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Gene information
General information
TC004590 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7082714..7086380
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004590
    iBeetle screen iB_00729

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection



    elytra partially blistered - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004590 RA
      MSVPDKDEL VQRAKLAEQ AERYDDMAS 
      AMKSVTETG VELSNEERN LLSVAYKNV 
      VGARRSSWR VISSIEQKT EGSERKQQM 
      AKEYREKVE KELREICDD VLRATLIKS 
      FLLENEGRL LPVFGRVAT GDTRNAVVD 
      YSQKAYQDA FEISKAKMT PTHPIRLGL 
      ALNFSVFYY EILNSPDKA CQLAKQAFD 
      DAIAELDTL NEDSYKDST LIMQLLRDN 
      LTLWTSDTQ GEADEPQET GDN
    CDS sequence
    • >TC004590 RA
      atgtctgtc cctgacaaa gatgagcta gtgcagagg 
      gcaaaactc gcagaacag gccgaaagg tacgatgac 
      atggcttcg gctatgaaa tcagtcaca gaaaccgga 
      gtagaattg agcaatgaa gaacgaaac ctcctttct 
      gtcgcgtac aagaatgtc gttggtgcc aggaggtca 
      tcgtggaga gttatttct tcaattgaa caaaagact 
      gagggctca gaacgcaaa cagcagatg gccaaagaa 
      taccgagaa aaggtcgaa aaggaacta agggagatc 
      tgtgacgac gtcctccga gcaacgctg atcaaaagt 
      tttttactt gaaaatgaa gggagatta ctaccggta 
      tttggccga gtagccaca ggagacaca agaaacgcc 
      gtcgtcgat tattcacaa aaggcatac caagacgct 
      tttgaaatc tcaaaggca aaaatgacg cccacacat 
      cccatcaga ttaggtctt gccctaaac ttctccgtt 
      ttctattat gagatatta aattcacca gacaaggct 
      tgtcagtta gctaaacag gccttcgat gacgcaata 
      gcagaactc gatacctta aatgaagac tcttacaag 
      gacagcact ctcataatg cagctcctc cgagacaac 
      ctcacactc tggacaagt gacacgcag ggcgaagcg 
      gatgagcct caagagact ggtgataat tga
    mRNA sequence
    • >TC004590 RA
      gtaggatcg ttccatttt tctatcaaa aaatcgtat 
      tcaaccctt gtagcacat ttagaatta gagcaaaat 
      gtctgtccc tgacaaaga tgagctagt gcagagggc 
      aaaactcgc agaacaggc cgaaaggta cgatgacat 
      ggcttcggc tatgaaatc agtcacaga aaccggagt 
      agaattgag caatgaaga acgaaacct cctttctgt 
      cgcgtacaa gaatgtcgt tggtgccag gaggtcatc 
      gtggagagt tatttcttc aattgaaca aaagactga 
      gggctcaga acgcaaaca gcagatggc caaagaata 
      ccgagaaaa ggtcgaaaa ggaactaag ggagatctg 
      tgacgacgt cctccgagc aacgctgat caaaagttt 
      tttacttga aaatgaagg gagattact accggtatt 
      tggccgagt agccacagg agacacaag aaacgccgt 
      cgtcgatta ttcacaaaa ggcatacca agacgcttt 
      tgaaatctc aaaggcaaa aatgacgcc cacacatcc 
      catcagatt aggtcttgc cctaaactt ctccgtttt 
      ctattatga gatattaaa ttcaccaga caaggcttg 
      tcagttagc taaacaggc cttcgatga cgcaatagc 
      agaactcga taccttaaa tgaagactc ttacaagga 
      cagcactct cataatgca gctcctccg agacaacct 
      cacactctg gacaagtga cacgcaggg cgaagcgga 
      tgagcctca agagactgg tgataattg aagtgtggc 
      gtccgtttt aaaattatt cgttaaaaa tgaagtttc 
      atgtaagtt aacgtattc gtggaactt ggttgtgaa 
      atttcattg acagtttta agcaaagtt tcacctgat 
      gaaacttga tatttaaca atgttttta ggagatgtg 
      taaatcgtt aaaatttaa ttacatttt cacacaacc 
      aaaacaatg tcagccaca attaagttt ttaattcaa 
      aaaatcatc attgattca aatttaccg gaagttgtg 
      tggttcagt gtattttgg cattttctc atttttgct 
      cttatgttt tttctcctt atttttgta attagtgtt 
      tcatttttc cataagtgt aatttattt gaaatttca 
      gtaattttt aatttctaa tttgttaac tattaaaat 
      tattattta ataacactg ttttagtta tattttttg 
      tattcgtcc attgttgaa atctccaaa aataaatca 
      tacctatac ctctttata cattaaaaa agcctgtac 
      cttagtcca caaaattat tttgcactc cgctgcatg 
      ggccgtatc tgccttccc ccccttgtt taccgtcaa 
      ctcactttg gatgtgcta tatataaat gtatgaata 
      tgtattaaa ataaactgc tatcagctc tacttgttt 
      tctgttgta tctggcaaa tatgtatat ttgtctgat 
      atcaataaa tttttcaaa atctatttt gttttattt 
      atattatat tactaggtg tttttcgat tgattggat 
      tgattgtca aagtgcgga cgtaactac atgtactat 
      tatgttctc tcttaattg ctatgttac gtgttgtat 
      taagaaatg tagtaaaaa aggtgacat gtaaaatcg 
      gtcagattc tagctgata atggttgtt ggaggggac 
      agcagaata ctgtcttga cttgaggct tgcataagt 
      cacattttc gctgactta tggtggtcc aaattttta 
      tttttgttt ttcagattt aatatagtt acattttaa 
      gcctgtaaa aatgtcatc aaataaaat tctttgaaa 
      cga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY