Status
Please wait ...
Gene information
General information
TC004791 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7242924..7260801
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004791
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00767

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature lateral number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature ventral number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    legs femur fused - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs tibiotarsus fused - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs pretarsus sometimes shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs segment shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004791 RA
      MNKLVCCAF VLSLALSCI CDDTLSPPC NSDIYCYGP 
      LLHTIQMER IYEDSKTFV DMKMRFEPN ITLIKFNEF 
      MVINNNKPS KNATRAFVN ENFEPAGQE FEEWDPEDW 
      VKHPKYIDG IQDDEFKQW ALSLNLVWK DLGRKMKKE 
      VELNQSLYS IIWVPHPVI VPGGRFREF YYWDSYWIV 
      QGLLLSEMY GTVKGMLEN FLYIVDKYG HIPNGGRIY 
      YMQRSQPPL MVPMIKLYV DFTNDTHFV RDNIATMEK 
      EFEYWITKH NKTVTLDGK NYTLATYGD RSKGPRPES 
      YSEDVEGAA IFDDNDKKE SFYAELKAA AESGWDFSS 
      RWFIKNATN KGNLTNTKI RSIVPVDLN AMIYWNAVL 
      LSEFNTLLG NLAKVQYYN NIAKEWMEA VTAVLWHEE 
      VGAWLDYDL SNSVKRDYF YPTNIAPLW TGCYNQTDK 
      GKIVRLVLK YLQNKNILY PGGIPTTVE HTGEQWDYP 
      NAWPPLQHI MIVGLNNTG DVVAQRLAF EIAEKWVRS 
      NYKAFKETD AMFEKYDAT VPGGHGGGG EYETQLGFG 
      WTNGIIMDL LYRYSGNLT VEDPPPPSP KPVFEASQD 
      VQSASSFSQ ISAVLIALM ISLTAGFIG VCIYKRRNQ 
      QQTDSRKAP RPSRARYTE LRSIPRRAK STR
    CDS sequence
    • >TC004791 RA
      atgaacaaa ttagtgtgt tgtgcgttt gtgctcagc 
      ctggccctt agttgcatc tgtgatgat actctatcc 
      ccaccttgc aacagcgat atctactgc tacggccct 
      ttacttcac acaattcaa atggaacgc atctacgag 
      gactccaaa accttcgtc gacatgaag atgcgcttc 
      gaaccaaac atcactctg atcaaattc aatgagttc 
      atggtgata aacaacaat aagccgtcc aaaaacgcg 
      acgcgagct ttcgtcaac gagaacttc gagccggcg 
      ggacaagag ttcgaagag tgggatccc gaagattgg 
      gtcaaacat ccgaaatac attgacggg attcaagac 
      gacgagttc aagcaatgg gcgctttcg ctgaatctg 
      gtctggaag gacttgggc cggaaaatg aagaaggaa 
      gtcgaactg aaccaatca ctctacagt attatttgg 
      gtcccgcac cccgtgata gtacccgga gggcgcttc 
      cgcgagttc tactactgg gactcctac tggatcgtg 
      caaggactc ctcttgtcg gaaatgtac gggacggtc 
      aaaggaatg ctcgaaaat ttcctctac atcgtcgac 
      aaatacgga catatcccc aacggggga cgcatctac 
      tacatgcaa agatctcag cctccgcta atggtgcca 
      atgatcaag ctgtatgtt gacttcacg aacgacact 
      cacttcgtt agggacaac atcgcaaca atggagaaa 
      gagttcgag tactggatc accaaacac aacaaaact 
      gtgaccctc gacggcaag aactacacc ctcgcgacg 
      tacggcgac cgctcgaaa gggcctcgc cccgagtcc 
      tactccgaa gacgtcgaa ggcgctgcc attttcgac 
      gacaacgac aagaaggag agtttttac gccgaattg 
      aaagcagcg gccgaatcc ggttgggat ttttcaagc 
      cgttggttt attaaaaac gcgactaat aaaggtaat 
      ttgaccaat accaaaatc cggtctata gtcccagtt 
      gacttgaac gcaatgatc tactggaac gcggttctc 
      ttatctgaa tttaatacc ctcctcggc aatttggcc 
      aaagtccag tattacaac aacattgcg aaagagtgg 
      atggaggcc gtgactgct gttttatgg cacgaagaa 
      gtcggtgcc tggctagac tatgacttg tcaaattca 
      gtgaaacgc gattatttt tacccgact aatattgcg 
      ccactgtgg accgggtgt tacaaccaa acggacaaa 
      gggaaaatc gtgcgtctg gtcttgaag tatttgcaa 
      aataaaaac attctctac cctggtggt atccccacc 
      acggtggaa cacaccggc gaacaatgg gactacccc 
      aatgcctgg cctcccttg caacacatc atgattgtg 
      ggcctgaac aacacggga gatgttgtt gcacaaaga 
      ctagccttt gaaatcgcc gaaaagtgg gtgcgctcc 
      aactacaaa gcgttcaaa gagaccgac gccatgttt 
      gaaaagtac gacgcgacg gtgcctggg ggccacggc 
      gggggcggc gagtacgag acgcagcta gggttcggt 
      tggacgaac gggatcatc atggatttg ttgtaccga 
      tactcgggg aacctgact gtggaggac ccgccacca 
      ccgtcacca aaaccggtc tttgaggcg tcgcaagac 
      gtgcaatct gcgtcgagt tttagccaa atttcggcc 
      gtcttgata gcgttaatg atatcgcta acggcagga 
      ttcatagga gtgtgtatc tacaaacgc cggaaccag 
      cagcagacc gactcgagg aaagcgcct aggccttca 
      agggcacgc tacacggaa ctgcggagc atccctcgg 
      cgtgcgaag agcacgaga taa
    mRNA sequence
    • >TC004791 RA
      ggtgacatg tcacatgtc gcaaagttt gaggttaag 
      tcgccccaa aatgatcaa atcgagtca aaaaagcga 
      tcagtgcgg aatttagcg aaattttcg ctggttcag 
      tgtagtgga tgtgacgtt gtgtcacac gactcgttt 
      ttaattcaa cctcacgcg attttgatc agttttccc 
      caactgggc tctagaatt cgcctaagg cattgcaga 
      gttctagaa acagtgttg ccaaccgcc tcagaacaa 
      ttcccgcag aagatgcaa gcaaaaacc gctgtaatt 
      aatgaaaat cggccactt tttgccaaa tgaatcgaa 
      tttctggag tagatttgg cattcggtt cgtcatgtt 
      ggccgacct gtgtgtgca acgcggcta aatttaaca 
      tacccaact ctcactgct ggaaaaagt gttcgtgca 
      agtgtaccc tccaaaatt cattgtttt atcactgac 
      acagccgac aggtctgta atttgctgt attaaacgt 
      cgcccctca tgtggctat ttataaaaa atcagtgca 
      cgcactaca gattccacc atgtccgtg ggcacataa 
      cgagatcaa aaacgaaat ttttggaaa aattgaagc 
      gaggcgcgt actggaatg atcccgaag agccaacag 
      ctgcattga agaataaat taccgtcaa gatattcaa 
      gaaagtaag agtgatttg tgtgaaatt gaaacttga 
      aataacaga catgaacaa attagtgtg ttgtgcgtt 
      tgtgctcag cctggccct tagttgcat ctgtgatga 
      tactctatc cccaccttg caacagcga tatctactg 
      ctacggccc tttacttca cacaattca aatggaacg 
      catctacga ggactccaa aaccttcgt cgacatgaa 
      gatgcgctt cgaaccaaa catcactct gatcaaatt 
      caatgagtt catggtgat aaacaacaa taagccgtc 
      caaaaacgc gacgcgagc tttcgtcaa cgagaactt 
      cgagccggc gggacaaga gttcgaaga gtgggatcc 
      cgaagattg ggtcaaaca tccgaaata cattgacgg 
      gattcaaga cgacgagtt caagcaatg ggcgctttc 
      gctgaatct ggtctggaa ggacttggg ccggaaaat 
      gaagaagga agtcgaact gaaccaatc actctacag 
      tattatttg ggtcccgca ccccgtgat agtacccgg 
      agggcgctt ccgcgagtt ctactactg ggactccta 
      ctggatcgt gcaaggact cctcttgtc ggaaatgta 
      cgggacggt caaaggaat gctcgaaaa tttcctcta 
      catcgtcga caaatacgg acatatccc caacggggg 
      acgcatcta ctacatgca aagatctca gcctccgct 
      aatggtgcc aatgatcaa gctgtatgt tgacttcac 
      gaacgacac tcacttcgt tagggacaa catcgcaac 
      aatggagaa agagttcga gtactggat caccaaaca 
      caacaaaac tgtgaccct cgacggcaa gaactacac 
      cctcgcgac gtacggcga ccgctcgaa agggcctcg 
      ccccgagtc ctactccga agacgtcga aggcgctgc 
      cattttcga cgacaacga caagaagga gagttttta 
      cgccgaatt gaaagcagc ggccgaatc cggttggga 
      tttttcaag ccgttggtt tattaaaaa cgcgactaa 
      taaaggtaa tttgaccaa taccaaaat ccggtctat 
      agtcccagt tgacttgaa cgcaatgat ctactggaa 
      cgcggttct cttatctga atttaatac cctcctcgg 
      caatttggc caaagtcca gtattacaa caacattgc 
      gaaagagtg gatggaggc cgtgactgc tgttttatg 
      gcacgaaga agtcggtgc ctggctaga ctatgactt 
      gtcaaattc agtgaaacg cgattattt ttacccgac 
      taatattgc gccactgtg gaccgggtg ttacaacca 
      aacggacaa agggaaaat cgtgcgtct ggtcttgaa 
      gtatttgca aaataaaaa cattctcta ccctggtgg 
      tatccccac cacggtgga acacaccgg cgaacaatg 
      ggactaccc caatgcctg gcctccctt gcaacacat 
      catgattgt gggcctgaa caacacggg agatgttgt 
      tgcacaaag actagcctt tgaaatcgc cgaaaagtg 
      ggtgcgctc caactacaa agcgttcaa agagaccga 
      cgccatgtt tgaaaagta cgacgcgac ggtgcctgg 
      gggccacgg cgggggcgg cgagtacga gacgcagct 
      agggttcgg ttggacgaa cgggatcat catggattt 
      gttgtaccg atactcggg gaacctgac tgtggagga 
      cccgccacc accgtcacc aaaaccggt ctttgaggc 
      gtcgcaaga cgtgcaatc tgcgtcgag ttttagcca 
      aatttcggc cgtcttgat agcgttaat gatatcgct 
      aacggcagg attcatagg agtgtgtat ctacaaacg 
      ccggaacca gcagcagac cgactcgag gaaagcgcc 
      taggccttc aagggcacg ctacacgga actgcggag 
      catccctcg gcgtgcgaa gagcacgag ataaacgtg 
      agtgaatga cagttgcgt tgtaattaa gtcaccaaa 
      tcgtcgcac atgcttccg gaggaaact agtattaag 
      tgcctacgt tgacactaa gtagtacaa gtaattaat 
      tttagttaa ttgaatttt gttaatcgg tgtctagaa 
      tagtggcga cttctccat tcttgtatc ttcctatgt 
      gatatctaa gtcatatct ttaagagcg acggcgcgg 
      atttgggca ctgggtgca aagactcga gtgattcat 
      ttatttatt tgttcaaac ttttgtgat aatgcaaca 
      tgcccagat ccacacttt taaagatca aagcaggtt 
      tagggttcg cctccagtg atcaaacca tgtatagtt 
      ttatttatt tagttgaat agcgaaact catgacgta 
      catttctag acttacctc agtagacaa aatcatcca 
      atgccaatt tgtgtctta aggaaactc caaattctg 
      caacgcata atgtattga tatgtgtac ataggtgtg 
      ctacatgta ttaaaagca tttgctgtg attaagtcg 
      agctaaaat atatttgat aaataactg tgtgaaata 
      aaagatttg gaaaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY