| General information | |||
| TC004631 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 4117989..4127483 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004631 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00739 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
Days post injection
adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
Days post injection
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
Days post injection
larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
Days post injection
pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (16 dpi)
Days post injection
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 4 (16 dpi)
Days post injection
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 7 (19 dpi)
Days post injection
- >TC004631 RA
MELSHSLTL NEEALAQIP PAKRPVFIF EWLRFLDKV LVAAQKNDI KGCQKKLVE QLMNHIQES PGPPTRKLI ARSLATLFS VGDTFLLFD TVNKCNDIL KNKDDSPSF LPTRLAAIC CVGTMYEKL GRMMGRSYE ETVQILTRS LRSAESQTR IEIMLTLEK VCAGMGNAI SNVHKDIYK AARHCLIDR VMAVRCAAT RCLLEMLNH APFLYTSEL ESLATLCFR AFDGSNYEV RCAVAKLLG ALIAMTQNQ KPEKTPQLK GLKLVSLDE ALGILMAGF LRGGVGFLK GTGEIIKGS SSVNREVRV GVTHAYVIF VQILGSVWL ERNIKAFLS HILYLVANP KAASSHVDA VYSRKCINF ILRSVLGKM LGEKAQTSA CKEIAQIIV KEMNSIDFN PENAKDFNQ ETLFSQHLL VCALQEIGC LVLSLGTTA HDLITDQTL NLIDATASV LIHPCQAAR LAAAWCLRC ICVAVPSQI TPLIDRCVN GIEEYRTSP EAIAGYSGA LAAVLGGVN LSPLGVPHT KGKIIFNTA EELLRSASQ NSRLSLNRT QAGWLLIGA IMTLGVPVV RGLLPRMLL LWRNSFPRS TKELDSEKA RGDAFTWQV TLEGRAGAL SAMHSFLQH CHELVTDDI NRRLLAPIE SALAMLSNI SSILKSYGQ QLKAPAAMV RLRLYETLL LLPPQSFES SYTHLLRML VAEFTLTEN PANTTTSQM RTVCHADDS VILGTWLQE TDHRTIEDQ LQPNSAAGS GALEHDPCC LYRQISTSE VIPGPLPLG VAVIDMSVL LFGQIFPRV ANKHRLQMV DHFAECIKH AKSSRQEAV QMNVFTALL SGLKGLTEA KMSIGQEDV KKAVTGLII GALTSPNPI LRCAAGEAV GRMAQVVSD PKFTAELAQ TSFDRLKSA RDVASRTGH SLALGCLHR YVGGMGSSQ HLNTSVSIL LALAQDQTS PVVQVWSLH ALALIADSG GPMFRGYVE PSLSLALKL LLNVPQSYI DVHQCIGKV LSALITTIG PELQGNTSS ICMARSSFL CACAIMQDH QDPLVQAEA TGCLQQLHL FAPRHVNLS SLVPTLCRT LSSNHLLLR KAAISCLRQ LAQREAKEV CEHAMTLAN ENRDNNTVE GLVITETGL PGVFFSMLD TETDAALIK DIHDTLISM LQMLAADNL SQWLGLCKD VLTIATDST NMEDQQSVS APDENDEPE ADDDQAEFH AEDDSSTHP AVQPRWPTR VFAAECVRK IITACESSA NKTIHFDLI QAKEMGKSD FLVLHLSEL IRMAFIAAT SDSDPLRLE GLKTLQDII EKFSKVPEP EFPGHLLLE QFQAQVGAA LRPAFAPDT SSHVTAAAC EVCSTWIGS NVARDLNDL RRVHQLLVS SLTKLQNKT NTTQLYNES LATLEKLAI LKAWAEVYI VAMKGNESA NLDVTTANA NDDEFGDFE YKGENLLTL VQPELVSLS QHWLAALKD HALLSLPAE FASQLPHDG GAFYTNDTM ESSRPHYIS SWPPILHAA ALWLNSGGF DKLAEIDND NTNNNTVDT SSDKFHLLF GICMEALCS PRSVEPLES IITCLKALF TLLDSTYAR ELILVDNSL GIELCNVLH RLILTRDNH TCQLLCMEV LKQVIKAAR EKLDRAKKQ STTDSDLLG EGGEAGDIL PGKSLVFSI LEVCLCLLV RQVPSLSPS PNSTIVNSL RIMQTPDES GRLIASSLA CMENLHRLC SPQGALSIL PTILYLTTG VIKEMATKN INDVTVLAN NASVQAALH CLKILTTDR YCNDPRSCD TWQKLLQSA LAKIIDLAK TGSDENKLD EVTMMLAIA VFVLHAPSK VVTAPNLQY PCINHFRQC LQSSNSTVR LKCVQTLKS LFAHPDRKV ATPYIHTLA PRLVEFLYS DAARNIFSD GELFVTLET IVTVESLIE LAEPQNRIQ MLTLLVPIL ISYLLEGQA LRSANKYSL NLHEISLQR LMKIGPKYP QEFKKLMSQ TSELRTKLE NAIRSKQQN HIKIKNGVN ANKPITSHH PSIKLKTDF SNFS
- >TC004631 RB
MELSHSLTL NEEALAQIP PAKRPVFIF EWLRFLDKV LVAAQKNDI KGCQKKLVE QLMNHIQES PGPPTRKLI ARSLATLFS VGDTFLLFD TVNKCNDIL KNKDDSPSF LPTRLAAIC CVGTMYEKL GRMMGRSYE ETVQILTRS LRSAESQTR IEIMLTLEK VCAGMGNAI SNVHKDIYK AARHCLIDR VMAVRCAAT RCLLEMLNH APFLYTSEL ESLATLCFR AFDGSNYEV RCAVAKLLG ALIAMTQNQ KPEKTPQLK GLKLVSLDE ALGILMAGF LRGGVGFLK GTGEIIKGS SSVNREVRV GVTHAYVIF VQILGSVWL ERNIKAFLS HILYLVANP KAASSHVDA VYSRKCINF ILRSVLGKM LGEKAQTSA CKEIAQIIV KEMNSIDFN PENAKDFNQ ETLFSQHLL VCALQEIGC LVLSLGTTA HDLITDQTL NLIDATASV LIHPCQAAR LAAAWCLRC ICVAVPSQI TPLIDRCVN GIEEYRTSP EAIAGYSGA LAAVLGGVN LSPLGVPHT KGKIIFNTA EELLRSASQ NSRLSLNRT QAGWLLIGA IMTLGVPVV RGLLPRMLL LWRNSFPRS TKELDSEKA RGDAFTWQV TLEGRAGAL SAMHSFLQH CHELVTDDI NRRLLAPIE SALAMLSNI SSILKSYGQ QLKAPAAMV RLRLYETLL LLPPQSFES SYTHLLRML VAEFTLTEN PANTTTSQM RTVCHADDS VILGTWLQE TDHRTIEDQ MEPNRRADG EHLQPNSAA GSGALEHDP CCLYRQIST SEVIPGPLP LGVAVIDMS VLLFGQIFP RVANKHRLQ MVDHFAECI KHAKSSRQE AVQMNVFTA LLSGLKGLT EAKMSIGQE DVKKAVTGL IIGALTSPN PILRCAAGE AVGRMAQVV SDPKFTAEL AQTSFDRLK SARDVASRT GHSLALGCL HRYVGGMGS SQHLNTSVS ILLALAQDQ TSPVVQVWS LHALALIAD SGGPMFRGY VEPSLSLAL KLLLNVPQS YIDVHQCIG KVLSALITT IGPELQGNT SSICMARSS FLCACAIMQ DHQDPLVQA EATGCLQQL HLFAPRHVN LSSLVPTLC RTLSSNHLL LRKAAISCL RQLAQREAK EVCEHAMTL ANENRDNNT VEGLVITET GLPGVFFSM LDTETDAAL IKDIHDTLI SMLQMLAAD NLSQWLGLC KDVLTIATD STNMEDQQS VSAPDENDE PEADDDQAE FHAEDDSST HPAVQPRWP TRVFAAECV RKIITACES SANKTIHFD LIQAKEMGK SDFLVLHLS ELIRMAFIA ATSDSDPLR LEGLKTLQD IIEKFSKVP EPEFPGHLL LEQFQAQVG AALRPAFAP DTSSHVTAA ACEVCSTWI GSNVARDLN DLRRVHQLL VSSLTKLQN KTNTTQLYN ESLATLEKL AILKAWAEV YIVAMKGNE SANLDVTTA NANDDEFGD FEYKGENLL TLVQPELVS LSQHWLAAL KDHALLSLP AEFASQLPH DGGAFYTND TMESSRPHY ISSWPPILH AAALWLNSG GFDKLAEID NDNTNNNTV DTSSDKFHL LFGICMEAL CSPRSVEPL ESIITCLKA LFTLLDSTY ARELILVDN SLGIELCNV LHRLILTRD NHTCQLLCM EVLKQVIKA AREKLDRAK KQSTTDSDL LGEGGEAGD ILPGKSLVF SILEVCLCL LVRQVPSLS PSPNSTIVN SLRIMQTPD ESGRLIASS LACMENLHR LCSPQGALS ILPTILYLT TGVIKEMAT KNINDVTVL ANNASVQAA LHCLKILTT DRYCNDPRS CDTWQKLLQ SALAKIIDL AKTGSDENK LDEVTMMLA IAVFVLHAP SKVVTAPNL QYPCINHFR QCLQSSNST VRLKCVQTL KSLFAHPDR KVATPYIHT LAPRLVEFL YSDAARNIF SDGELFVTL ETIVTVESL IELAEPQNR IQMLTLLVP ILISYLLEG QALRSANKY SLNLHEISL QRLMKIGPK YPQEFKKLM SQTSELRTK LENAIRSKQ QNHIKIKNG VNANKPITS HHPSIKLKT DFSNFS
- >TC004631 RA
atggaacta tcgcatagt ttaacgctt aatgaggaa gctttggct caaatcccg ccagcgaag cgacccgtt tttatattc gaatggctt cgattttta gacaaagtt ttagtcgcc gcacagaag aacgacatc aaaggatgt cagaagaaa ttagtagaa caactaatg aaccacatt caagaatct ccgggccct ccaacgcgg aaattaata gcccgctct ctcgcgacc ctcttttca gtcggagac actttcctg ctcttcgac acggtgaac aaatgcaac gatattttg aaaaataaa gacgactct cccagtttc ctcccgacg cgattagct gcgatttgc tgcgtgggg acaatgtac gaaaagctg ggccgaatg atgggccgt tcctacgag gagactgtg caaatttta actcgctcc ctccgcagc gccgagtcc caaactcga atcgaaatc atgttgacg ctcgaaaaa gtgtgtgcc gggatggga aacgctatt tcaaatgtt cataaagat atttacaaa gcggcaaga cattgcctg attgatcgg gtcatggcg gtgagatgt gccgccacc agatgtctt ctcgaaatg ttgaatcat gcccccttt ctttacacc agcgaattg gaaagtctg gcgactttg tgctttcgg gcctttgat ggctcgaat tacgaagtc agatgcgcg gtcgctaaa ctgttagga gcgcttatc gccatgact cagaaccag aaacccgaa aagacaccg cagctcaag gggctgaag ctggtgtcg ttggacgag gctttgggc atcctcatg gcgggtttt ttgcgtggg ggtgtgggt tttttgaaa ggcacagga gagataatt aagggtagt tcgagtgtt aaccgcgaa gtccgagtc ggagtaaca cacgcgtat gtgattttt gtccaaatt ttgggcagt gtgtggctt gagcgtaac ataaaagct ttcctttcg cacatcttg tatttggtc gcgaatccg aaagcggcg tcttcgcac gtcgatgct gtctattcc cggaaatgt ataaatttt attttgagg agtgtgttg gggaaaatg ttgggagag aaagcacaa acttcggcg tgtaaagaa atcgcacag attattgtt aaagaaatg aactcgatc gattttaat cccgagaat gcgaaagac ttcaatcag gagacgctt ttcagccag catttacta gtgtgtgcc ttgcaagaa atcgggtgt ttggttttg agtttaggg acgacggca cacgacttg atcaccgac caaactttg aatttgatc gacgctact gctagtgtt ttgatccat ccgtgccaa gcagcgcgt cttgcagct gcttggtgc ttgcggtgt atttgtgtg gctgttcct agccaaatc acccctttg atagaccgc tgtgttaac ggaatcgag gaatacagg acttcgccg gaagctata gccggctat agtggagct ttagctgcg gttctgggc ggtgttaac ttatccccg cttggagtg ccacacact aaaggcaaa atcattttt aacacagcg gaagagttg ctaagaagt gccagtcaa aatagccgg ctgtcgcta aaccggact caagccggt tggctactt atcggtgct attatgaca ctgggggtt ccagtagtt agggggttg ctccctcgc atgttgctc ctttggagg aattctttc cccaggtct actaaagaa ctagattcg gaaaaagcc cgaggtgat gctttcacc tggcaagtg actctcgaa ggccgcgct ggggccctg tctgctatg cacagcttc ctccaacac tgtcacgag ttagtcact gatgatatt aacagaagg ttgctggcg ccaatcgaa tctgctcta gcaatgcta tcaaacatt tcgagtatt ttgaaaagt tacggacaa cagttgaag gctccggcg gccatggtc cgcttacgc ctctacgaa accctgctg ttgcttccg ccgcaatct ttcgaaagc tcgtacact catcttttg cgaatgttg gtcgcggaa tttactttg accgaaaat ccggcaaac accacaacg tcccagatg cggactgtg tgccatgct gacgattcc gtaatttta ggcacttgg ttacaagaa actgatcat agaacaatc gaggatcaa ttacagccg aacagtgcg gccggttct ggagccctt gaacacgac ccgtgctgt ctctaccgc caaatttca acaagcgaa gtaattccg ggtccactc cctctggga gttgcagtg atcgacatg tctgttctc ctcttcggc cagattttc ccacgtgtg gccaataag caccgcttg caaatggtc gaccatttc gccgaatgc atcaaacac gcgaagagt tcgcgccag gaagcggtg caaatgaac gtatttacg gcccttttg agcggactg aaaggccta accgaggcc aaaatgagc ataggccag gaggacgtc aaaaaagcc gttacgggc ctaattatc ggtgcttta acttcaccg aacccaatt ctgcggtgc gcggcggga gaagccgtc ggacgtatg gctcaagta gtatccgac ccaaaattc accgcagaa ttggctcag acgagcttc gaccgactt aagtcagca cgtgatgta gcaagccga actgggcat tcgttagcc ctcggctgc ttgcaccgc tacgttgga ggtatgggc tccagccaa cacttgaat acgagtgtt tcaattttg ctggcgctt gcgcaagac cagacctcg ccagtggtt caagtgtgg tcgctacat gcacttgca ctaatcgcg gattcgggg ggtcccatg tttcggggt tacgtcgaa ccgagtctt tccctagct ttgaaactt ctactaaat gtaccacag tcgtatatt gacgttcat cagtgtatt gggaaagtt ctctcggcc ttaattacg acaatcggg cccgaattg caaggcaat acgtcgagt atttgtatg gcaaggagt tcttttttg tgtgcttgt gccatcatg caagaccac caagacccg ttggtccaa gcggaagcc acaggatgt ctccaacaa ctgcattta ttcgcaccg agacatgtt aatttatcg tcactagtc ccgacactt tgtcgcact ttatcgagt aatcatctt ttgttacgt aaagctgcc atttcctgt ctaagacaa ttggcacaa agggaggct aaagaagta tgcgaacac gcaatgact ttggccaac gaaaatcgt gataataac accgtggaa ggtttagtg ataaccgaa accggtctt ccgggcgtt ttcttcagt atgttagac actgaaacc gacgctgcc ctaatcaaa gacatccac gatacgtta atcagcatg ctacaaatg ctcgcagct gacaactta agccaatgg ttgggcttg tgcaaggac gtgctaacc atcgcaacc gatagcaca aacatggaa gaccaacaa agcgtctca gctccggac gaaaacgac gaacctgag gcagacgac gaccaagca gagtttcac gctgaggac gactcttcc acccacccc gcggtgcag ccccgatgg ccaacgcgt gtttttgca gccgaatgc gtccgaaaa ataatcaca gcctgcgaa agcagcgca aacaaaacg attcatttc gatctaatc caagccaaa gagatgggg aaaagcgat ttcctcgtc ttgcattta tccgaactg atccgaatg gcgttcatt gccgcgacg agcgactcg gacccgttg cgtctcgaa ggcttgaaa accctccaa gatataatc gagaagttt tccaaagtt ccagaaccg gagttcccc ggccacttg ttactcgag cagttccaa gcgcaagtc ggtgcagcg ctaagacca gctttcgca cccgatact tcctcacat gttacagca gccgcttgc gaagtttgc tcgacttgg atcggctcg aacgtggca cgggattta aatgatttg cgccgcgtg caccaacta ctagtctca tccttgact aagttgcaa aataagacg aacacaacg caactctac aacgagagc ttagcaact ttagagaag ttggcgatt ctgaaggct tgggccgag gtttacatt gttgcaatg aaaggaaat gaaagtgcg aatttggac gtaacaacg gccaatgcc aatgatgac gagtttggc gattttgaa tacaaggga gagaatttg ttgactttg gtgcaaccc gagttggtt agtttgtcg caacattgg ctggccgca ctgaaagac catgcctta ttgtcacta ccggcagaa ttcgccagt cagttgcca cacgacggt ggcgctttt tacaccaat gatacaatg gaatcgtca cgaccgcac tacatatcc tcatggcct ccaatccta catgctgca gctctgtgg ctcaactcc ggcggtttt gataaactt gccgaaata gacaacgat aatacaaac aataacacg gttgatacg tccagcgat aagttccat ctcttgttt ggcatatgc atggaggcg ttgtgcagc ccccgatcg gtcgaaccc ctcgaaagt ataataaca tgtcttaaa gcactgttt actctcctg gactcaaca tacgcccgc gagttgatt ctcgtcgac aactcgctt gggattgaa ttgtgcaac gttttgcac cgcctcatc ctcacacgt gataaccac acgtgccaa ctcttatgc atggaagtc ctcaagcaa gtgatcaaa gcagcgcgc gaaaaactc gaccgcgct aagaaacaa tcaacgacc gattccgat ttactgggc gaaggtggc gaagctggc gacatttta cccggcaaa tcgctcgtt ttctcaatt ttggaagta tgtctgtgt ctgttggtt cgccaggtg ccatcgcta agcccctcg cccaactcg acaatcgtc aattcgctg cgaatcatg caaacgccg gacgaatcc ggccgcttg atcgcctcg tctttggcc tgcatggaa aatttacac aggttgtgt tcgccgcaa ggcgcccta tccatactc ccaacaatc ctgtatttg accaccggg gtgatcaag gaaatggcg acgaaaaat atcaacgac gtgacggtg ctagcgaat aacgcctcg gtccaagcg gccctccac tgtctcaaa atcctcaca acggataga tattgcaac gacccccga agctgtgat acctggcag aagctgctc cagagcgcc ctcgccaaa atcatcgat cttgcgaaa accggcagc gatgagaat aaactcgat gaagtcaca atgatgcta gccatcgcg gttttcgtc ctccatgcg ccttcgaaa gttgtaaca gcccccaat ttgcaatac ccctgcatt aaccacttc cgacaatgc ctccaaagc tcgaattcg acggtaagg ttgaaatgt gttcaaact ttgaaatct ttgtttgct catccggat cggaaagtc gcaactccc tatattcac acactcgct ccgcggctc gtcgaattt ttgtattcc gatgctgcg aggaatatt ttttcagac ggggagctt tttgttacg ttggaaact atcgtaacg gttgaatca ctgatcgag ttggcggaa ccacaaaat cgaattcag atgctcacc cttctggtt ccgatcttg ataagttat ttgttggaa ggtcaggcg cttaggtca gcgaataaa tactcgttg aatttgcac gaaatcagc ttgcaaaga ctgatgaaa atcggacca aaatatccc caggagttt aaaaaatta atgagtcag actagtgag cttagaacg aaattagaa aatgcgatt agatcaaaa caacaaaac cacatcaaa atcaagaac ggagtcaat gcgaataaa cccataacg agccatcac ccttcaatt aaattaaaa accgacttt agtaatttt tcctaa
- >TC004631 RB
atggaacta tcgcatagt ttaacgctt aatgaggaa gctttggct caaatcccg ccagcgaag cgacccgtt tttatattc gaatggctt cgattttta gacaaagtt ttagtcgcc gcacagaag aacgacatc aaaggatgt cagaagaaa ttagtagaa caactaatg aaccacatt caagaatct ccgggccct ccaacgcgg aaattaata gcccgctct ctcgcgacc ctcttttca gtcggagac actttcctg ctcttcgac acggtgaac aaatgcaac gatattttg aaaaataaa gacgactct cccagtttc ctcccgacg cgattagct gcgatttgc tgcgtgggg acaatgtac gaaaagctg ggccgaatg atgggccgt tcctacgag gagactgtg caaatttta actcgctcc ctccgcagc gccgagtcc caaactcga atcgaaatc atgttgacg ctcgaaaaa gtgtgtgcc gggatggga aacgctatt tcaaatgtt cataaagat atttacaaa gcggcaaga cattgcctg attgatcgg gtcatggcg gtgagatgt gccgccacc agatgtctt ctcgaaatg ttgaatcat gcccccttt ctttacacc agcgaattg gaaagtctg gcgactttg tgctttcgg gcctttgat ggctcgaat tacgaagtc agatgcgcg gtcgctaaa ctgttagga gcgcttatc gccatgact cagaaccag aaacccgaa aagacaccg cagctcaag gggctgaag ctggtgtcg ttggacgag gctttgggc atcctcatg gcgggtttt ttgcgtggg ggtgtgggt tttttgaaa ggcacagga gagataatt aagggtagt tcgagtgtt aaccgcgaa gtccgagtc ggagtaaca cacgcgtat gtgattttt gtccaaatt ttgggcagt gtgtggctt gagcgtaac ataaaagct ttcctttcg cacatcttg tatttggtc gcgaatccg aaagcggcg tcttcgcac gtcgatgct gtctattcc cggaaatgt ataaatttt attttgagg agtgtgttg gggaaaatg ttgggagag aaagcacaa acttcggcg tgtaaagaa atcgcacag attattgtt aaagaaatg aactcgatc gattttaat cccgagaat gcgaaagac ttcaatcag gagacgctt ttcagccag catttacta gtgtgtgcc ttgcaagaa atcgggtgt ttggttttg agtttaggg acgacggca cacgacttg atcaccgac caaactttg aatttgatc gacgctact gctagtgtt ttgatccat ccgtgccaa gcagcgcgt cttgcagct gcttggtgc ttgcggtgt atttgtgtg gctgttcct agccaaatc acccctttg atagaccgc tgtgttaac ggaatcgag gaatacagg acttcgccg gaagctata gccggctat agtggagct ttagctgcg gttctgggc ggtgttaac ttatccccg cttggagtg ccacacact aaaggcaaa atcattttt aacacagcg gaagagttg ctaagaagt gccagtcaa aatagccgg ctgtcgcta aaccggact caagccggt tggctactt atcggtgct attatgaca ctgggggtt ccagtagtt agggggttg ctccctcgc atgttgctc ctttggagg aattctttc cccaggtct actaaagaa ctagattcg gaaaaagcc cgaggtgat gctttcacc tggcaagtg actctcgaa ggccgcgct ggggccctg tctgctatg cacagcttc ctccaacac tgtcacgag ttagtcact gatgatatt aacagaagg ttgctggcg ccaatcgaa tctgctcta gcaatgcta tcaaacatt tcgagtatt ttgaaaagt tacggacaa cagttgaag gctccggcg gccatggtc cgcttacgc ctctacgaa accctgctg ttgcttccg ccgcaatct ttcgaaagc tcgtacact catcttttg cgaatgttg gtcgcggaa tttactttg accgaaaat ccggcaaac accacaacg tcccagatg cggactgtg tgccatgct gacgattcc gtaatttta ggcacttgg ttacaagaa actgatcat agaacaatc gaggatcaa atggagcct aaccgaagg gcagatggt gaacattta cagccgaac agtgcggcc ggttctgga gcccttgaa cacgacccg tgctgtctc taccgccaa atttcaaca agcgaagta attccgggt ccactccct ctgggagtt gcagtgatc gacatgtct gttctcctc ttcggccag attttccca cgtgtggcc aataagcac cgcttgcaa atggtcgac catttcgcc gaatgcatc aaacacgcg aagagttcg cgccaggaa gcggtgcaa atgaacgta tttacggcc cttttgagc ggactgaaa ggcctaacc gaggccaaa atgagcata ggccaggag gacgtcaaa aaagccgtt acgggccta attatcggt gctttaact tcaccgaac ccaattctg cggtgcgcg gcgggagaa gccgtcgga cgtatggct caagtagta tccgaccca aaattcacc gcagaattg gctcagacg agcttcgac cgacttaag tcagcacgt gatgtagca agccgaact gggcattcg ttagccctc ggctgcttg caccgctac gttggaggt atgggctcc agccaacac ttgaatacg agtgtttca attttgctg gcgcttgcg caagaccag acctcgcca gtggttcaa gtgtggtcg ctacatgca cttgcacta atcgcggat tcggggggt cccatgttt cggggttac gtcgaaccg agtctttcc ctagctttg aaacttcta ctaaatgta ccacagtcg tatattgac gttcatcag tgtattggg aaagttctc tcggcctta attacgaca atcgggccc gaattgcaa ggcaatacg tcgagtatt tgtatggca aggagttct tttttgtgt gcttgtgcc atcatgcaa gaccaccaa gacccgttg gtccaagcg gaagccaca ggatgtctc caacaactg catttattc gcaccgaga catgttaat ttatcgtca ctagtcccg acactttgt cgcacttta tcgagtaat catcttttg ttacgtaaa gctgccatt tcctgtcta agacaattg gcacaaagg gaggctaaa gaagtatgc gaacacgca atgactttg gccaacgaa aatcgtgat aataacacc gtggaaggt ttagtgata accgaaacc ggtcttccg ggcgttttc ttcagtatg ttagacact gaaaccgac gctgcccta atcaaagac atccacgat acgttaatc agcatgcta caaatgctc gcagctgac aacttaagc caatggttg ggcttgtgc aaggacgtg ctaaccatc gcaaccgat agcacaaac atggaagac caacaaagc gtctcagct ccggacgaa aacgacgaa cctgaggca gacgacgac caagcagag tttcacgct gaggacgac tcttccacc caccccgcg gtgcagccc cgatggcca acgcgtgtt tttgcagcc gaatgcgtc cgaaaaata atcacagcc tgcgaaagc agcgcaaac aaaacgatt catttcgat ctaatccaa gccaaagag atggggaaa agcgatttc ctcgtcttg catttatcc gaactgatc cgaatggcg ttcattgcc gcgacgagc gactcggac ccgttgcgt ctcgaaggc ttgaaaacc ctccaagat ataatcgag aagttttcc aaagttcca gaaccggag ttccccggc cacttgtta ctcgagcag ttccaagcg caagtcggt gcagcgcta agaccagct ttcgcaccc gatacttcc tcacatgtt acagcagcc gcttgcgaa gtttgctcg acttggatc ggctcgaac gtggcacgg gatttaaat gatttgcgc cgcgtgcac caactacta gtctcatcc ttgactaag ttgcaaaat aagacgaac acaacgcaa ctctacaac gagagctta gcaacttta gagaagttg gcgattctg aaggcttgg gccgaggtt tacattgtt gcaatgaaa ggaaatgaa agtgcgaat ttggacgta acaacggcc aatgccaat gatgacgag tttggcgat tttgaatac aagggagag aatttgttg actttggtg caacccgag ttggttagt ttgtcgcaa cattggctg gccgcactg aaagaccat gccttattg tcactaccg gcagaattc gccagtcag ttgccacac gacggtggc gctttttac accaatgat acaatggaa tcgtcacga ccgcactac atatcctca tggcctcca atcctacat gctgcagct ctgtggctc aactccggc ggttttgat aaacttgcc gaaatagac aacgataat acaaacaat aacacggtt gatacgtcc agcgataag ttccatctc ttgtttggc atatgcatg gaggcgttg tgcagcccc cgatcggtc gaacccctc gaaagtata ataacatgt cttaaagca ctgtttact ctcctggac tcaacatac gcccgcgag ttgattctc gtcgacaac tcgcttggg attgaattg tgcaacgtt ttgcaccgc ctcatcctc acacgtgat aaccacacg tgccaactc ttatgcatg gaagtcctc aagcaagtg atcaaagca gcgcgcgaa aaactcgac cgcgctaag aaacaatca acgaccgat tccgattta ctgggcgaa ggtggcgaa gctggcgac attttaccc ggcaaatcg ctcgttttc tcaattttg gaagtatgt ctgtgtctg ttggttcgc caggtgcca tcgctaagc ccctcgccc aactcgaca atcgtcaat tcgctgcga atcatgcaa acgccggac gaatccggc cgcttgatc gcctcgtct ttggcctgc atggaaaat ttacacagg ttgtgttcg ccgcaaggc gccctatcc atactccca acaatcctg tatttgacc accggggtg atcaaggaa atggcgacg aaaaatatc aacgacgtg acggtgcta gcgaataac gcctcggtc caagcggcc ctccactgt ctcaaaatc ctcacaacg gatagatat tgcaacgac ccccgaagc tgtgatacc tggcagaag ctgctccag agcgccctc gccaaaatc atcgatctt gcgaaaacc ggcagcgat gagaataaa ctcgatgaa gtcacaatg atgctagcc atcgcggtt ttcgtcctc catgcgcct tcgaaagtt gtaacagcc cccaatttg caatacccc tgcattaac cacttccga caatgcctc caaagctcg aattcgacg gtaaggttg aaatgtgtt caaactttg aaatctttg tttgctcat ccggatcgg aaagtcgca actccctat attcacaca ctcgctccg cggctcgtc gaatttttg tattccgat gctgcgagg aatattttt tcagacggg gagcttttt gttacgttg gaaactatc gtaacggtt gaatcactg atcgagttg gcggaacca caaaatcga attcagatg ctcaccctt ctggttccg atcttgata agttatttg ttggaaggt caggcgctt aggtcagcg aataaatac tcgttgaat ttgcacgaa atcagcttg caaagactg atgaaaatc ggaccaaaa tatccccag gagtttaaa aaattaatg agtcagact agtgagctt agaacgaaa ttagaaaat gcgattaga tcaaaacaa caaaaccac atcaaaatc aagaacgga gtcaatgcg aataaaccc ataacgagc catcaccct tcaattaaa ttaaaaacc gactttagt aatttttcc taa
- >TC004631 RA
caggttggc agctctgcc agctgacat tggtttttg tgtcaactg tggtttttt gtgtgatta tcttgtttg tgctttttt tccgaaaaa taggcttat ttattatga caccagaaa aaaaggctt aattaaaat cagcaattg agactgact ttgaatatt ttttctatc atggaacta tcgcatagt ttaacgctt aatgaggaa gctttggct caaatcccg ccagcgaag cgacccgtt tttatattc gaatggctt cgattttta gacaaagtt ttagtcgcc gcacagaag aacgacatc aaaggatgt cagaagaaa ttagtagaa caactaatg aaccacatt caagaatct ccgggccct ccaacgcgg aaattaata gcccgctct ctcgcgacc ctcttttca gtcggagac actttcctg ctcttcgac acggtgaac aaatgcaac gatattttg aaaaataaa gacgactct cccagtttc ctcccgacg cgattagct gcgatttgc tgcgtgggg acaatgtac gaaaagctg ggccgaatg atgggccgt tcctacgag gagactgtg caaatttta actcgctcc ctccgcagc gccgagtcc caaactcga atcgaaatc atgttgacg ctcgaaaaa gtgtgtgcc gggatggga aacgctatt tcaaatgtt cataaagat atttacaaa gcggcaaga cattgcctg attgatcgg gtcatggcg gtgagatgt gccgccacc agatgtctt ctcgaaatg ttgaatcat gcccccttt ctttacacc agcgaattg gaaagtctg gcgactttg tgctttcgg gcctttgat ggctcgaat tacgaagtc agatgcgcg gtcgctaaa ctgttagga gcgcttatc gccatgact cagaaccag aaacccgaa aagacaccg cagctcaag gggctgaag ctggtgtcg ttggacgag gctttgggc atcctcatg gcgggtttt ttgcgtggg ggtgtgggt tttttgaaa ggcacagga gagataatt aagggtagt tcgagtgtt aaccgcgaa gtccgagtc ggagtaaca cacgcgtat gtgattttt gtccaaatt ttgggcagt gtgtggctt gagcgtaac ataaaagct ttcctttcg cacatcttg tatttggtc gcgaatccg aaagcggcg tcttcgcac gtcgatgct gtctattcc cggaaatgt ataaatttt attttgagg agtgtgttg gggaaaatg ttgggagag aaagcacaa acttcggcg tgtaaagaa atcgcacag attattgtt aaagaaatg aactcgatc gattttaat cccgagaat gcgaaagac ttcaatcag gagacgctt ttcagccag catttacta gtgtgtgcc ttgcaagaa atcgggtgt ttggttttg agtttaggg acgacggca cacgacttg atcaccgac caaactttg aatttgatc gacgctact gctagtgtt ttgatccat ccgtgccaa gcagcgcgt cttgcagct gcttggtgc ttgcggtgt atttgtgtg gctgttcct agccaaatc acccctttg atagaccgc tgtgttaac ggaatcgag gaatacagg acttcgccg gaagctata gccggctat agtggagct ttagctgcg gttctgggc ggtgttaac ttatccccg cttggagtg ccacacact aaaggcaaa atcattttt aacacagcg gaagagttg ctaagaagt gccagtcaa aatagccgg ctgtcgcta aaccggact caagccggt tggctactt atcggtgct attatgaca ctgggggtt ccagtagtt agggggttg ctccctcgc atgttgctc ctttggagg aattctttc cccaggtct actaaagaa ctagattcg gaaaaagcc cgaggtgat gctttcacc tggcaagtg actctcgaa ggccgcgct ggggccctg tctgctatg cacagcttc ctccaacac tgtcacgag ttagtcact gatgatatt aacagaagg ttgctggcg ccaatcgaa tctgctcta gcaatgcta tcaaacatt tcgagtatt ttgaaaagt tacggacaa cagttgaag gctccggcg gccatggtc cgcttacgc ctctacgaa accctgctg ttgcttccg ccgcaatct ttcgaaagc tcgtacact catcttttg cgaatgttg gtcgcggaa tttactttg accgaaaat ccggcaaac accacaacg tcccagatg cggactgtg tgccatgct gacgattcc gtaatttta ggcacttgg ttacaagaa actgatcat agaacaatc gaggatcaa ttacagccg aacagtgcg gccggttct ggagccctt gaacacgac ccgtgctgt ctctaccgc caaatttca acaagcgaa gtaattccg ggtccactc cctctggga gttgcagtg atcgacatg tctgttctc ctcttcggc cagattttc ccacgtgtg gccaataag caccgcttg caaatggtc gaccatttc gccgaatgc atcaaacac gcgaagagt tcgcgccag gaagcggtg caaatgaac gtatttacg gcccttttg agcggactg aaaggccta accgaggcc aaaatgagc ataggccag gaggacgtc aaaaaagcc gttacgggc ctaattatc ggtgcttta acttcaccg aacccaatt ctgcggtgc gcggcggga gaagccgtc ggacgtatg gctcaagta gtatccgac ccaaaattc accgcagaa ttggctcag acgagcttc gaccgactt aagtcagca cgtgatgta gcaagccga actgggcat tcgttagcc ctcggctgc ttgcaccgc tacgttgga ggtatgggc tccagccaa cacttgaat acgagtgtt tcaattttg ctggcgctt gcgcaagac cagacctcg ccagtggtt caagtgtgg tcgctacat gcacttgca ctaatcgcg gattcgggg ggtcccatg tttcggggt tacgtcgaa ccgagtctt tccctagct ttgaaactt ctactaaat gtaccacag tcgtatatt gacgttcat cagtgtatt gggaaagtt ctctcggcc ttaattacg acaatcggg cccgaattg caaggcaat acgtcgagt atttgtatg gcaaggagt tcttttttg tgtgcttgt gccatcatg caagaccac caagacccg ttggtccaa gcggaagcc acaggatgt ctccaacaa ctgcattta ttcgcaccg agacatgtt aatttatcg tcactagtc ccgacactt tgtcgcact ttatcgagt aatcatctt ttgttacgt aaagctgcc atttcctgt ctaagacaa ttggcacaa agggaggct aaagaagta tgcgaacac gcaatgact ttggccaac gaaaatcgt gataataac accgtggaa ggtttagtg ataaccgaa accggtctt ccgggcgtt ttcttcagt atgttagac actgaaacc gacgctgcc ctaatcaaa gacatccac gatacgtta atcagcatg ctacaaatg ctcgcagct gacaactta agccaatgg ttgggcttg tgcaaggac gtgctaacc atcgcaacc gatagcaca aacatggaa gaccaacaa agcgtctca gctccggac gaaaacgac gaacctgag gcagacgac gaccaagca gagtttcac gctgaggac gactcttcc acccacccc gcggtgcag ccccgatgg ccaacgcgt gtttttgca gccgaatgc gtccgaaaa ataatcaca gcctgcgaa agcagcgca aacaaaacg attcatttc gatctaatc caagccaaa gagatgggg aaaagcgat ttcctcgtc ttgcattta tccgaactg atccgaatg gcgttcatt gccgcgacg agcgactcg gacccgttg cgtctcgaa ggcttgaaa accctccaa gatataatc gagaagttt tccaaagtt ccagaaccg gagttcccc ggccacttg ttactcgag cagttccaa gcgcaagtc ggtgcagcg ctaagacca gctttcgca cccgatact tcctcacat gttacagca gccgcttgc gaagtttgc tcgacttgg atcggctcg aacgtggca cgggattta aatgatttg cgccgcgtg caccaacta ctagtctca tccttgact aagttgcaa aataagacg aacacaacg caactctac aacgagagc ttagcaact ttagagaag ttggcgatt ctgaaggct tgggccgag gtttacatt gttgcaatg aaaggaaat gaaagtgcg aatttggac gtaacaacg gccaatgcc aatgatgac gagtttggc gattttgaa tacaaggga gagaatttg ttgactttg gtgcaaccc gagttggtt agtttgtcg caacattgg ctggccgca ctgaaagac catgcctta ttgtcacta ccggcagaa ttcgccagt cagttgcca cacgacggt ggcgctttt tacaccaat gatacaatg gaatcgtca cgaccgcac tacatatcc tcatggcct ccaatccta catgctgca gctctgtgg ctcaactcc ggcggtttt gataaactt gccgaaata gacaacgat aatacaaac aataacacg gttgatacg tccagcgat aagttccat ctcttgttt ggcatatgc atggaggcg ttgtgcagc ccccgatcg gtcgaaccc ctcgaaagt ataataaca tgtcttaaa gcactgttt actctcctg gactcaaca tacgcccgc gagttgatt ctcgtcgac aactcgctt gggattgaa ttgtgcaac gttttgcac cgcctcatc ctcacacgt gataaccac acgtgccaa ctcttatgc atggaagtc ctcaagcaa gtgatcaaa gcagcgcgc gaaaaactc gaccgcgct aagaaacaa tcaacgacc gattccgat ttactgggc gaaggtggc gaagctggc gacatttta cccggcaaa tcgctcgtt ttctcaatt ttggaagta tgtctgtgt ctgttggtt cgccaggtg ccatcgcta agcccctcg cccaactcg acaatcgtc aattcgctg cgaatcatg caaacgccg gacgaatcc ggccgcttg atcgcctcg tctttggcc tgcatggaa aatttacac aggttgtgt tcgccgcaa ggcgcccta tccatactc ccaacaatc ctgtatttg accaccggg gtgatcaag gaaatggcg acgaaaaat atcaacgac gtgacggtg ctagcgaat aacgcctcg gtccaagcg gccctccac tgtctcaaa atcctcaca acggataga tattgcaac gacccccga agctgtgat acctggcag aagctgctc cagagcgcc ctcgccaaa atcatcgat cttgcgaaa accggcagc gatgagaat aaactcgat gaagtcaca atgatgcta gccatcgcg gttttcgtc ctccatgcg ccttcgaaa gttgtaaca gcccccaat ttgcaatac ccctgcatt aaccacttc cgacaatgc ctccaaagc tcgaattcg acggtaagg ttgaaatgt gttcaaact ttgaaatct ttgtttgct catccggat cggaaagtc gcaactccc tatattcac acactcgct ccgcggctc gtcgaattt ttgtattcc gatgctgcg aggaatatt ttttcagac ggggagctt tttgttacg ttggaaact atcgtaacg gttgaatca ctgatcgag ttggcggaa ccacaaaat cgaattcag atgctcacc cttctggtt ccgatcttg ataagttat ttgttggaa ggtcaggcg cttaggtca gcgaataaa tactcgttg aatttgcac gaaatcagc ttgcaaaga ctgatgaaa atcggacca aaatatccc caggagttt aaaaaatta atgagtcag actagtgag cttagaacg aaattagaa aatgcgatt agatcaaaa caacaaaac cacatcaaa atcaagaac ggagtcaat gcgaataaa cccataacg agccatcac ccttcaatt aaattaaaa accgacttt agtaatttt tcctaagtt acgtgatgg gttctagtg attacaat
- >TC004631 RB
caggttggc agctctgcc agctgacat tggtttttg tgtcaactg tggtttttt gtgtgatta tcttgtttg tgctttttt tccgaaaaa taggcttat ttattatga caccagaaa aaaaggctt aattaaaat cagcaattg agactgact ttgaatatt ttttctatc atggaacta tcgcatagt ttaacgctt aatgaggaa gctttggct caaatcccg ccagcgaag cgacccgtt tttatattc gaatggctt cgattttta gacaaagtt ttagtcgcc gcacagaag aacgacatc aaaggatgt cagaagaaa ttagtagaa caactaatg aaccacatt caagaatct ccgggccct ccaacgcgg aaattaata gcccgctct ctcgcgacc ctcttttca gtcggagac actttcctg ctcttcgac acggtgaac aaatgcaac gatattttg aaaaataaa gacgactct cccagtttc ctcccgacg cgattagct gcgatttgc tgcgtgggg acaatgtac gaaaagctg ggccgaatg atgggccgt tcctacgag gagactgtg caaatttta actcgctcc ctccgcagc gccgagtcc caaactcga atcgaaatc atgttgacg ctcgaaaaa gtgtgtgcc gggatggga aacgctatt tcaaatgtt cataaagat atttacaaa gcggcaaga cattgcctg attgatcgg gtcatggcg gtgagatgt gccgccacc agatgtctt ctcgaaatg ttgaatcat gcccccttt ctttacacc agcgaattg gaaagtctg gcgactttg tgctttcgg gcctttgat ggctcgaat tacgaagtc agatgcgcg gtcgctaaa ctgttagga gcgcttatc gccatgact cagaaccag aaacccgaa aagacaccg cagctcaag gggctgaag ctggtgtcg ttggacgag gctttgggc atcctcatg gcgggtttt ttgcgtggg ggtgtgggt tttttgaaa ggcacagga gagataatt aagggtagt tcgagtgtt aaccgcgaa gtccgagtc ggagtaaca cacgcgtat gtgattttt gtccaaatt ttgggcagt gtgtggctt gagcgtaac ataaaagct ttcctttcg cacatcttg tatttggtc gcgaatccg aaagcggcg tcttcgcac gtcgatgct gtctattcc cggaaatgt ataaatttt attttgagg agtgtgttg gggaaaatg ttgggagag aaagcacaa acttcggcg tgtaaagaa atcgcacag attattgtt aaagaaatg aactcgatc gattttaat cccgagaat gcgaaagac ttcaatcag gagacgctt ttcagccag catttacta gtgtgtgcc ttgcaagaa atcgggtgt ttggttttg agtttaggg acgacggca cacgacttg atcaccgac caaactttg aatttgatc gacgctact gctagtgtt ttgatccat ccgtgccaa gcagcgcgt cttgcagct gcttggtgc ttgcggtgt atttgtgtg gctgttcct agccaaatc acccctttg atagaccgc tgtgttaac ggaatcgag gaatacagg acttcgccg gaagctata gccggctat agtggagct ttagctgcg gttctgggc ggtgttaac ttatccccg cttggagtg ccacacact aaaggcaaa atcattttt aacacagcg gaagagttg ctaagaagt gccagtcaa aatagccgg ctgtcgcta aaccggact caagccggt tggctactt atcggtgct attatgaca ctgggggtt ccagtagtt agggggttg ctccctcgc atgttgctc ctttggagg aattctttc cccaggtct actaaagaa ctagattcg gaaaaagcc cgaggtgat gctttcacc tggcaagtg actctcgaa ggccgcgct ggggccctg tctgctatg cacagcttc ctccaacac tgtcacgag ttagtcact gatgatatt aacagaagg ttgctggcg ccaatcgaa tctgctcta gcaatgcta tcaaacatt tcgagtatt ttgaaaagt tacggacaa cagttgaag gctccggcg gccatggtc cgcttacgc ctctacgaa accctgctg ttgcttccg ccgcaatct ttcgaaagc tcgtacact catcttttg cgaatgttg gtcgcggaa tttactttg accgaaaat ccggcaaac accacaacg tcccagatg cggactgtg tgccatgct gacgattcc gtaatttta ggcacttgg ttacaagaa actgatcat agaacaatc gaggatcaa atggagcct aaccgaagg gcagatggt gaacattta cagccgaac agtgcggcc ggttctgga gcccttgaa cacgacccg tgctgtctc taccgccaa atttcaaca agcgaagta attccgggt ccactccct ctgggagtt gcagtgatc gacatgtct gttctcctc ttcggccag attttccca cgtgtggcc aataagcac cgcttgcaa atggtcgac catttcgcc gaatgcatc aaacacgcg aagagttcg cgccaggaa gcggtgcaa atgaacgta tttacggcc cttttgagc ggactgaaa ggcctaacc gaggccaaa atgagcata ggccaggag gacgtcaaa aaagccgtt acgggccta attatcggt gctttaact tcaccgaac ccaattctg cggtgcgcg gcgggagaa gccgtcgga cgtatggct caagtagta tccgaccca aaattcacc gcagaattg gctcagacg agcttcgac cgacttaag tcagcacgt gatgtagca agccgaact gggcattcg ttagccctc ggctgcttg caccgctac gttggaggt atgggctcc agccaacac ttgaatacg agtgtttca attttgctg gcgcttgcg caagaccag acctcgcca gtggttcaa gtgtggtcg ctacatgca cttgcacta atcgcggat tcggggggt cccatgttt cggggttac gtcgaaccg agtctttcc ctagctttg aaacttcta ctaaatgta ccacagtcg tatattgac gttcatcag tgtattggg aaagttctc tcggcctta attacgaca atcgggccc gaattgcaa ggcaatacg tcgagtatt tgtatggca aggagttct tttttgtgt gcttgtgcc atcatgcaa gaccaccaa gacccgttg gtccaagcg gaagccaca ggatgtctc caacaactg catttattc gcaccgaga catgttaat ttatcgtca ctagtcccg acactttgt cgcacttta tcgagtaat catcttttg ttacgtaaa gctgccatt tcctgtcta agacaattg gcacaaagg gaggctaaa gaagtatgc gaacacgca atgactttg gccaacgaa aatcgtgat aataacacc gtggaaggt ttagtgata accgaaacc ggtcttccg ggcgttttc ttcagtatg ttagacact gaaaccgac gctgcccta atcaaagac atccacgat acgttaatc agcatgcta caaatgctc gcagctgac aacttaagc caatggttg ggcttgtgc aaggacgtg ctaaccatc gcaaccgat agcacaaac atggaagac caacaaagc gtctcagct ccggacgaa aacgacgaa cctgaggca gacgacgac caagcagag tttcacgct gaggacgac tcttccacc caccccgcg gtgcagccc cgatggcca acgcgtgtt tttgcagcc gaatgcgtc cgaaaaata atcacagcc tgcgaaagc agcgcaaac aaaacgatt catttcgat ctaatccaa gccaaagag atggggaaa agcgatttc ctcgtcttg catttatcc gaactgatc cgaatggcg ttcattgcc gcgacgagc gactcggac ccgttgcgt ctcgaaggc ttgaaaacc ctccaagat ataatcgag aagttttcc aaagttcca gaaccggag ttccccggc cacttgtta ctcgagcag ttccaagcg caagtcggt gcagcgcta agaccagct ttcgcaccc gatacttcc tcacatgtt acagcagcc gcttgcgaa gtttgctcg acttggatc ggctcgaac gtggcacgg gatttaaat gatttgcgc cgcgtgcac caactacta gtctcatcc ttgactaag ttgcaaaat aagacgaac acaacgcaa ctctacaac gagagctta gcaacttta gagaagttg gcgattctg aaggcttgg gccgaggtt tacattgtt gcaatgaaa ggaaatgaa agtgcgaat ttggacgta acaacggcc aatgccaat gatgacgag tttggcgat tttgaatac aagggagag aatttgttg actttggtg caacccgag ttggttagt ttgtcgcaa cattggctg gccgcactg aaagaccat gccttattg tcactaccg gcagaattc gccagtcag ttgccacac gacggtggc gctttttac accaatgat acaatggaa tcgtcacga ccgcactac atatcctca tggcctcca atcctacat gctgcagct ctgtggctc aactccggc ggttttgat aaacttgcc gaaatagac aacgataat acaaacaat aacacggtt gatacgtcc agcgataag ttccatctc ttgtttggc atatgcatg gaggcgttg tgcagcccc cgatcggtc gaacccctc gaaagtata ataacatgt cttaaagca ctgtttact ctcctggac tcaacatac gcccgcgag ttgattctc gtcgacaac tcgcttggg attgaattg tgcaacgtt ttgcaccgc ctcatcctc acacgtgat aaccacacg tgccaactc ttatgcatg gaagtcctc aagcaagtg atcaaagca gcgcgcgaa aaactcgac cgcgctaag aaacaatca acgaccgat tccgattta ctgggcgaa ggtggcgaa gctggcgac attttaccc ggcaaatcg ctcgttttc tcaattttg gaagtatgt ctgtgtctg ttggttcgc caggtgcca tcgctaagc ccctcgccc aactcgaca atcgtcaat tcgctgcga atcatgcaa acgccggac gaatccggc cgcttgatc gcctcgtct ttggcctgc atggaaaat ttacacagg ttgtgttcg ccgcaaggc gccctatcc atactccca acaatcctg tatttgacc accggggtg atcaaggaa atggcgacg aaaaatatc aacgacgtg acggtgcta gcgaataac gcctcggtc caagcggcc ctccactgt ctcaaaatc ctcacaacg gatagatat tgcaacgac ccccgaagc tgtgatacc tggcagaag ctgctccag agcgccctc gccaaaatc atcgatctt gcgaaaacc ggcagcgat gagaataaa ctcgatgaa gtcacaatg atgctagcc atcgcggtt ttcgtcctc catgcgcct tcgaaagtt gtaacagcc cccaatttg caatacccc tgcattaac cacttccga caatgcctc caaagctcg aattcgacg gtaaggttg aaatgtgtt caaactttg aaatctttg tttgctcat ccggatcgg aaagtcgca actccctat attcacaca ctcgctccg cggctcgtc gaatttttg tattccgat gctgcgagg aatattttt tcagacggg gagcttttt gttacgttg gaaactatc gtaacggtt gaatcactg atcgagttg gcggaacca caaaatcga attcagatg ctcaccctt ctggttccg atcttgata agttatttg ttggaaggt caggcgctt aggtcagcg aataaatac tcgttgaat ttgcacgaa atcagcttg caaagactg atgaaaatc ggaccaaaa tatccccag gagtttaaa aaattaatg agtcagact agtgagctt agaacgaaa ttagaaaat gcgattaga tcaaaacaa caaaaccac atcaaaatc aagaacgga gtcaatgcg aataaaccc ataacgagc catcaccct tcaattaaa ttaaaaacc gactttagt aatttttcc taagttacg tgatgggtt ctagtgatt acaat
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