| General information | |||
| TC004609 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 6655511..6659555 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004609 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00734 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
egg chamber potentially fused - Number of animals showing the phenotype: 4
vitellarium shape irregular - Number of animals showing the phenotype: 4
vitellarium size increased - Number of animals showing the phenotype: 4
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (16 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 6 (22 dpi)
- >TC004609 RA
MNDHFGLKW DPKNLEIRT MSVEKTLEP LVLQVTTLV NTKGPSKKK KGKSKRANA LVSTVEKAT ENFIEKGEQ IAYENPDIT QEMLSAVEE VRKTGNAMS IAAREFSED PCSSLKRGN MVRAARNLL SAVTRLLIL ADMVDVHLL LKSLHVVEN DIEKLKNAS SQGELLDNI KAFGQNANE LMNQAAKRQ QELKDPQLR DDLAAARAV LKKHSTMLL TASKVYVRH PELAAAKAN RDYVLKQVC EAVNTINDV AQGRTPQPA TGPYDGPGE LAAALDDFD DHMVMEPLA YNEVHTRPS LEERLESII SGAALMADS SCTRDERRE RIVAECNAV RQALQDLLS EYMSNIGNK EKSESLNRA IDNMGRKTR DLRRQLRKA VVDHVSDSF LETNVPLLV LIKAAQNGN EKEVEEFAV MFTEHSNKL VEVANLVCS MSNNEDGVK MVRYAAAQI DNLCPEVIN AARILAARP RSKVAQENM DAFKQSWEN QVRILTEAV DDITTIDDF LAVSENHIL EDVNKCVLA LQEGDADTL DRTAGGIRG RSNRVCNVV TAEMDNYEP CIYTKRVLE AVKVLNDQV MPKFSQRVQ VAVQALSSV PTKEVDEND FIDASRLVY DGVREIRRA VLMNRADED LDPEDVELD ENYTLETRS KSSAHTGEH GVDEYPDIS GITTAREAM GKMPEEDKQ KILQQVEYF RSEKLKFDR EVAKWDDTG NDIIVLAKH MCMIMMEMT DFTRGRGPL KTTMDVINA AKKISEAGT KLDKLTRQI AEQCPESST KKDLLAYLQ RIALYCHQM NITSKVKAD VQNISGELI VSGLDSATS LIQAAKNLM NAVVLTVKA SYVASTKYP RQGTISSPI VVWKMKAPE KKPLVRPEK PEEVRAKVR KGSQKKVQN PIHALSEFQ SPTESI
- >TC004609 RA
atgaatgat cattttggg ttaaagtgg gacccgaag aatttagaa ataaggaca atgtcagtt gaaaagaca ttggagcct ttggtttta caagtcacg actcttgta aataccaaa ggccccagc aaaaagaaa aagggcaag tcgaagaga gctaacgct cttgtcagc accgtggag aaggccacg gagaatttt attgaaaaa ggcgaacaa atcgcttac gaaaacccc gacataacc caagaaatg ctctctgct gttgaggaa gttcgcaaa accggtaac gcgatgagt atcgccgct cgtgaattc tctgaagac ccttgttcc tccctgaaa cggggcaat atggtgcgt gctgcacga aatcttctt tccgctgta acccgtctt ttgattttg gctgacatg gtcgatgtt catctcttg ctcaaatcg cttcacgtc gtcgagaac gatatcgaa aaactgaaa aatgcgtca agtcaaggc gaactttta gataatatc aaggccttt ggccagaat gccaacgaa ctgatgaat caggcagct aagcgccaa caggagttg aaggatcct cagctgaga gatgattta gctgctgct cgtgctgta cttaagaaa cattccact atgttactg acagcttca aaagtctac gtccgtcat ccggagttg gcagctgcg aaagctaac cgtgattat gtgctgaag caagtctgt gaagctgtt aatacgatc aatgatgtc gctcaagga cggacaccg cagccggct acaggccca tacgatggt cctggagaa ttagctgca gctctggat gactttgac gatcatatg gttatggag ccgttggct tacaacgag gttcacacg agaccgtct ctggaggaa cgtttggag agtatcatt agtggtgca gctcttatg gccgactcc agctgcacc cgagacgaa agaagggaa cgtatagtt gccgaatgt aatgctgtc cgtcaagca cttcaggac ttgctttct gagtacatg tcaaacatc ggcaataag gaaaagagc gaaagtttg aatcgagcc atcgacaat atgggccgc aaaactcgc gatctgaga cggcagttg cgcaaagct gtcgtcgac cacgtttct gacagtttt ctcgaaaca aacgttcct ctcttggtt ttgatcaaa gccgctcaa aatggtaac gagaaggaa gtcgaggag tttgccgtt atgttcact gaacactcg aataagctc gtcgaagtt gccaacttg gtgtgcagt atgtcaaat aacgaggac ggtgtcaaa atggttcgc tacgcagcg gcacagatt gacaacctc tgtccggaa gtaatcaac gctgctagg attctagca gcgcgacca cgtagcaaa gttgctcaa gaaaacatg gacgctttt aaacaatca tgggagaat caagtccgg attttgact gaagcagtt gacgatatt acaacaatt gatgacttt ttggctgta tcggaaaat catattcta gaagatgta aataagtgt gtgttggcg ttgcaagaa ggcgatgcc gataccttg gacagaact gcaggaggt attagaggg cgctcaaat cgtgtctgt aatgttgtc actgctgaa atggataat tatgaacct tgtatttac actaagcgc gttttggaa gccgttaaa gtattaaac gatcaagtg atgcccaaa tttagtcaa agggtccaa gttgccgtt caagctttg tctagcgtt cccaccaaa gaggttgac gagaatgat tttattgac gcatcacga ttggtgtac gatggagtg cgtgaaatt agaagagcc gtcctgatg aatagagct gatgaagat ttggacccc gaagatgta gaattggat gaaaattat acattagaa acccgtagc aaatcgagt gctcacact ggcgaacat ggtgtggat gaatatcct gacatcagt ggtattacc acggctaga gaagctatg gggaaaatg cctgaggag gacaaacaa aaaatcctc caacaggta gaatatttc cgtagcgaa aaactcaaa tttgacaga gaagtcgcc aagtgggac gataccggt aatgatatt atcgttttg gctaaacat atgtgcatg attatgatg gagatgacg gattttaca cgtggacgt ggacctctg aaaactaca atggacgtc ataaacgcg gcgaaaaaa atttcagaa gccggaaca aaattggac aaattgaca cgtcaaatt gccgagcaa tgtccggag agttctacc aagaaagat cttttggcc tatttgcaa cgtattgct ttatattgc caccaaatg aacattacg tcgaaagtt aaggctgat gttcagaat ataagtgga gaattgatc gtttcaggg cttgacagc gccacttcg ctcattcaa gctgcaaag aatctcatg aatgctgta gtattgact gtaaaggcg tcatatgta gcatccacc aaatatccg cggcaaggg accatttcg tcgccaatt gtcgtctgg aagatgaaa gcaccggaa aagaaaccg ctagtaaga cctgaaaag cctgaagaa gttcgggct aaagtccgt aaagggtcg caaaagaag gtgcagaat cctatacat gcgctttct gaattccaa agcccaaca gaatcaatt taa
- >TC004609 RA
gttcttgtg ccaacttta cactgacgc tagttagaa ataatttat acacaaaag tcctttttt tccgttgtt tggtttatg attaacaaa aagtcttat tcaattatt ttggatgta cagtaaggt tgtcatttg ataagataa agttgagta ttgataaga aaagtgaca ttacaagta gtgggagtg gttcaattt gatgttttg ttagtatgg ctactctgg agtgaggta gaggtatgt aattttttc agtcatgaa ctaatgact gttcagtgg tgattcatc gtcacagtt tattgcagt tgagtgtct ggaattttt acttaaagt aaaatctct gtgtgtgat aggagggaa gaatcttgg gaaaaatag atctgccat aatgaatga tcattttgg gttaaagtg ggacccgaa gaatttaga aataaggac aatgtcagt tgaaaagac attggagcc tttggtttt acaagtcac gactcttgt aaataccaa aggccccag caaaaagaa aaagggcaa gtcgaagag agctaacgc tcttgtcag caccgtgga gaaggccac ggagaattt tattgaaaa aggcgaaca aatcgctta cgaaaaccc cgacataac ccaagaaat gctctctgc tgttgagga agttcgcaa aaccggtaa cgcgatgag tatcgccgc tcgtgaatt ctctgaaga cccttgttc ctccctgaa acggggcaa tatggtgcg tgctgcacg aaatcttct ttccgctgt aacccgtct tttgatttt ggctgacat ggtcgatgt tcatctctt gctcaaatc gcttcacgt cgtcgagaa cgatatcga aaaactgaa aaatgcgtc aagtcaagg cgaactttt agataatat caaggcctt tggccagaa tgccaacga actgatgaa tcaggcagc taagcgcca acaggagtt gaaggatcc tcagctgag agatgattt agctgctgc tcgtgctgt acttaagaa acattccac tatgttact gacagcttc aaaagtcta cgtccgtca tccggagtt ggcagctgc gaaagctaa ccgtgatta tgtgctgaa gcaagtctg tgaagctgt taatacgat caatgatgt cgctcaagg acggacacc gcagccggc tacaggccc atacgatgg tcctggaga attagctgc agctctgga tgactttga cgatcatat ggttatgga gccgttggc ttacaacga ggttcacac gagaccgtc tctggagga acgtttgga gagtatcat tagtggtgc agctcttat ggccgactc cagctgcac ccgagacga aagaaggga acgtatagt tgccgaatg taatgctgt ccgtcaagc acttcagga cttgctttc tgagtacat gtcaaacat cggcaataa ggaaaagag cgaaagttt gaatcgagc catcgacaa tatgggccg caaaactcg cgatctgag acggcagtt gcgcaaagc tgtcgtcga ccacgtttc tgacagttt tctcgaaac aaacgttcc tctcttggt tttgatcaa agccgctca aaatggtaa cgagaagga agtcgagga gtttgccgt tatgttcac tgaacactc gaataagct cgtcgaagt tgccaactt ggtgtgcag tatgtcaaa taacgagga cggtgtcaa aatggttcg ctacgcagc ggcacagat tgacaacct ctgtccgga agtaatcaa cgctgctag gattctagc agcgcgacc acgtagcaa agttgctca agaaaacat ggacgcttt taaacaatc atgggagaa tcaagtccg gattttgac tgaagcagt tgacgatat tacaacaat tgatgactt tttggctgt atcggaaaa tcatattct agaagatgt aaataagtg tgtgttggc gttgcaaga aggcgatgc cgatacctt ggacagaac tgcaggagg tattagagg gcgctcaaa tcgtgtctg taatgttgt cactgctga aatggataa ttatgaacc ttgtattta cactaagcg cgttttgga agccgttaa agtattaaa cgatcaagt gatgcccaa atttagtca aagggtcca agttgccgt tcaagcttt gtctagcgt tcccaccaa agaggttga cgagaatga ttttattga cgcatcacg attggtgta cgatggagt gcgtgaaat tagaagagc cgtcctgat gaatagagc tgatgaaga tttggaccc cgaagatgt agaattgga tgaaaatta tacattaga aacccgtag caaatcgag tgctcacac tggcgaaca tggtgtgga tgaatatcc tgacatcag tggtattac cacggctag agaagctat ggggaaaat gcctgagga ggacaaaca aaaaatcct ccaacaggt agaatattt ccgtagcga aaaactcaa atttgacag agaagtcgc caagtggga cgataccgg taatgatat tatcgtttt ggctaaaca tatgtgcat gattatgat ggagatgac ggattttac acgtggacg tggacctct gaaaactac aatggacgt cataaacgc ggcgaaaaa aatttcaga agccggaac aaaattgga caaattgac acgtcaaat tgccgagca atgtccgga gagttctac caagaaaga tcttttggc ctatttgca acgtattgc tttatattg ccaccaaat gaacattac gtcgaaagt taaggctga tgttcagaa tataagtgg agaattgat cgtttcagg gcttgacag cgccacttc gctcattca agctgcaaa gaatctcat gaatgctgt agtattgac tgtaaaggc gtcatatgt agcatccac caaatatcc gcggcaagg gaccatttc gtcgccaat tgtcgtctg gaagatgaa agcaccgga aaagaaacc gctagtaag acctgaaaa gcctgaaga agttcgggc taaagtccg taaagggtc gcaaaagaa ggtgcagaa tcctataca tgcgctttc tgaattcca aagcccaac agaatcaat ttaagtatt ttttgtaaa atttataca tttttatga atttctttt tccaatttt tgccgtaaa agatcattt ttttatttt ttgttattt attaatgtt acgtcgttg ccaacaaga atttatgta tttatgtgg gtaggagca ctatttgca ctgtgggaa atataagca atcatgaaa agttttaca tgtttgtaa ccattctat ttttgttat ttttctaat gataaatgt tctgaaaat taataaaga aagtgtggc ttccaatat ttggatatg cttaagtta attctaagt ttactttgc aagaactat tacaacttg cgtgcttac ataagtggt aataaacat catttttac at
