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Gene information
General information
TC004656 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 768393..769607
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004656
    iBeetle screen iB_00745
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004656 RA
      MKLLTTILL LLPFALTGP VEPVRRYVV EENNPEFKR 
      YIYVENKNG QFEEIDLLA VDPEEVQFK VVQSDVEYD 
      LYTRKSQDY PDVVTNGNV DQLAKSHFD PKKITVFVA 
      HGWNNNRNS AVNTHIRQT IFTHHDVNL FIVDWSGPA 
      NKFYLTAKN SVVPVGEYV GDFITKIQN AYGLDGSKF 
      VLIGHSLGA HVVGSAGAT AQGKLAHII GLDPAGPLF 
      SLKHPENRL DISDGEFVQ IIHTNGNLL GFASSIGDV 
      DYFPNGGMS QPGCGWDLA GTCAHSKAY LYLAEALSR 
      GPVFQGVLC SGYKDFQKG RCADNPKAV MGQLSIPTS 
      NLGDYYLDT NSDQPFGRH
    CDS sequence
    • >TC004656 RA
      atgaaactt ctcacaact atccttctt ttgctccct 
      ttcgccctc acaggccct gttgagccg gtcagacga 
      tacgtagtc gaggaaaat aacccggag ttcaaacga 
      tacatttac gttgaaaat aagaatggc cagtttgaa 
      gaaatcgac ttgctggcc gttgatcct gaagaagtc 
      caattcaaa gtggtccaa tcggacgtc gagtatgac 
      ctatacacg cgaaaaagc caagactac cctgacgta 
      gtcacaaac ggaaatgtg gaccaactt gcgaagtcc 
      catttcgac cccaaaaag atcaccgtt ttcgtggcc 
      cacggctgg aacaacaat cgcaattcc gcggtcaac 
      acccacatc cgacagaca attttcacc catcacgac 
      gtcaattta ttcatagtt gactggagc ggccccgcg 
      aacaaattc tacctcact gccaaaaac tccgtagtt 
      ccggttggc gaatacgtc ggtgatttc atcacaaaa 
      atccaaaac gcgtacggc ttggacggc tccaaattc 
      gtcctgatt gggcacagt cttggggca cacgtggtt 
      ggcagtgcg ggggccacg gcccaggga aaactcgcc 
      catatcata ggacttgac cccgcggga cccctcttc 
      tcgctcaaa caccccgaa aatcgcttg gacatctcg 
      gacggagag ttcgtccaa attatccac accaatggc 
      aatttgctc ggttttgcc agctcgatc ggggatgtt 
      gattatttc cccaacggg ggcatgtcc cagcctggg 
      tgtgggtgg gatctggcc ggcacttgt gcccacagc 
      aaggcctat ttgtacttg gccgaggct ttgagccga 
      ggcccggtt ttccaagga gtcctttgc tcgggttat 
      aaggacttc caaaaggga cgatgcgcc gataatccc 
      aaggctgtc atgggacaa cttagtatc ccaacaagc 
      aatctcggc gattactat ttggacacc aactcagac 
      caacccttc ggacgacat taa
    mRNA sequence
    • >TC004656 RA
      gagtcaaaa tgaaacttc tcacaacta tccttcttt 
      tgctccctt tcgccctca caggccctg ttgagccgg 
      tcagacgat acgtagtcg aggaaaata acccggagt 
      tcaaacgat acatttacg ttgaaaata agaatggcc 
      agtttgaag aaatcgact tgctggccg ttgatcctg 
      aagaagtcc aattcaaag tggtccaat cggacgtcg 
      agtatgacc tatacacgc gaaaaagcc aagactacc 
      ctgacgtag tcacaaacg gaaatgtgg accaacttg 
      cgaagtccc atttcgacc ccaaaaaga tcaccgttt 
      tcgtggccc acggctgga acaacaatc gcaattccg 
      cggtcaaca cccacatcc gacagacaa ttttcaccc 
      atcacgacg tcaatttat tcatagttg actggagcg 
      gccccgcga acaaattct acctcactg ccaaaaact 
      ccgtagttc cggttggcg aatacgtcg gtgatttca 
      tcacaaaaa tccaaaacg cgtacggct tggacggct 
      ccaaattcg tcctgattg ggcacagtc ttggggcac 
      acgtggttg gcagtgcgg gggccacgg cccagggaa 
      aactcgccc atatcatag gacttgacc ccgcgggac 
      ccctcttct cgctcaaac accccgaaa atcgcttgg 
      acatctcgg acggagagt tcgtccaaa ttatccaca 
      ccaatggca atttgctcg gttttgcca gctcgatcg 
      gggatgttg attatttcc ccaacgggg gcatgtccc 
      agcctgggt gtgggtggg atctggccg gcacttgtg 
      cccacagca aggcctatt tgtacttgg ccgaggctt 
      tgagccgag gcccggttt tccaaggag tcctttgct 
      cgggttata aggacttcc aaaagggac gatgcgccg 
      ataatccca aggctgtca tgggacaac ttagtatcc 
      caacaagca atctcggcg attactatt tggacacca 
      actcagacc aacccttcg gacgacatt aatttagct 
      aatgctaca ctttatatt gttgtattt gttcaaaaa 
      tacattatt tctaagtaa