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Gene information
General information
TC004609 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6655511..6659555
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004609
    iBeetle screen iB_00734

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    egg chamber complete not present - Number of animals showing the phenotype: 4

    egg chamber potentially fused - Number of animals showing the phenotype: 4
    vitellarium shape irregular - Number of animals showing the phenotype: 4
    vitellarium size increased - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 6 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004609 RA
      MNDHFGLKW DPKNLEIRT MSVEKTLEP LVLQVTTLV 
      NTKGPSKKK KGKSKRANA LVSTVEKAT ENFIEKGEQ 
      IAYENPDIT QEMLSAVEE VRKTGNAMS IAAREFSED 
      PCSSLKRGN MVRAARNLL SAVTRLLIL ADMVDVHLL 
      LKSLHVVEN DIEKLKNAS SQGELLDNI KAFGQNANE 
      LMNQAAKRQ QELKDPQLR DDLAAARAV LKKHSTMLL 
      TASKVYVRH PELAAAKAN RDYVLKQVC EAVNTINDV 
      AQGRTPQPA TGPYDGPGE LAAALDDFD DHMVMEPLA 
      YNEVHTRPS LEERLESII SGAALMADS SCTRDERRE 
      RIVAECNAV RQALQDLLS EYMSNIGNK EKSESLNRA 
      IDNMGRKTR DLRRQLRKA VVDHVSDSF LETNVPLLV 
      LIKAAQNGN EKEVEEFAV MFTEHSNKL VEVANLVCS 
      MSNNEDGVK MVRYAAAQI DNLCPEVIN AARILAARP 
      RSKVAQENM DAFKQSWEN QVRILTEAV DDITTIDDF 
      LAVSENHIL EDVNKCVLA LQEGDADTL DRTAGGIRG 
      RSNRVCNVV TAEMDNYEP CIYTKRVLE AVKVLNDQV 
      MPKFSQRVQ VAVQALSSV PTKEVDEND FIDASRLVY 
      DGVREIRRA VLMNRADED LDPEDVELD ENYTLETRS 
      KSSAHTGEH GVDEYPDIS GITTAREAM GKMPEEDKQ 
      KILQQVEYF RSEKLKFDR EVAKWDDTG NDIIVLAKH 
      MCMIMMEMT DFTRGRGPL KTTMDVINA AKKISEAGT 
      KLDKLTRQI AEQCPESST KKDLLAYLQ RIALYCHQM 
      NITSKVKAD VQNISGELI VSGLDSATS LIQAAKNLM 
      NAVVLTVKA SYVASTKYP RQGTISSPI VVWKMKAPE 
      KKPLVRPEK PEEVRAKVR KGSQKKVQN PIHALSEFQ 
      SPTESI
    CDS sequence
    • >TC004609 RA
      atgaatgat cattttggg ttaaagtgg gacccgaag 
      aatttagaa ataaggaca atgtcagtt gaaaagaca 
      ttggagcct ttggtttta caagtcacg actcttgta 
      aataccaaa ggccccagc aaaaagaaa aagggcaag 
      tcgaagaga gctaacgct cttgtcagc accgtggag 
      aaggccacg gagaatttt attgaaaaa ggcgaacaa 
      atcgcttac gaaaacccc gacataacc caagaaatg 
      ctctctgct gttgaggaa gttcgcaaa accggtaac 
      gcgatgagt atcgccgct cgtgaattc tctgaagac 
      ccttgttcc tccctgaaa cggggcaat atggtgcgt 
      gctgcacga aatcttctt tccgctgta acccgtctt 
      ttgattttg gctgacatg gtcgatgtt catctcttg 
      ctcaaatcg cttcacgtc gtcgagaac gatatcgaa 
      aaactgaaa aatgcgtca agtcaaggc gaactttta 
      gataatatc aaggccttt ggccagaat gccaacgaa 
      ctgatgaat caggcagct aagcgccaa caggagttg 
      aaggatcct cagctgaga gatgattta gctgctgct 
      cgtgctgta cttaagaaa cattccact atgttactg 
      acagcttca aaagtctac gtccgtcat ccggagttg 
      gcagctgcg aaagctaac cgtgattat gtgctgaag 
      caagtctgt gaagctgtt aatacgatc aatgatgtc 
      gctcaagga cggacaccg cagccggct acaggccca 
      tacgatggt cctggagaa ttagctgca gctctggat 
      gactttgac gatcatatg gttatggag ccgttggct 
      tacaacgag gttcacacg agaccgtct ctggaggaa 
      cgtttggag agtatcatt agtggtgca gctcttatg 
      gccgactcc agctgcacc cgagacgaa agaagggaa 
      cgtatagtt gccgaatgt aatgctgtc cgtcaagca 
      cttcaggac ttgctttct gagtacatg tcaaacatc 
      ggcaataag gaaaagagc gaaagtttg aatcgagcc 
      atcgacaat atgggccgc aaaactcgc gatctgaga 
      cggcagttg cgcaaagct gtcgtcgac cacgtttct 
      gacagtttt ctcgaaaca aacgttcct ctcttggtt 
      ttgatcaaa gccgctcaa aatggtaac gagaaggaa 
      gtcgaggag tttgccgtt atgttcact gaacactcg 
      aataagctc gtcgaagtt gccaacttg gtgtgcagt 
      atgtcaaat aacgaggac ggtgtcaaa atggttcgc 
      tacgcagcg gcacagatt gacaacctc tgtccggaa 
      gtaatcaac gctgctagg attctagca gcgcgacca 
      cgtagcaaa gttgctcaa gaaaacatg gacgctttt 
      aaacaatca tgggagaat caagtccgg attttgact 
      gaagcagtt gacgatatt acaacaatt gatgacttt 
      ttggctgta tcggaaaat catattcta gaagatgta 
      aataagtgt gtgttggcg ttgcaagaa ggcgatgcc 
      gataccttg gacagaact gcaggaggt attagaggg 
      cgctcaaat cgtgtctgt aatgttgtc actgctgaa 
      atggataat tatgaacct tgtatttac actaagcgc 
      gttttggaa gccgttaaa gtattaaac gatcaagtg 
      atgcccaaa tttagtcaa agggtccaa gttgccgtt 
      caagctttg tctagcgtt cccaccaaa gaggttgac 
      gagaatgat tttattgac gcatcacga ttggtgtac 
      gatggagtg cgtgaaatt agaagagcc gtcctgatg 
      aatagagct gatgaagat ttggacccc gaagatgta 
      gaattggat gaaaattat acattagaa acccgtagc 
      aaatcgagt gctcacact ggcgaacat ggtgtggat 
      gaatatcct gacatcagt ggtattacc acggctaga 
      gaagctatg gggaaaatg cctgaggag gacaaacaa 
      aaaatcctc caacaggta gaatatttc cgtagcgaa 
      aaactcaaa tttgacaga gaagtcgcc aagtgggac 
      gataccggt aatgatatt atcgttttg gctaaacat 
      atgtgcatg attatgatg gagatgacg gattttaca 
      cgtggacgt ggacctctg aaaactaca atggacgtc 
      ataaacgcg gcgaaaaaa atttcagaa gccggaaca 
      aaattggac aaattgaca cgtcaaatt gccgagcaa 
      tgtccggag agttctacc aagaaagat cttttggcc 
      tatttgcaa cgtattgct ttatattgc caccaaatg 
      aacattacg tcgaaagtt aaggctgat gttcagaat 
      ataagtgga gaattgatc gtttcaggg cttgacagc 
      gccacttcg ctcattcaa gctgcaaag aatctcatg 
      aatgctgta gtattgact gtaaaggcg tcatatgta 
      gcatccacc aaatatccg cggcaaggg accatttcg 
      tcgccaatt gtcgtctgg aagatgaaa gcaccggaa 
      aagaaaccg ctagtaaga cctgaaaag cctgaagaa 
      gttcgggct aaagtccgt aaagggtcg caaaagaag 
      gtgcagaat cctatacat gcgctttct gaattccaa 
      agcccaaca gaatcaatt taa
    mRNA sequence
    • >TC004609 RA
      gttcttgtg ccaacttta cactgacgc tagttagaa 
      ataatttat acacaaaag tcctttttt tccgttgtt 
      tggtttatg attaacaaa aagtcttat tcaattatt 
      ttggatgta cagtaaggt tgtcatttg ataagataa 
      agttgagta ttgataaga aaagtgaca ttacaagta 
      gtgggagtg gttcaattt gatgttttg ttagtatgg 
      ctactctgg agtgaggta gaggtatgt aattttttc 
      agtcatgaa ctaatgact gttcagtgg tgattcatc 
      gtcacagtt tattgcagt tgagtgtct ggaattttt 
      acttaaagt aaaatctct gtgtgtgat aggagggaa 
      gaatcttgg gaaaaatag atctgccat aatgaatga 
      tcattttgg gttaaagtg ggacccgaa gaatttaga 
      aataaggac aatgtcagt tgaaaagac attggagcc 
      tttggtttt acaagtcac gactcttgt aaataccaa 
      aggccccag caaaaagaa aaagggcaa gtcgaagag 
      agctaacgc tcttgtcag caccgtgga gaaggccac 
      ggagaattt tattgaaaa aggcgaaca aatcgctta 
      cgaaaaccc cgacataac ccaagaaat gctctctgc 
      tgttgagga agttcgcaa aaccggtaa cgcgatgag 
      tatcgccgc tcgtgaatt ctctgaaga cccttgttc 
      ctccctgaa acggggcaa tatggtgcg tgctgcacg 
      aaatcttct ttccgctgt aacccgtct tttgatttt 
      ggctgacat ggtcgatgt tcatctctt gctcaaatc 
      gcttcacgt cgtcgagaa cgatatcga aaaactgaa 
      aaatgcgtc aagtcaagg cgaactttt agataatat 
      caaggcctt tggccagaa tgccaacga actgatgaa 
      tcaggcagc taagcgcca acaggagtt gaaggatcc 
      tcagctgag agatgattt agctgctgc tcgtgctgt 
      acttaagaa acattccac tatgttact gacagcttc 
      aaaagtcta cgtccgtca tccggagtt ggcagctgc 
      gaaagctaa ccgtgatta tgtgctgaa gcaagtctg 
      tgaagctgt taatacgat caatgatgt cgctcaagg 
      acggacacc gcagccggc tacaggccc atacgatgg 
      tcctggaga attagctgc agctctgga tgactttga 
      cgatcatat ggttatgga gccgttggc ttacaacga 
      ggttcacac gagaccgtc tctggagga acgtttgga 
      gagtatcat tagtggtgc agctcttat ggccgactc 
      cagctgcac ccgagacga aagaaggga acgtatagt 
      tgccgaatg taatgctgt ccgtcaagc acttcagga 
      cttgctttc tgagtacat gtcaaacat cggcaataa 
      ggaaaagag cgaaagttt gaatcgagc catcgacaa 
      tatgggccg caaaactcg cgatctgag acggcagtt 
      gcgcaaagc tgtcgtcga ccacgtttc tgacagttt 
      tctcgaaac aaacgttcc tctcttggt tttgatcaa 
      agccgctca aaatggtaa cgagaagga agtcgagga 
      gtttgccgt tatgttcac tgaacactc gaataagct 
      cgtcgaagt tgccaactt ggtgtgcag tatgtcaaa 
      taacgagga cggtgtcaa aatggttcg ctacgcagc 
      ggcacagat tgacaacct ctgtccgga agtaatcaa 
      cgctgctag gattctagc agcgcgacc acgtagcaa 
      agttgctca agaaaacat ggacgcttt taaacaatc 
      atgggagaa tcaagtccg gattttgac tgaagcagt 
      tgacgatat tacaacaat tgatgactt tttggctgt 
      atcggaaaa tcatattct agaagatgt aaataagtg 
      tgtgttggc gttgcaaga aggcgatgc cgatacctt 
      ggacagaac tgcaggagg tattagagg gcgctcaaa 
      tcgtgtctg taatgttgt cactgctga aatggataa 
      ttatgaacc ttgtattta cactaagcg cgttttgga 
      agccgttaa agtattaaa cgatcaagt gatgcccaa 
      atttagtca aagggtcca agttgccgt tcaagcttt 
      gtctagcgt tcccaccaa agaggttga cgagaatga 
      ttttattga cgcatcacg attggtgta cgatggagt 
      gcgtgaaat tagaagagc cgtcctgat gaatagagc 
      tgatgaaga tttggaccc cgaagatgt agaattgga 
      tgaaaatta tacattaga aacccgtag caaatcgag 
      tgctcacac tggcgaaca tggtgtgga tgaatatcc 
      tgacatcag tggtattac cacggctag agaagctat 
      ggggaaaat gcctgagga ggacaaaca aaaaatcct 
      ccaacaggt agaatattt ccgtagcga aaaactcaa 
      atttgacag agaagtcgc caagtggga cgataccgg 
      taatgatat tatcgtttt ggctaaaca tatgtgcat 
      gattatgat ggagatgac ggattttac acgtggacg 
      tggacctct gaaaactac aatggacgt cataaacgc 
      ggcgaaaaa aatttcaga agccggaac aaaattgga 
      caaattgac acgtcaaat tgccgagca atgtccgga 
      gagttctac caagaaaga tcttttggc ctatttgca 
      acgtattgc tttatattg ccaccaaat gaacattac 
      gtcgaaagt taaggctga tgttcagaa tataagtgg 
      agaattgat cgtttcagg gcttgacag cgccacttc 
      gctcattca agctgcaaa gaatctcat gaatgctgt 
      agtattgac tgtaaaggc gtcatatgt agcatccac 
      caaatatcc gcggcaagg gaccatttc gtcgccaat 
      tgtcgtctg gaagatgaa agcaccgga aaagaaacc 
      gctagtaag acctgaaaa gcctgaaga agttcgggc 
      taaagtccg taaagggtc gcaaaagaa ggtgcagaa 
      tcctataca tgcgctttc tgaattcca aagcccaac 
      agaatcaat ttaagtatt ttttgtaaa atttataca 
      tttttatga atttctttt tccaatttt tgccgtaaa 
      agatcattt ttttatttt ttgttattt attaatgtt 
      acgtcgttg ccaacaaga atttatgta tttatgtgg 
      gtaggagca ctatttgca ctgtgggaa atataagca 
      atcatgaaa agttttaca tgtttgtaa ccattctat 
      ttttgttat ttttctaat gataaatgt tctgaaaat 
      taataaaga aagtgtggc ttccaatat ttggatatg 
      cttaagtta attctaagt ttactttgc aagaactat 
      tacaacttg cgtgcttac ataagtggt aataaacat 
      catttttac at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY