Status
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Gene information
General information
TC031464
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004698
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 46931..53209
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031464
    iBeetle screen iB_00754

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    metathorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    Pupal morphology
    oblique dorsal thoracic muscles size broader - Number of animals showing the phenotype: 8

    oblique dorsal thoracic muscles number increased - Number of animals showing the phenotype: 8
    female genital lobes rounded - Number of animals showing the phenotype: 8

    Adult morphology
    abdominal odoriferous glands partially colour darker - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection



    Fertility
    egg production: number no/almost no eggs -
    tropharium size increased - Number of animals showing the phenotype: 3

    ovariole number decreased - Number of animals showing the phenotype: 3
    mature egg number increased - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031464 RA
      MSSRQQQQN SSQQSKTAT QSYQKPYNQ ASTPQGPPP 
      PYPSPGGGS VKQFKTEVV EQKPAPQPQ THPFTLTQQ 
      QLQMLNYFQ QNINNLTPA QENMRLQLQ HQHRLMQQH 
      QQQLRHQRQ AQPPRQGST PPAYNGNNF QGDPPTLNP 
      PKNLSPPSN GDKLVQPTP NVIPSCARD ITMPVSGQN 
      SADMDVSEE ELKDFLSQK DLATTLAEN LLKHFGSDD 
      IDIKEEQEQ SDSSKPEMD TKRRIKSPP HDSILTIKS 
      EPPWEVDSL HSVEKRPEI EYTIDMDAK TILRLCKGE 
      GIKGEISNS LISDRAPPP SPPDPPAIK LNHQQLLPP 
      TPSVYLDNK KHAFSPQLQ EFCLKHPIA VVRGLASAL 
      KLDLGLFST KTLVEANPD HSVEVRTQI QQPSDENWD 
      PQSGKKVWA CISHRSHTT IARYAQYQA SSFKESLKE 
      ERDKATSGL SDSDSKDSV NFPKRRRLC NIPPLANTK 
      GCNQPKMLK FGTNVDLSD EKKWKAQLQ ELMKLPAFA 
      RVVSAGNML SHVGHVILG MNTVQLYMK VPGSRTPGH 
      QENNNFCSI NINIGPGDC EWFAVPDAY WGAICALCE 
      KNQINYLHG SWWPVLEDL YNANIPVYR FLQRPGDLV 
      WVNAGCVHW VQAVGWCNN IAWNVGPLT ARQYQLAIE 
      RYEWNKLQS FKSIVPMLH LSWNLSRNI KVSDPKLFQ 
      LIKNCLMRT LRQCSMILE FVKHKGVEV KFHGRGKNE 
      ASHYCGQCE IEVFNILFI REQEKRHVV HCMDCARKQ 
      SPNLEGFVC LEEYKMEEL TEVYDNFTL YPTVSATAE 
      QYRLM
    CDS sequence
    • >TC031464 RA
      atgtcatca aggcaacaa caacaaaac agttcgcaa 
      caatcgaaa accgccact caatcgtat caaaaaccc 
      tataatcaa gcttcgacc ccgcaaggc ccgccaccg 
      ccttacccg tccccaggc ggcggctcc gtgaaacaa 
      ttcaaaacc gaagtcgtc gaacaaaaa cccgccccc 
      caacctcaa actcacccc ttcactctc actcaacag 
      caactccaa atgttgaat tactttcaa caaaacata 
      aacaatcta acgcctgcc caagaaaat atgagactg 
      caactccaa catcagcac cggttgatg caacaacac 
      caacaacag ttgagacac caaagacaa gcccagccc 
      ccgcgacag ggctccacg ccgcctgcc tacaacgga 
      aacaacttc cagggagat cccccgact ttgaatccc 
      cccaaaaac ctttcgccc ccctccaat ggagacaaa 
      ctagtccaa ccaacacca aatgtgatc ccgagctgc 
      gcgagggac ataaccatg cccgtttcg gggcaaaat 
      tcagcagac atggacgtg agtgaggaa gaattgaag 
      gacttcctc tcgcagaag gatctagca acgacgctc 
      gccgaaaat ctgctcaag cacttcggc tccgacgac 
      atcgacatc aaagaggaa caggagcaa agtgattca 
      tcgaaaccg gaaatggac acgaaacga cgaatcaaa 
      tcgccaccg cacgatagc atcctaact atcaaatcg 
      gaaccgcct tgggaagtc gattcgctg cattctgtg 
      gaaaaacga ccggaaatc gaatacacg atcgacatg 
      gacgccaaa acaatccta agactgtgc aaaggtgag 
      ggcatcaag ggcgaaatc agcaactcg ttaatatca 
      gatcgagcg ccgccccct tcacccccc gacccccca 
      gcaataaaa ctaaaccac caacagtta cttccgccc 
      accccttcg gtctactta gataataaa aaacacgcg 
      tttagtcca cagttacag gagttttgc ctcaaacac 
      cccatagct gtagtacgc ggattagcc tcggcgtta 
      aaactcgac ttaggctta ttttcgact aaaacacta 
      gttgaggct aatcccgat cacagtgtt gaagttagg 
      acgcaaata cagcaacca tccgacgaa aactgggac 
      ccacagtcc gggaagaag gtctgggct tgtatctcg 
      cataggtcg cacacgacc attgccaga tatgcgcag 
      taccaagcc tccagcttt aaggaaagt ttaaaggaa 
      gagagggat aaggccacc agtggcttg tccgactcg 
      gattccaag gactcggtc aattttccc aagaggagg 
      cggctctgc aacatcccg cccttggcc aacaccaag 
      ggctgcaac cagcccaag atgctaaag tttgggacg 
      aacgtggac ttgtcggat gagaaaaag tggaaggcg 
      cagcttcag gagttgatg aaactgccg gcgtttgct 
      cgagttgtc tcggccgga aacatgctg tcgcacgtg 
      ggccacgtg atcctcggc atgaacacg gtccagctg 
      tacatgaag gtacctggc agtcggact ccgggccac 
      caggaaaac aacaacttt tgctcgatt aacataaac 
      atcgggcca ggtgattgc gaatggttt gcggtccct 
      gacgcatat tggggcgca atttgcgcc ctgtgcgaa 
      aagaaccaa ataaactac cttcatggg tcgtggtgg 
      cccgtcctg gaggactta tacaatgcg aatatcccc 
      gtttatcgg tttttgcaa cgtcctgga gacctggtc 
      tgggtcaat gctgggtgc gtccattgg gtgcaagct 
      gtgggctgg tgcaacaac atcgcctgg aacgtgggc 
      cctctcaca gcgcggcaa taccaactc gctatcgag 
      cgatacgag tggaataaa ctgcagagc ttcaagtca 
      attgtgcca atgttgcac ttgtcgtgg aacttgtcg 
      cggaatatc aaagtttcc gacccgaag ttgttccag 
      ttgattaag aattgctta atgcggacc ttgcgacag 
      tgttcgatg attttggag tttgtgaaa cataaaggt 
      gtcgaggtc aagttccac ggacgaggg aagaacgaa 
      gcgtcgcat tactgtggc caatgcgag atcgaggtg 
      tttaatatc ttgttcata agggagcag gagaagcgg 
      cacgtggtc cattgtatg gactgcgcg cgcaagcag 
      agcccgaac ctggagggg ttcgtgtgt ttggaggag 
      tacaaaatg gaggagttg acggaagtt tacgataac 
      ttcacttta tatcccacc gttagtgct actgctgag 
      caatatcga ctaatgtag
    mRNA sequence
    • >TC031464 RA
      aaaaaagaa aagataatg gaaaagaca aaaaagaaa 
      gtgcaatcg cggacacgt gtcggttgt caaactccc 
      taggacttg aatcaaata aactataag ctgttgata 
      gtagtgata gtatttttc gatgataag ataaggtgt 
      gaattggtc taaattatg taaaaatgt tgtaatatc 
      gaacgaact gaatttcgt gagattgtg taaacgtgg 
      gttagggga gaaaaagca ttcactagg cttgaggtg 
      agctgaacc acgtggtga atgcgtttt tctcgcgtg 
      attaattaa acaatagta aaaaatagc gagagttgt 
      tccttaagt aggcaataa ccttctagg cgtagacaa 
      ttgctctcc atagaagaa gcgtggaat cttccgata 
      tcggctgaa atgtcatca aggcaacaa caacaaaac 
      agttcgcaa caatcgaaa accgccact caatcgtat 
      caaaaaccc tataatcaa gcttcgacc ccgcaaggc 
      ccgccaccg ccttacccg tccccaggc ggcggctcc 
      gtgaaacaa ttcaaaacc gaagtcgtc gaacaaaaa 
      cccgccccc caacctcaa actcacccc ttcactctc 
      actcaacag caactccaa atgttgaat tactttcaa 
      caaaacata aacaatcta acgcctgcc caagaaaat 
      atgagactg caactccaa catcagcac cggttgatg 
      caacaacac caacaacag ttgagacac caaagacaa 
      gcccagccc ccgcgacag ggctccacg ccgcctgcc 
      tacaacgga aacaacttc cagggagat cccccgact 
      ttgaatccc cccaaaaac ctttcgccc ccctccaat 
      ggagacaaa ctagtccaa ccaacacca aatgtgatc 
      ccgagctgc gcgagggac ataaccatg cccgtttcg 
      gggcaaaat tcagcagac atggacgtg agtgaggaa 
      gaattgaag gacttcctc tcgcagaag gatctagca 
      acgacgctc gccgaaaat ctgctcaag cacttcggc 
      tccgacgac atcgacatc aaagaggaa caggagcaa 
      agtgattca tcgaaaccg gaaatggac acgaaacga 
      cgaatcaaa tcgccaccg cacgatagc atcctaact 
      atcaaatcg gaaccgcct tgggaagtc gattcgctg 
      cattctgtg gaaaaacga ccggaaatc gaatacacg 
      atcgacatg gacgccaaa acaatccta agactgtgc 
      aaaggtgag ggcatcaag ggcgaaatc agcaactcg 
      ttaatatca gatcgagcg ccgccccct tcacccccc 
      gacccccca gcaataaaa ctaaaccac caacagtta 
      cttccgccc accccttcg gtctactta gataataaa 
      aaacacgcg tttagtcca cagttacag gagttttgc 
      ctcaaacac cccatagct gtagtacgc ggattagcc 
      tcggcgtta aaactcgac ttaggctta ttttcgact 
      aaaacacta gttgaggct aatcccgat cacagtgtt 
      gaagttagg acgcaaata cagcaacca tccgacgaa 
      aactgggac ccacagtcc gggaagaag gtctgggct 
      tgtatctcg cataggtcg cacacgacc attgccaga 
      tatgcgcag taccaagcc tccagcttt aaggaaagt 
      ttaaaggaa gagagggat aaggccacc agtggcttg 
      tccgactcg gattccaag gactcggtc aattttccc 
      aagaggagg cggctctgc aacatcccg cccttggcc 
      aacaccaag ggctgcaac cagcccaag atgctaaag 
      tttgggacg aacgtggac ttgtcggat gagaaaaag 
      tggaaggcg cagcttcag gagttgatg aaactgccg 
      gcgtttgct cgagttgtc tcggccgga aacatgctg 
      tcgcacgtg ggccacgtg atcctcggc atgaacacg 
      gtccagctg tacatgaag gtacctggc agtcggact 
      ccgggccac caggaaaac aacaacttt tgctcgatt 
      aacataaac atcgggcca ggtgattgc gaatggttt 
      gcggtccct gacgcatat tggggcgca atttgcgcc 
      ctgtgcgaa aagaaccaa ataaactac cttcatggg 
      tcgtggtgg cccgtcctg gaggactta tacaatgcg 
      aatatcccc gtttatcgg tttttgcaa cgtcctgga 
      gacctggtc tgggtcaat gctgggtgc gtccattgg 
      gtgcaagct gtgggctgg tgcaacaac atcgcctgg 
      aacgtgggc cctctcaca gcgcggcaa taccaactc 
      gctatcgag cgatacgag tggaataaa ctgcagagc 
      ttcaagtca attgtgcca atgttgcac ttgtcgtgg 
      aacttgtcg cggaatatc aaagtttcc gacccgaag 
      ttgttccag ttgattaag aattgctta atgcggacc 
      ttgcgacag tgttcgatg attttggag tttgtgaaa 
      cataaaggt gtcgaggtc aagttccac ggacgaggg 
      aagaacgaa gcgtcgcat tactgtggc caatgcgag 
      atcgaggtg tttaatatc ttgttcata agggagcag 
      gagaagcgg cacgtggtc cattgtatg gactgcgcg 
      cgcaagcag agcccgaac ctggagggg ttcgtgtgt 
      ttggaggag tacaaaatg gaggagttg acggaagtt 
      tacgataac ttcacttta tatcccacc gttagtgct 
      actgctgag caatatcga ctaatgtag caggtttaa 
      tttgaagtg ctatctttc agctaccac tgccacttg 
      taacggatg tacaactta atgttaata cttgcttat 
      taaatgtaa attcgagtg ataatgtaa aatgtagat 
      aagtgtgta cataatatt ttcaatttt gtagattgc 
      cgataagtt aatgttaca tcttaatac agtgatctc 
      ataacagtt atttataag tgtacatat aaaaataaa 
      tatatttgt acgatatat ttattttgg agtcaagga 
      ataggatga ttcgaatca tcattttcg ttgttatgg 
      atgaaacca tattttcac tgccaaaaa ttatatgta 
      aatgactgt ttttacgta attttttaa gtctgtacg 
      tattgtata attcaaagt ttttttaaa ggcagcaaa 
      ttcggttat tgcgtgtat tcgttcact gccactttg 
      tgatacatt tctacccga ataccactt tatatgttc 
      atagacaga gcctgggtt cgaacagtg ccttcttca 
      ataaattat ctaaacaac attgtgaaa aaattcata 
      ttttctcca gtcatgacc aattacaaa tacagttac 
      attgtgtaa atatgatga tgtattagg aaataaatc 
      aggaaactg gctcaattt tctaagtaa ggtaatccc 
      tatttcctc tgttcatat agctgtgta ttttatatc 
      atgaaaagt ctgatttca ctttatgta ttatatagt 
      ttcaattgt agtttagat actacctaa acttgtatt 
      tatgattaa ttaaataaa acgttatat ttgga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY