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Gene information
General information
TC004574 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2132542..2134656
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004574
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00724

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004574 RA
      MTTAGKPIT CKAAVAWGP PRRPKTRNH RICHTDAYT 
      LSGQDPEGL FPVILGHEG GGIVESVGE GVTNVQPGD 
      HVIPLYIPQ CGECKFCKS PKTNLCQKI RVTQGQGVM 
      PDGTSRFTC NGKKVYHFM GCSTFSEYT VVADISVAK 
      VNPNAELDK ICLLGCGVP TGYGAALNT AKVEKGSTC 
      AVWGLGAVG LAVGIGCKA AGASKIIGV DVNPDKFKI 
      AKDFGFTDF VNPKDYEKP IQEVLAEKT DGGLDFTFE 
      CVGNVQTMR AALESCHKG WGVSTIVGV AGAGQEIST 
      RPFQLVTGR VWKGTAFGG WKSRDSVPK LVEEYCHKP 
      QIMPLDKFI THNLKFGQI NEAFHLMHK GER
    CDS sequence
    • >TC004574 RA
      atgactact gccggaaag cctataact tgcaaagct 
      gcagttgct tgggggccc ccgagaaga cctaaaact 
      cgaaaccat cgaatttgt catacagat gcttacacc 
      ctcagtggc caagatccc gagggatta ttccctgtc 
      atcttaggc cacgagggc ggtggaatt gtggaaagt 
      gtgggagag ggagttaca aacgttcag ccaggtgac 
      catgtcatt ccgttatac attccccaa tgtggcgaa 
      tgcaaattt tgcaagtcg cccaaaacc aacttgtgc 
      caaaaaatc agggtgaca cagggacaa ggggtgatg 
      cccgatggt accagtaga ttcacgtgc aatggtaaa 
      aaagtgtac cattttatg ggttgctcc acctttagc 
      gagtatacc gttgttgcc gacatctca gttgctaaa 
      gtgaatccc aatgccgaa cttgacaaa atttgtctg 
      ttgggttgt ggcgtccca actggatat ggagcagcc 
      ttaaatacc gccaaagtt gaaaaaggg agcacctgc 
      gcggtttgg ggcttgggg gctgtgggc cttgccgtc 
      gggattgga tgcaaggct gcgggggcg tccaaaatc 
      atcggagtc gatgtcaat ccagacaaa ttcaaaatt 
      gccaaagac ttcggcttc accgacttc gtgaacccc 
      aaagactac gaaaagccg attcaggaa gtgttggct 
      gaaaagaca gacggtggt ctcgacttc actttcgaa 
      tgtgttggg aatgttcag actatgaga gcagcgttg 
      gagtcatgt cacaagggt tggggggtc tccacaatt 
      gtgggggtt gctggtgca gggcaagaa ataagcact 
      agacctttc cagctcgta acagggagg gtgtggaaa 
      ggaacggct tttggaggg tggaaaagc cgcgatagt 
      gttccgaaa ctagttgag gaatattgc cataaacct 
      caaatcatg ccgcttgac aagtttatc acgcataat 
      ttgaaattt ggtcaaatt aacgaagcg ttccatctt 
      atgcacaaa ggggaaagg taa
    mRNA sequence
    • >TC004574 RA
      aaccaatta tcaacaccc aaccttaac ctcacaagg 
      tgttttttt ccaccatga ctactgccg gaaagccta 
      taacttgca aagctgcag ttgcttggg ggcccccga 
      gaagaccta aaactcgaa accatcgaa tttgtcata 
      cagatgctt acaccctca gtggccaag atcccgagg 
      gattattcc ctgtcatct taggccacg agggcggtg 
      gaattgtgg aaagtgtgg gagagggag ttacaaacg 
      ttcagccag gtgaccatg tcattccgt tatacattc 
      cccaatgtg gcgaatgca aattttgca agtcgccca 
      aaaccaact tgtgccaaa aaatcaggg tgacacagg 
      gacaagggg tgatgcccg atggtacca gtagattca 
      cgtgcaatg gtaaaaaag tgtaccatt ttatgggtt 
      gctccacct ttagcgagt ataccgttg ttgccgaca 
      tctcagttg ctaaagtga atcccaatg ccgaacttg 
      acaaaattt gtctgttgg gttgtggcg tcccaactg 
      gatatggag cagccttaa ataccgcca aagttgaaa 
      aagggagca cctgcgcgg tttggggct tgggggctg 
      tgggccttg ccgtcggga ttggatgca aggctgcgg 
      gggcgtcca aaatcatcg gagtcgatg tcaatccag 
      acaaattca aaattgcca aagacttcg gcttcaccg 
      acttcgtga accccaaag actacgaaa agccgattc 
      aggaagtgt tggctgaaa agacagacg gtggtctcg 
      acttcactt tcgaatgtg ttgggaatg ttcagacta 
      tgagagcag cgttggagt catgtcaca agggttggg 
      gggtctcca caattgtgg gggttgctg gtgcagggc 
      aagaaataa gcactagac ctttccagc tcgtaacag 
      ggagggtgt ggaaaggaa cggcttttg gagggtgga 
      aaagccgcg atagtgttc cgaaactag ttgaggaat 
      attgccata aacctcaaa tcatgccgc ttgacaagt 
      ttatcacgc ataatttga aatttggtc aaattaacg 
      aagcgttcc atcttatgc acaaagggg aaaggtaag 
      ttcttttat tgtgtgata cttgac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY