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Gene information
General information
TC004581 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7348060..7354420
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004581
    iBeetle screen iB_00726

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004581 RA
      MALRTWEVE VFNVTEDIV CTPKYNKKV KLRADYDPQ 
      GISIKLLDG DDSHVLSEF PISSSSECC RVGPKSYIF 
      SFDNEALLF KFGSIEDAR SFTIAITKV KSGKGASVF 
      SVRTEDSSA TQYFQFYGY LSQQQNMLQ DFVRTYTYQ 
      RAILCNLDD FKDKVILDV GAGSGILSF FAAQAGAKR 
      IYAVEASTM AHFAQKLVD ANNLSDSIK VIPGKIEEI 
      ELPEKVDVI ISEPMGYML YNERMLETY LHAKKWLLP 
      GGKMYPSRG DLHIAPFTD DSLYMEQYS KANFWFQTC 
      FHGVNLSAI QNCAVKEYF RQPIVDTFD IRICMSKSI 
      RHVVDFSEA HETDLHTID IPLEFHILE SGTCHGLAF 
      WFDVAFAGS QQTIWLSTG PTEPLTHWY QVRCLLEQP 
      LLLKQGQVL TGRVLLVAN KRQSYDVTI DLAVEGTNQ 
      SSKNTLDLK NPYFRYTGA PVQPPPGIS NVSPSENYW 
      SQLDAQGVR NAVNMVNGM SVNGLGEVS MDTTQTIIN 
      SNMMANNLI ALVDPSHLS EPTQPNIHP GCISSTGRG 
      RVAASPTST HTAQLIGGA ISPSLFTSP NPQQQQPLV 
      MTNYPVSAN LMIGDYVTP GNGVAMPAY RQ
    CDS sequence
    • >TC004581 RA
      atggcgctt aggacctgg gaagttgaa gtttttaat 
      gtgacagaa gacattgtt tgcactcca aaatataac 
      aaaaaagtg aaacttaga gccgattat gatccacaa 
      ggaattagc attaaatta ctcgacggt gacgactct 
      cacgtttta tcagaattt cccattagt tcgtcttcg 
      gagtgctgc cgcgtgggg cctaaatcc tacatattt 
      tcctttgat aacgaagcc ctgttgttc aagttcggc 
      tccattgaa gacgcgagg agctttact attgctata 
      acaaaggtc aagtctggg aaaggtgct tcggttttt 
      tcggttagg accgaagac tcgtccgct actcagtat 
      ttccagttt tatggctac ttgtcacag caacaaaac 
      atgttacaa gactttgtt aggacgtat acgtatcaa 
      agagcaata ttatgtaac ttagacgat tttaaggat 
      aaagtcatt ttggatgtt ggtgccggt tctggcatt 
      ctatcattt tttgctgca caagctggc gctaagagg 
      atttacgcc gtcgaagcg tccacaatg gcacacttt 
      gcgcagaaa ttggtggat gctaacaat ttgtcagac 
      tcaattaaa gtaattccg ggtaaaata gaagaaatt 
      gaacttcca gaaaaagta gatgtaatt atttccgaa 
      ccaatgggt tacatgctt tacaacgag agaatgttg 
      gagacttac ttgcacgcg aaaaagtgg ctgttgcca 
      ggcgggaaa atgtacccc tcccggggg gacctgcac 
      atagcgccg ttcaccgac gattccctc tacatggaa 
      cagtacagc aaagcgaat ttttggttt caaacgtgt 
      ttccatggg gttaacttg tccgcgatt caaaactgc 
      gccgtgaag gagtacttt agacaacca attgttgat 
      actttcgat attagaatt tgcatgagc aagtcgatt 
      agacacgtt gtagatttt tcggaagct catgaaacc 
      gatcttcac actatagat attcccttg gagttccat 
      attttagaa tcggggact tgtcacggc ttggcattt 
      tggtttgat gtagctttt gccggttcg caacaaacc 
      atatggctt agtaccggt ccaaccgag cctttaacg 
      cactggtac caggttagg tgcttacta gaacagccg 
      cttttactt aaacaaggc caggtttta accggtcga 
      gtactactc gtagctaat aaaagacaa agttacgac 
      gttactata gacttagca gttgaagga acaaaccaa 
      agttcgaaa aatacccta gatttgaaa aatccatac 
      tttagatat accggagcg ccagtacaa ccccctccc 
      ggaatttca aacgtgtca ccaagtgag aactactgg 
      agtcaattg gatgctcaa ggtgttagg aatgctgtg 
      aatatggtg aacgggatg tcggtgaac ggcttgggc 
      gaagtgtca atggatacc acgcagacc atcattaac 
      agcaacatg atggccaac aatctcata gctttagtg 
      gatccttct catttatca gaaccgact cagcccaat 
      atccatcca ggttgtatt tccagtacc ggcagaggg 
      cgcgttgct gctagtcca acgtcaact cacacagct 
      cagttaatt ggtggagcg atttcgccg tcgttattc 
      acctcacct aatccacaa cagcaacaa cctctagtg 
      atgacaaat tacccggtt agtgctaat ttaatgatc 
      ggagactat gttaccccc ggaaatgga gtagcaatg 
      cctgcatat cgccagtag
    mRNA sequence
    • >TC004581 RA
      gttgttgtt acaaatgcg agtgcggta gtgtgactg 
      gcgaaaaat ggcgcttag gacctggga agttgaagt 
      ttttaatgt gacagaaga cattgtttg cactccaaa 
      atataacaa aaaagtgaa acttagagc cgattatga 
      tccacaagg aattagcat taaattact cgacggtga 
      cgactctca cgttttatc agaatttcc cattagttc 
      gtcttcgga gtgctgccg cgtggggcc taaatccta 
      catattttc ctttgataa cgaagccct gttgttcaa 
      gttcggctc cattgaaga cgcgaggag ctttactat 
      tgctataac aaaggtcaa gtctgggaa aggtgcttc 
      ggttttttc ggttaggac cgaagactc gtccgctac 
      tcagtattt ccagtttta tggctactt gtcacagca 
      acaaaacat gttacaaga ctttgttag gacgtatac 
      gtatcaaag agcaatatt atgtaactt agacgattt 
      taaggataa agtcatttt ggatgttgg tgccggttc 
      tggcattct atcattttt tgctgcaca agctggcgc 
      taagaggat ttacgccgt cgaagcgtc cacaatggc 
      acactttgc gcagaaatt ggtggatgc taacaattt 
      gtcagactc aattaaagt aattccggg taaaataga 
      agaaattga acttccaga aaaagtaga tgtaattat 
      ttccgaacc aatgggtta catgcttta caacgagag 
      aatgttgga gacttactt gcacgcgaa aaagtggct 
      gttgccagg cgggaaaat gtacccctc ccgggggga 
      cctgcacat agcgccgtt caccgacga ttccctcta 
      catggaaca gtacagcaa agcgaattt ttggtttca 
      aacgtgttt ccatggggt taacttgtc cgcgattca 
      aaactgcgc cgtgaagga gtactttag acaaccaat 
      tgttgatac tttcgatat tagaatttg catgagcaa 
      gtcgattag acacgttgt agatttttc ggaagctca 
      tgaaaccga tcttcacac tatagatat tcccttgga 
      gttccatat tttagaatc ggggacttg tcacggctt 
      ggcattttg gtttgatgt agcttttgc cggttcgca 
      acaaaccat atggcttag taccggtcc aaccgagcc 
      tttaacgca ctggtacca ggttaggtg cttactaga 
      acagccgct tttacttaa acaaggcca ggttttaac 
      cggtcgagt actactcgt agctaataa aagacaaag 
      ttacgacgt tactataga cttagcagt tgaaggaac 
      aaaccaaag ttcgaaaaa taccctaga tttgaaaaa 
      tccatactt tagatatac cggagcgcc agtacaacc 
      ccctcccgg aatttcaaa cgtgtcacc aagtgagaa 
      ctactggag tcaattgga tgctcaagg tgttaggaa 
      tgctgtgaa tatggtgaa cgggatgtc ggtgaacgg 
      cttgggcga agtgtcaat ggataccac gcagaccat 
      cattaacag caacatgat ggccaacaa tctcatagc 
      tttagtgga tccttctca tttatcaga accgactca 
      gcccaatat ccatccagg ttgtatttc cagtaccgg 
      cagagggcg cgttgctgc tagtccaac gtcaactca 
      cacagctca gttaattgg tggagcgat ttcgccgtc 
      gttattcac ctcacctaa tccacaaca gcaacaacc 
      tctagtgat gacaaatta cccggttag tgctaattt 
      aatgatcgg agactatgt tacccccgg aaatggagt 
      agcaatgcc tgcatatcg ccagtagtg tgtaaatcg 
      gcactgtca tgctcaaaa actgttaaa aaattcgat 
      aatggtcgc agacaatta atttacatg tatatgttt 
      gatttaact actttattg tttcgaatc gtgtttgat 
      ttttgtgta tttttatta ttgctttat catgtgatt 
      tgtataaac aagtgcgga gatgttttt cgagatacg 
      cattttttg tttgcgaaa aaacatctt tctgggatt 
      tagtcccat gccttagct tatcatgaa tgcggcagg 
      gagcaactt tgtattaaa caatcgcga tttttagta 
      tttattatg tcatttagt tgaaattgt gcatcactt 
      aagcaataa attttatgc ctttttaaa atgttttct 
      gattcgttt cgtgttctt gcatcactc ttgtattac 
      aatgtgaca atagtaatt taattgtat acacttatc 
      tctatatag caacattgc atatctttc tttgacgtc 
      ctctgtcgt tcgacttag tttatttat gtttagtag 
      tgtagtgcg tttgtatga tctgtaaat ttttcaaat 
      ttagtttaa gaaacaggt atttttgga tatatttta 
      atgtcatat caacaaggc atgatgtat gacataatt 
      ggcaactgt tttacgaag tgaaactta ttttattgt 
      ggatgttgc tattttgtc tgtgatatt ttggttaaa 
      cacgtttcc tgaattaat tttattgta gatgtagga 
      aatacagga tagtttgtt cgcctaccg tgtttgtat 
      atttattct ttaatgtaa tctcttcat tttaaaacg 
      aatcccttg tgttgcgtt tatcaagtg catctgata 
      ttatatgca atgaagcag tcattgttt acgttaata 
      ggcaatgga gcatattct gccttacaa taccacgtg 
      caatggcag tgtaatgaa agcctagtt acatataat 
      aatgcttca tgtatacca taggataaa ttgatctgt 
      acattggat tcattaaga acaatatat tgtaccact 
      tttg
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