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Gene information
General information
TC004676 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid1: 154281..166655
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004676
    iBeetle screen iB_00751
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004676 RA
      MAKRVIILI LVVDFLSLV TSVPQIFTT SYGFCQFKF 
      TGKKTIALP DKLWTIDTS DFILHFSLC GSIQEVCKN 
      ETSSCLFLK NSNKMIDLG HEFGSASED TILMSQNGA 
      NCTSDQNYT LSISFVCSD SIKPKPRLM AQDAESECN 
      FEVTMMRPD CQPRCTETV QGSELIDLR NFPKNVQVH 
      ADSKHFSLS LCGSNKNCP GGVSACEIN PDGSTTPLA 
      SIESQILVY DQGKNELKA RGRFRERGE TRIVEVLIK 
      CNWGSELAN PVYKKVANQ GKRYRFEIE SSYGCVKLP 
      YSCQLSSLD NLNYDLRNL YTTHGWAVT GAPHGKIFV 
      NICGPLKQP PNLCSAQHS QVCHVTDNV YVNRGSILS 
      LFEARDDSL RARFESGST CTENTTRTY STEVEFKCS 
      KTEKGPQFK HIENCVMFL QWETPHACP LDYYKSQNC 
      YVKDRVSNR NLKNLHSDT DRIYPLSPS TNSKLIYNL 
      CGPIHEPCN GHKNASFCL KTGQKESVL GWDTREVIS 
      ENGRLFMQF SGENRAEIF VNLVCRYQS SPPDLQVTE 
      DSRKYNLTV FTPLACIAQ GVFPECVYH SGNFTYDLS 
      PLVLKNKNY VIPGGQGTE IIFNVCGPI ITGSGALCR 
      GTTMFCYRN TTALNIKNQ YVSLGGVSG PRLLNNSLI 
      LEAPMGEFC REVGYFKSV MRFKCSKTN VLPRFVSKE 
      SCVYNFIWE TPQACPQIR NCTVGHQPG LTFDLNQIK 
      NKIVRLDID GISSKYEFD LCKDFYTTI SNSNKTNRL 
      RRDYVITNR NSFVILDLY EKCANATPL TKVIFKLEC 
      SDKDSHFEQ TAFENCVLN MTLKTHAVC LSHHTTTSA 
      LQKLETNCT VVNGNTGYE FNLIKLKAE DILQKFQIN 
      FDQTSALCS GLFCKNGLG VVPQICPKV AFNYTSQFV 
      TLSYLGVEC DDFSGNYEF EVLLKCNRT RTGARISQD 
      AECKSVISY DLPEACSLL KREIVASDP TAAIAGAMR 
      LRKNFRVSS PQNVYIATA VLLLVCCLS CSAATVGGK 
      RPASPSIAT NPAKKQATV MEPAFANAG QNAGIQIWR 
      IENFKPVAY PKNEYGKFY SGDSYIVLS TKINKRGEK 
      SWDIHYWLG SQTSQDEAG SAAIFAVQL DDQLGGVPI 
      QYRETQEHE SQLFLSYFK NGVRYLPGG VASGFTHVD 
      PNAFEKRLF QVKGSRNIR VKQVDPVVS SMNKGDCFI 
      LDVGRDIYV YVGAKSKRV ERLKAISAA NQIRDQDHA 
      GKAKVNIID EYSPDHDFA EFFSALGSG SAASVPDES 
      AGGDDAQFE SNQERVVSL YRVSDNSGS LKVDLVAQK 
      PLQQSLLDG NDCFILDSS DSNIFVWIG KKCNNKEKQ 
      EAMVKAQNF LTSKKYPAW THVQRIVEG AEPTAFTQY 
      FQSWRNRNE LHTRLIRSP STEKYNSFE TRLFHAEIK 
      AKTNKFEVE EIIDFEQSD LNEDDVMLL DVGKDLFVW 
      IGNGASVKE KAKANDLAK MHLKKYGRE DTAVTSIAQ 
      GHEPEAFTS VFPSWNPDF WDSLESYDD IKAKIAEDN 
      KAL
    CDS sequence
    • >TC004676 RA
      atggcaaaa agggtaata attttaatc ttagttgtc 
      gactttttg tcacttgtt acatcagtt cctcaaatt 
      tttacgact agttatggt ttttgccaa tttaaattc 
      accggaaag aaaacaatt gcattgccg gataaattg 
      tggacaatt gatacttcg gattttatt ctgcatttt 
      tcgctatgt ggatcaata caagaggtt tgcaaaaat 
      gagacgtct tcgtgcctc tttctaaaa aactcaaat 
      aagatgatt gatttaggt cacgagttt ggttcagcc 
      tccgaggac acaattctc atgtcgcaa aacggcgca 
      aattgcacc tctgaccag aactacacc ctttccata 
      tccttcgtt tgttccgat tcaattaaa ccgaaacct 
      cgcctgatg gctcaggac gcggaaagt gagtgtaat 
      ttcgaggtg accatgatg cgccccgat tgccaaccg 
      aggtgcacc gaaaccgtc caagggtcc gagttaatc 
      gaccttcgg aattttccc aaaaacgta caagtgcac 
      gcggactca aagcacttc agtttgagc ctctgcggc 
      tcaaacaaa aactgtccc gggggcgtc agcgcttgc 
      gaaatcaac ccggacggc tccacaacc cccctggcc 
      agcatcgag tcgcaaatc ctggtctac gaccaaggc 
      aagaacgag ctcaaggcg agggggcgc tttcgcgag 
      cgcggggaa actagaatt gtcgaagtt ttgattaaa 
      tgcaactgg gggagcgaa cttgccaac cctgtttat 
      aaaaaagta gcaaaccag ggaaagcgg taccgattc 
      gaaattgag tcgtcgtat ggctgtgtc aaattgcca 
      tacagttgt caactatca agtcttgat aacctgaat 
      tatgacctc agaaatctt tatacgaca cacgggtgg 
      gcggtcacg ggggccccc catggtaaa attttcgtc 
      aacatttgt ggaccactc aaacaacct ccaaatctc 
      tgctccgcc caacattcg caagtgtgc cacgtgact 
      gataacgta tacgtcaat cggggcagt attttatca 
      cttttcgaa gcccgggac gactcacta agggcccga 
      ttcgagtcg ggctccact tgcacggaa aacaccaca 
      cgtacctac agtaccgaa gtcgaattc aaatgttca 
      aaaaccgaa aaagggcca caattcaaa cacatcgaa 
      aattgcgtc atgtttttg cagtgggag acgccccac 
      gcgtgcccc cttgattat tacaaaagt caaaattgc 
      tacgtcaag gacagggtg tccaaccga aacttgaaa 
      aacctacac agtgacacc gatcgaatt taccctttg 
      tcaccctca accaactca aaattaatt tataattta 
      tgcggaccg atacacgag ccttgcaac ggccacaaa 
      aacgccagc ttttgcctc aaaacgggc caaaaagaa 
      agtgtgcta ggctgggac acgcgtgaa gttatcagc 
      gaaaacggc cgcctgttt atgcaattt tccggcgaa 
      aatcgggcc gaaattttc gttaatttg gtctgcagg 
      taccaaagc tcaccccct gatttgcaa gtgactgaa 
      gatagccgc aagtacaat ttgacagtt tttacgcca 
      ttggcgtgc attgcacaa ggggttttc cccgaatgt 
      gtgtaccat tctgggaat ttcacgtac gacttgtct 
      cctcttgtc ttaaaaaat aaaaattat gtcattccg 
      ggggggcaa ggtaccgaa attattttc aatgtttgt 
      ggtcccatt atcacgggc tcgggggcc ctttgtagg 
      ggcactaca atgttttgt taccggaac accacagca 
      ctcaacatt aaaaaccag tacgtgtca ctgggcgga 
      gtctcaggc ccacgctta ctgaacaat tctctcata 
      ctggaggcc ccaatgggg gagttttgc cgcgaagtt 
      ggttacttt aaatcagtg atgcgtttt aagtgctcc 
      aaaacaaat gttttgcca cgatttgtt tcgaaagag 
      tcctgtgtg tacaatttc atatgggag actccccaa 
      gcttgtccc caaatcaga aattgtaca gtgggacac 
      cagccaggt ttaacattt gacctaaac caaataaaa 
      aacaaaatt gtcagatta gacatcgat ggaatctcg 
      agtaaatac gaatttgac ttgtgtaaa gacttttac 
      acaacaatt tccaattcg aataaaaca aatcgcctc 
      agacgcgac tatgtgatt acgaaccga aactcgttc 
      gtgattttg gatctgtac gaaaagtgt gcaaacgcg 
      acgcctctt accaaagtc atttttaaa ttggaatgt 
      tcggacaaa gacagtcat ttcgagcaa acagcgttc 
      gaaaactgt gtcttaaac atgactctc aagacacac 
      gccgtttgc ctaagccac catacaaca acctcagcg 
      ctgcaaaag ttggaaacc aattgcaca gtcgttaac 
      ggcaacaca gggtacgag tttaatttg attaaactc 
      aaagcggag gacattttg caaaaattc caaattaat 
      ttcgaccag actagcgcg ctttgcagc ggccttttt 
      tgtaaaaat gggctcggg gtggtcccc cagatttgc 
      ccaaaagtg gcgttcaat tacacgtca cagtttgtc 
      acactgtcc tatttgggg gttgagtgt gacgacttt 
      tcaggcaat tacgaattt gaagttctc ttgaaatgc 
      aatcgcacc agaaccggt gcgcgtatt tcacaggat 
      gccgaatgc aaatccgtg atttcgtac gacttacct 
      gaagcttgt tccttgctg aagcgagaa atagtggct 
      tctgacccc actgctgcc atagcaggg gcgatgcgc 
      ttgcgcaaa aactttcgc gtttcgtcg cctcaaaat 
      gtttatata gcaacagcg gttttacta ctagtttgt 
      tgtctgagt tgttcggcg gcaacagtc ggcggtaaa 
      agaccagca tcaccatcg attgcaaca aacccagcc 
      aaaaaacaa gcaacagtt atggagccc gcatttgca 
      aatgccggt caaaacgcc ggaattcaa atttggagg 
      attgagaac ttcaaaccg gtagcttac cccaaaaac 
      gaatacggg aaattctac tcaggagac tcctacatt 
      gtcctaagt acgaaaatc aataaacgg ggcgaaaaa 
      tcatgggat atacactat tggctaggc tcgcagacc 
      agtcaggat gaggcgggt tcggctgca atcttcgca 
      gtccaactc gatgaccaa cttggcggc gttccaatc 
      caataccgg gaaacgcaa gagcacgaa tcccaatta 
      ttcctgtca tatttcaaa aacggcgtc cgctacctc 
      cccggaggc gtcgcctcc ggcttcacc cacgtggac 
      cccaacgcg ttcgaaaag cgcctgttc caagtcaaa 
      ggctctcgc aacatccgc gtgaagcaa gtggacccc 
      gtcgtcagc tcgatgaac aaaggcgac tgctttatc 
      ctcgacgta ggccgcgat atctacgtg tacgttggg 
      gccaaaagc aagcgagtt gagcggcta aaggccatc 
      tcggccgcc aaccaaatc cgcgaccag gaccacgcc 
      ggcaaggcc aaagtcaac atcatcgac gagtacagc 
      cccgatcac gatttcgcc gagtttttc agcgctttg 
      gggagcggc agcgccgcc agcgttccc gatgagtcc 
      gcagggggt gacgacgcc cagttcgag tcgaatcag 
      gagcgcgtg gtgtcgcta taccgcgtt tcggacaac 
      agtggcagc ctcaaggtg gatcttgtt gcccagaag 
      ccgctccag cagtcgctg cttgatggc aacgactgt 
      ttcatcctc gacagctcc gactccaac attttcgtt 
      tggattggc aagaaatgc aacaacaag gagaaacag 
      gaagctatg gtcaaggcc cagaacttc ctcacgtcg 
      aaaaaatac ccggcctgg acgcacgtc cagagaatc 
      gttgaaggg gcagaacct acggctttt acacagtat 
      ttccagtct tggaggaac cggaacgaa ctacatact 
      agacttatc cggtccccg agtactgag aaatacaat 
      tcgttcgaa acgcgtctt ttccacgcc gaaattaag 
      gcgaagacc aacaaattc gaagttgag gaaattatc 
      gatttcgag caaagcgat ttgaatgag gacgatgtc 
      atgctgttg gatgttggg aaggatctt tttgtttgg 
      atcggaaac ggggccagt gtcaaggag aaggccaaa 
      gcgaacgac cttgcaaag atgcatttg aagaaatac 
      gggcgtgag gacaccgca gtgacttcc atagcgcaa 
      ggacacgaa cccgaagcc ttcacctcc gtgttcccg 
      agctggaac cccgacttc tgggactcc ctcgagtcc 
      tacgatgac atcaaagcc aaaattgcc gaagacaac 
      aaagcactg tag
    mRNA sequence
    • >TC004676 RA
      tcgcatgtg ggtttgaca taacctcaa gtttttaga 
      cgtaaatgt gagtgattt atcatcaac catctcgtt 
      tcgcggcct tatcaaatt ccgtgcccc atgcagtga 
      accacatat tcgacatta atccttatt tgcagcttt 
      gacgcattt tttcacggc tttaaaccg gaagtattg 
      catcaaata atgcagata tggtttcgg atctttggg 
      attagttac agcgtttcg aaaaaattg tttttgcca 
      cttaattaa ataatatgg caaaaaggg taataattt 
      taatcttag ttgtcgact ttttgtcac ttgttacat 
      cagttcctc aaattttta cgactagtt atggttttt 
      gccaattta aattcaccg gaaagaaaa caattgcat 
      tgccggata aattgtgga caattgata cttcggatt 
      ttattctgc atttttcgc tatgtggat caatacaag 
      aggtttgca aaaatgaga cgtcttcgt gcctctttc 
      taaaaaact caaataaga tgattgatt taggtcacg 
      agtttggtt cagcctccg aggacacaa ttctcatgt 
      cgcaaaacg gcgcaaatt gcacctctg accagaact 
      acacccttt ccatatcct tcgtttgtt ccgattcaa 
      ttaaaccga aacctcgcc tgatggctc aggacgcgg 
      aaagtgagt gtaatttcg aggtgacca tgatgcgcc 
      ccgattgcc aaccgaggt gcaccgaaa ccgtccaag 
      ggtccgagt taatcgacc ttcggaatt ttcccaaaa 
      acgtacaag tgcacgcgg actcaaagc acttcagtt 
      tgagcctct gcggctcaa acaaaaact gtcccgggg 
      gcgtcagcg cttgcgaaa tcaacccgg acggctcca 
      caacccccc tggccagca tcgagtcgc aaatcctgg 
      tctacgacc aaggcaaga acgagctca aggcgaggg 
      ggcgctttc gcgagcgcg gggaaacta gaattgtcg 
      aagttttga ttaaatgca actggggga gcgaacttg 
      ccaaccctg tttataaaa aagtagcaa accagggaa 
      agcggtacc gattcgaaa ttgagtcgt cgtatggct 
      gtgtcaaat tgccataca gttgtcaac tatcaagtc 
      ttgataacc tgaattatg acctcagaa atctttata 
      cgacacacg ggtgggcgg tcacggggg ccccccatg 
      gtaaaattt tcgtcaaca tttgtggac cactcaaac 
      aacctccaa atctctgct ccgcccaac attcgcaag 
      tgtgccacg tgactgata acgtatacg tcaatcggg 
      gcagtattt tatcacttt tcgaagccc gggacgact 
      cactaaggg cccgattcg agtcgggct ccacttgca 
      cggaaaaca ccacacgta cctacagta ccgaagtcg 
      aattcaaat gttcaaaaa ccgaaaaag ggccacaat 
      tcaaacaca tcgaaaatt gcgtcatgt ttttgcagt 
      gggagacgc cccacgcgt gcccccttg attattaca 
      aaagtcaaa attgctacg tcaaggaca gggtgtcca 
      accgaaact tgaaaaacc tacacagtg acaccgatc 
      gaatttacc ctttgtcac cctcaacca actcaaaat 
      taatttata atttatgcg gaccgatac acgagcctt 
      gcaacggcc acaaaaacg ccagctttt gcctcaaaa 
      cgggccaaa aagaaagtg tgctaggct gggacacgc 
      gtgaagtta tcagcgaaa acggccgcc tgtttatgc 
      aattttccg gcgaaaatc gggccgaaa ttttcgtta 
      atttggtct gcaggtacc aaagctcac cccctgatt 
      tgcaagtga ctgaagata gccgcaagt acaatttga 
      cagttttta cgccattgg cgtgcattg cacaagggg 
      ttttccccg aatgtgtgt accattctg ggaatttca 
      cgtacgact tgtctcctc ttgtcttaa aaaataaaa 
      attatgtca ttccggggg ggcaaggta ccgaaatta 
      ttttcaatg tttgtggtc ccattatca cgggctcgg 
      gggcccttt gtaggggca ctacaatgt tttgttacc 
      ggaacacca cagcactca acattaaaa accagtacg 
      tgtcactgg gcggagtct caggcccac gcttactga 
      acaattctc tcatactgg aggccccaa tgggggagt 
      tttgccgcg aagttggtt actttaaat cagtgatgc 
      gttttaagt gctccaaaa caaatgttt tgccacgat 
      ttgtttcga aagagtcct gtgtgtaca atttcatat 
      gggagactc cccaagctt gtccccaaa tcagaaatt 
      gtacagtgg gacaccagc caggtttaa catttgacc 
      taaaccaaa taaaaaaca aaattgtca gattagaca 
      tcgatggaa tctcgagta aatacgaat ttgacttgt 
      gtaaagact tttacacaa caatttcca attcgaata 
      aaacaaatc gcctcagac gcgactatg tgattacga 
      accgaaact cgttcgtga ttttggatc tgtacgaaa 
      agtgtgcaa acgcgacgc ctcttacca aagtcattt 
      ttaaattgg aatgttcgg acaaagaca gtcatttcg 
      agcaaacag cgttcgaaa actgtgtct taaacatga 
      ctctcaaga cacacgccg tttgcctaa gccaccata 
      caacaacct cagcgctgc aaaagttgg aaaccaatt 
      gcacagtcg ttaacggca acacagggt acgagttta 
      atttgatta aactcaaag cggaggaca ttttgcaaa 
      aattccaaa ttaatttcg accagacta gcgcgcttt 
      gcagcggcc ttttttgta aaaatgggc tcggggtgg 
      tcccccaga tttgcccaa aagtggcgt tcaattaca 
      cgtcacagt ttgtcacac tgtcctatt tgggggttg 
      agtgtgacg acttttcag gcaattacg aatttgaag 
      ttctcttga aatgcaatc gcaccagaa ccggtgcgc 
      gtatttcac aggatgccg aatgcaaat ccgtgattt 
      cgtacgact tacctgaag cttgttcct tgctgaagc 
      gagaaatag tggcttctg accccactg ctgccatag 
      caggggcga tgcgcttgc gcaaaaact ttcgcgttt 
      cgtcgcctc aaaatgttt atatagcaa cagcggttt 
      tactactag tttgttgtc tgagttgtt cggcggcaa 
      cagtcggcg gtaaaagac cagcatcac catcgattg 
      caacaaacc cagccaaaa aacaagcaa cagttatgg 
      agcccgcat ttgcaaatg ccggtcaaa acgccggaa 
      ttcaaattt ggaggattg agaacttca aaccggtag 
      cttacccca aaaacgaat acgggaaat tctactcag 
      gagactcct acattgtcc taagtacga aaatcaata 
      aacggggcg aaaaatcat gggatatac actattggc 
      taggctcgc agaccagtc aggatgagg cgggttcgg 
      ctgcaatct tcgcagtcc aactcgatg accaacttg 
      gcggcgttc caatccaat accgggaaa cgcaagagc 
      acgaatccc aattattcc tgtcatatt tcaaaaacg 
      gcgtccgct acctccccg gaggcgtcg cctccggct 
      tcacccacg tggacccca acgcgttcg aaaagcgcc 
      tgttccaag tcaaaggct ctcgcaaca tccgcgtga 
      agcaagtgg accccgtcg tcagctcga tgaacaaag 
      gcgactgct ttatcctcg acgtaggcc gcgatatct 
      acgtgtacg ttggggcca aaagcaagc gagttgagc 
      ggctaaagg ccatctcgg ccgccaacc aaatccgcg 
      accaggacc acgccggca aggccaaag tcaacatca 
      tcgacgagt acagccccg atcacgatt tcgccgagt 
      ttttcagcg ctttgggga gcggcagcg ccgccagcg 
      ttcccgatg agtccgcag ggggtgacg acgcccagt 
      tcgagtcga atcaggagc gcgtggtgt cgctatacc 
      gcgtttcgg acaacagtg gcagcctca aggtggatc 
      ttgttgccc agaagccgc tccagcagt cgctgcttg 
      atggcaacg actgtttca tcctcgaca gctccgact 
      ccaacattt tcgtttgga ttggcaaga aatgcaaca 
      acaaggaga aacaggaag ctatggtca aggcccaga 
      acttcctca cgtcgaaaa aatacccgg cctggacgc 
      acgtccaga gaatcgttg aaggggcag aacctacgg 
      cttttacac agtatttcc agtcttgga ggaaccgga 
      acgaactac atactagac ttatccggt ccccgagta 
      ctgagaaat acaattcgt tcgaaacgc gtcttttcc 
      acgccgaaa ttaaggcga agaccaaca aattcgaag 
      ttgaggaaa ttatcgatt tcgagcaaa gcgatttga 
      atgaggacg atgtcatgc tgttggatg ttgggaagg 
      atctttttg tttggatcg gaaacgggg ccagtgtca 
      aggagaagg ccaaagcga acgaccttg caaagatgc 
      atttgaaga aatacgggc gtgaggaca ccgcagtga 
      cttccatag cgcaaggac acgaacccg aagccttca 
      cctccgtgt tcccgagct ggaaccccg acttctggg 
      actccctcg agtcctacg atgacatca aagccaaaa 
      ttgccgaag acaacaaag cactgtagt cacaacttt 
      cagattatt tattggaat tttccgctt tatataatt 
      aaaggactg atataagct ttgttacgt tgtttctat 
      gtctaaagt tttaataaa gggacagtt ttaatactt 
      ac