General information | |||
TC004781 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 6975909..6985239 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC004781 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00765 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)labrum not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
head cuticle partially protruding - Penetrance (percentage of animals/eggs on slide): less than 30%
procephalic head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
- >TC004781 RA
MADDDAVES KKSEEKPSE SATTEPKKK ITVTVKTPK EKENFEVDE DLAVKAFKE VIAPVFNAD VDQLCLIFA GKIMKDNDT LQQHNIKDG LTIHLVIRA VPRSTESGP SRPPADVSA TPFNLGPLG GLAGLDSLG VGSGNFMEI QSRMQTELL NNPDMLRQL LDNPFVQRI MNDPENMRA LITRNPQMQ ELMERNPEI NHMLNNPEL LRQTMELAR NPSMLQELM RSHDRAISN LESIPGGYN ALQRMYRDI QEPMFSATS EQFSRNPFA GLVDNNQSG NNPQQGTEN REPLPNPWG GQRPNTNNS STNRPQRPV MNSPSMASL LQQMSENSD LMQNMLSAP YTQNVLEAL ASDPNMATA LLSDNPLLS GNPQLQEQV RTMIPSFVQ QLQNPDIQN VMSNPQALN AIMQIHQGM ETLRQTAPN LVNTFGAGG TTATTTPST TTTTTTTTT TQANTTTTQ SSTNSDPFS EFMARMVAG MASQRDTTL PPEQRYQTQ LEQLASMGF VNREANLQA LIATFGDVN AAVERLLAL GQLSMS
- >TC004781 RA
atggccgac gatgatgcg gttgagtca aagaaaagt gaggaaaaa cccagtgaa agcgccaca acggagcca aaaaagaaa ataacggtg acggtgaag acgcccaaa gaaaaggaa aacttcgaa gtggacgaa gaccttgct gtgaaggct tttaaggag gtcatagct ccggtgttt aatgcggat gttgaccaa ttatgccta atcttcgcc gggaaaatc atgaaagac aatgacacc ttgcagcag cacaatatt aaagacggc ctcaccata catctggtc atcagggct gtgccccgc agcaccgag tcggggccc tcgcgcccg ccagcggac gtaagcgca accccgttt aatttgggt cctttgggt ggtttggcg ggtttggat tcgttgggt gtgggttct ggcaatttc atggaaatt caaagtcgg atgcagact gagctttta aataatcct gacatgcta agacaactc ttagacaat ccatttgtc caacgaata atgaacgat ccagaaaac atgcgagcc ctaataacc cgcaacccc caaatgcag gaactaatg gagcgcaac cccgaaatc aaccacatg ttaaacaac cccgaattg ctgcgtcag acgatggaa ctcgcccgt aacccgtca atgttgcag gagttgatg cggagccac gacagggct atcagcaac ctggagagc atccccggg ggctacaac gccctccag cgcatgtac agagacatc caggagccg atgttttca gcaactagc gaacagttc agtcgtaat cctttcgcc ggtcttgtt gataataac caaagtggg aataacccc cagcaaggg acggaaaac cgggagccc ctccccaat ccctggggc ggtcagagg cccaacacg aataatagc tcaacgaat aggccccaa agaccggtc atgaacagc ccttcgatg gcaagtttg ctgcaacaa atgtccgaa aattcagac ttgatgcaa aatatgctg tcagcacct tatacgcaa aatgtactc gaggcctta gctagcgac ccaaatatg gcaacggcg ctcctttct gataacccc ctgctttcg ggaaaccca caactgcag gaacaagtc aggacgatg attccctcg tttgtgcaa caactgcaa aatcccgac atacagaac gtaatgtcg aacccacag gctctcaat gcgattatg caaatccac caagggatg gaaacgctt cggcagacg gccccaaac ctcgtaaat actttcggc gccggtggc acgactgca accacaacg ccatctaca acaacaaca acaactact acgactacg actcaggca aacacaaca acgactcaa tctagcacg aattcagac ccgttttca gagtttatg gcccgaatg gttgcgggg atggcatct cagcgtgat acaacttta cctccagag cagcggtac caaacacag ttggaacag ttggcctcg atgggcttt gtaaataga gaagcgaat ctgcaagct ttaattgcc acttttggc gatgtcaat gctgcagtg gagaggcta ctagcgctc gggcagtta tccatgagc taa
- >TC004781 RA
agctgttcc aatcttttt gtttgagac aaccagaaa tacaccggt gtcacaaag ttgcgttat ttgaatgca agtggaaaa cacccagtg ggggaatgt tgacatttg ggtacacaa tagtggcag cattgtcga gtaaattta tttggtgat ttggttttc agtgtggtg aacctttag tcaggcaaa catggccga cgatgatgc ggttgagtc aaagaaaag tgaggaaaa acccagtga aagcgccac aacggagcc aaaaaagaa aataacggt gacggtgaa gacgcccaa agaaaagga aaacttcga agtggacga agaccttgc tgtgaaggc ttttaagga ggtcatagc tccggtgtt taatgcgga tgttgacca attatgcct aatcttcgc cgggaaaat catgaaaga caatgacac cttgcagca gcacaatat taaagacgg cctcaccat acatctggt catcagggc tgtgccccg cagcaccga gtcggggcc ctcgcgccc gccagcgga cgtaagcgc aaccccgtt taatttggg tcctttggg tggtttggc gggtttgga ttcgttggg tgtgggttc tggcaattt catggaaat tcaaagtcg gatgcagac tgagctttt aaataatcc tgacatgct aagacaact cttagacaa tccatttgt ccaacgaat aatgaacga tccagaaaa catgcgagc cctaataac ccgcaaccc ccaaatgca ggaactaat ggagcgcaa ccccgaaat caaccacat gttaaacaa ccccgaatt gctgcgtca gacgatgga actcgcccg taacccgtc aatgttgca ggagttgat gcggagcca cgacagggc tatcagcaa cctggagag catccccgg gggctacaa cgccctcca gcgcatgta cagagacat ccaggagcc gatgttttc agcaactag cgaacagtt cagtcgtaa tcctttcgc cggtcttgt tgataataa ccaaagtgg gaataaccc ccagcaagg gacggaaaa ccgggagcc cctccccaa tccctgggg cggtcagag gcccaacac gaataatag ctcaacgaa taggcccca aagaccggt catgaacag cccttcgat ggcaagttt gctgcaaca aatgtccga aaattcaga cttgatgca aaatatgct gtcagcacc ttatacgca aaatgtact cgaggcctt agctagcga cccaaatat ggcaacggc gctcctttc tgataaccc cctgctttc gggaaaccc acaactgca ggaacaagt caggacgat gattccctc gtttgtgca acaactgca aaatcccga catacagaa cgtaatgtc gaacccaca ggctctcaa tgcgattat gcaaatcca ccaagggat ggaaacgct tcggcagac ggccccaaa cctcgtaaa tactttcgg cgccggtgg cacgactgc aaccacaac gccatctac aacaacaac aacaactac tacgactac gactcaggc aaacacaac aacgactca atctagcac gaattcaga cccgttttc agagtttat ggcccgaat ggttgcggg gatggcatc tcagcgtga tacaacttt acctccaga gcagcggta ccaaacaca gttggaaca gttggcctc gatgggctt tgtaaatag agaagcgaa tctgcaagc tttaattgc cacttttgg cgatgtcaa tgctgcagt ggagaggct actagcgct cgggcagtt atccatgag ctaacctat tcatatatt ccaactgta ttattatta cttttaaat tttgggctt tttaaaata ttttgttta ggatagact actttatct atgtattag acattcgat tttttctta ctgggttgt atttggtgc cacttgttt tcattgttt ttgccatta ttgtgaaca gctcgcttc aaattgtac agggcgagt ttcctgagt atttttttc tccacctcc gagtataat aatcacagg aaccagcgt tcgttttaa atacttttt attttctac gaaaaaagt atttaatag caaacgtgg tttctgtgt gttcgtttt tgaggtgca gtgaggtac ctttagcag actaaccct gtatatgtg tttgaaggg actgtagga cacggaatt aatccaatg ttgatttga cttggaaaa tgtatatct ataattaat tagagacta ttttagcaa cacgtaaag tattttatt atcgacttt aataattaa cctttcata gaaatattg caatcatgg caaacacta gtcagatgg atggtttgt cagcaaaaa cgactgatc tgtggcttg tgtaaaaca atcaaatga tattatcag ttgatgtat tatatgtta tatgggtgt ctaattttg agaaatctt atattttta atatattgt ttttgttga ggaaattcg ttgttaata tttttggtt tgagaggag tgattacca actgtgcaa attttaaat tttatactg ctaatacaa tggcaataa cactagagt tatgtgtta agtggctca aacaatatt ctctgtaat ttattatag ctgtcagtg tttctgagg cgacactgg ttcatggag actacgacc acagatcgc tttttcttc aaactaaac gaactcata gctaataga catacaatt ttttgtgca ttgtacatt ccatactgt aagacaact cgcataata aatcagttt ctccatg
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
