacgag cctccgccg aaactgcca 
      agcgggtta ttttcagac gaattcaaa gattttgtt 
      gataggtgt ttaagaaaa aatccggat gaaagggcc 
      gacttgaaa accttgatg aaccacgaa tggatcaaa 
      aaagccgaa gccgaggac gtggacatc gccggttgg 
      gtttgtaaa acaatggaa attcaaccg ccacaaaat 
      aaagtataa
    mRNA sequence
    • >TC004803 RA
      cataattga ctgatgtca gatgttttc tttacttgt 
      gtttttttc tttatttac gtaaaaaca ataagtgta 
      acataaaaa cagtcaaaa cacaaccac tggttacaa 
      tgttcgcgg ttgcagtct cttcgctaa accaaaaat 
      aattctaaa aagttggta gataatgtt gatattttg 
      aggcttttt atctatggg tgtcaacgt cccttccgt 
      taattaatc agtgataca agtgtaata tttgggaga 
      gatgtcgaa gaacaagct aaacctgac cctcccccc 
      gggttcgct ggaccaaac ccccacggt gacccccac 
      aagtgcgcc gtttccgga cgccgcagc cccgtcgaa 
      caacgtcgg gggctccag catcgacaa cctaacggc 
      ccggatcga gggcttgga gatggacgc cacgcagag 
      gaggcgcat tgaggtgtt cctgtgcca gaaggagaa 
      aatcggggg tgatttgag cgacgacga tttggaaaa 
      actgggcga gttggggtc ggggaacgg cggcgtcgt 
      tattaaagt ccgccataa atccgggct catcatggc 
      ccggaaatt aatccactt ggaggtgaa acaggcgat 
      caaactcca gataattcg cgaattgaa agtcttgca 
      cgagtgcaa tttcgcgca cattgtggg cttctacgg 
      cgcgtttta cagcgatgg ggagatcag catctgtat 
      ggagtatat ggacgctgg gtctttaga cttaattct 
      taaaaaagc cggacggat tcccgaaaa tatactcgg 
      gaagattac cgttgcggt tttgaaggg gctgagcta 
      cttgaggga taaacacgc gattatgca ccgcgatgt 
      taagcccag taatatact cattaatag tagcggcga 
      gatcaagat ttgtgattt tggggtctc gggacaact 
      tatagactc gatggcgaa ctctttcgt cggaacgag 
      aagctacat gtcgccgga gagactcca aggaacgca 
      ttactcagt ccaaagtga tatttggtc tctcggttt 
      atcactagt ggagatggc aatcggcat gtacccaat 
      cccacctcc tgatccaga aacgttgaa agcgatgtt 
      tgagtcgcg aaacgacaa cgattcacc tgaccacac 
      taaagggcc tcgccctat ggcaatctt cgagcttct 
      ggactacat cgtcaacga gcctccgcc gaaactgcc 
      aagcgggtt attttcaga cgaattcaa agattttgt 
      tgataggtg tttaagaaa aaatccgga tgaaagggc 
      cgacttgaa aaccttgat gaaccacga atggatcaa 
      aaaagccga agccgagga cgtggacat cgccggttg 
      ggtttgtaa aacaatgga aattcaacc gccacaaaa 
      taaagtata atttatctc gtgtttaac ttatccagc 
      tagtgaatg ttttgtgta ggtatgact tgtttttca 
      gctaaaaac tgtgataaa tttcatttc aagagttgt 
      aaggtttca taatttaac ataagtcgt tttttccca 
      attgaaaaa agtcacaac ccgaaacca tgtcaacat 
      ttattgttt gtattttgt caattaact ggaagaagc 
      gagcgaggt ttgtgtgac gtctgaacg tcgatttat 
      actttttat tgctacaac aactgtaca gtatgctac 
      gacataaaa gtacaaagc tgttctgcc gtatccaaa 
      atcgaaatt gtctgtgat aagttgtac gtgaaaatt 
      ataagtcaa tatgtaatg gttgtggct ttttgcagg 
      actagaact aaacttttt taatttttt attcttaaa 
      ttagttgta actcgcata gtattatag cataagatc 
      gttgattac gttttttca gcttaaaca tgacaacgt 
      gttcaaatt atgaaatgt tatattttc agtgcacat 
      agctaccta cctacacat atagtattt gtttcagtc 
      actttgttt gtaactagt ctggcagaa agttttcgc 
      aaaatcatt agcttgttt catctatat gtctatata 
      tatgtaggt aggctattc gtgaaatga tcaaaaatt 
      ttcccggac tagtggttc atgtttgtt taatgatat 
      tattgaaat aattccatc aaagaaact tgtactctg 
      taaggttga ttaaaaatg tatttgtga aatctatag 
      cttttatgt atgtactat attataata aaattttca 
      aaatagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY