Status
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Gene information
General information
TC004751 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6373096..6376497
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004751
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00762

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004751 RA
      MNERKNQLF RVQVSVRQR PYTKDEIEL KKTSIVKVS 
      NQNELTLQC GSNGKNYTF DRVFKSGAS QAEVYDVVV 
      RPLVDSMLS GCTCTVFAY GPTGTGKTY TMIGDSVRP 
      TLDFLNDPS VGMVPRAAA DIFNRLSQL GVQFNVTVS 
      FVEIYNEEV RDLLISDTC NLRIYDDPD NKGATCIKG 
      VSEVPVKNC TELFELLMI GASDRHMAS TNLNRQSSR 
      SHTIFTIVA TIRELSNGV ETIKTGKIN LIDLAGSEN 
      IGRSGATEM RAREAGTIN KSLLTLAKV IKALALKSQ 
      HIPYRESKL TRILQDSLG GKSKTSIIT TFSPSPDVF 
      EETISTLDY AHMARTVSN CPTYNITTI RRDHLTKEL 
      ESEVKSLRL ELNAARTGD GFYVDIETY EKLHLDAKV 
      KHERVVTLL SEITKLDGV IKYQTQCSD ELGKNYQES 
      VTKLEAVTK ELEQETIKQ KESEYAITR MSDKLSQLE 
      EQSKVLRDV CHTSTKHGD IYYQKYDSQ CDATLHKAK 
      IAKEMVAVS TTNLRGIGN ALEEFGEKE RTCLDEIAT 
      SNAHVKHNV LNDLSRLNR LLNDVAAFS SSLTCTPET 
      LLGNTEFTE SGARATLKS VHTDNYALM VQYFAKIRT 
      TLSNLKGHV DDIKASVAQ AALLYHDNV ASQFSLLSD 
      VFDKFYSLM HRLYVGMQK RHARIQGSI KRSDESIAS 
      WEKLIELNA ATCKDAKLE LESISHMRE YIDEMTELL 
      RERMPAVDH NLNTFFCNF NEQTAHPLL NHLRLRHEA 
      AGYEIGESQ ATCLEQAQQ HYIRNQKYM VGFFTAIAK 
      GLEVHNKNV AKGRKALVE EAESILENQ QRVTAQSSK 
      AVASSLSNL ALNEDFYLA NTVKVSENT VENLEQKLV 
      EVDFTIKTG DTPVKPTYD YPKKIDSVL SKEVLKREF 
      KKSLLQEKQ DNSNESTDG DK
    CDS sequence
    • >TC004751 RA
      atgaatgaa cgcaaaaac caactattt cgcgtccag 
      gtcagcgtt agacaacga ccttacact aaagatgaa 
      attgagctg aagaaaact agcattgtg aaagtttca 
      aatcagaac gaactcacc ctccagtgt ggctcaaat 
      ggcaaaaac tacactttt gaccgagtt ttcaagtct 
      ggagcttcg caagccgaa gtttacgat gttgttgtg 
      cggcctttg gttgactcg atgctgtcg gggtgcacg 
      tgcactgtc ttcgcctat gggcccacg ggaaccggc 
      aagacgtac accatgatc ggggactca gtcagaccc 
      acacttgat tttctcaat gacccgtct gttggtatg 
      gtcccacgg gctgctgct gatattttc aatagactg 
      tctcaactg ggggtccag tttaacgta actgtttca 
      tttgttgag atatacaat gaagaagtt cgcgatctt 
      ttaatttca gacacttgc aatctgaga atttatgat 
      gatccggac aacaagggg gcaacctgt atcaagggt 
      gtgtctgaa gtaccggtg aaaaattgc accgaactt 
      tttgaattg ttgatgatc ggagccagc gatcgccac 
      atggctagc actaactta aaccgtcag agttcacgc 
      tctcacacc atttttaca attgtcgcc actattcgc 
      gagttgtcc aacggggta gagaccatc aaaacgggt 
      aaaatcaat ctgatcgac ttagccggg agtgagaac 
      atcggccgt tctggggcc accgaaatg cgggccaga 
      gaagcgggg accatcaac aagtcactc ctaactttg 
      gccaaagtc attaaagct ctcgccttg aagtcccaa 
      catatcccc taccgggaa tcgaaactg acgcgaatt 
      ttgcaagac agtttaggc ggcaagagc aaaacgtcc 
      atcattacg accttttcg ccgtctccc gacgttttc 
      gaagagact atcagtact ctggactac gcacacatg 
      gcccgaacc gtctctaac tgccccacg tacaacata 
      acaaccata cgaagggac cacctgacc aaagaactg 
      gaaagcgaa gtcaagtcg ctacgtttg gaattaaac 
      gccgccagg acaggagac ggcttttac gtggacatc 
      gaaacctat gagaagctg catcttgac gctaaagtc 
      aagcacgaa agagttgtt acgctttta tcggaaata 
      acaaagtta gatggtgtc atcaaatac caaacgcag 
      tgttctgac gaactgggt aaaaactac caggagtcc 
      gtgaccaaa ctggaggct gtcaccaaa gaattggaa 
      caagaaacg atcaagcag aaggagagt gaatatgcc 
      atcacacgc atgagcgac aagctttca caactggag 
      gagcaatcg aaagtcttg cgcgacgtt tgccacacg 
      tcaacaaaa catggagat atctattat cagaagtac 
      gactcccag tgtgatgct acgctacat aaggccaaa 
      atcgccaaa gaaatggtc gcggtttca accacgaac 
      ctgcgaggc attggcaac gcgcttgaa gagtttggg 
      gaaaaagaa cgaacttgt ttggatgaa attgccaca 
      agcaatgcg cacgttaag cacaatgtt cttaacgac 
      ttgagccgt ttgaaccgt cttctgaac gacgttgcg 
      gccttttcc tctagtctt acatgcacg cctgagacg 
      ctcctcggg aacacggag ttcaccgag agtggggcg 
      agggcaact ttgaagtcc gtacatacc gacaattat 
      gcactgatg gtgcagtat tttgccaaa atcaggacg 
      actttgagt aaccttaag ggccatgtg gacgatatc 
      aaggcatca gtggcccaa gcggcgctt ctgtaccat 
      gacaacgtc gcttcgcag ttttccttg ctgagcgac 
      gtcttcgac aaattctac tccttaatg caccgtctg 
      tatgtaggc atgcagaaa cgccacgcc cgaattcag 
      ggctcaatc aagcgctct gatgaaagc atcgcatcg 
      tgggagaag ttgatcgag ctgaatgcc gccacatgc 
      aaggacgcg aagctcgaa ttggagagt atatcgcac 
      atgagagag tacattgat gaaatgacc gagctgtta 
      agggagcga atgccagca gtggaccac aacttaaat 
      acgttcttt tgcaacttc aacgaacag acggcgcat 
      cctctgcta aatcacctg agactgagg catgaggct 
      gcgggctac gaaattggg gaaagccaa gcgacttgc 
      cttgagcag gcgcaacaa cattatata cgcaatcaa 
      aagtacatg gttgggttc tttacagcg attgcaaag 
      ggcttggag gttcataat aagaacgtt gccaaaggg 
      agaaaagca ctggtggag gaggctgaa agcattcta 
      gaaaatcaa caacgcgtg actgcacag tcctccaaa 
      gccgttgca agttcactt agcaatcta gctctgaac 
      gaagatttt tacctggca aatacggtc aaagtttcc 
      gaaaacacg gttgaaaac ctcgaacag aagttagta 
      gaggttgat tttacgatt aaaacgggt gatacaccg 
      gttaagcca acgtacgat tatccgaaa aagattgat 
      tctgtgttg tcgaaggaa gtattgaaa agggagttt 
      aagaagtct ttgctacaa gagaagcaa gataactcg 
      aatgagtcc acggatggt gataaatga
    mRNA sequence
    • >TC004751 RA
      taatatcga caacccaca atacaaaac ttacggttt 
      tcgttagtg tatttcacg gcctcagat tggcacgtt 
      cgaagagcg aaaaatttg taataacgt tcgcgctgt 
      gtgtttgtt gattgttcg ccgattttc tgaaagaaa 
      ataactcaa aaaagtggt ttaataact tacattcgt 
      cttcccaca atgaatgaa cgcaaaaac caactattt 
      cgcgtccag gtcagcgtt agacaacga ccttacact 
      aaagatgaa attgagctg aagaaaact agcattgtg 
      aaagtttca aatcagaac gaactcacc ctccagtgt 
      ggctcaaat ggcaaaaac tacactttt gaccgagtt 
      ttcaagtct ggagcttcg caagccgaa gtttacgat 
      gttgttgtg cggcctttg gttgactcg atgctgtcg 
      gggtgcacg tgcactgtc ttcgcctat gggcccacg 
      ggaaccggc aagacgtac accatgatc ggggactca 
      gtcagaccc acacttgat tttctcaat gacccgtct 
      gttggtatg gtcccacgg gctgctgct gatattttc 
      aatagactg tctcaactg ggggtccag tttaacgta 
      actgtttca tttgttgag atatacaat gaagaagtt 
      cgcgatctt ttaatttca gacacttgc aatctgaga 
      atttatgat gatccggac aacaagggg gcaacctgt 
      atcaagggt gtgtctgaa gtaccggtg aaaaattgc 
      accgaactt tttgaattg ttgatgatc ggagccagc 
      gatcgccac atggctagc actaactta aaccgtcag 
      agttcacgc tctcacacc atttttaca attgtcgcc 
      actattcgc gagttgtcc aacggggta gagaccatc 
      aaaacgggt aaaatcaat ctgatcgac ttagccggg 
      agtgagaac atcggccgt tctggggcc accgaaatg 
      cgggccaga gaagcgggg accatcaac aagtcactc 
      ctaactttg gccaaagtc attaaagct ctcgccttg 
      aagtcccaa catatcccc taccgggaa tcgaaactg 
      acgcgaatt ttgcaagac agtttaggc ggcaagagc 
      aaaacgtcc atcattacg accttttcg ccgtctccc 
      gacgttttc gaagagact atcagtact ctggactac 
      gcacacatg gcccgaacc gtctctaac tgccccacg 
      tacaacata acaaccata cgaagggac cacctgacc 
      aaagaactg gaaagcgaa gtcaagtcg ctacgtttg 
      gaattaaac gccgccagg acaggagac ggcttttac 
      gtggacatc gaaacctat gagaagctg catcttgac 
      gctaaagtc aagcacgaa agagttgtt acgctttta 
      tcggaaata acaaagtta gatggtgtc atcaaatac 
      caaacgcag tgttctgac gaactgggt aaaaactac 
      caggagtcc gtgaccaaa ctggaggct gtcaccaaa 
      gaattggaa caagaaacg atcaagcag aaggagagt 
      gaatatgcc atcacacgc atgagcgac aagctttca 
      caactggag gagcaatcg aaagtcttg cgcgacgtt 
      tgccacacg tcaacaaaa catggagat atctattat 
      cagaagtac gactcccag tgtgatgct acgctacat 
      aaggccaaa atcgccaaa gaaatggtc gcggtttca 
      accacgaac ctgcgaggc attggcaac gcgcttgaa 
      gagtttggg gaaaaagaa cgaacttgt ttggatgaa 
      attgccaca agcaatgcg cacgttaag cacaatgtt 
      cttaacgac ttgagccgt ttgaaccgt cttctgaac 
      gacgttgcg gccttttcc tctagtctt acatgcacg 
      cctgagacg ctcctcggg aacacggag ttcaccgag 
      agtggggcg agggcaact ttgaagtcc gtacatacc 
      gacaattat gcactgatg gtgcagtat tttgccaaa 
      atcaggacg actttgagt aaccttaag ggccatgtg 
      gacgatatc aaggcatca gtggcccaa gcggcgctt 
      ctgtaccat gacaacgtc gcttcgcag ttttccttg 
      ctgagcgac gtcttcgac aaattctac tccttaatg 
      caccgtctg tatgtaggc atgcagaaa cgccacgcc 
      cgaattcag ggctcaatc aagcgctct gatgaaagc 
      atcgcatcg tgggagaag ttgatcgag ctgaatgcc 
      gccacatgc aaggacgcg aagctcgaa ttggagagt 
      atatcgcac atgagagag tacattgat gaaatgacc 
      gagctgtta agggagcga atgccagca gtggaccac 
      aacttaaat acgttcttt tgcaacttc aacgaacag 
      acggcgcat cctctgcta aatcacctg agactgagg 
      catgaggct gcgggctac gaaattggg gaaagccaa 
      gcgacttgc cttgagcag gcgcaacaa cattatata 
      cgcaatcaa aagtacatg gttgggttc tttacagcg 
      attgcaaag ggcttggag gttcataat aagaacgtt 
      gccaaaggg agaaaagca ctggtggag gaggctgaa 
      agcattcta gaaaatcaa caacgcgtg actgcacag 
      tcctccaaa gccgttgca agttcactt agcaatcta 
      gctctgaac gaagatttt tacctggca aatacggtc 
      aaagtttcc gaaaacacg gttgaaaac ctcgaacag 
      aagttagta gaggttgat tttacgatt aaaacgggt 
      gatacaccg gttaagcca acgtacgat tatccgaaa 
      aagattgat tctgtgttg tcgaaggaa gtattgaaa 
      agggagttt aagaagtct ttgctacaa gagaagcaa 
      gataactcg aatgagtcc acggatggt gataaatga 
      tgcgacata gcatgacct tatttattt tgttcacta 
      gttctagtt tgttatttt taataattt gtgtgcaat 
      gta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY