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Gene information
General information
TC004742 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6296636..6302543
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004742
    iBeetle screen iB_00761

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    remarks: later than schedule,
    Pupal morphology
    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004742 RA
      MSSSTPFWR KIHQTISGH SQNITEDIV AEILKLSKT 
      ELLEGLLSY RPYTKKGYH DWVAVSDVS ICMKEFVHD 
      ISQVLNLDT ITAYPVMCN YLMFEYYGK IKEFWQITR 
      MGSTLHLKE SIWNFYTAE RMFLLKTWR HIFECVNKD 
      YEFAEQYKD FLKTIKIEE LQNNLITQF EELLNEVTS 
      NNFDERSPY NENWLCRNI REQIEILLI LILTATHKP 
      LATAKLSHL IKLFTQNNF GTQPAFFDI RTYAKPQDL 
      DQLMFAEVG CYFAILDNY WDKEIPDFW KQDSTSELD 
      KSLLNLHLN PGHIIIMLA WLTLKFKSL NRDQDAEKI 
      LELFKRQPF ILWHKILSD SMFKNCTVG EVVLRATHK 
      MLDELCLMF DDRKILYED GGVIKILAE FLKQPDLAE 
      ICLQTRNGL DCMTEIAIE SFPHDFYSF TTIAQSLLG 
      LRDQYKQTM QLLNNFPSF LSEMTWTIP KEQISVLRK 
      NQPAFPDSD LIVFPPKTV VRSYRIFGK NLIQYKGKY 
      SYFFHLEYL VTTLINNGL CHRKFDEAL VQKVIAGYG 
      VVIQAIRYC VDMDLRTVG LERIILKLN KLLVHLDGP 
      LLHFELVKL YFDLQNALM LYRQSSFFK ICSEIVWKI 
      FFPKLKPEY LTQDVKILL LNQYVFEDR IFMQLFQKE 
      EFKEDHTLL LTYIKILSN IIKVKFQFK NFQLSGVVY 
      LFNYVFLQH QTWVYKDEN QKTQITLEC LKIFHYVLQ 
      KQVEDLTDI ELKIFQLCN HAFLHNVYV IDSFLRLFV 
      KERYYLIYA MERESNWHQ GPSLDTLQC IRMQLALLL 
      LIMNRKKAI GDCCFSERL PFVVKPVAS YFTNAYSPL 
      IAELSCRFL EKLSQDPFV PLLALLELD HYQVQSLFL 
      ERLRDPLEE ENVKMAIID LINTCISSQ DGMTAAFFN 
      LKCFMYWDG AENDVINGD SVSDFMVDY LQNIKKSHE 
      YFKNPLQLG ILRLLFNLW LNHRENLIE NIASLKDFW 
      PVMADPFFC DYKQDIEIY TIILRIINL EIGANIDKV 
      DEKLVKTID KFLKDKKRI AQWNKFVLA SSQSSAKNK 
      LDLLSAWME FLVLTKKVI PDSFENDVK FALVACCLD 
      GLHPPEGGF SNIETIYLW SQLYLLLIS TWSVYEKKE 
      QVVLGKLQT FLHALNTYY KYLTPKIKE NILCAVNRT 
      ILDLQEYFS VNTAQLLTF LYSVGPLID AEYYYLVSE 
      AFETEDDAE KVRRLKPWL IIVFIGNSI MALDRCEEI 
      GLWFYYEQF LQRVMDSVG SMMEHSATL PFAKLAIDF 
      LITYAESPF VKDFLKEDL FSFYFKVRP PPLTISVGE 
      AILSGLPVN LKEWWFILI TLIKLNRIL IQQIGDAML 
      RTCFTFITQ HDLLLKEIV SLTKYTVDM SALTLVCET 
      LKLILVVLT QMPHWRVDS PYSYNLIME GIKTTIHAC 
      VLSILGYKK INFYDIING KNLNIIDVT PTDLLVSVL 
      NKLTEITYW GCSCLKKTK TDLVTQLNA HYETENLAL 
      LLVENDFSV PRFELPITP RLTYGSLLC LADYLCKTL 
      NQLSPATPS RISRSRQVS LTDLNNQMK VKSLGKIFE 
      HCTATYEEF IGFMVYIQK SDDLPNEKF SSFLHYDMT 
      EFSGTTSAV SQLDYHLVK NALEALMSF LAGEIFFAI 
      RNLRGEAVF AYRRELSSE IQFFYEFVR KRTSEQYSA 
      MVATPRSTS SIAQTEPDL IKRYIITKV MDNDTSDIV 
      DNNFVLVIS QWLIKFCHL SN
    CDS sequence
    • >TC004742 RA
      atgtcttct tccacccct ttttggaga aaaatccac 
      caaacaata tcgggccat agccaaaac attactgaa 
      gacatagtc gcggaaatt ttaaaactc tcgaaaaca 
      gaattactt gaaggcctc ttgagctat cgaccgtac 
      acaaaaaag ggttatcat gactgggtt gcggtctcg 
      gacgttagc atttgcatg aaagaattc gttcatgat 
      ataagccaa gttcttaat ctggacaca ataacggct 
      tatccggtc atgtgcaat tacctgatg tttgagtat 
      tatggaaaa attaaggag ttttggcaa ataactaga 
      atgggttca accttacat ttgaaagag agtatttgg 
      aatttttac acggctgaa aggatgttt ttgctgaag 
      acgtggaga cacatattt gagtgtgtc aataaggat 
      tatgagttt gctgagcag tacaaggat tttctaaaa 
      acgatcaaa atcgaggaa ttgcaaaat aacttgatt 
      acgcagttt gaggagctc cttaacgag gtcacgtct 
      aataatttt gatgagagg agtccgtac aatgaaaac 
      tggctgtgt cggaacatc agggaacaa atagaaatt 
      ctcctgatt ctcatccta acagccacc cataaaccc 
      ctggcaact gcaaaactc tctcatttg attaagtta 
      ttcactcag aataatttc ggcacccag ccggcattt 
      ttcgacatc cggacgtac gcaaaaccg caagacttg 
      gaccagtta atgtttgcc gaagtcggg tgttacttt 
      gccattttg gacaattat tgggataag gaaattcca 
      gatttttgg aaacaagac tcaacaagc gagttggac 
      aagtcgctg cttaactta cacctcaac ccagggcac 
      attataata atgctggcc tggttaact ctgaaattt 
      aaatcgcta aatcgtgac caagacgcc gaaaaaata 
      ctagagctg ttcaaaaga cagccgttt attttgtgg 
      cacaaaatc ctctcagat agtatgttt aagaactgt 
      acggttgga gaagtcgtt ttgcgagca acgcataaa 
      atgctcgac gagctttgt ttgatgttc gacgaccga 
      aaaattctc tacgaggac ggcggcgtg attaaaatc 
      cttgccgag tttttgaaa caaccagac ttggccgaa 
      atttgtttg caaacgcga aatggttta gactgcatg 
      acggaaatc gcgattgag tcatttcct cacgatttt 
      tattcgttc accaccatt gcacaatcg ctgctaggc 
      ctgcgtgac caatacaaa caaaccatg caattgttg 
      aataatttc ccgtcgttt ttgtccgaa atgacctgg 
      accattcca aaggaacaa atctcggtt ttgcgcaaa 
      aaccagccg gcgtttccc gacagcgac ctgatagtc 
      ttcccgccg aaaacggtg gtgcggtcg taccgaatt 
      ttcggaaaa aacctgata cagtacaag gggaagtac 
      agctacttc ttccacctc gagtatttg gtcacaacg 
      ctgataaac aacgggctg tgccaccgc aagtttgac 
      gaggctttg gtccagaag gtcattgcc gggtatggc 
      gttgtgatc caagctatt aggtattgc gtggacatg 
      gacttgaga acagtgggg cttgagaga attattctg 
      aaattaaac aaactccta gtgcatttg gacggccct 
      ttgctgcac tttgagttg gtcaaactt tacttcgac 
      ttgcaaaat gcgctaatg ctgtaccga caaagcagc 
      ttcttcaag atctgctcc gaaatcgta tggaaaata 
      tttttcccg aagctcaaa ccggaatat ttgacccaa 
      gacgttaaa attttgctg ctgaatcag tacgtgttt 
      gaggaccga attttcatg caattgttc caaaaagag 
      gagtttaag gaagatcac actctcctt cttacctac 
      atcaagatt ctaagcaac attattaag gtgaaattc 
      caattcaag aatttccaa ctctcgggc gttgtttat 
      ttatttaat tacgtcttt ttgcaacac caaacttgg 
      gtctataag gacgagaat caaaagacg caaatcaca 
      ctcgagtgc cttaaaatt tttcattac gttttgcaa 
      aaacaggtc gaggatttg accgacatt gagctgaaa 
      atcttccag ttgtgcaat cacgctttt ttgcacaac 
      gtctacgta atcgactcg ttcctgcgc ttgtttgtt 
      aaggagaga tattatttg atttacgca atggaacgg 
      gaatcgaat tggcatcag ggcccgtct ttagacacc 
      ttacaatgt atcaggatg cagttagct cttctcctg 
      ttaattatg aatcggaaa aaagcaatt ggtgattgt 
      tgcttcagt gagagactg cccttcgtc gtaaaacca 
      gtagcttcg tattttacc aacgcgtat agcccgctt 
      attgccgag ctgtcctgc cgttttttg gaaaaatta 
      tcacaagat ccgtttgtt cccttactt gcgttactt 
      gagctggac cattatcaa gtccagtcg ctgtttttg 
      gaacggtta agggaccct ctggaggag gaaaacgtg 
      aaaatggca atcatcgac ctaattaac acgtgtatt 
      tcaagccag gacggcatg actgcggcc ttcttcaac 
      ttgaagtgt ttcatgtac tgggacggg gccgagaac 
      gatgttatt aacggcgac agcgtgagc gactttatg 
      gtcgattac ttgcaaaat attaaaaag tcgcacgaa 
      tatttcaaa aatccgctc cagctcgga atcctccga 
      ctcctcttc aacttgtgg ctaaaccac cgggaaaat 
      ttaatcgaa aacatcgca agcttgaag gatttctgg 
      cccgtgatg gcggacccg tttttctgc gactataag 
      caagacatt gaaatttac acaatcatc ctccgcata 
      attaacttg gaaatcggg gctaacata gacaaggtg 
      gacgaaaaa ctcgtaaaa accatcgat aagtttttg 
      aaggacaag aaacgcatt gcacagtgg aacaaattc 
      gtgcttgcg tcctctcaa agcagtgcg aaaaacaag 
      ctggacttg ctcagtgcc tggatggag tttttggtt 
      ttgacgaag aaagttata cctgatagt ttcgagaac 
      gacgttaag ttcgccctc gttgcctgt tgtctggac 
      ggcttgcac cctcctgaa ggcgggttc agtaacatc 
      gaaacgatt tatctgtgg tcacagctg tacttgctg 
      ttaataagt acgtggtca gtctacgag aaaaaggag 
      caagtggtt ttggggaag ctacaaacg ttcctgcac 
      gcgcttaac acgtattat aaatatctg actccgaaa 
      atcaaggag aatatttta tgtgcggtg aatcggact 
      atcctcgat ctgcaggag tacttcagt gttaataca 
      gcgcagttg ttgacattt ttgtactcc gttgggccg 
      ctgattgac gcggaatat tactacctg gtgtccgaa 
      gcgttcgag actgaggac gatgcggaa aaagtgcgg 
      cgacttaag ccgtggttg attattgtt tttatcgga 
      aactcgata atggcgctc gatcgctgc gaggaaatt 
      gggttgtgg ttctactac gagcagttt ttgcaacgg 
      gtgatggac tctgtgggg tcaatgatg gaacatagc 
      gccactttg ccgtttgca aagctagct attgacttt 
      cttatcact tatgcggag tctccgttc gttaaggac 
      ttcctaaag gaggatctg ttcagtttc tactttaaa 
      gtgcgaccg ccaccgctc acaataagt gtgggggag 
      gcaattcta tcgggactt ccggtcaat ttgaaggag 
      tggtggttt attttaatc actttgata aagctgaac 
      cgtattttg atacaacag atcggcgat gcaatgcta 
      aggacgtgc ttcacgttc atcacacaa cacgacttg 
      ttgctcaag gaaattgtt tctttgacc aaatacacc 
      gttgatatg tcggccttg actctcgtt tgtgaaact 
      ttgaaactt attctggtt gttctgaca caaatgcca 
      cattggcga gtcgactcg ccgtactct tacaactta 
      ataatggag ggtataaaa acgacaata cacgcctgc 
      gttttgtca atcctgggg tacaaaaaa attaatttt 
      tatgacatc atcaacgga aaaaatctg aacattatt 
      gacgtcacc ccaaccgac ttactagtc tcagtgttg 
      aataaacta acagaaatt acgtactgg ggctgcagc 
      tgtttgaag aaaaccaag accgactta gtaacccag 
      ctaaacgcc cactacgag actgaaaac ctcgcatta 
      ttattggtc gaaaacgac tttagtgtt ccccgattt 
      gagttgccg atcactccg agactgacg tacgggtct 
      ctattgtgc ctggctgac tatttgtgc aaaaccctc 
      aatcagttg tctcctgca actccttcg agaatttcg 
      agaagtcgt caagtgtct ctaaccgat cttaacaat 
      caaatgaaa gtcaagagt ttggggaaa attttcgag 
      cattgcacc gcaacgtat gaggaattt ataggtttt 
      atggtctac atccaaaaa agcgacgat cttccgaat 
      gaaaagttc agttcgttt ttgcactac gacatgacg 
      gaattttcc gggacgact tcggcggtt tcccagttg 
      gattatcat ttggttaag aacgcgcta gaggcgttg 
      atgagtttc ctggctggg gaaatcttc ttcgcaatt 
      cggaatctc cgaggcgaa gctgtattt gcatacagg 
      agggagttg agttcggaa atacagttc ttctacgag 
      tttgtccga aagcggact agtgaacaa tacagtgcg 
      atggtggca acacctcga tcaacgtcc tccatcgca 
      cagactgag cccgacctt atcaaaaga tacatcatc 
      accaaagtc atggataac gataccagc gatattgtt 
      gataataat tttgtactt gttatttct cagtggtta 
      atcaaattc tgccacttg tcaaattaa
    mRNA sequence
    • >TC004742 RA
      taaaagtgg cggtctata tctacataa cctgcaaac 
      ttttgcttg tgcttgtgt gtgtatttg taatgttaa 
      tttcccaat taaaaaaat gtcttcttc caccccttt 
      ttggagaaa aatccacca aacaatatc gggccatag 
      ccaaaacat tactgaaga catagtcgc ggaaatttt 
      aaaactctc gaaaacaga attacttga aggcctctt 
      gagctatcg accgtacac aaaaaaggg ttatcatga 
      ctgggttgc ggtctcgga cgttagcat ttgcatgaa 
      agaattcgt tcatgatat aagccaagt tcttaatct 
      ggacacaat aacggctta tccggtcat gtgcaatta 
      cctgatgtt tgagtatta tggaaaaat taaggagtt 
      ttggcaaat aactagaat gggttcaac cttacattt 
      gaaagagag tatttggaa tttttacac ggctgaaag 
      gatgttttt gctgaagac gtggagaca catatttga 
      gtgtgtcaa taaggatta tgagtttgc tgagcagta 
      caaggattt tctaaaaac gatcaaaat cgaggaatt 
      gcaaaataa cttgattac gcagtttga ggagctcct 
      taacgaggt cacgtctaa taattttga tgagaggag 
      tccgtacaa tgaaaactg gctgtgtcg gaacatcag 
      ggaacaaat agaaattct cctgattct catcctaac 
      agccaccca taaacccct ggcaactgc aaaactctc 
      tcatttgat taagttatt cactcagaa taatttcgg 
      cacccagcc ggcattttt cgacatccg gacgtacgc 
      aaaaccgca agacttgga ccagttaat gtttgccga 
      agtcgggtg ttactttgc cattttgga caattattg 
      ggataagga aattccaga tttttggaa acaagactc 
      aacaagcga gttggacaa gtcgctgct taacttaca 
      cctcaaccc agggcacat tataataat gctggcctg 
      gttaactct gaaatttaa atcgctaaa tcgtgacca 
      agacgccga aaaaatact agagctgtt caaaagaca 
      gccgtttat tttgtggca caaaatcct ctcagatag 
      tatgtttaa gaactgtac ggttggaga agtcgtttt 
      gcgagcaac gcataaaat gctcgacga gctttgttt 
      gatgttcga cgaccgaaa aattctcta cgaggacgg 
      cggcgtgat taaaatcct tgccgagtt tttgaaaca 
      accagactt ggccgaaat ttgtttgca aacgcgaaa 
      tggtttaga ctgcatgac ggaaatcgc gattgagtc 
      atttcctca cgattttta ttcgttcac caccattgc 
      acaatcgct gctaggcct gcgtgacca atacaaaca 
      aaccatgca attgttgaa taatttccc gtcgttttt 
      gtccgaaat gacctggac cattccaaa ggaacaaat 
      ctcggtttt gcgcaaaaa ccagccggc gtttcccga 
      cagcgacct gatagtctt cccgccgaa aacggtggt 
      gcggtcgta ccgaatttt cggaaaaaa cctgataca 
      gtacaaggg gaagtacag ctacttctt ccacctcga 
      gtatttggt cacaacgct gataaacaa cgggctgtg 
      ccaccgcaa gtttgacga ggctttggt ccagaaggt 
      cattgccgg gtatggcgt tgtgatcca agctattag 
      gtattgcgt ggacatgga cttgagaac agtggggct 
      tgagagaat tattctgaa attaaacaa actcctagt 
      gcatttgga cggcccttt gctgcactt tgagttggt 
      caaacttta cttcgactt gcaaaatgc gctaatgct 
      gtaccgaca aagcagctt cttcaagat ctgctccga 
      aatcgtatg gaaaatatt tttcccgaa gctcaaacc 
      ggaatattt gacccaaga cgttaaaat tttgctgct 
      gaatcagta cgtgtttga ggaccgaat tttcatgca 
      attgttcca aaaagagga gtttaagga agatcacac 
      tctccttct tacctacat caagattct aagcaacat 
      tattaaggt gaaattcca attcaagaa tttccaact 
      ctcgggcgt tgtttattt atttaatta cgtcttttt 
      gcaacacca aacttgggt ctataagga cgagaatca 
      aaagacgca aatcacact cgagtgcct taaaatttt 
      tcattacgt tttgcaaaa acaggtcga ggatttgac 
      cgacattga gctgaaaat cttccagtt gtgcaatca 
      cgctttttt gcacaacgt ctacgtaat cgactcgtt 
      cctgcgctt gtttgttaa ggagagata ttatttgat 
      ttacgcaat ggaacggga atcgaattg gcatcaggg 
      cccgtcttt agacacctt acaatgtat caggatgca 
      gttagctct tctcctgtt aattatgaa tcggaaaaa 
      agcaattgg tgattgttg cttcagtga gagactgcc 
      cttcgtcgt aaaaccagt agcttcgta ttttaccaa 
      cgcgtatag cccgcttat tgccgagct gtcctgccg 
      ttttttgga aaaattatc acaagatcc gtttgttcc 
      cttacttgc gttacttga gctggacca ttatcaagt 
      ccagtcgct gtttttgga acggttaag ggaccctct 
      ggaggagga aaacgtgaa aatggcaat catcgacct 
      aattaacac gtgtatttc aagccagga cggcatgac 
      tgcggcctt cttcaactt gaagtgttt catgtactg 
      ggacggggc cgagaacga tgttattaa cggcgacag 
      cgtgagcga ctttatggt cgattactt gcaaaatat 
      taaaaagtc gcacgaata tttcaaaaa tccgctcca 
      gctcggaat cctccgact cctcttcaa cttgtggct 
      aaaccaccg ggaaaattt aatcgaaaa catcgcaag 
      cttgaagga tttctggcc cgtgatggc ggacccgtt 
      tttctgcga ctataagca agacattga aatttacac 
      aatcatcct ccgcataat taacttgga aatcggggc 
      taacataga caaggtgga cgaaaaact cgtaaaaac 
      catcgataa gtttttgaa ggacaagaa acgcattgc 
      acagtggaa caaattcgt gcttgcgtc ctctcaaag 
      cagtgcgaa aaacaagct ggacttgct cagtgcctg 
      gatggagtt tttggtttt gacgaagaa agttatacc 
      tgatagttt cgagaacga cgttaagtt cgccctcgt 
      tgcctgttg tctggacgg cttgcaccc tcctgaagg 
      cgggttcag taacatcga aacgattta tctgtggtc 
      acagctgta cttgctgtt aataagtac gtggtcagt 
      ctacgagaa aaaggagca agtggtttt ggggaagct 
      acaaacgtt cctgcacgc gcttaacac gtattataa 
      atatctgac tccgaaaat caaggagaa tattttatg 
      tgcggtgaa tcggactat cctcgatct gcaggagta 
      cttcagtgt taatacagc gcagttgtt gacattttt 
      gtactccgt tgggccgct gattgacgc ggaatatta 
      ctacctggt gtccgaagc gttcgagac tgaggacga 
      tgcggaaaa agtgcggcg acttaagcc gtggttgat 
      tattgtttt tatcggaaa ctcgataat ggcgctcga 
      tcgctgcga ggaaattgg gttgtggtt ctactacga 
      gcagttttt gcaacgggt gatggactc tgtggggtc 
      aatgatgga acatagcgc cactttgcc gtttgcaaa 
      gctagctat tgactttct tatcactta tgcggagtc 
      tccgttcgt taaggactt cctaaagga ggatctgtt 
      cagtttcta ctttaaagt gcgaccgcc accgctcac 
      aataagtgt gggggaggc aattctatc gggacttcc 
      ggtcaattt gaaggagtg gtggtttat tttaatcac 
      tttgataaa gctgaaccg tattttgat acaacagat 
      cggcgatgc aatgctaag gacgtgctt cacgttcat 
      cacacaaca cgacttgtt gctcaagga aattgtttc 
      tttgaccaa atacaccgt tgatatgtc ggccttgac 
      tctcgtttg tgaaacttt gaaacttat tctggttgt 
      tctgacaca aatgccaca ttggcgagt cgactcgcc 
      gtactctta caacttaat aatggaggg tataaaaac 
      gacaataca cgcctgcgt tttgtcaat cctggggta 
      caaaaaaat taattttta tgacatcat caacggaaa 
      aaatctgaa cattattga cgtcacccc aaccgactt 
      actagtctc agtgttgaa taaactaac agaaattac 
      gtactgggg ctgcagctg tttgaagaa aaccaagac 
      cgacttagt aacccagct aaacgccca ctacgagac 
      tgaaaacct cgcattatt attggtcga aaacgactt 
      tagtgttcc ccgatttga gttgccgat cactccgag 
      actgacgta cgggtctct attgtgcct ggctgacta 
      tttgtgcaa aaccctcaa tcagttgtc tcctgcaac 
      tccttcgag aatttcgag aagtcgtca agtgtctct 
      aaccgatct taacaatca aatgaaagt caagagttt 
      ggggaaaat tttcgagca ttgcaccgc aacgtatga 
      ggaatttat aggttttat ggtctacat ccaaaaaag 
      cgacgatct tccgaatga aaagttcag ttcgttttt 
      gcactacga catgacgga attttccgg gacgacttc 
      ggcggtttc ccagttgga ttatcattt ggttaagaa 
      cgcgctaga ggcgttgat gagtttcct ggctgggga 
      aatcttctt cgcaattcg gaatctccg aggcgaagc 
      tgtatttgc atacaggag ggagttgag ttcggaaat 
      acagttctt ctacgagtt tgtccgaaa gcggactag 
      tgaacaata cagtgcgat ggtggcaac acctcgatc 
      aacgtcctc catcgcaca gactgagcc cgaccttat 
      caaaagata catcatcac caaagtcat ggataacga 
      taccagcga tattgttga taataattt tgtacttgt 
      tatttctca gtggttaat caaattctg ccacttgtc 
      aaattaaat tgtatttcg tgtatcagg tttcaaata 
      aaaaatatt ttataaaaa tacatttat ttcaccaca 
      gactcaaca attttacgg aagtaacac aaaggcgta 
      gctgtgttg agctccgcc catttcaac taattcgaa 
      acatatttt cgttaccaa ttttgtcgc agc
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