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Gene information
General information
TC004716 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 4259039..4262319
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004716
    iBeetle screen iB_00758
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004716 RA
      MTNVTQNIV IKYPREATI FASVCAILF CIVGVVGNL 
      VTVVALIRC PKLRTHATT AFVLSLCIS DLLFCSVNL 
      PLTASRYIH EAWTLGDAL CKLFPVLFY GNVAVSLLN 
      MVAITLNRY VLISYHQYY NQLYSKASI WLQLLCTWG 
      FAFLIMLPS LLGVWGQLG LNPSTFSCT ILEKDGKSP 
      KKILFLIAF VLPCVVIIS SYSCIFWRV QKSRRKLKA 
      HQQATKKEK RSSRRERDD SRLTKLMLI IFICFVLCF 
      LPLMLVNVF DDDVKYPVL HVLASILAW ASSVINPFI 
      YAASNRQYR SAYSKLFRI VKSSVVFSE SKQFSNNSL 
      KSRDKNGQS VNYKPNPGS S
    CDS sequence
    • >TC004716 RA
      atgactaat gttactcaa aatattgta ataaaatac 
      cctcgcgaa gccaccatt ttcgcgtcg gtttgtgcc 
      attttattt tgtatagta ggagttgtc ggaaatttg 
      gtgacagtc gtggcgcta atccggtgc ccgaaattg 
      cgaacgcat gccacaact gcgtttgtt ttgtcattg 
      tgcatatcc gatcttttg ttttgttcg gtgaatctt 
      cccctgacc gcctccagg tatatccac gaggcctgg 
      acgctcggg gatgctctc tgcaaattg tttccggtc 
      ttgttttac ggaaacgtg gccgtttct cttctcaat 
      atggtcgcg attacattg aacaggtac gttctgatc 
      tcgtaccat caatattac aatcaattg tattcgaaa 
      gcctccata tggttgcag ctgctttgt acttggggt 
      ttcgctttc ttaataatg ttgccgtca cttttggga 
      gtttgggga cagctgggt cttaatcct tcgaccttt 
      tcttgcacg attttggaa aaagacggc aaatcgccg 
      aagaagatt ctttttctt atagctttc gtactgcca 
      tgcgtcgtc ataatcagt tcatactcg tgtatattt 
      tggcgagtg cagaaatca cgccgaaag ttaaaggcg 
      caccagcaa gcgacaaaa aaagaaaaa cggagtagc 
      cgccgagaa cgagacgac agccgactc accaaactc 
      atgttaatc atatttatt tgtttcgta ttgtgtttt 
      ttaccgtta atgttggtt aatgttttt gatgacgac 
      gtcaagtat ccagttttg cacgtattg gcatctatt 
      ttagcatgg gcttccagt gtcataaat ccatttata 
      tatgcggct agcaatcga cagtataga tcagcatat 
      tcaaaactg tttagaatt gttaaatcc agtgtcgtt 
      ttctcggag tctaagcag ttttcgaat aattcacta 
      aagtcgagg gacaaaaac ggacaatcg gttaactac 
      aaacccaat cctggatcg tcctag
    mRNA sequence
    • >TC004716 RA
      caactttta aaaacagtg cgcgtccga tagccgagc 
      ggttatgtg acatgttca tcagaaaat cgtcgtgca 
      tttatttaa taaaacctc aatggaaaa actgtcaaa 
      atcatgact aatgttact caaaatatt gtaataaaa 
      taccctcgc gaagccacc attttcgcg tcggtttgt 
      gccatttta ttttgtata gtaggagtt gtcggaaat 
      ttggtgaca gtcgtggcg ctaatccgg tgcccgaaa 
      ttgcgaacg catgccaca actgcgttt gttttgtca 
      ttgtgcata tccgatctt ttgttttgt tcggtgaat 
      cttcccctg accgcctcc aggtatatc cacgaggcc 
      tggacgctc ggggatgct ctctgcaaa ttgtttccg 
      gtcttgttt tacggaaac gtggccgtt tctcttctc 
      aatatggtc gcgattaca ttgaacagg tacgttctg 
      atctcgtac catcaatat tacaatcaa ttgtattcg 
      aaagcctcc atatggttg cagctgctt tgtacttgg 
      ggtttcgct ttcttaata atgttgccg tcacttttg 
      ggagtttgg ggacagctg ggtcttaat ccttcgacc 
      ttttcttgc acgattttg gaaaaagac ggcaaatcg 
      ccgaagaag attcttttt cttatagct ttcgtactg 
      ccatgcgtc gtcataatc agttcatac tcgtgtata 
      ttttggcga gtgcagaaa tcacgccga aagttaaag 
      gcgcaccag caagcgaca aaaaaagaa aaacggagt 
      agccgccga gaacgagac gacagccga ctcaccaaa 
      ctcatgtta atcatattt atttgtttc gtattgtgt 
      tttttaccg ttaatgttg gttaatgtt tttgatgac 
      gacgtcaag tatccagtt ttgcacgta ttggcatct 
      attttagca tgggcttcc agtgtcata aatccattt 
      atatatgcg gctagcaat cgacagtat agatcagca 
      tattcaaaa ctgtttaga attgttaaa tccagtgtc 
      gttttctcg gagtctaag cagttttcg aataattca 
      ctaaagtcg agggacaaa aacggacaa tcggttaac 
      tacaaaccc aatcctgga tcgtcctag atccctcct 
      gtccacctc ttgccactc agtcattcc ttatcaaat 
      ggaacattt gccaaatat ttgaaagac tgacatgat 
      aagaaattg ctatatttc ccatgcaca agagttaat 
      acaatttgt cgcaaaagc agtattact atttccaaa 
      tgatacaca cacagtgat atctactaa acaaacttt 
      tgtgatatt ctagatacc gtaagtaac taaaattta 
      tacctttaa gatattatt attttatat ttattgttc 
      gagaccctt ttctttgta actgagcat ttaagtttt 
      gactgaatt tttaagttc aactgtaca tatgtataa 
      tgtatacaa acaagagaa ataaatttt ttaaaaccc 
      a