Status
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Gene information
General information
TC004683 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 222561..227243
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004683
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00753

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 10 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004683 RA
      MAPPPVVTG LSPNEGLPG TRITIRGEN FGSKPTDLI 
      GLSICGFDC LLSAEWKSP NKIIAISGP VKGKGDVIV 
      TTRLGGVGT CNVTFKGYH ETIGPMKES AVWVEETTP 
      LFVWGRHSL SPSSYQQED PLGLSVEGN DKKFPEDDL 
      MTYFPDKSG DIASGNFSP GWFLLENHH ATSFNDLQA 
      GLNFLRRKV EGQKEGQLS FLKANIGSV MDQIDTLVN 
      LKEKIEGDV AKFGTEPTL KLEKSIKES EREGRKLFD 
      DVLARRDRA EKTRNALNV LARFRFLFC LPCVIDRNI 
      KKGEYDIVI NDYIRVKNL FNKTDVPIF KSALNEVDK 
      RIADLKKKL LTDLQTMPI TVEQQKRLI RYLVNLDCT 
      EDPAWRAIE SRSAYINKN IKQIYGEHN VEKPDPRSK 
      SNSTSKYSK YNPPPQNDL NTVPANVNF IDEICLSIS 
      ETFPDLWKI GQAYFSGEL RVKIEPGRH VEFKHIVMT 
      IMESFCKSI RSSLIPNTL DKADKSALG SWTIPELEE 
      VAPFLPEIL RCVRSTYIA LIKLDLPSE ALDIVSSLL 
      LDLRIHCMS TLFKQAAEQ IKQLNENWK IDFSGNHVG 
      ITELPIKFE RIIQDVIQI VKESVLSTE QRETPLLEN 
      PTAHKELDK QIEAVLVSF HNVLNNLSS DESCDDDED 
      TSPVVSQLI GTPINGYRT NYTNNNMPI WEHRLLITL 
      SNCLYAKNV VFDTILRKF KESGFPAPE TPVKNAKDK 
      LDVLEKTIL EKYLEQKSD PLVGTIEPS MYLGRFDWD 
      IEITPNNIR PYAKECINN VIHVHSEVN SISSSLLES 
      VLPQVVLTI AEELYRLMS CVQKFSKGG AQQARADIS 
      ALQIFFDHY STPKARSYF QEALDIIPP LENSEATVV 
      QDILRQCRA RMRIQLLCL TSHANGPVK KRSH
    CDS sequence
    • >TC004683 RA
      atggcaccg ccccctgtt gtaacaggg ctgagccct 
      aacgagggg cttccaggg actaggatt acaatacga 
      ggggagaat ttcgggtca aaacccacc gatttgata 
      ggtttgtca atttgcggc tttgattgt cttttgtcg 
      gccgagtgg aaatccccc aataaaata atcgcgatt 
      tcggggccc gtgaagggc aaaggtgat gtgatagtg 
      acgacaaga ctggggggc gtaggcact tgtaacgtc 
      acatttaaa gggtaccac gagaccatc ggtccaatg 
      aaagagagt gcggtttgg gtagaagaa accaccccc 
      ttgtttgta tgggggcgc cactccttg tcccccagt 
      agttaccaa caggaggac ccgttaggt ctctccgtt 
      gaaggcaat gacaaaaaa ttccccgaa gatgattta 
      atgacatac tttcccgat aaatcgggc gacattgcc 
      tctggaaat ttttcacca gggtggttc cttctggaa 
      aatcatcac gcaacgtcg ttcaatgat ctacaggcc 
      gggttgaat tttttgagg cgcaaggtg gaagggcag 
      aaagagggc caattgtcg tttttgaag gcaaatatt 
      ggctcggtt atggaccaa attgacact cttgtcaat 
      ttaaaggag aaaattgag ggcgatgtc gccaaattc 
      gggaccgag ccaacgctc aagctcgaa aaatcaata 
      aaagaatct gaaagggaa ggtaggaaa ttgtttgac 
      gacgtgcta gcgcggcga gaccgagcc gaaaagact 
      cgaaacgcg ctaaacgtg ttagcccgg tttaggttc 
      ttgttttgc ctcccgtgt gtaatagat aggaacata 
      aagaaaggt gaatacgat atcgtaatc aacgattac 
      atacgagtc aaaaattta ttcaacaaa actgatgtg 
      cctatattt aagtctgca cttaatgaa gtggacaaa 
      cgaatagcc gaccttaaa aagaagctc cttaccgac 
      ttacaaaca atgccaata actgtagag caacagaaa 
      cgcttaatt agatactta gtgaattta gactgtacc 
      gaggaccca gcttggcgg gccatcgag agccgctca 
      gcttacatc aacaaaaac ataaagcag atttatggc 
      gagcacaat gtcgagaaa cccgatccg agatcgaag 
      tcaaactcg acgtcgaaa tactcgaaa tacaaccct 
      ccgcctcag aacgactta aacacagtt ccggcaaac 
      gtgaatttt attgacgaa atttgcctc agtatttcg 
      gaaacgttc ccagacttg tggaaaatc ggccaggct 
      tatttctcg ggtgaattg cgagtcaaa atcgaaccc 
      ggacgccac gtcgaattt aaacacatt gttatgaca 
      ataatggag agtttttgc aaatcaatc cggtcgtct 
      ttgatcccc aacactctc gataaggcc gataaaagc 
      gcgctcggg tcttggaca atccccgaa cttgaggag 
      gtggcgccg ttcttgccc gaaatttta cgatgtgtg 
      cgctcgact tacatcgct ttaattaag ttagatttg 
      ccgagtgag gctctggat attgtctcg tcgctttta 
      ctcgacttg cggatacac tgtatgagt actttattc 
      aaacaagcg gccgaacaa atcaagcag ttgaacgag 
      aactggaaa attgatttt agtgggaac cacgttggg 
      ataaccgaa ctgccaatc aaattcgag cgcatcatc 
      caagacgtg atccaaatc gttaaggaa tcggtttta 
      tcgacggag cagcgcgaa acacctctc ttggaaaat 
      ccgactgcg cataaagag ttggataaa cagatcgaa 
      gcagtttta gtgtcgttt cacaacgtt ttgaacaat 
      ttatcgtcg gatgagagt tgtgatgac gatgaggat 
      acgtcacct gtcgtctcg caactaatc ggaactccg 
      atcaatggc tatagaacg aattatacc aataataat 
      atgccgatt tgggagcac cgtctttta ataacacta 
      tcgaattgc ctctatgcc aaaaacgtg gtctttgac 
      accatttta cggaagttc aaggagagt ggtttcccg 
      gcaccggaa acgccagtc aaaaacgcc aaagataag 
      ttggatgtg cttgaaaaa acgatcctt gagaagtat 
      ttggaacag aaaagtgat cctttagtt ggcacaatc 
      gaaccctcg atgtattta ggccgattt gattgggac 
      attgaaata acccccaat aatataagg ccctatgca 
      aaggagtgc atcaataac gtgatccat gtccactct 
      gaagttaac agtatttcg tcttctcta ctagaaagc 
      gtacttcct caagtagtc ctaacgata gccgaagag 
      ttataccga ctgatgtcc tgtgttcag aagtttagc 
      aaaggcggg gcacaacaa gccagagct gacataagc 
      gcactccaa atttttttc gaccattac tcgacacct 
      aaagctcgg agctacttt caggaagct ttagatatt 
      attcctccg cttgaaaac tccgaagcc actgtggtg 
      caggatatt ttgagacag tgcagggcc cgcatgagg 
      atacaactc ctgtgtcta acgtcacac gcaaacggg 
      ccagtcaag aaaagatct cattaa
    mRNA sequence
    • >TC004683 RA
      atcaatttg tgaaaagtg acgaaatca aaaaagaaa 
      aaacacgca gcgaattga aattttatg taaattggg 
      ctgcactta gcgaaattg gacaattgt aacatgatg 
      caccgcaat aaattttca ttaagttaa tacgcactt 
      aaataagac tttatttcc gcggaggaa cggaaatac 
      gcatcttgg cttcaaatt tagcgccgt ttggctggt 
      ttgaagttc gcccaaaca tggcgttgg ctgtgttgt 
      tgtcgtaat gtaaacgtc aaatgtcag agttgaaac 
      aaaaatatt tttaattta tttatttga atcagaatg 
      cagtaacga attggttta attaatcac atttgattt 
      ggagtatcg ccagtatga gtttttgag acggaaagt 
      gcttagttt tttagttag gaaatggca ccgccccct 
      gttgtaaca gggctgagc cctaacgag gggcttcca 
      gggactagg attacaata cgaggggag aatttcggg 
      tcaaaaccc accgatttg ataggtttg tcaatttgc 
      ggctttgat tgtcttttg tcggccgag tggaaatcc 
      cccaataaa ataatcgcg atttcgggg cccgtgaag 
      ggcaaaggt gatgtgata gtgacgaca agactgggg 
      ggcgtaggc acttgtaac gtcacattt aaagggtac 
      cacgagacc atcggtcca atgaaagag agtgcggtt 
      tgggtagaa gaaaccacc cccttgttt gtatggggg 
      cgccactcc ttgtccccc agtagttac caacaggag 
      gacccgtta ggtctctcc gttgaaggc aatgacaaa 
      aaattcccc gaagatgat ttaatgaca tactttccc 
      gataaatcg ggcgacatt gcctctgga aatttttca 
      ccagggtgg ttccttctg gaaaatcat cacgcaacg 
      tcgttcaat gatctacag gccgggttg aattttttg 
      aggcgcaag gtggaaggg cagaaagag ggccaattg 
      tcgtttttg aaggcaaat attggctcg gttatggac 
      caaattgac actcttgtc aatttaaag gagaaaatt 
      gagggcgat gtcgccaaa ttcgggacc gagccaacg 
      ctcaagctc gaaaaatca ataaaagaa tctgaaagg 
      gaaggtagg aaattgttt gacgacgtg ctagcgcgg 
      cgagaccga gccgaaaag actcgaaac gcgctaaac 
      gtgttagcc cggtttagg ttcttgttt tgcctcccg 
      tgtgtaata gataggaac ataaagaaa ggtgaatac 
      gatatcgta atcaacgat tacatacga gtcaaaaat 
      ttattcaac aaaactgat gtgcctata tttaagtct 
      gcacttaat gaagtggac aaacgaata gccgacctt 
      aaaaagaag ctccttacc gacttacaa acaatgcca 
      ataactgta gagcaacag aaacgctta attagatac 
      ttagtgaat ttagactgt accgaggac ccagcttgg 
      cgggccatc gagagccgc tcagcttac atcaacaaa 
      aacataaag cagatttat ggcgagcac aatgtcgag 
      aaacccgat ccgagatcg aagtcaaac tcgacgtcg 
      aaatactcg aaatacaac cctccgcct cagaacgac 
      ttaaacaca gttccggca aacgtgaat tttattgac 
      gaaatttgc ctcagtatt tcggaaacg ttcccagac 
      ttgtggaaa atcggccag gcttatttc tcgggtgaa 
      ttgcgagtc aaaatcgaa cccggacgc cacgtcgaa 
      tttaaacac attgttatg acaataatg gagagtttt 
      tgcaaatca atccggtcg tctttgatc cccaacact 
      ctcgataag gccgataaa agcgcgctc gggtcttgg 
      acaatcccc gaacttgag gaggtggcg ccgttcttg 
      cccgaaatt ttacgatgt gtgcgctcg acttacatc 
      gctttaatt aagttagat ttgccgagt gaggctctg 
      gatattgtc tcgtcgctt ttactcgac ttgcggata 
      cactgtatg agtacttta ttcaaacaa gcggccgaa 
      caaatcaag cagttgaac gagaactgg aaaattgat 
      tttagtggg aaccacgtt gggataacc gaactgcca 
      atcaaattc gagcgcatc atccaagac gtgatccaa 
      atcgttaag gaatcggtt ttatcgacg gagcagcgc 
      gaaacacct ctcttggaa aatccgact gcgcataaa 
      gagttggat aaacagatc gaagcagtt ttagtgtcg 
      tttcacaac gttttgaac aatttatcg tcggatgag 
      agttgtgat gacgatgag gatacgtca cctgtcgtc 
      tcgcaacta atcggaact ccgatcaat ggctataga 
      acgaattat accaataat aatatgccg atttgggag 
      caccgtctt ttaataaca ctatcgaat tgcctctat 
      gccaaaaac gtggtcttt gacaccatt ttacggaag 
      ttcaaggag agtggtttc ccggcaccg gaaacgcca 
      gtcaaaaac gccaaagat aagttggat gtgcttgaa 
      aaaacgatc cttgagaag tatttggaa cagaaaagt 
      gatccttta gttggcaca atcgaaccc tcgatgtat 
      ttaggccga tttgattgg gacattgaa ataaccccc 
      aataatata aggccctat gcaaaggag tgcatcaat 
      aacgtgatc catgtccac tctgaagtt aacagtatt 
      tcgtcttct ctactagaa agcgtactt cctcaagta 
      gtcctaacg atagccgaa gagttatac cgactgatg 
      tcctgtgtt cagaagttt agcaaaggc ggggcacaa 
      caagccaga gctgacata agcgcactc caaattttt 
      ttcgaccat tactcgaca cctaaagct cggagctac 
      tttcaggaa gctttagat attattcct ccgcttgaa 
      aactccgaa gccactgtg gtgcaggat attttgaga 
      cagtgcagg gcccgcatg aggatacaa ctcctgtgt 
      ctaacgtca cacgcaaac gggccagtc aagaaaaga 
      tctcattaa ctcgacaag cttagtaac tgtgattct 
      attgctcat tatttttcc aataactat ttgtacata 
      aagtaagat gccttggag acgaagtaa taatagggt 
      gtatttcgt gtacagttt cagttgtaa tttatcgtg 
      gttaacttt tgttctagg gtgtacaat aattttgtg 
      taaaatgta ataaaagat taacttatt t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY