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Gene information
General information
TC004679 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 182201..185485
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004679
    iBeetle screen iB_00752

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    remarks: later than schedule,
    Pupal morphology
    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    Fertility
    egg production: number <50% of expected -
    tropharium number decreased - Number of animals showing the phenotype: 3

    tropharium size shortened - Number of animals showing the phenotype: 3
    tropharium shape irregular - Number of animals showing the phenotype: 3
    vitellarium size decreased - Number of animals showing the phenotype: 3
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 3
    vitellogenic egg chamber not present - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004679 RA
      MRVPDLGMG ELLHDNLFG LFEAMSAIE MMDPKMDAG 
      MVCNRGARK AKTLSQTIE EGILQLDDF SYEKMIGII 
      DTTLACIVS WLEGHSLAQ TVFTNLYLH KPYSIEDRT 
      MKAFCLMVY KLLEIIKHF VHKAMVYEE EDFQPMQYG 
      YHLNPDISE QRIIGMLRE VEEELHRKI RSKQSSSEI 
      QAVHARIKF MRVFYQALT LMRREDQPP AITDCQRLL 
      LTASDQLII MKETVDLGL QSGDNELIP GFEPLINQR 
      LLPPTFPRY TKIKPRGEA LNYFSDMVD RFKTITKIQ 
      SITSLHQVI DFFIDFSKT SPCLLSRSA LQLLYQGTN 
      PCALRDILK EAVKTFICP PALVSKSIL SNQQAKKYV 
      ETFLAHCTR PFASFLQLC GHNRARQRD KLAHLLEEF 
      TNLQDEAER VDAYLHNIS VNSTSRPHL ACFGTWILY 
      HTLKIMIMY LLAGFELEL YSRHEYYYI YWYLYEFLY 
      GWLVSALSR ADSFLVENE LINELQKSK NTSKKKPKH 
      KRKPRPYNR DIVYYQALQ NMCGGYYKA LMGFRLDGK 
      LTMPHPQFD NEQVRYEHR FAPFQNLLT PPPVQYSEF 
      REMTSVERL QQPVDSIYF YVTGCKHFH QARQLLENI 
      QADSEIIDL IKISKTNFI VLKLLAGGH KKDSVTPPE 
      FDFSVNRYF PIIKLT
    CDS sequence
    • >TC004679 RA
      atgagagtt ccagactta ggcatgggc gagttattg 
      cacgacaat ttgtttggg ctttttgag gccatgtca 
      gcgattgag atgatggac cccaaaatg gacgctggg 
      atggtttgt aacaggggg gcgcgcaaa gccaagaca 
      ctcagccag acaattgag gagggtata ttgcaattg 
      gatgatttc tcgtacgaa aaaatgatc gggattatt 
      gacactaca ctggcttgt atcgtttcg tggttagaa 
      ggtcattcg ttggctcaa accgttttc actaatctt 
      tatttacat aagccttac agcatagaa gacaggact 
      atgaaagct ttctgcctc atggtgtac aaactattg 
      gaaataatt aagcatttt gtgcacaaa gccatggtt 
      tacgaagaa gaggacttc caaccgatg caatacggc 
      taccacctc aatcctgac atctccgaa cagagaatt 
      atcggaatg ttacgcgaa gttgaagag gaattacat 
      cggaaaatt cggtcgaaa caatcatct agtgaaatt 
      caagctgtt cacgcacgc attaaattc atgcgagtg 
      ttttaccaa gctttgacg ctgatgagg cgcgaggac 
      cagccccct gccatcacc gactgccaa agactgctg 
      ctcacagcg tctgaccaa ttaattatc atgaaagaa 
      acggtcgat ttggggctc caatcaggt gataacgag 
      ttaattccg ggctttgaa cccttaata aaccagcga 
      ttgttaccc ccaacgttt cctcgctac accaaaatt 
      aagccgagg ggcgaagcc ctcaactat ttctcagac 
      atggtcgac aggtttaaa accataaca aaaatccag 
      tcaataacg tcgctacac caagttata gatttcttc 
      attgatttt agtaaaact agtccgtgt ttgctgtct 
      cggtcagct cttcagtta ctatatcaa gggacaaac 
      ccgtgcgcc ttgcgtgac attttaaag gaagcagtg 
      aagacgttc atatgccca cctgcgttg gtctcaaaa 
      tcgattctt tcgaaccaa caagccaaa aaatacgtg 
      gagacgttt ttggctcat tgtacgcga cctttcgct 
      agtttttta caactttgt ggccacaac agggccaga 
      caaagagac aagttggct catttactt gaagagttt 
      actaatcta caagatgag gctgaacga gtcgacgct 
      tatttacat aatatatcg gtcaattcg acctcacgg 
      ccacatttg gcatgtttc ggtacttgg attttgtac 
      cacacgctc aaaattatg attatgtac ttactggca 
      gggttcgag ttggaactc tacagcagg cacgaatat 
      tactacatt tattggtac ttgtacgag tttttatac 
      gggtggctg gtgtcggcc ctttcacgg gctgatagt 
      tttctagtc gaaaacgag ctcataaac gagttacaa 
      aagtcgaaa aacacgtcg aaaaagaaa ccgaaacac 
      aagagaaaa ccaaggccc tacaatcgg gacattgtt 
      tattaccag gcgttacag aacatgtgc ggcggttac 
      tacaaagct ttgatgggc ttccgattg gacgggaaa 
      ctgacgatg ccccacccc cagttcgat aacgaacaa 
      gtgcggtat gagcaccgt ttcgccccc tttcagaac 
      ctcctcacg ccgccccca gtccagtac tcggaattt 
      cgggagatg acgtccgtt gagaggttg caacaacca 
      gttgatagt atttatttt tacgtaacg gggtgcaaa 
      cacttccac caagcgcgc caattgctg gagaacatc 
      caagctgat agcgaaata atcgacctg attaaaata 
      tccaaaact aattttatt gtgttgaag ttgctagcc 
      ggggggcac aagaaagac tcggtgacg cccccagag 
      ttcgatttc tcggtcaat cgctacttt ccgataatc 
      aaactgact taa
    mRNA sequence
    • >TC004679 RA
      caacaattt tttgttaaa tgagagttc cagacttag 
      gcatgggcg agttattgc acgacaatt tgtttgggc 
      tttttgagg ccatgtcag cgattgaga tgatggacc 
      ccaaaatgg acgctggga tggtttgta acagggggg 
      cgcgcaaag ccaagacac tcagccaga caattgagg 
      agggtatat tgcaattgg atgatttct cgtacgaaa 
      aaatgatcg ggattattg acactacac tggcttgta 
      tcgtttcgt ggttagaag gtcattcgt tggctcaaa 
      ccgttttca ctaatcttt atttacata agccttaca 
      gcatagaag acaggacta tgaaagctt tctgcctca 
      tggtgtaca aactattgg aaataatta agcattttg 
      tgcacaaag ccatggttt acgaagaag aggacttcc 
      aaccgatgc aatacggct accacctca atcctgaca 
      tctccgaac agagaatta tcggaatgt tacgcgaag 
      ttgaagagg aattacatc ggaaaattc ggtcgaaac 
      aatcatcta gtgaaattc aagctgttc acgcacgca 
      ttaaattca tgcgagtgt tttaccaag ctttgacgc 
      tgatgaggc gcgaggacc agccccctg ccatcaccg 
      actgccaaa gactgctgc tcacagcgt ctgaccaat 
      taattatca tgaaagaaa cggtcgatt tggggctcc 
      aatcaggtg ataacgagt taattccgg gctttgaac 
      ccttaataa accagcgat tgttacccc caacgtttc 
      ctcgctaca ccaaaatta agccgaggg gcgaagccc 
      tcaactatt tctcagaca tggtcgaca ggtttaaaa 
      ccataacaa aaatccagt caataacgt cgctacacc 
      aagttatag atttcttca ttgatttta gtaaaacta 
      gtccgtgtt tgctgtctc ggtcagctc ttcagttac 
      tatatcaag ggacaaacc cgtgcgcct tgcgtgaca 
      ttttaaagg aagcagtga agacgttca tatgcccac 
      ctgcgttgg tctcaaaat cgattcttt cgaaccaac 
      aagccaaaa aatacgtgg agacgtttt tggctcatt 
      gtacgcgac ctttcgcta gttttttac aactttgtg 
      gccacaaca gggccagac aaagagaca agttggctc 
      atttacttg aagagttta ctaatctac aagatgagg 
      ctgaacgag tcgacgctt atttacata atatatcgg 
      tcaattcga cctcacggc cacatttgg catgtttcg 
      gtacttgga ttttgtacc acacgctca aaattatga 
      ttatgtact tactggcag ggttcgagt tggaactct 
      acagcaggc acgaatatt actacattt attggtact 
      tgtacgagt ttttatacg ggtggctgg tgtcggccc 
      tttcacggg ctgatagtt ttctagtcg aaaacgagc 
      tcataaacg agttacaaa agtcgaaaa acacgtcga 
      aaaagaaac cgaaacaca agagaaaac caaggccct 
      acaatcggg acattgttt attaccagg cgttacaga 
      acatgtgcg gcggttact acaaagctt tgatgggct 
      tccgattgg acgggaaac tgacgatgc cccaccccc 
      agttcgata acgaacaag tgcggtatg agcaccgtt 
      tcgccccct ttcagaacc tcctcacgc cgcccccag 
      tccagtact cggaatttc gggagatga cgtccgttg 
      agaggttgc aacaaccag ttgatagta tttattttt 
      acgtaacgg ggtgcaaac acttccacc aagcgcgcc 
      aattgctgg agaacatcc aagctgata gcgaaataa 
      tcgacctga ttaaaatat ccaaaacta attttattg 
      tgttgaagt tgctagccg gggggcaca agaaagact 
      cggtgacgc ccccagagt tcgatttct cggtcaatc 
      gctactttc cgataatca aactgactt aattttttt 
      tgctgtgat gaaggggct ttttttgca acttgggcg 
      gaaatttcg gcttcaatt gtttcttct ttatttgac 
      gcggggtta aatttggtt cgttgggcg gcatagtgt 
      ttggggtta gtctcgtgg ttctgaata tattcgagt 
      cgattttat ttattagga tgtcggaaa attcatcat 
      cgtgttttt ttatctcgt cgatttttt tatgtaggg 
      gcagaattg ccggcgcca gcaatcgaa agaccatct 
      gcaaataaa tttttatca agatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY