General information | |||
TC031464
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 46931..53209 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC031464 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00754 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head appendages partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
metathorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 4 (16 dpi)
adult lethality - Number of animals showing the phenotype: 4 (19 dpi)
oblique dorsal thoracic muscles number increased - Number of animals showing the phenotype: 8
female genital lobes rounded - Number of animals showing the phenotype: 8
tropharium size increased - Number of animals showing the phenotype: 3
ovariole number decreased - Number of animals showing the phenotype: 3
mature egg number increased - Number of animals showing the phenotype: 3
- >TC031464 RA
MSSRQQQQN SSQQSKTAT QSYQKPYNQ ASTPQGPPP PYPSPGGGS VKQFKTEVV EQKPAPQPQ THPFTLTQQ QLQMLNYFQ QNINNLTPA QENMRLQLQ HQHRLMQQH QQQLRHQRQ AQPPRQGST PPAYNGNNF QGDPPTLNP PKNLSPPSN GDKLVQPTP NVIPSCARD ITMPVSGQN SADMDVSEE ELKDFLSQK DLATTLAEN LLKHFGSDD IDIKEEQEQ SDSSKPEMD TKRRIKSPP HDSILTIKS EPPWEVDSL HSVEKRPEI EYTIDMDAK TILRLCKGE GIKGEISNS LISDRAPPP SPPDPPAIK LNHQQLLPP TPSVYLDNK KHAFSPQLQ EFCLKHPIA VVRGLASAL KLDLGLFST KTLVEANPD HSVEVRTQI QQPSDENWD PQSGKKVWA CISHRSHTT IARYAQYQA SSFKESLKE ERDKATSGL SDSDSKDSV NFPKRRRLC NIPPLANTK GCNQPKMLK FGTNVDLSD EKKWKAQLQ ELMKLPAFA RVVSAGNML SHVGHVILG MNTVQLYMK VPGSRTPGH QENNNFCSI NINIGPGDC EWFAVPDAY WGAICALCE KNQINYLHG SWWPVLEDL YNANIPVYR FLQRPGDLV WVNAGCVHW VQAVGWCNN IAWNVGPLT ARQYQLAIE RYEWNKLQS FKSIVPMLH LSWNLSRNI KVSDPKLFQ LIKNCLMRT LRQCSMILE FVKHKGVEV KFHGRGKNE ASHYCGQCE IEVFNILFI REQEKRHVV HCMDCARKQ SPNLEGFVC LEEYKMEEL TEVYDNFTL YPTVSATAE QYRLM
- >TC031464 RA
atgtcatca aggcaacaa caacaaaac agttcgcaa caatcgaaa accgccact caatcgtat caaaaaccc tataatcaa gcttcgacc ccgcaaggc ccgccaccg ccttacccg tccccaggc ggcggctcc gtgaaacaa ttcaaaacc gaagtcgtc gaacaaaaa cccgccccc caacctcaa actcacccc ttcactctc actcaacag caactccaa atgttgaat tactttcaa caaaacata aacaatcta acgcctgcc caagaaaat atgagactg caactccaa catcagcac cggttgatg caacaacac caacaacag ttgagacac caaagacaa gcccagccc ccgcgacag ggctccacg ccgcctgcc tacaacgga aacaacttc cagggagat cccccgact ttgaatccc cccaaaaac ctttcgccc ccctccaat ggagacaaa ctagtccaa ccaacacca aatgtgatc ccgagctgc gcgagggac ataaccatg cccgtttcg gggcaaaat tcagcagac atggacgtg agtgaggaa gaattgaag gacttcctc tcgcagaag gatctagca acgacgctc gccgaaaat ctgctcaag cacttcggc tccgacgac atcgacatc aaagaggaa caggagcaa agtgattca tcgaaaccg gaaatggac acgaaacga cgaatcaaa tcgccaccg cacgatagc atcctaact atcaaatcg gaaccgcct tgggaagtc gattcgctg cattctgtg gaaaaacga ccggaaatc gaatacacg atcgacatg gacgccaaa acaatccta agactgtgc aaaggtgag ggcatcaag ggcgaaatc agcaactcg ttaatatca gatcgagcg ccgccccct tcacccccc gacccccca gcaataaaa ctaaaccac caacagtta cttccgccc accccttcg gtctactta gataataaa aaacacgcg tttagtcca cagttacag gagttttgc ctcaaacac cccatagct gtagtacgc ggattagcc tcggcgtta aaactcgac ttaggctta ttttcgact aaaacacta gttgaggct aatcccgat cacagtgtt gaagttagg acgcaaata cagcaacca tccgacgaa aactgggac ccacagtcc gggaagaag gtctgggct tgtatctcg cataggtcg cacacgacc attgccaga tatgcgcag taccaagcc tccagcttt aaggaaagt ttaaaggaa gagagggat aaggccacc agtggcttg tccgactcg gattccaag gactcggtc aattttccc aagaggagg cggctctgc aacatcccg cccttggcc aacaccaag ggctgcaac cagcccaag atgctaaag tttgggacg aacgtggac ttgtcggat gagaaaaag tggaaggcg cagcttcag gagttgatg aaactgccg gcgtttgct cgagttgtc tcggccgga aacatgctg tcgcacgtg ggccacgtg atcctcggc atgaacacg gtccagctg tacatgaag gtacctggc agtcggact ccgggccac caggaaaac aacaacttt tgctcgatt aacataaac atcgggcca ggtgattgc gaatggttt gcggtccct gacgcatat tggggcgca atttgcgcc ctgtgcgaa aagaaccaa ataaactac cttcatggg tcgtggtgg cccgtcctg gaggactta tacaatgcg aatatcccc gtttatcgg tttttgcaa cgtcctgga gacctggtc tgggtcaat gctgggtgc gtccattgg gtgcaagct gtgggctgg tgcaacaac atcgcctgg aacgtgggc cctctcaca gcgcggcaa taccaactc gctatcgag cgatacgag tggaataaa ctgcagagc ttcaagtca attgtgcca atgttgcac ttgtcgtgg aacttgtcg cggaatatc aaagtttcc gacccgaag ttgttccag ttgattaag aattgctta atgcggacc ttgcgacag tgttcgatg attttggag tttgtgaaa cataaaggt gtcgaggtc aagttccac ggacgaggg aagaacgaa gcgtcgcat tactgtggc caatgcgag atcgaggtg tttaatatc ttgttcata agggagcag gagaagcgg cacgtggtc cattgtatg gactgcgcg cgcaagcag agcccgaac ctggagggg ttcgtgtgt ttggaggag tacaaaatg gaggagttg acggaagtt tacgataac ttcacttta tatcccacc gttagtgct actgctgag caatatcga ctaatgtag
- >TC031464 RA
aaaaaagaa aagataatg gaaaagaca aaaaagaaa gtgcaatcg cggacacgt gtcggttgt caaactccc taggacttg aatcaaata aactataag ctgttgata gtagtgata gtatttttc gatgataag ataaggtgt gaattggtc taaattatg taaaaatgt tgtaatatc gaacgaact gaatttcgt gagattgtg taaacgtgg gttagggga gaaaaagca ttcactagg cttgaggtg agctgaacc acgtggtga atgcgtttt tctcgcgtg attaattaa acaatagta aaaaatagc gagagttgt tccttaagt aggcaataa ccttctagg cgtagacaa ttgctctcc atagaagaa gcgtggaat cttccgata tcggctgaa atgtcatca aggcaacaa caacaaaac agttcgcaa caatcgaaa accgccact caatcgtat caaaaaccc tataatcaa gcttcgacc ccgcaaggc ccgccaccg ccttacccg tccccaggc ggcggctcc gtgaaacaa ttcaaaacc gaagtcgtc gaacaaaaa cccgccccc caacctcaa actcacccc ttcactctc actcaacag caactccaa atgttgaat tactttcaa caaaacata aacaatcta acgcctgcc caagaaaat atgagactg caactccaa catcagcac cggttgatg caacaacac caacaacag ttgagacac caaagacaa gcccagccc ccgcgacag ggctccacg ccgcctgcc tacaacgga aacaacttc cagggagat cccccgact ttgaatccc cccaaaaac ctttcgccc ccctccaat ggagacaaa ctagtccaa ccaacacca aatgtgatc ccgagctgc gcgagggac ataaccatg cccgtttcg gggcaaaat tcagcagac atggacgtg agtgaggaa gaattgaag gacttcctc tcgcagaag gatctagca acgacgctc gccgaaaat ctgctcaag cacttcggc tccgacgac atcgacatc aaagaggaa caggagcaa agtgattca tcgaaaccg gaaatggac acgaaacga cgaatcaaa tcgccaccg cacgatagc atcctaact atcaaatcg gaaccgcct tgggaagtc gattcgctg cattctgtg gaaaaacga ccggaaatc gaatacacg atcgacatg gacgccaaa acaatccta agactgtgc aaaggtgag ggcatcaag ggcgaaatc agcaactcg ttaatatca gatcgagcg ccgccccct tcacccccc gacccccca gcaataaaa ctaaaccac caacagtta cttccgccc accccttcg gtctactta gataataaa aaacacgcg tttagtcca cagttacag gagttttgc ctcaaacac cccatagct gtagtacgc ggattagcc tcggcgtta aaactcgac ttaggctta ttttcgact aaaacacta gttgaggct aatcccgat cacagtgtt gaagttagg acgcaaata cagcaacca tccgacgaa aactgggac ccacagtcc gggaagaag gtctgggct tgtatctcg cataggtcg cacacgacc attgccaga tatgcgcag taccaagcc tccagcttt aaggaaagt ttaaaggaa gagagggat aaggccacc agtggcttg tccgactcg gattccaag gactcggtc aattttccc aagaggagg cggctctgc aacatcccg cccttggcc aacaccaag ggctgcaac cagcccaag atgctaaag tttgggacg aacgtggac ttgtcggat gagaaaaag tggaaggcg cagcttcag gagttgatg aaactgccg gcgtttgct cgagttgtc tcggccgga aacatgctg tcgcacgtg ggccacgtg atcctcggc atgaacacg gtccagctg tacatgaag gtacctggc agtcggact ccgggccac caggaaaac aacaacttt tgctcgatt aacataaac atcgggcca ggtgattgc gaatggttt gcggtccct gacgcatat tggggcgca atttgcgcc ctgtgcgaa aagaaccaa ataaactac cttcatggg tcgtggtgg cccgtcctg gaggactta tacaatgcg aatatcccc gtttatcgg tttttgcaa cgtcctgga gacctggtc tgggtcaat gctgggtgc gtccattgg gtgcaagct gtgggctgg tgcaacaac atcgcctgg aacgtgggc cctctcaca gcgcggcaa taccaactc gctatcgag cgatacgag tggaataaa ctgcagagc ttcaagtca attgtgcca atgttgcac ttgtcgtgg aacttgtcg cggaatatc aaagtttcc gacccgaag ttgttccag ttgattaag aattgctta atgcggacc ttgcgacag tgttcgatg attttggag tttgtgaaa cataaaggt gtcgaggtc aagttccac ggacgaggg aagaacgaa gcgtcgcat tactgtggc caatgcgag atcgaggtg tttaatatc ttgttcata agggagcag gagaagcgg cacgtggtc cattgtatg gactgcgcg cgcaagcag agcccgaac ctggagggg ttcgtgtgt ttggaggag tacaaaatg gaggagttg acggaagtt tacgataac ttcacttta tatcccacc gttagtgct actgctgag caatatcga ctaatgtag caggtttaa tttgaagtg ctatctttc agctaccac tgccacttg taacggatg tacaactta atgttaata cttgcttat taaatgtaa attcgagtg ataatgtaa aatgtagat aagtgtgta cataatatt ttcaatttt gtagattgc cgataagtt aatgttaca tcttaatac agtgatctc ataacagtt atttataag tgtacatat aaaaataaa tatatttgt acgatatat ttattttgg agtcaagga ataggatga ttcgaatca tcattttcg ttgttatgg atgaaacca tattttcac tgccaaaaa ttatatgta aatgactgt ttttacgta attttttaa gtctgtacg tattgtata attcaaagt ttttttaaa ggcagcaaa ttcggttat tgcgtgtat tcgttcact gccactttg tgatacatt tctacccga ataccactt tatatgttc atagacaga gcctgggtt cgaacagtg ccttcttca ataaattat ctaaacaac attgtgaaa aaattcata ttttctcca gtcatgacc aattacaaa tacagttac attgtgtaa atatgatga tgtattagg aaataaatc aggaaactg gctcaattt tctaagtaa ggtaatccc tatttcctc tgttcatat agctgtgta ttttatatc atgaaaagt ctgatttca ctttatgta ttatatagt ttcaattgt agtttagat actacctaa acttgtatt tatgattaa ttaaataaa acgttatat ttgga
