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Gene information
General information
TC004634 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4191621..4196028
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004634
    iBeetle screen iB_00741

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004634 RA
      MLRIAKFCI QNQSKVQNA ALTRFISTT AVQCKTVQN 
      TDKPASPPG ENTSFVMNI FRGQIEGRQ VFPYPNVLT 
      EEQRDTLKM LVDPVSKFF EEVNDPAKN DALEKVDET 
      SLKGLWELG AFGLQVPQD LGGLGLTNT QYARLVEIV 
      GAYDLGVGI TLGAHQSIG FKGILLVGN PEQKAKYLP 
      RVTSGEFAA FCLTEPSSG SDAGSIKTR AVLSPDGKH 
      YILNGSKIW ISNGGLAEI MTVFAQTPV NDPKTGKTV 
      DKVTAFIVE RSFGGVTNG PPEKKMGIK ASNTAEVYY 
      EDVKVPVEN VLGGVGNGF KVAMNILNN GRFGMAAAL 
      SGTMRSCIK KAVEFATQR TQFGQRIDS FGAIQEKIA 
      RMAMLQYVT ESMAYMISG NMDSGSSSY HLEAAISKC 
      FASESAWFV CDEAIQIMG GMGFMKETG LERVLRDLR 
      IFRIFEGTN DILRLFVAL TGIQYAGAH LKELQNAFK 
      NPAANLGLI FGEASKRVV RSVGLASPP AMDHLVHRD 
      LGNAANLLA KKIDAFGQA VEMVLIKYG KSIVNEQFV 
      LNRLANSAI DIYTGAVVL SRASNSLKD HLATAPHEK 
      LMTEAWILE GSERIDNNL KAIATGRNL DNFTKMSKI 
      SKNICEVAG VAQPNPLNL
    CDS sequence
    • >TC004634 RA
      atgttgcga attgcgaag ttttgtatc caaaatcaa 
      agcaaagtc caaaatgcg gcccttaca agattcatc 
      tcaaccact gcggtacag tgcaagact gtgcaaaac 
      acggacaag ccggcctca ccccctggc gagaacacc 
      tccttcgtt atgaacatc ttcaggggg caaattgag 
      gggcgccaa gttttcccc taccccaat gtcctaact 
      gaagagcaa cgcgatact ctaaaaatg ctcgtcgac 
      ccagtttca aaattcttc gaggaggta aacgacccg 
      gcaaaaaat gacgctttg gagaaggtc gacgaaacc 
      tcgctgaag gggctgtgg gagttgggt gcctttgga 
      ctgcaagtc cctcaagat ctgggtggg ttggggctt 
      actaatact cagtatgcc agactggtt gaaatcgtg 
      ggggcgtac gatttgggg gtggggatc acgttaggg 
      gcccaccag tccattggg tttaagggg attttgttg 
      gtggggaac cctgaacaa aaggctaag tatttacca 
      agggtcact tcgggggaa ttcgccgcg ttttgcctg 
      actgaacca tcgtcaggg tcagacgcc ggttcaata 
      aaaactcgc gccgtattg tctcctgac ggcaaacac 
      tacatcctc aacggttcc aaaatctgg ataagtaac 
      ggcgggttg gctgaaata atgacagtt ttcgcccaa 
      acccccgtc aacgaccca aaaacgggc aaaaccgtt 
      gacaaagtc acggctttc atcgtcgaa cgctcgttc 
      gggggtgtc acaaacggg cccccggag aagaaaatg 
      gggatcaaa gcctcaaac accgctgag gtctactac 
      gaagacgtc aaggttcct gtcgaaaac gtccttggc 
      ggtgtgggc aacgggttt aaagtggca atgaacatt 
      ttgaacaac ggccgtttt ggcatggct gctgccttg 
      tccgggacc atgcgctca tgcatcaaa aaagccgta 
      gaattcgca acgcaaaga actcaattc gggcaaaga 
      atcgactct tttggggcc attcaagag aagatcgcc 
      agaatggcc atgttgcag tacgtgact gagagcatg 
      gcgtacatg atttcgggg aacatggat tcggggtcg 
      tccagttac cacctggaa gccgccatt tcaaaatgt 
      tttgcgtcg gagtcggca tggttcgtg tgcgacgag 
      gcgattcag atcatgggt ggcatgggg ttcatgaag 
      gagacgggg cttgagcgc gttttgagg gatttgagg 
      attttccga atttttgag ggaactaac gatattttg 
      cgacttttc gttgccttg actggtatt cagtacgca 
      ggggcgcac ctcaaagaa ttgcaaaat gcgttcaaa 
      aaccccgcc gctaatttg ggcctaatc tttggggag 
      gcctcaaag cgggtcgta aggtctgta ggtttggcg 
      tcgccgcct gccatggac cacttggtg cacagagac 
      ttgggcaat gctgcgaat ttgctcgcc aagaaaatt 
      gacgcgttt ggacaagct gtcgaaatg gttttaatt 
      aagtatggg aagagtatt gtgaacgaa caatttgtt 
      ttgaatagg ttggccaat tcggctatt gatatttac 
      accggagcg gtggttctg tctagggcc agtaattca 
      cttaaggac catctagcc acggcccct cacgagaaa 
      ttaatgact gaagcttgg atattagag ggctcggaa 
      cggatcgat aacaacctg aaagccatt gcaaccgga 
      aggaatttg gacaacttc acgaagatg agcaaaatt 
      tcgaaaaat atctgcgaa gtcgcggga gtggcacaa 
      cccaacccc ttgaacttg taa
    mRNA sequence
    • >TC004634 RA
      tacgttggc gtcgtgact catgggtct catgacggt 
      aataaggac aaatgtacc aaaatagag catatgtag 
      tgttttatt ggcaaaagc aaatttttt gaataaaaa 
      ataaatttt cttcttggt ggaaagtgc gaaaccagg 
      ggtaccaac aggtccggt tgtcgaatt atttttagt 
      ccacctgtt tgcgttgag tattttcgg tccgaagtc 
      caaagttct agtcgagtg tcttaattt ttacttatt 
      aatttccaa atcttcgtt catttcatt gcgtttgta 
      tcgtgggca aggatctat tcaattaca taaagcaca 
      tccgtgata cattttttt aagttctaa gttaagttt 
      tagtggtat tgagtgatt aatttgtag taattatgt 
      tgcgaattg cgaagtttt gtatccaaa atcaaagca 
      aagtccaaa atgcggccc ttacaagat tcatctcaa 
      ccactgcgg tacagtgca agactgtgc aaaacacgg 
      acaagccgg cctcacccc ctggcgaga acacctcct 
      tcgttatga acatcttca gggggcaaa ttgaggggc 
      gccaagttt tcccctacc ccaatgtcc taactgaag 
      agcaacgcg atactctaa aaatgctcg tcgacccag 
      tttcaaaat tcttcgagg aggtaaacg acccggcaa 
      aaaatgacg ctttggaga aggtcgacg aaacctcgc 
      tgaaggggc tgtgggagt tgggtgcct ttggactgc 
      aagtccctc aagatctgg gtgggttgg ggcttacta 
      atactcagt atgccagac tggttgaaa tcgtggggg 
      cgtacgatt tgggggtgg ggatcacgt taggggccc 
      accagtcca ttgggttta aggggattt tgttggtgg 
      ggaaccctg aacaaaagg ctaagtatt taccaaggg 
      tcacttcgg gggaattcg ccgcgtttt gcctgactg 
      aaccatcgt cagggtcag acgccggtt caataaaaa 
      ctcgcgccg tattgtctc ctgacggca aacactaca 
      tcctcaacg gttccaaaa tctggataa gtaacggcg 
      ggttggctg aaataatga cagttttcg cccaaaccc 
      ccgtcaacg acccaaaaa cgggcaaaa ccgttgaca 
      aagtcacgg ctttcatcg tcgaacgct cgttcgggg 
      gtgtcacaa acgggcccc cggagaaga aaatgggga 
      tcaaagcct caaacaccg ctgaggtct actacgaag 
      acgtcaagg ttcctgtcg aaaacgtcc ttggcggtg 
      tgggcaacg ggtttaaag tggcaatga acattttga 
      acaacggcc gttttggca tggctgctg ccttgtccg 
      ggaccatgc gctcatgca tcaaaaaag ccgtagaat 
      tcgcaacgc aaagaactc aattcgggc aaagaatcg 
      actcttttg gggccattc aagagaaga tcgccagaa 
      tggccatgt tgcagtacg tgactgaga gcatggcgt 
      acatgattt cggggaaca tggattcgg ggtcgtcca 
      gttaccacc tggaagccg ccatttcaa aatgttttg 
      cgtcggagt cggcatggt tcgtgtgcg acgaggcga 
      ttcagatca tgggtggca tggggttca tgaaggaga 
      cggggcttg agcgcgttt tgagggatt tgaggattt 
      tccgaattt ttgagggaa ctaacgata ttttgcgac 
      ttttcgttg ccttgactg gtattcagt acgcagggg 
      cgcacctca aagaattgc aaaatgcgt tcaaaaacc 
      ccgccgcta atttgggcc taatctttg gggaggcct 
      caaagcggg tcgtaaggt ctgtaggtt tggcgtcgc 
      cgcctgcca tggaccact tggtgcaca gagacttgg 
      gcaatgctg cgaatttgc tcgccaaga aaattgacg 
      cgtttggac aagctgtcg aaatggttt taattaagt 
      atgggaaga gtattgtga acgaacaat ttgttttga 
      ataggttgg ccaattcgg ctattgata tttacaccg 
      gagcggtgg ttctgtcta gggccagta attcactta 
      aggaccatc tagccacgg cccctcacg agaaattaa 
      tgactgaag cttggatat tagagggct cggaacgga 
      tcgataaca acctgaaag ccattgcaa ccggaagga 
      atttggaca acttcacga agatgagca aaatttcga 
      aaaatatct gcgaagtcg cgggagtgg cacaaccca 
      accccttga acttgtaat ctatttatt ttcttattt 
      gtatattat tgttatgta tgtcccgaa gtgactctt 
      tttacagaa ttcttgtac aaaatcttt tataataaa 
      tttttacac acatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY