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Gene information
General information
TC004712 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 863158..868269
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004712
    iBeetle screen iB_00756

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004712 RA
      MAMYKGFSV LEKSKPPNP HSVLLQRRG KDETLLFES 
      QAVAVLSTP EMEVVKKEY TKILDAYGC LGVLQLYSG 
      DSTVLYLVM VTGCFSVGK IGDSEIFRI TQTQFVPLH 
      YQQNEDRIA EVRKLLNSG TFYFSWYSG TPGGSQLDL 
      TLCAQRRHK TITTDHRFF WNRMLHVHL VRFGVDCNS 
      WLVRAMCGS VEVRTVYVG HRKALAAVV SRLSCERAG 
      TRFNVRGSN DEGHVANFV ETEQVIYLE NEVSSYLQT 
      RGSVPLFWE QPGVQVGSH KVRISRGYE ASKAAFDRH 
      MKTIKERYG KQVIVNLLG TSLIGSKEG EATLSQEFQ 
      KHHKESCHT DIPHIVFDY HQECRGGNQ ANLQKLKAK 
      VDEQLQEFS FYHAIGSTI YSLQTGTIR TNCLDCLDR 
      TNCVQTFFG LEILGRQLQ AMQLVDKKQ TVSRFEEVF 
      KQMWINNGN EISKIYAGT GAIQGGSKL MDGARSAAR 
      TIQNNLLDN SKQEAIDIL LVGSTLSTE LADRARTLL 
      PYNTLHAPP HVLREMCKR YTEYTDPLP IRIAVGTYN 
      VNGGKHFRS VVFKDIKLS DWLLDPHIK NPGALVDTG 
      YTDENKSIP VDIYAIGFE EIVDLNAAN IVNTSSENA 
      KSWATELQK VLSRDRPYV LVTYQQLVG VCLYVFVRP 
      EHVPYIRDV AVDSVKTGL GGHTGNKGA AAIRLVFHA 
      TSLCFVCAH FAAGQSQVS ERNADYNEI TRKITFPMS 
      RSLNSHEYL FWCGDFNYR VDMDKDEIK ESVKLGDYD 
      RILENDQLM KQQQEGNVF KDFTEGKIT FPPTYKYDL 
      FSDDYDTSE KCRAPAWTD RVLWRRRKQ SLAITSQLE 
      DSNPGKLVF YGRAELKQS DHRPVIAII DITARKIDE 
      TKRQNMFYE VIADMGPPD GTIIVHYED SSQNDEVFD 
      DQLVATLLD DFTQFGEAI LVRFIADTM WITFRDGQS 
      VLKIMNKTQ GRIPEYNLT LSLKTPNWV EQAKEEVGL 
      CSSNTVPLY TSPTQSDYN CLGIPEVKA SRSGAPPSR 
      PPPPSVKSP ATPKRPPPR VGVISLPTA NSSAPPSMP 
      PPELPEDTG IYEEINDDI VSAPEPQGP PPPPPRPEP 
      APPTPQREP PPVPARSAP PPPLPARPR Q
    CDS sequence
    • >TC004712 RA
      atggctatg tataagggc ttttccgtt cttgaaaaa 
      tctaagcct cctaatccc catagtgtc ctattgcaa 
      cgaaggggc aaagatgag actttactt ttcgaatcc 
      caagctgtg gctgtcctt tccacaccg gaaatggaa 
      gtcgtcaaa aaggagtac accaaaata ttagatgct 
      tatggctgt ttaggggtc ctccagctc tattccgga 
      gactcgaca gtcctatac cttgtcatg gtcactggg 
      tgtttctca gtggggaaa atcggggat tcggagatt 
      tttagaata acacaaacg cagtttgtt cctttgcac 
      taccaacaa aacgaggat agaatagcc gaagtgcgc 
      aaattgctc aattctgga actttttac ttctcatgg 
      tactctggg acgcccggt ggtagtcaa ctggacttg 
      actttgtgt gcgcaaagg cggcataaa actatcaca 
      actgatcat agatttttc tggaaccgg atgttacac 
      gttcactta gttagattc ggggttgat tgtaattcg 
      tggctggtg agggccatg tgtggatcg gttgaagtc 
      aggacggtc tacgtcgga cacaggaag gctttagct 
      gctgttgtt tcgcgccta agctgtgaa agagccggg 
      acgagattc aatgtgaga gggagtaat gatgaaggc 
      cacgttgcg aattttgtc gaaacggag caagttatt 
      tatttagaa aatgaagtg tcttcgtat ttgcagact 
      agaggttcg gtaccgctt ttttgggaa caaccggga 
      gtacaagta ggttcgcat aaagttcgg atttcgcgc 
      gggtatgaa gcctcaaag gctgcgttt gatcgccac 
      atgaagacc atcaaagag agatacggg aaacaggtc 
      attgtcaat cttttgggc accagtttg attggtagc 
      aaggaaggc gaagctacc ttaagtcag gaatttcaa 
      aaacaccac aaagaatca tgtcatacc gacattccg 
      catatcgtg tttgactac caccaggaa tgccgcggt 
      gggaaccaa gcaaattta caaaaactt aaagccaaa 
      gtcgacgaa caattacaa gaattttcg ttctaccac 
      gcaattggg agtacaata tacagctta caaacgggc 
      acaatccga acaaattgt ttagactgt ttagatagg 
      actaattgt gttcaaacg tttttcggg ttagagatt 
      ttaggcaga caactgcag gcgatgcag ttagttgat 
      aagaagcaa accgtttcg cggtttgag gaggttttc 
      aagcaaatg tggatcaat aacgggaac gaaattagt 
      aagatttac gccgggact ggcgccata caaggcgga 
      tcaaagcta atggatggg gcccgatcc gcagctaga 
      acaatccag aacaacctc ctcgacaac tccaaacaa 
      gaagcgatc gacattttg ctcgtgggc tccactcta 
      tcgacggaa cttgccgat cgggcacga accctcctc 
      ccgtataac accctccac gcgccaccc cacgtcctg 
      cgggaaatg tgcaaacga tacaccgag tataccgac 
      cctctccct atacgtata gccgtcggg acatacaat 
      gtcaacggc ggcaaacac ttccgcagt gtcgtgttc 
      aaagatatc aaactatct gattggtta ctggaccct 
      catataaaa aatcccgga gcgctagtc gatacggga 
      tacaccgac gaaaataaa tcaattcct gtcgatatc 
      tacgcgatt ggttttgag gaaattgtc gatttgaac 
      gccgccaat atcgttaac accagcagc gaaaacgcg 
      aagtcttgg gcgacggag ttgcagaag gtgttgtcg 
      cgggatcgc ccttacgtc ttggtcaca tatcagcaa 
      ttagttggg gtgtgcttg tacgttttt gtaaggcct 
      gaacatgtg ccttacatc cgcgacgtt gcggtcgac 
      tcggttaaa accggtcta ggcgggcat acgggcaat 
      aagggggcc gctgccatc agactggtg tttcacgca 
      acgtctttg tgcttcgtt tgtgcacat tttgcagcc 
      ggacagtcg caagttagc gagagaaat gccgattat 
      aatgaaatt acgcggaaa attacgttc ccgatgagc 
      cggtctttg aattcgcac gagtattta ttttggtgt 
      ggcgacttt aactacaga gttgacatg gataaagat 
      gaaataaag gaatcagta aagttgggt gactatgac 
      cggattcta gagaatgac caactgatg aaacaacaa 
      caagaagga aacgtgttt aaggatttt actgaagga 
      aaaattacg tttccgcct acgtacaag tatgattta 
      tttagcgac gattatgac actagtgaa aagtgtcga 
      gcaccagcg tggacagac cgtgttctt tggcggaga 
      cgcaagcaa tctttagct attacaagt caattagaa 
      gattcaaat cctggtaaa ttagtgttc tatggccgc 
      gccgaattg aaacaaagt gaccataga ccggttatc 
      gcaattatc gatataacc gctcgaaaa attgatgaa 
      acgaagcga caaaatatg ttttacgaa gtaatagct 
      gacatgggg cctcctgat ggtactata atagtccat 
      tatgaggac tcgtctcaa aacgatgaa gttttcgac 
      gaccaactt gtcgcaaca cttctggac gacttcact 
      caatttggt gaagcgatt ttggtgcga ttcatcgcc 
      gataccatg tggatcact ttccgggac ggccaatcc 
      gtactcaaa attatgaac aagacgcaa ggacggatt 
      cccgaatat aacttaacc ctatcattg aaaacccct 
      aactgggtg gagcaagcg aaggaggag gttggtctg 
      tgctccagc aacaccgtc cccttgtac acctccccc 
      acccagtcc gactacaac tgtttaggc attccagaa 
      gtgaaggcg tctcggtcg ggggcgcct cccagccgc 
      cccccgccg ccgtcggtg aagtccccg gcgacgccc 
      aagcggccg ccccccaga gtgggggtg ataagcctc 
      ccgacggcg aattcatcc gcaccccct agtatgccg 
      ccccctgaa ttgccagag gacaccggt atttacgaa 
      gagatcaac gacgatatt gtgagcgcg cctgagccg 
      caagggccg ccgccgccg cccccacgg ccggaaccg 
      gcgcctcct acgccacaa agagaaccg ccccctgta 
      cctgcccgg tccgcaccg ccccctcct cttcctgcg 
      cggccacgt caataa
    mRNA sequence
    • >TC004712 RA
      tacggcgac gcaagtgga attgatttt ggttaggat 
      tagggggtg tatgtgctc gaatatcaa cagatcagc 
      tgtagtcag tgtaaaatc gccaataaa atgatcgaa 
      taagcgaat ctccaccat ggctatgta taagggctt 
      ttccgttct tgaaaaatc taagcctcc taatcccca 
      tagtgtcct attgcaacg aaggggcaa agatgagac 
      tttactttt cgaatccca agctgtggc tgtcctttc 
      cacaccgga aatggaagt cgtcaaaaa ggagtacac 
      caaaatatt agatgctta tggctgttt aggggtcct 
      ccagctcta ttccggaga ctcgacagt cctatacct 
      tgtcatggt cactgggtg tttctcagt ggggaaaat 
      cggggattc ggagatttt tagaataac acaaacgca 
      gtttgttcc tttgcacta ccaacaaaa cgaggatag 
      aatagccga agtgcgcaa attgctcaa ttctggaac 
      tttttactt ctcatggta ctctgggac gcccggtgg 
      tagtcaact ggacttgac tttgtgtgc gcaaaggcg 
      gcataaaac tatcacaac tgatcatag atttttctg 
      gaaccggat gttacacgt tcacttagt tagattcgg 
      ggttgattg taattcgtg gctggtgag ggccatgtg 
      tggatcggt tgaagtcag gacggtcta cgtcggaca 
      caggaaggc tttagctgc tgttgtttc gcgcctaag 
      ctgtgaaag agccgggac gagattcaa tgtgagagg 
      gagtaatga tgaaggcca cgttgcgaa ttttgtcga 
      aacggagca agttattta tttagaaaa tgaagtgtc 
      ttcgtattt gcagactag aggttcggt accgctttt 
      ttgggaaca accgggagt acaagtagg ttcgcataa 
      agttcggat ttcgcgcgg gtatgaagc ctcaaaggc 
      tgcgtttga tcgccacat gaagaccat caaagagag 
      atacgggaa acaggtcat tgtcaatct tttgggcac 
      cagtttgat tggtagcaa ggaaggcga agctacctt 
      aagtcagga atttcaaaa acaccacaa agaatcatg 
      tcataccga cattccgca tatcgtgtt tgactacca 
      ccaggaatg ccgcggtgg gaaccaagc aaatttaca 
      aaaacttaa agccaaagt cgacgaaca attacaaga 
      attttcgtt ctaccacgc aattgggag tacaatata 
      cagcttaca aacgggcac aatccgaac aaattgttt 
      agactgttt agataggac taattgtgt tcaaacgtt 
      tttcgggtt agagatttt aggcagaca actgcaggc 
      gatgcagtt agttgataa gaagcaaac cgtttcgcg 
      gtttgagga ggttttcaa gcaaatgtg gatcaataa 
      cgggaacga aattagtaa gatttacgc cgggactgg 
      cgccataca aggcggatc aaagctaat ggatggggc 
      ccgatccgc agctagaac aatccagaa caacctcct 
      cgacaactc caaacaaga agcgatcga cattttgct 
      cgtgggctc cactctatc gacggaact tgccgatcg 
      ggcacgaac cctcctccc gtataacac cctccacgc 
      gccacccca cgtcctgcg ggaaatgtg caaacgata 
      caccgagta taccgaccc tctccctat acgtatagc 
      cgtcgggac atacaatgt caacggcgg caaacactt 
      ccgcagtgt cgtgttcaa agatatcaa actatctga 
      ttggttact ggaccctca tataaaaaa tcccggagc 
      gctagtcga tacgggata caccgacga aaataaatc 
      aattcctgt cgatatcta cgcgattgg ttttgagga 
      aattgtcga tttgaacgc cgccaatat cgttaacac 
      cagcagcga aaacgcgaa gtcttgggc gacggagtt 
      gcagaaggt gttgtcgcg ggatcgccc ttacgtctt 
      ggtcacata tcagcaatt agttggggt gtgcttgta 
      cgtttttgt aaggcctga acatgtgcc ttacatccg 
      cgacgttgc ggtcgactc ggttaaaac cggtctagg 
      cgggcatac gggcaataa gggggccgc tgccatcag 
      actggtgtt tcacgcaac gtctttgtg cttcgtttg 
      tgcacattt tgcagccgg acagtcgca agttagcga 
      gagaaatgc cgattataa tgaaattac gcggaaaat 
      tacgttccc gatgagccg gtctttgaa ttcgcacga 
      gtatttatt ttggtgtgg cgactttaa ctacagagt 
      tgacatgga taaagatga aataaagga atcagtaaa 
      gttgggtga ctatgaccg gattctaga gaatgacca 
      actgatgaa acaacaaca agaaggaaa cgtgtttaa 
      ggattttac tgaaggaaa aattacgtt tccgcctac 
      gtacaagta tgatttatt tagcgacga ttatgacac 
      tagtgaaaa gtgtcgagc accagcgtg gacagaccg 
      tgttctttg gcggagacg caagcaatc tttagctat 
      tacaagtca attagaaga ttcaaatcc tggtaaatt 
      agtgttcta tggccgcgc cgaattgaa acaaagtga 
      ccatagacc ggttatcgc aattatcga tataaccgc 
      tcgaaaaat tgatgaaac gaagcgaca aaatatgtt 
      ttacgaagt aatagctga catggggcc tcctgatgg 
      tactataat agtccatta tgaggactc gtctcaaaa 
      cgatgaagt tttcgacga ccaacttgt cgcaacact 
      tctggacga cttcactca atttggtga agcgatttt 
      ggtgcgatt catcgccga taccatgtg gatcacttt 
      ccgggacgg ccaatccgt actcaaaat tatgaacaa 
      gacgcaagg acggattcc cgaatataa cttaaccct 
      atcattgaa aacccctaa ctgggtgga gcaagcgaa 
      ggaggaggt tggtctgtg ctccagcaa caccgtccc 
      cttgtacac ctcccccac ccagtccga ctacaactg 
      tttaggcat tccagaagt gaaggcgtc tcggtcggg 
      ggcgcctcc cagccgccc cccgccgcc gtcggtgaa 
      gtccccggc gacgcccaa gcggccgcc ccccagagt 
      gggggtgat aagcctccc gacggcgaa ttcatccgc 
      accccctag tatgccgcc ccctgaatt gccagagga 
      caccggtat ttacgaaga gatcaacga cgatattgt 
      gagcgcgcc tgagccgca agggccgcc gccgccgcc 
      cccacggcc ggaaccggc gcctcctac gccacaaag 
      agaaccgcc ccctgtacc tgcccggtc cgcaccgcc 
      ccctcctct tcctgcgcg gccacgtca ataagtttt 
      cctagtcag atcaaagtt tgcgtttcc gtggccgcg 
      aggtaaaat tctggtttc ttttcttgc cttagactg 
      tgactgtag cttggctgt tattagatg ttattagtt 
      ttcttgctt cggatttgt ttagttcct attttctat 
      gaaatgact gctagggaa cattccata ttatttatt 
      attgttgat aatcaaaac gcggcgagc acaagttgt 
      aacagcaaa ttcaagaat tagtgcttt atgtgtttt 
      ggaaaacta gtttatgtt gcgtgttaa gaatttcag 
      ataattcta ttgaaattt tactgttgc ttattgttt 
      tctactaaa tagtttaaa acacataag caaataaaa 
      gatatttag aatacacag atgctgcat ttgcatcca 
      atatgctgt aaaacatgt tgatattta gaaaatttt 
      catgtaaat gtttatatc atttgttta aatttatat 
      ttagtattg ttcttttct tagtattaa ataaaccga 
      ttatcaaaa g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY