Status
Please wait ...
Gene information
General information
TC031516
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004645
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4414045..4420254
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031516
    iBeetle screen iB_00744

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031516 RA
      MGHVVYLLV AISGIVAAD STTTEYKQD KEPVPSKPR 
      DLTVLEVSS DTIMLRWTQ PEKLNGAIE GYRVYYMYN 
      NYTDTNSRI KPVSPYITY NLTNLKPFT EYKIWVKAF 
      TRNHEGEPS DPIYNTTDI SGPSSPKLL NLTCQTQDT 
      IFFQWARPE HFYYSIDYY YIYIYLNDR IWKNISIES 
      STEHLETSY SVENVTTNT EYQVQMQAS TRSNRTKRI 
      VRGPLSEPK TVYMRSDCD KMQEYMRHT THELSAGVL 
      AGVICASFS FLLAVSAFI LWRKCFRVS YYYLDDPPC 
      SVPTASLDW NAPPDGNSE HKGAIPVHL FPKHVAELH 
      ADGDIGFSK EYEAIQAEA TTDEHTSEH SQHPDNRVK 
      NRYLNIIAY DHSRVQLLQ MPGQKKNID YINANYIDG 
      FQWSKAYIG TQGPLPSTF DCFWRMVWE QRVNIIVMI 
      TNLVERGRR KCDMYWPKE GTETYGVIQ VKLVKEDIM 
      ATYTVRTLQ IKHLRIKKK KAAMAEKLV YQYHYTNWP 
      DHGTPDHPL PVLHFVKKS AAANPPDAG PIIVHCSAG 
      VGRTGTYIV LDAMLRQIR SRGEVNIFG FLRHIRSQR 
      NFLVQTEEQ YIFIHDALV EAIESGETN ISREQFPRY 
      VQMLQNLNT EEKSQLWKP IDVQFKLVT SFQCKDFNL 
      VSANKVINQ PKNRTANIL PVESSRVHL TPKPGEDGS 
      DYVNATWMQ GFHSLREFI ITQHPLKTT IKDFWQMVW 
      DHNAQTIVM LSIIDNQEF EVFWPMDQE TVDADSYKV 
      KLVNEQAQS TYVTRDFTM QSLQDDYEI PVKMIHCTN 
      WPHHCCSIA EIYHLPNTV LDMGIQNGP VVVVDRFGG 
      TEAASFCEL TTLKKHLMY DNHVDVYMY AKLYHNKRP 
      GIWGSCDDY LRLHLAVQT LCSPPEGTP DLYAMTNGA 
      LNGSLSNDC VRVPPEEAP LISQDVMKC N
    • >TC031516 RB
      MGHVVYLLV AISGIVAAD STTTEYKQD KEPVPSKPR 
      DLTVLEVSS DTIMLRWTQ PEKLNGAIE GYRVYYMYN 
      NYTDTNSRI KPVSPYITY NLTNLKPFT EYKIWVKAF 
      TRNHEGEPS DPIYNTTDI SGPSSPKLL NLTCQTQDT 
      IFFQWARPE HFYYSIDYY YIYIYLNDR IWKNISIES 
      STEHLETSY SVENVTTNT EYQVQMQAS TRSNRTKRI 
      VRGPLSEPK TVYMRSDCD KMQEYMRHT THELSAGVL 
      AGVICASFS FLLAVSAFI LWRKCFRVS YYYLDDPPC 
      SVPTASLDW NAPPDGNSE HKGAIPVHL FPKHVAELH 
      ADGDIGFSK EYEAIQAEA TTDEHTSEH SQHPDNRVK 
      NRYLNIIAY DHSRVQLLQ MPGQKKNID YINANYIDG 
      FQWSKAYIG TQGPLPSTF DCFWRMVWE QRVNIIVMI 
      TNLVERGRR KCDMYWPKE GTETYGVIQ VKLVKEDIM 
      ATYTVRTLQ IKHLRIKKK KAAMAEKLV YQYHYTNWP 
      DHGTPDHPL PVLHFVKKS AAANPPDAG PIIVHCSAG 
      VGRTGTYIV LDAMLRQIR SRGEVNIFG FLRHIRSQR 
      NFLVQTEEQ YIFIHDALV EAIESGETN ISREQFPRY 
      VQMLQNLNT EEKSQLWKP IDVQFKLVT SFQCKDFNL 
      VSANKVINQ PKNRTANIL PVESSRVHL TPKPGEDGS 
      DYVNATWMQ GFHSLREFI ITQHPLKTT IKDFWQMVW 
      DHNAQTIVM LSIIDNQEF EVFWPMDQE TVDADSYKV 
      KLVNEQAQS TYVTRDFTM QSLQDDYEI PVKMIHCTN 
      WPHHCCSIA EIYHLPNTV LDMGIQNGP VVVVDRFGG 
      TEAASFCEL TTLKKHLMY DNHVDVYMY AKLYHNKRP 
      GIWGSCDDY LRLHLAVQT LCSPPEGTP DLYAMTNGA 
      LNGSLSNDC VRVPPEGME AVGPRLTVA
    CDS sequence
    • >TC031516 RA
      atgggtcat gttgtttat ttactggtc gcgatttcc 
      ggaatcgtc gccgctgac tcgaccact acggaatat 
      aaacaagac aaagaacca gtacctagc aagccgcga 
      gatctcacc gttttggaa gtctcctct gataccatc 
      atgctcaga tggacccaa cctgagaaa ctcaatggg 
      gccatagaa ggatacaga gtttattac atgtataac 
      aactatact gacacgaac agtagaatc aaacctgta 
      tcgccttat atcacatac aacttaacc aacttaaag 
      ccgtttact gaatacaaa atatgggtt aaggcattt 
      actagaaac cacgaggga gaaccatct gatcctatt 
      tacaacaca accgatatt agcggccca agctcgcca 
      aaattattg aacttaact tgccaaaca caagatacg 
      atatttttc cagtgggcc agaccagag catttttat 
      tattctatt gattattat tatatttat atctattta 
      aatgaccgc atttggaag aacatctct attgaatcc 
      tccacggag cacctagag acctcgtac tcagtggag 
      aacgtgacg acaaacacc gagtaccag gtccagatg 
      caggcctcc acgaggagc aaccgcact aagcgcata 
      gtgaggggc cctttatcg gagcccaag acggtctac 
      atgcgttcg gactgcgat aaaatgcag gaatacatg 
      cgtcacacc actcacgag ttgagtgcc ggcgttttg 
      gccggtgtg atttgcgcc agcttctcg tttttattg 
      gccgtatca gcttttatc ctctggaga aaatgcttt 
      cgcgtctcg tactattat ctggacgac cccccgtgt 
      tcggtgccg acggcttcg ctggactgg aatgcgccc 
      cctgacggg aacagcgag cataaaggg gccattccg 
      gtgcacctc ttcccgaaa catgtggcg gagctgcat 
      gctgatggg gatatcggg ttcagtaaa gagtatgag 
      gccattcaa gctgaggcc acgactgat gaacacact 
      tcggaacat tcacagcat cccgacaat cgtgttaaa 
      aatagatat ctcaacata attgcttat gatcatagt 
      agagttcag ctgctccaa atgccagga cagaagaaa 
      aatatcgat tatattaat gccaattac attgatggg 
      tttcaatgg tccaaggcc tacattggc acgcaaggg 
      ccgctgcca tcgaccttc gattgtttc tggagaatg 
      gtgtgggag cagagagtt aatattatc gttatgatt 
      actaattta gtggaaagg ggaagaaga aaatgtgat 
      atgtattgg cccaaagaa ggaacagaa acatacggt 
      gttatccaa gttaagcta gtcaaagaa gacattatg 
      gcaacttac acagtgaga acgttgcaa attaaacac 
      cttaggatt aagaaaaag aaagcagca atggcggag 
      aagttagtg tatcagtac cactatact aactggcct 
      gaccatggc acacccgat catccactc cctgtgtta 
      cattttgtt aaaaaatca gccgcagcc aatcctcct 
      gacgcagga ccaattata gttcattgc agtgctggt 
      gttggtaga actggcact tatatagtt ctagacgca 
      atgctcaga caaattcgt agtcggggt gaagtgaac 
      attttcggt ttcttgcgc cacatccgc tcccaaaga 
      aatttcttg gtccagact gaagaacag tacatattt 
      atccacgat gcgctagtt gaagcaatt gagtcagga 
      gaaacaaat ataagccgc gaacagttc ccccgttat 
      gttcaaatg ctgcaaaac ctaaacact gaagagaaa 
      agtcagttg tggaaacca attgacgta caatttaaa 
      ttagtcacg agttttcaa tgtaaagac ttcaatctc 
      gtttcggca aataaagtg ataaatcag ccaaagaat 
      aggacggca aatattttg ccggtggaa agttcccgt 
      gtacattta actccgaaa ccgggcgaa gatggctct 
      gattatgtt aacgcaact tggatgcaa gggttccac 
      agtttgcgt gaattcata ataacgcaa catccactc 
      aagactaca attaaagac ttttggcaa atggtctgg 
      gaccataac gcacaaact attgtgatg cttagcatt 
      attgataat caagaattt gaagtattc tggcctatg 
      gatcaagaa acggtggat gcggacagc tacaaagtt 
      aagttagtt aacgaacaa gcgcaatcg acttatgtg 
      acaagagac ttcacgatg cagtcgctc caagacgat 
      tatgaaata cccgttaag atgattcat tgcactaat 
      tggccacac cactgctgc agtattgcc gaaatttac 
      catctgcca aatacagtc ctggatatg ggcatccag 
      aacgggcct gttgtcgta gtagacagg tttggcggt 
      acggaggcg gcgtctttt tgtgagttg acaacgctg 
      aagaaacac ttgatgtat gataatcac gtggacgtg 
      tacatgtac gcgaagttg tatcacaat aagaggcct 
      ggtatctgg ggttcctgt gatgattat ttgaggctc 
      catttggca gttcagacg ctgtgtagt ccgccggag 
      ggcacgccc gacctgtac gcgatgaca aatggggcg 
      ctgaatggg tctttatca aatgactgt gtgagagta 
      cctcctgaa gaagctcct ctaatatcc caggatgtt 
      atgaagtgt aactga
    • >TC031516 RB
      atgggtcat gttgtttat ttactggtc gcgatttcc 
      ggaatcgtc gccgctgac tcgaccact acggaatat 
      aaacaagac aaagaacca gtacctagc aagccgcga 
      gatctcacc gttttggaa gtctcctct gataccatc 
      atgctcaga tggacccaa cctgagaaa ctcaatggg 
      gccatagaa ggatacaga gtttattac atgtataac 
      aactatact gacacgaac agtagaatc aaacctgta 
      tcgccttat atcacatac aacttaacc aacttaaag 
      ccgtttact gaatacaaa atatgggtt aaggcattt 
      actagaaac cacgaggga gaaccatct gatcctatt 
      tacaacaca accgatatt agcggccca agctcgcca 
      aaattattg aacttaact tgccaaaca caagatacg 
      atatttttc cagtgggcc agaccagag catttttat 
      tattctatt gattattat tatatttat atctattta 
      aatgaccgc atttggaag aacatctct attgaatcc 
      tccacggag cacctagag acctcgtac tcagtggag 
      aacgtgacg acaaacacc gagtaccag gtccagatg 
      caggcctcc acgaggagc aaccgcact aagcgcata 
      gtgaggggc cctttatcg gagcccaag acggtctac 
      atgcgttcg gactgcgat aaaatgcag gaatacatg 
      cgtcacacc actcacgag ttgagtgcc ggcgttttg 
      gccggtgtg atttgcgcc agcttctcg tttttattg 
      gccgtatca gcttttatc ctctggaga aaatgcttt 
      cgcgtctcg tactattat ctggacgac cccccgtgt 
      tcggtgccg acggcttcg ctggactgg aatgcgccc 
      cctgacggg aacagcgag cataaaggg gccattccg 
      gtgcacctc ttcccgaaa catgtggcg gagctgcat 
      gctgatggg gatatcggg ttcagtaaa gagtatgag 
      gccattcaa gctgaggcc acgactgat gaacacact 
      tcggaacat tcacagcat cccgacaat cgtgttaaa 
      aatagatat ctcaacata attgcttat gatcatagt 
      agagttcag ctgctccaa atgccagga cagaagaaa 
      aatatcgat tatattaat gccaattac attgatggg 
      tttcaatgg tccaaggcc tacattggc acgcaaggg 
      ccgctgcca tcgaccttc gattgtttc tggagaatg 
      gtgtgggag cagagagtt aatattatc gttatgatt 
      actaattta gtggaaagg ggaagaaga aaatgtgat 
      atgtattgg cccaaagaa ggaacagaa acatacggt 
      gttatccaa gttaagcta gtcaaagaa gacattatg 
      gcaacttac acagtgaga acgttgcaa attaaacac 
      cttaggatt aagaaaaag aaagcagca atggcggag 
      aagttagtg tatcagtac cactatact aactggcct 
      gaccatggc acacccgat catccactc cctgtgtta 
      cattttgtt aaaaaatca gccgcagcc aatcctcct 
      gacgcagga ccaattata gttcattgc agtgctggt 
      gttggtaga actggcact tatatagtt ctagacgca 
      atgctcaga caaattcgt agtcggggt gaagtgaac 
      attttcggt ttcttgcgc cacatccgc tcccaaaga 
      aatttcttg gtccagact gaagaacag tacatattt 
      atccacgat gcgctagtt gaagcaatt gagtcagga 
      gaaacaaat ataagccgc gaacagttc ccccgttat 
      gttcaaatg ctgcaaaac ctaaacact gaagagaaa 
      agtcagttg tggaaacca attgacgta caatttaaa 
      ttagtcacg agttttcaa tgtaaagac ttcaatctc 
      gtttcggca aataaagtg ataaatcag ccaaagaat 
      aggacggca aatattttg ccggtggaa agttcccgt 
      gtacattta actccgaaa ccgggcgaa gatggctct 
      gattatgtt aacgcaact tggatgcaa gggttccac 
      agtttgcgt gaattcata ataacgcaa catccactc 
      aagactaca attaaagac ttttggcaa atggtctgg 
      gaccataac gcacaaact attgtgatg cttagcatt 
      attgataat caagaattt gaagtattc tggcctatg 
      gatcaagaa acggtggat gcggacagc tacaaagtt 
      aagttagtt aacgaacaa gcgcaatcg acttatgtg 
      acaagagac ttcacgatg cagtcgctc caagacgat 
      tatgaaata cccgttaag atgattcat tgcactaat 
      tggccacac cactgctgc agtattgcc gaaatttac 
      catctgcca aatacagtc ctggatatg ggcatccag 
      aacgggcct gttgtcgta gtagacagg tttggcggt 
      acggaggcg gcgtctttt tgtgagttg acaacgctg 
      aagaaacac ttgatgtat gataatcac gtggacgtg 
      tacatgtac gcgaagttg tatcacaat aagaggcct 
      ggtatctgg ggttcctgt gatgattat ttgaggctc 
      catttggca gttcagacg ctgtgtagt ccgccggag 
      ggcacgccc gacctgtac gcgatgaca aatggggcg 
      ctgaatggg tctttatca aatgactgt gtgagagta 
      cctcctgaa ggtatggaa gcagtgggg cctcggctc 
      accgttgct taa
    mRNA sequence
    • >TC031516 RA
      actccggtc ccccggtca tgtcggtct ctcgccctt 
      agctcctct ctcatcccc gctcgttgt ttgccgtgc 
      tgtcactgt tctgggtgg tgctggttc ttgcaattt 
      cgggctcta ccatcacgg aatgacccg ttcgacgca 
      gatctgtcg ctggttcgc gctttcgtc tcgtttgta 
      aacatgtcg agcaatgga aactttaca acggcctat 
      tcgcgtaat taatactct tgagtgaag gcttcattg 
      tgttaggat gggtcatgt tgtttattt actggtcgc 
      gatttccgg aatcgtcgc cgctgactc gaccactac 
      ggaatataa acaagacaa agaaccagt acctagcaa 
      gccgcgaga tctcaccgt tttggaagt ctcctctga 
      taccatcat gctcagatg gacccaacc tgagaaact 
      caatggggc catagaagg atacagagt ttattacat 
      gtataacaa ctatactga cacgaacag tagaatcaa 
      acctgtatc gccttatat cacatacaa cttaaccaa 
      cttaaagcc gtttactga atacaaaat atgggttaa 
      ggcatttac tagaaacca cgagggaga accatctga 
      tcctattta caacacaac cgatattag cggcccaag 
      ctcgccaaa attattgaa cttaacttg ccaaacaca 
      agatacgat atttttcca gtgggccag accagagca 
      tttttatta ttctattga ttattatta tatttatat 
      ctatttaaa tgaccgcat ttggaagaa catctctat 
      tgaatcctc cacggagca cctagagac ctcgtactc 
      agtggagaa cgtgacgac aaacaccga gtaccaggt 
      ccagatgca ggcctccac gaggagcaa ccgcactaa 
      gcgcatagt gaggggccc tttatcgga gcccaagac 
      ggtctacat gcgttcgga ctgcgataa aatgcagga 
      atacatgcg tcacaccac tcacgagtt gagtgccgg 
      cgttttggc cggtgtgat ttgcgccag cttctcgtt 
      tttattggc cgtatcagc ttttatcct ctggagaaa 
      atgctttcg cgtctcgta ctattatct ggacgaccc 
      cccgtgttc ggtgccgac ggcttcgct ggactggaa 
      tgcgccccc tgacgggaa cagcgagca taaaggggc 
      cattccggt gcacctctt cccgaaaca tgtggcgga 
      gctgcatgc tgatgggga tatcgggtt cagtaaaga 
      gtatgaggc cattcaagc tgaggccac gactgatga 
      acacacttc ggaacattc acagcatcc cgacaatcg 
      tgttaaaaa tagatatct caacataat tgcttatga 
      tcatagtag agttcagct gctccaaat gccaggaca 
      gaagaaaaa tatcgatta tattaatgc caattacat 
      tgatgggtt tcaatggtc caaggccta cattggcac 
      gcaagggcc gctgccatc gaccttcga ttgtttctg 
      gagaatggt gtgggagca gagagttaa tattatcgt 
      tatgattac taatttagt ggaaagggg aagaagaaa 
      atgtgatat gtattggcc caaagaagg aacagaaac 
      atacggtgt tatccaagt taagctagt caaagaaga 
      cattatggc aacttacac agtgagaac gttgcaaat 
      taaacacct taggattaa gaaaaagaa agcagcaat 
      ggcggagaa gttagtgta tcagtacca ctatactaa 
      ctggcctga ccatggcac acccgatca tccactccc 
      tgtgttaca ttttgttaa aaaatcagc cgcagccaa 
      tcctcctga cgcaggacc aattatagt tcattgcag 
      tgctggtgt tggtagaac tggcactta tatagttct 
      agacgcaat gctcagaca aattcgtag tcggggtga 
      agtgaacat tttcggttt cttgcgcca catccgctc 
      ccaaagaaa tttcttggt ccagactga agaacagta 
      catatttat ccacgatgc gctagttga agcaattga 
      gtcaggaga aacaaatat aagccgcga acagttccc 
      ccgttatgt tcaaatgct gcaaaacct aaacactga 
      agagaaaag tcagttgtg gaaaccaat tgacgtaca 
      atttaaatt agtcacgag ttttcaatg taaagactt 
      caatctcgt ttcggcaaa taaagtgat aaatcagcc 
      aaagaatag gacggcaaa tattttgcc ggtggaaag 
      ttcccgtgt acatttaac tccgaaacc gggcgaaga 
      tggctctga ttatgttaa cgcaacttg gatgcaagg 
      gttccacag tttgcgtga attcataat aacgcaaca 
      tccactcaa gactacaat taaagactt ttggcaaat 
      ggtctggga ccataacgc acaaactat tgtgatgct 
      tagcattat tgataatca agaatttga agtattctg 
      gcctatgga tcaagaaac ggtggatgc ggacagcta 
      caaagttaa gttagttaa cgaacaagc gcaatcgac 
      ttatgtgac aagagactt cacgatgca gtcgctcca 
      agacgatta tgaaatacc cgttaagat gattcattg 
      cactaattg gccacacca ctgctgcag tattgccga 
      aatttacca tctgccaaa tacagtcct ggatatggg 
      catccagaa cgggcctgt tgtcgtagt agacaggtt 
      tggcggtac ggaggcggc gtctttttg tgagttgac 
      aacgctgaa gaaacactt gatgtatga taatcacgt 
      ggacgtgta catgtacgc gaagttgta tcacaataa 
      gaggcctgg tatctgggg ttcctgtga tgattattt 
      gaggctcca tttggcagt tcagacgct gtgtagtcc 
      gccggaggg cacgcccga cctgtacgc gatgacaaa 
      tggggcgct gaatgggtc tttatcaaa tgactgtgt 
      gagagtacc tcctgaaga agctcctct aatatccca 
      ggatgttat gaagtgtaa ctgataatg atttcactt 
      gcgttgcat gttttgtta ttgtaagtg gacaatagt 
      gcttcagag gaaaggtac taagttctc agttgatgt 
      tgatagagc caagtgaaa tgatgtgat gcactatac 
      cgactattc gtttattac aattatttg cctccaata 
      gactcaaga tgtataaaa ttgtagtta tgtgtaaaa 
      agatatgcg tgaacatga atcagatat ttatataat 
      atgtgcagt gtagaataa ttcagtatt tatgtaagt 
      gtatagact tagtttttg tatgatatt gatttgtaa 
      gaagagtac atcgatcat ataaaacat tctgacttg 
      ttctggacc aatttagtg attaatata aattctcat 
      ggtccttca agacatgac tgctcacaa ggccttgaa 
      aggtgtcta ctgacattg gcatggctc ttctcattt 
      tctactgta gaaatggct tattgaata gaatagtat 
      ccgtccacc aggcgatgt atcactcaa cccaactca 
      ctagacacc tttcaagcc attatatca tatattgga 
      tgccgcaat ctcattagg aagtgttca attttaatt 
      aaatttcaa cttgttaaa atactcttt tttacattt 
      ttaaatcat attattttt gcaaattat gcgacaagt 
      tgattttta attgtaggc gagattatt aaaaggagt 
      gtcaatatt tacttcatc gttttttaa accaaattt 
      attttattt taactgtat tgagtctaa tatttatga 
      tgatcaaac tgtagtact ctttaaaaa aagtttttc 
      atcaccttg tgtatgttg cattatatt tgtgttaaa 
      taaaagaaa tttttaata
    • >TC031516 RB
      actccggtc ccccggtca tgtcggtct ctcgccctt 
      agctcctct ctcatcccc gctcgttgt ttgccgtgc 
      tgtcactgt tctgggtgg tgctggttc ttgcaattt 
      cgggctcta ccatcacgg aatgacccg ttcgacgca 
      gatctgtcg ctggttcgc gctttcgtc tcgtttgta 
      aacatgtcg agcaatgga aactttaca acggcctat 
      tcgcgtaat taatactct tgagtgaag gcttcattg 
      tgttaggat gggtcatgt tgtttattt actggtcgc 
      gatttccgg aatcgtcgc cgctgactc gaccactac 
      ggaatataa acaagacaa agaaccagt acctagcaa 
      gccgcgaga tctcaccgt tttggaagt ctcctctga 
      taccatcat gctcagatg gacccaacc tgagaaact 
      caatggggc catagaagg atacagagt ttattacat 
      gtataacaa ctatactga cacgaacag tagaatcaa 
      acctgtatc gccttatat cacatacaa cttaaccaa 
      cttaaagcc gtttactga atacaaaat atgggttaa 
      ggcatttac tagaaacca cgagggaga accatctga 
      tcctattta caacacaac cgatattag cggcccaag 
      ctcgccaaa attattgaa cttaacttg ccaaacaca 
      agatacgat atttttcca gtgggccag accagagca 
      tttttatta ttctattga ttattatta tatttatat 
      ctatttaaa tgaccgcat ttggaagaa catctctat 
      tgaatcctc cacggagca cctagagac ctcgtactc 
      agtggagaa cgtgacgac aaacaccga gtaccaggt 
      ccagatgca ggcctccac gaggagcaa ccgcactaa 
      gcgcatagt gaggggccc tttatcgga gcccaagac 
      ggtctacat gcgttcgga ctgcgataa aatgcagga 
      atacatgcg tcacaccac tcacgagtt gagtgccgg 
      cgttttggc cggtgtgat ttgcgccag cttctcgtt 
      tttattggc cgtatcagc ttttatcct ctggagaaa 
      atgctttcg cgtctcgta ctattatct ggacgaccc 
      cccgtgttc ggtgccgac ggcttcgct ggactggaa 
      tgcgccccc tgacgggaa cagcgagca taaaggggc 
      cattccggt gcacctctt cccgaaaca tgtggcgga 
      gctgcatgc tgatgggga tatcgggtt cagtaaaga 
      gtatgaggc cattcaagc tgaggccac gactgatga 
      acacacttc ggaacattc acagcatcc cgacaatcg 
      tgttaaaaa tagatatct caacataat tgcttatga 
      tcatagtag agttcagct gctccaaat gccaggaca 
      gaagaaaaa tatcgatta tattaatgc caattacat 
      tgatgggtt tcaatggtc caaggccta cattggcac 
      gcaagggcc gctgccatc gaccttcga ttgtttctg 
      gagaatggt gtgggagca gagagttaa tattatcgt 
      tatgattac taatttagt ggaaagggg aagaagaaa 
      atgtgatat gtattggcc caaagaagg aacagaaac 
      atacggtgt tatccaagt taagctagt caaagaaga 
      cattatggc aacttacac agtgagaac gttgcaaat 
      taaacacct taggattaa gaaaaagaa agcagcaat 
      ggcggagaa gttagtgta tcagtacca ctatactaa 
      ctggcctga ccatggcac acccgatca tccactccc 
      tgtgttaca ttttgttaa aaaatcagc cgcagccaa 
      tcctcctga cgcaggacc aattatagt tcattgcag 
      tgctggtgt tggtagaac tggcactta tatagttct 
      agacgcaat gctcagaca aattcgtag tcggggtga 
      agtgaacat tttcggttt cttgcgcca catccgctc 
      ccaaagaaa tttcttggt ccagactga agaacagta 
      catatttat ccacgatgc gctagttga agcaattga 
      gtcaggaga aacaaatat aagccgcga acagttccc 
      ccgttatgt tcaaatgct gcaaaacct aaacactga 
      agagaaaag tcagttgtg gaaaccaat tgacgtaca 
      atttaaatt agtcacgag ttttcaatg taaagactt 
      caatctcgt ttcggcaaa taaagtgat aaatcagcc 
      aaagaatag gacggcaaa tattttgcc ggtggaaag 
      ttcccgtgt acatttaac tccgaaacc gggcgaaga 
      tggctctga ttatgttaa cgcaacttg gatgcaagg 
      gttccacag tttgcgtga attcataat aacgcaaca 
      tccactcaa gactacaat taaagactt ttggcaaat 
      ggtctggga ccataacgc acaaactat tgtgatgct 
      tagcattat tgataatca agaatttga agtattctg 
      gcctatgga tcaagaaac ggtggatgc ggacagcta 
      caaagttaa gttagttaa cgaacaagc gcaatcgac 
      ttatgtgac aagagactt cacgatgca gtcgctcca 
      agacgatta tgaaatacc cgttaagat gattcattg 
      cactaattg gccacacca ctgctgcag tattgccga 
      aatttacca tctgccaaa tacagtcct ggatatggg 
      catccagaa cgggcctgt tgtcgtagt agacaggtt 
      tggcggtac ggaggcggc gtctttttg tgagttgac 
      aacgctgaa gaaacactt gatgtatga taatcacgt 
      ggacgtgta catgtacgc gaagttgta tcacaataa 
      gaggcctgg tatctgggg ttcctgtga tgattattt 
      gaggctcca tttggcagt tcagacgct gtgtagtcc 
      gccggaggg cacgcccga cctgtacgc gatgacaaa 
      tggggcgct gaatgggtc tttatcaaa tgactgtgt 
      gagagtacc tcctgaagg tatggaagc agtggggcc 
      tcggctcac cgttgctta aatttgcag ttatagtgt 
      aaatatatg tgattatcg taattgtta ttatgtcca 
      tatttgtgc cactggtgg caggtgtaa ctgaaaatc 
      atgattaaa acgtacttc tcttgtcta gctcaatca 
      tttttttat atttaacta taggttttt gaagtaatg 
      agagagaag tatggattg atgatccca atgttactt 
      gctgactca ctgtcattc acctatcat cctcagtgt 
      aagtcaata gcatcacat agttatgtt tgttagact 
      gatcaagta ggtatagac atgtattat gccgttaag 
      atcaactcc aaatttcat gttggacat catagttag 
      tcattacca atctgtagt gccacgctt agcttgcta 
      ttgtctcac tgtgcagtt tcctttctt accgatttt 
      tttcacttt taaatgggt ctttgttta cttacagaa 
      gctcctcta atatcccag gatgttatg aagtgtaac 
      tgataatga tttcacttg cgttgcatg ttttgttat 
      tgtaagtgg acaatagtg cttcagagg aaaggtact 
      aagttctca gttgatgtt gatagagcc aagtgaaat 
      gatgtgatg cactatacc gactattcg tttattaca 
      attatttgc ctccaatag actcaagat gtataaaat 
      tgtagttat gtgtaaaaa gatatgcgt gaacatgaa 
      tcagatatt tatataata tgtgcagtg tagaataat 
      tcagtattt atgtaagtg tatagactt agtttttgt 
      atgatattg atttgtaag aagagtaca tcgatcata 
      taaaacatt ctgacttgt tctggacca atttagtga 
      ttaatataa attctcatg gtccttcaa gacatgact 
      gctcacaag gccttgaaa ggtgtctac tgacattgg 
      catggctct tctcatttt ctactgtag aaatggctt 
      attgaatag aatagtatc cgtccacca ggcgatgta 
      tcactcaac ccaactcac tagacacct ttcaagcca 
      ttatatcat atattggat gccgcaatc tcattagga 
      agtgttcaa ttttaatta aatttcaac ttgttaaaa 
      tactctttt ttacatttt taaatcata ttatttttg 
      caaattatg cgacaagtt gatttttaa ttgtaggcg 
      agattatta aaaggagtg tcaatattt acttcatcg 
      ttttttaaa ccaaattta ttttatttt aactgtatt 
      gagtctaat atttatgat gatcaaact gtagtactc 
      tttaaaaaa agtttttca tcaccttgt gtatgttgc 
      attatattt gtgttaaat aaaagaaat ttttaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY