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Gene information
General information
TC004585 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 6932695..6954768
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004585
    iBeetle screen iB_00728
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature partially ap-orientation not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature dorsal shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004585 RA
      MSSYSQSYR TANDNGYIW DGSMCNCCP YGYHIDLDF 
      VRYCETISQ EADRTGSIK RRKDRRRQR QSMEVLLGI 
      TPPVLAALE QSYKTIQEQ ETPKISQTS SQIPVSQDA 
      FDDAISDFE RTLQRSKNK LYNNLPDVT AVSETIKRV 
      PSNSSMSSV SGSSMTVLP ETTNRDFDT VSIESCGLS 
      PLALQSIRE QMAISLERT KQLEDQVKL IPQLKEQLS 
      SLREENRRL HLQLKSDET QKYNTINAN YLRQSPQNN 
      RLKSQSFTN IDTLDSKEA PPPPPRKDF GVNCGVLTR 
      NIGVGHQNP NTKNVSTAT LDDLTVDKW FNEKIKFMN 
      SQNLSKNIS RSSQTLLKE IRGVASQTT EKPTIETHT 
      QTPETKKYY SHASVSAVP KNRDFSVQK TVEVYNIGT 
      SDDTISDII CEKCSVCKC SVGVGPDSS EDKHTSPVS 
      LASLMGPRS KSFNLGEAP LNFSSRNRT VACQYESFG 
      VSKASQYEA TGVGKACQC EVKTHSKAC QHEAKQLHK 
      NTQYESNSV SKDTDTKDL IAACEAKER KKLADKGSN 
      TCDDKTVCA KCTNREKDE VGKKDTSFM SKIPRPQIP 
      TTPVENRKF RRQDTYTKI YSSPGSPTQ GLSGLDLSG 
      KSPSNELSN PIDKLTHNF KLGEKSHAQ NGVAAPTKV 
      QIQESTLPL PETALFNPA SAQQHRKKA VPSKGMQGA 
      MKVLNDSLQ KSQTKNPKN HNAVNIVQK EWFQISSLY 
      TANPLDVED YLDAFEEVS SVLLQYIVN MTDESGNTA 
      MHYAVSHGN FDVVSILLD SKVCDINKP NKAGYTSVM 
      LVSLAEVRS QTHANVVRR LFQLADVNI RAKQHGQTA 
      LMLAVSHGR LDMVKMLLE AGADINIQD EDGSTALMC 
      AAEHGHVEI VKHFLNQSD CDSSITDID GSTALKIAM 
      EAGHRHIGV LLYAHQRNL QMIHGKKKS KPASPKTPS 
      SPLPIRSSH RALTDTKTT K
    CDS sequence
    • >TC004585 RA
      atgtcatca tactcgcaa agctatagg actgcaaac 
      gacaatggg tacatctgg gacggcagt atgtgtaat 
      tgctgccct tatggctac cacatcgat ttggacttc 
      gtccgctac tgcgaaaca atcagccaa gaggctgac 
      agaacggga tcgatcaaa cgacgaaaa gatcgacga 
      cgacaacga caatcgatg gaagttttg ttgggaatt 
      acgcccccg gttctggcc gctttagag caaagttat 
      aaaacgatt caagaacag gaaactccg aaaataagc 
      cagactagc agccagatt cccgtaagt caagatgcg 
      tttgatgat gccatctcg gattttgaa aggactttg 
      caacgttcc aaaaacaaa ttgtacaat aatttacca 
      gatgttaca gccgtttca gaaaccatc aaaagggtg 
      ccatcgaat tcttcgatg tcctcggtc tccggttcg 
      agtatgact gtgctgcca gaaacaaca aatcgcgat 
      ttcgatacg gtcagtata gagtcatgc ggcttgagc 
      cctttggcc ctacaaagc ataagggaa cagatggcc 
      atcagctta gaaagaacc aaacagctt gaagaccaa 
      gtcaaactt ataccacaa ctcaaggag caattatcc 
      tcgttaagg gaggaaaac cgccgtctg cacctccaa 
      ctaaaatcc gatgaaacg caaaaatac aacacaata 
      aacgcaaat tatctccgg caatccccc cagaacaat 
      cgattgaaa tcgcaatcg tttaccaac attgacacc 
      ctcgactca aaggaggcg cctccaccc ccgccccga 
      aaagacttc ggggtgaat tgtggcgtc ctgacccga 
      aatatcggc gtcggccac caaaacccc aacactaaa 
      aacgtctcg acggcaact ctggacgac ctcacagtg 
      gataaatgg ttcaacgag aaaatcaaa ttcatgaac 
      agtcaaaat ttatccaaa aacatctcg cgatcgtcc 
      caaactctt ctcaaagaa atccgaggt gtcgctagt 
      caaacaacc gaaaaacca accatcgag acacacaca 
      caaaccccc gaaaccaag aagtattac tcgcacgct 
      agcgtgtcg gcagtcccg aaaaatcgc gattttagc 
      gtgcaaaaa accgtggaa gtttacaac atcgggacg 
      tctgacgac acgatcagt gatataatt tgcgaaaaa 
      tgcagcgtt tgtaaatgc agtgtgggg gtggggccg 
      gacagcagc gaagacaag cacacgagc cccgtctcg 
      ctggcgtcc ctaatgggg cccaggagc aagtcgttc 
      aatttgggc gaagccccg ctgaatttc agctcgagg 
      aatcggaca gtggcgtgc cagtacgaa agcttcggg 
      gtctctaaa gcctcacag tacgaagct acgggggtt 
      gggaaggcc tgccagtgc gaagtgaaa acgcactca 
      aaagcgtgc caacacgaa gccaaacaa ctccacaag 
      aacacgcag tatgaaagc aattccgtc tcgaaagac 
      accgatacc aaagatctg atcgctgct tgcgaagcc 
      aaagaacgc aagaaattg gccgacaaa gggtccaac 
      acttgcgat gataaaaca gtatgtgcg aagtgtaca 
      aatagggag aaggacgaa gtcggtaag aaggatacg 
      agttttatg tcgaaaatt ccaaggccc cagataccg 
      accactccg gtggaaaat aggaagttt agaagacag 
      gacacttac acgaagatt tattcatcg ccagggtca 
      ccgactcaa ggattatca ggactcgac ttgtccgga 
      aaatcccca tcgaacgaa ctctcaaat cccattgat 
      aaactcacc cacaatttc aaattaggg gaaaaatct 
      catgctcaa aatggcgtt gccgccccc acaaaagtg 
      caaatccaa gaatcaaca ctgccacta cccgaaacg 
      gcgctattc aaccctgct agtgctcaa caacacagg 
      aaaaaagcg gtgccaagc aaaggaatg caaggtgcg 
      atgaaggtc ctgaacgac tccctccaa aaaagtcaa 
      acgaaaaat cccaaaaac cacaacgcg gttaatatc 
      gtccagaaa gagtggttc cagatatca agcttgtat 
      acggcgaat cccctagat gtcgaggac tatttagac 
      gcttttgaa gaagtctct tcggttttg ctccaatat 
      attgttaat atgactgat gaaagtggc aacactgcg 
      atgcattac gcagtatcg cacgggaat ttcgacgtt 
      gtgtccatt ctacttgac tcaaaagtt tgtgatatt 
      aataaaccc aacaaggct ggctacacg agcgtcatg 
      ctggtatca ctggccgag gtcaggtca cagacgcac 
      gccaacgtc gtacgaagg ctattccag ctcgctgac 
      gtcaacata cgcgcaaaa cagcacggg cagacggcc 
      ttaatgttg gcggtgagt catggccgg ctcgatatg 
      gtgaaaatg ttgctcgaa gcaggcgct gatattaac 
      atccaagac gaggatggc agcactgct ctgatgtgc 
      gcagccgag cacggccat gttgaaata gtgaaacac 
      tttttgaac caatcagat tgcgactcc agtattacc 
      gatatcgat ggtagcact gcgctcaaa attgcaatg 
      gaggctgga cacagacat atcggagtt ttgctctac 
      gctcatcag agaaacctc caaatgata cacgggaaa 
      aagaaatcg aaaccggca agccccaaa accccgtct 
      tcgcccttg cctatacgg agcagtcat cgtgcctta 
      accgatact aaaactact aagtag
    mRNA sequence
    • >TC004585 RA
      ctagaatgc gttggtcat tgtgaagtt atctttctt 
      agaagaagt gtggaatag tgtgtatgt tgacaattt 
      cggaaagca acaagcaaa agatgtgat ttcgtgtaa 
      attctaaat gaaaagtgc gtggaaacg cgacgatgt 
      atttaaatt ttttcaagt gaaagttga gcggcttga 
      attggcggt tttttgtgt cgtttttga aaagttctt 
      tggaatttg ttgtgcaaa ttctggaag ataactgac 
      aatgtcatc atactcgca aagctatag gactgcaaa 
      cgacaatgg gtacatctg ggacggcag tatgtgtaa 
      ttgctgccc ttatggcta ccacatcga tttggactt 
      cgtccgcta ctgcgaaac aatcagcca agaggctga 
      cagaacggg atcgatcaa acgacgaaa agatcgacg 
      acgacaacg acaatcgat ggaagtttt gttgggaat 
      tacgccccc ggttctggc cgctttaga gcaaagtta 
      taaaacgat tcaagaaca ggaaactcc gaaaataag 
      ccagactag cagccagat tcccgtaag tcaagatgc 
      gtttgatga tgccatctc ggattttga aaggacttt 
      gcaacgttc caaaaacaa attgtacaa taatttacc 
      agatgttac agccgtttc agaaaccat caaaagggt 
      gccatcgaa ttcttcgat gtcctcggt ctccggttc 
      gagtatgac tgtgctgcc agaaacaac aaatcgcga 
      tttcgatac ggtcagtat agagtcatg cggcttgag 
      ccctttggc cctacaaag cataaggga acagatggc 
      catcagctt agaaagaac caaacagct tgaagacca 
      agtcaaact tataccaca actcaagga gcaattatc 
      ctcgttaag ggaggaaaa ccgccgtct gcacctcca 
      actaaaatc cgatgaaac gcaaaaata caacacaat 
      aaacgcaaa ttatctccg gcaatcccc ccagaacaa 
      tcgattgaa atcgcaatc gtttaccaa cattgacac 
      cctcgactc aaaggaggc gcctccacc cccgccccg 
      aaaagactt cggggtgaa ttgtggcgt cctgacccg 
      aaatatcgg cgtcggcca ccaaaaccc caacactaa 
      aaacgtctc gacggcaac tctggacga cctcacagt 
      ggataaatg gttcaacga gaaaatcaa attcatgaa 
      cagtcaaaa tttatccaa aaacatctc gcgatcgtc 
      ccaaactct tctcaaaga aatccgagg tgtcgctag 
      tcaaacaac cgaaaaacc aaccatcga gacacacac 
      acaaacccc cgaaaccaa gaagtatta ctcgcacgc 
      tagcgtgtc ggcagtccc gaaaaatcg cgattttag 
      cgtgcaaaa aaccgtgga agtttacaa catcgggac 
      gtctgacga cacgatcag tgatataat ttgcgaaaa 
      atgcagcgt ttgtaaatg cagtgtggg ggtggggcc 
      ggacagcag cgaagacaa gcacacgag ccccgtctc 
      gctggcgtc cctaatggg gcccaggag caagtcgtt 
      caatttggg cgaagcccc gctgaattt cagctcgag 
      gaatcggac agtggcgtg ccagtacga aagcttcgg 
      ggtctctaa agcctcaca gtacgaagc tacgggggt 
      tgggaaggc ctgccagtg cgaagtgaa aacgcactc 
      aaaagcgtg ccaacacga agccaaaca actccacaa 
      gaacacgca gtatgaaag caattccgt ctcgaaaga 
      caccgatac caaagatct gatcgctgc ttgcgaagc 
      caaagaacg caagaaatt ggccgacaa agggtccaa 
      cacttgcga tgataaaac agtatgtgc gaagtgtac 
      aaataggga gaaggacga agtcggtaa gaaggatac 
      gagttttat gtcgaaaat tccaaggcc ccagatacc 
      gaccactcc ggtggaaaa taggaagtt tagaagaca 
      ggacactta cacgaagat ttattcatc gccagggtc 
      accgactca aggattatc aggactcga cttgtccgg 
      aaaatcccc atcgaacga actctcaaa tcccattga 
      taaactcac ccacaattt caaattagg ggaaaaatc 
      tcatgctca aaatggcgt tgccgcccc cacaaaagt 
      gcaaatcca agaatcaac actgccact acccgaaac 
      ggcgctatt caaccctgc tagtgctca acaacacag 
      gaaaaaagc ggtgccaag caaaggaat gcaaggtgc 
      gatgaaggt cctgaacga ctccctcca aaaaagtca 
      aacgaaaaa tcccaaaaa ccacaacgc ggttaatat 
      cgtccagaa agagtggtt ccagatatc aagcttgta 
      tacggcgaa tcccctaga tgtcgagga ctatttaga 
      cgcttttga agaagtctc ttcggtttt gctccaata 
      tattgttaa tatgactga tgaaagtgg caacactgc 
      gatgcatta cgcagtatc gcacgggaa tttcgacgt 
      tgtgtccat tctacttga ctcaaaagt ttgtgatat 
      taataaacc caacaaggc tggctacac gagcgtcat 
      gctggtatc actggccga ggtcaggtc acagacgca 
      cgccaacgt cgtacgaag gctattcca gctcgctga 
      cgtcaacat acgcgcaaa acagcacgg gcagacggc 
      cttaatgtt ggcggtgag tcatggccg gctcgatat 
      ggtgaaaat gttgctcga agcaggcgc tgatattaa 
      catccaaga cgaggatgg cagcactgc tctgatgtg 
      cgcagccga gcacggcca tgttgaaat agtgaaaca 
      ctttttgaa ccaatcaga ttgcgactc cagtattac 
      cgatatcga tggtagcac tgcgctcaa aattgcaat 
      ggaggctgg acacagaca tatcggagt tttgctcta 
      cgctcatca gagaaacct ccaaatgat acacgggaa 
      aaagaaatc gaaaccggc aagccccaa aaccccgtc 
      ttcgccctt gcctatacg gagcagtca tcgtgcctt 
      aaccgatac taaaactac taagtagtg taacttgtt 
      attaaatgt tttaatagt tgttaaagg tgtgtcata 
      actagtttt atctggtag acgaataat gtttgttgt 
      agttgcgta gggaccaat tacccgatc actaattta 
      attaattac attccagtt ttttaattt aattctttt 
      gtattatat ttgtatatt tgtaattaa tagactcac 
      ctctaataa acataactg cgtttt