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Gene information
General information
TC004597 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 6615487..6627460
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004597
    iBeetle screen iB_00730
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    Adult morphology
    motility decreased - (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004597 RA
      MVESEPPSK NKQDCDVKD ERVVNGCGS KDLELANLA 
      TLKDLNKLP VDVEKGSSD KADFERAIE LTGYGKFHY 
      ILLAVCGFV STSEEMDVI SMSFILPSA QCDLNLSTQ 
      SKGWLNSII FIGMMAGAY AWGSVADAL GRKKVLIAI 
      SFMNALCIV ASSFSQNYE LFMLFRFLN GAALGGSGP 
      VIWSYFAEF QPKSKRGSM LSFMAAFWT LGNLFVAGL 
      AWLVIPTGI GFYYESFTY NSWRIFLLI CAIPSLIVA 
      ILLFFLPES PKFLLSRGR SEEAIEIFK IIYSTNTGR 
      DKEEYPVKQ LILDELLEQ QLIDTKTPL KGKYKNMIS 
      DIIENSKLL FVTPILKFT VISITINFT FHIGYYGLM 
      MWFPELFNR FDEYAVKHP NQSASVCEV TSFVTEHGS 
      QQDGSICSD EIGSSVFLE SLITVASAI PSNIIAVLF 
      MDLLGRKFF LVFSTVTSG LCAASMYFV YNKLHNLVV 
      SAVFSSVIS CGNAALDCL ITEIFPTNL RATGVAVSM 
      VAARLGGII GNLVIANLL DKYCPAPTF IVAALLIGG 
      GLLCLFLPN TTREPLS
    CDS sequence
    • >TC004597 RA
      atggtagaa agtgaacct ccttcaaaa aacaagcaa 
      gattgtgat gtaaaggac gagcgcgtt gtaaacggc 
      tgcggatcc aaagatctt gagcttgca aacctcgcg 
      acactcaaa gacctcaac aaactccca gtggatgtc 
      gagaaggga tcatccgac aaagctgac ttcgagcgc 
      gccatcgag ctaaccgga tatggaaaa tttcactac 
      attctatta gcagtatgc ggcttcgtg agcacctcg 
      gaggagatg gacgtcatc tcgatgtcg ttcattctc 
      ccctcagcg caatgcgac ttaaacctc agcacccag 
      agcaagggc tggctcaac tccatcatc ttcatcggg 
      atgatggca ggggcctac gcctggggc tcggtggcc 
      gacgcgctg ggccgtaag aaggtgctc atcgcgatc 
      tctttcatg aacgctctg tgcatcgtg gcttcgagc 
      ttcagccag aactatgag ctgttcatg cttttccga 
      tttctaaat ggggcggca ctagggggc agcggacca 
      gtcatctgg tcgtacttt gcagaattc cagcctaag 
      tccaaaagg ggctccatg ttgtccttc atggcggca 
      ttctggact ttgggcaac ttattcgtg gcaggcctg 
      gcctggctc gttatacca acaggtatt ggcttctat 
      tacgaaagc tttacctac aactcctgg cggatattt 
      ctcctgatt tgtgcaata ccatcacta attgtcgca 
      atcttactc ttcttcttg cccgaaagt ccgaaattt 
      ctgctctca cgggggcga agcgaggaa gcaatcgaa 
      attttcaaa atcatctat tcgaccaac acaggacgg 
      gacaaggag gaatatcca gtcaagcaa cttatactt 
      gatgaacta ttagaacaa caactcatc gataccaaa 
      acgcccctc aaggggaaa tacaaaaac atgataagt 
      gatattata gaaaatagc aagctactt ttcgtcaca 
      cctatactc aagttcacc gtcatttcg atcacaatc 
      aacttcact ttccacatt ggttattac ggtttgatg 
      atgtggttc cccgaactg ttcaaccgt tttgacgaa 
      tacgccgtg aaacacccg aaccagagc gcctccgtc 
      tgcgaagtc acctctttc gtcaccgag cacgggagc 
      caacaagac ggaagcatc tgttcggac gaaattgga 
      agttcggtc tttttggag tctctgatc acagttgcg 
      tccgcaatc ccaagcaat ataatagca gtgctgttt 
      atggatctt ctcggccgt aaatttttc ttagtattc 
      agcacagtg acctccggg ctttgtgca gcttcgatg 
      tatttcgtt tacaacaaa ctccacaat ttggtcgtc 
      tcagcggtg ttcagcagc gtgatatcc tgcggtaac 
      gcagctctg gattgcctt atcactgaa atattcccg 
      acgaatctc agggctacc ggtgtggct gtttcaatg 
      gtggcagct cgacttggt ggtataatt ggtaattta 
      gttattgct aacttacta gataagtat tgtcccgca 
      ccaacattt atcgtagca gcattattg ataggggga 
      ggccttctc tgcctcttt ttgccaaac acaacacgt 
      gaaccttta tcttaa
    mRNA sequence
    • >TC004597 RA
      ctgttttta ccaacttag tttaactgc ggaggttgc 
      tagcagccc tgcaggagt gccaaaccc atctctgag 
      gacttcaga gctttcgtt cggttttcc gtccgctgc 
      ccccagtag aagccgccc ccggccgtg tccgccttc 
      gttcgcccg ccgcagaaa ccttctaac tcccccgac 
      agcatagaa tggtagaaa gtgaacctc cttcaaaaa 
      acaagcaag attgtgatg taaaggacg agcgcgttg 
      taaacggct gcggatcca aagatcttg agcttgcaa 
      acctcgcga cactcaaag acctcaaca aactcccag 
      tggatgtcg agaagggat catccgaca aagctgact 
      tcgagcgcg ccatcgagc taaccggat atggaaaat 
      ttcactaca ttctattag cagtatgcg gcttcgtga 
      gcacctcgg aggagatgg acgtcatct cgatgtcgt 
      tcattctcc cctcagcgc aatgcgact taaacctca 
      gcacccaga gcaagggct ggctcaact ccatcatct 
      tcatcggga tgatggcag gggcctacg cctggggct 
      cggtggccg acgcgctgg gccgtaaga aggtgctca 
      tcgcgatct ctttcatga acgctctgt gcatcgtgg 
      cttcgagct tcagccaga actatgagc tgttcatgc 
      ttttccgat ttctaaatg gggcggcac tagggggca 
      gcggaccag tcatctggt cgtactttg cagaattcc 
      agcctaagt ccaaaaggg gctccatgt tgtccttca 
      tggcggcat tctggactt tgggcaact tattcgtgg 
      caggcctgg cctggctcg ttataccaa caggtattg 
      gcttctatt acgaaagct ttacctaca actcctggc 
      ggatatttc tcctgattt gtgcaatac catcactaa 
      ttgtcgcaa tcttactct tcttcttgc ccgaaagtc 
      cgaaatttc tgctctcac gggggcgaa gcgaggaag 
      caatcgaaa ttttcaaaa tcatctatt cgaccaaca 
      caggacggg acaaggagg aatatccag tcaagcaac 
      ttatacttg atgaactat tagaacaac aactcatcg 
      ataccaaaa cgcccctca aggggaaat acaaaaaca 
      tgataagtg atattatag aaaatagca agctacttt 
      tcgtcacac ctatactca agttcaccg tcatttcga 
      tcacaatca acttcactt tccacattg gttattacg 
      gtttgatga tgtggttcc ccgaactgt tcaaccgtt 
      ttgacgaat acgccgtga aacacccga accagagcg 
      cctccgtct gcgaagtca cctctttcg tcaccgagc 
      acgggagcc aacaagacg gaagcatct gttcggacg 
      aaattggaa gttcggtct ttttggagt ctctgatca 
      cagttgcgt ccgcaatcc caagcaata taatagcag 
      tgctgttta tggatcttc tcggccgta aatttttct 
      tagtattca gcacagtga cctccgggc tttgtgcag 
      cttcgatgt atttcgttt acaacaaac tccacaatt 
      tggtcgtct cagcggtgt tcagcagcg tgatatcct 
      gcggtaacg cagctctgg attgcctta tcactgaaa 
      tattcccga cgaatctca gggctaccg gtgtggctg 
      tttcaatgg tggcagctc gacttggtg gtataattg 
      gtaatttag ttattgcta acttactag ataagtatt 
      gtcccgcac caacattta tcgtagcag cattattga 
      tagggggag gccttctct gcctctttt tgccaaaca 
      caacacgtg aacctttat cttaacaca caatatccc 
      aacttagtt caatcgcag actagtttg ttcgtatgt 
      taattattg ccattgtag gttcagagt aggtattgt 
      ctgtcatat tatgttagt attatagat tcttagatt 
      gttagattc atgtcttac tcatatttg caatgtaag 
      taaaagaaa taaaaaaca gcaaaacaa