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Gene information
General information
TC034543 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid6: 212348..249104
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034543
    iBeetle screen iB_00723
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034543 RA
      MRVLLVLVV AAAVVCTTD AKRKRKFDG DFEFAEEDE 
      SKAAKSGEK KRWIHDPSS DLCRPLNCK KKEICLLED 
      SFTAVCVSR KELKKNGDI VVPKSTVKT EESKNEDDD 
      DDDVFYDTE DDPDDTDED LNDSVVCKP CPVVKPTFL 
      CGSDNRTYS SLCRLDYHN CIHHTSIRV TCKGFCPCK 
      ENEAHLRKK QRQSERLNS FMNKYKATL DKSGTVGGS 
      INGAKPAQP SSKSDVYTF TPQDFKYEN KHYKYIKYT 
      KYNKDNNNI LYAEDKDRL RGYNEVTDN KLYSNSIGV 
      LDPAISPSK QCSPAALQA MGNRLLDWF SVVMADSSK 
      RRRPRNKSK AHFPSACKG EVRWMFQHL DANSDAKLS 
      LQELYDLEH DQSEVCLKP FLQQCDIDR DVIVTPLEW 
      CRCFQRTER PCAAVKRKI TPELLGVYV PDCDNQGYY 
      RPTQCHSAI GMCWCVDKH GVEFANTRT HAKPNCEAL 
      INKSNGVAK QTSPEDASD DEDGDDVEQ DIEGSADHP 
      GDY
    CDS sequence
    • >TC034543 RA
      atgcgagtg cttctggtc ctggtcgtc gcggcggcc 
      gtcgtgtgc acgaccgac gccaaaagg aagaggaag 
      ttcgacggg gacttcgag ttcgcagaa gaggatgag 
      tcgaaagcg gccaagtca ggcgagaag aaacggtgg 
      atacacgat ccctccagt gatctctgc aggccatta 
      aattgcaaa aagaaggaa atctgcttg ttggaagat 
      tcatttacc gcggtgtgc gtatcgaga aaagaactc 
      aaaaagaac ggtgacatc gttgtgcct aaatcgacc 
      gtaaaaact gaagagtcg aagaatgaa gacgatgac 
      gatgatgat gtgttttac gacacggag gacgacccc 
      gatgacacc gacgaggat ttaaacgac tcagtagtg 
      tgtaagccg tgtccggtg gtcaaaccg acgttcttg 
      tgcggttct gataacagg acttacagt tccttgtgt 
      agattagat taccacaac tgcatccac cacacgtcg 
      atcagagtg acgtgcaag ggattctgt ccttgtaaa 
      gaaaatgag gcgcatctt cggaaaaag cagcgacaa 
      tcggaacga ttgaattcg ttcatgaat aagtataaa 
      gccactttg gataaaagt ggaactgtt ggtggatcc 
      attaatggc gcaaagcct gcgcaaccg tcttctaaa 
      tccgatgta tatacattc accccacaa gacttcaaa 
      tacgaaaac aaacattac aaatacata aaatataca 
      aagtacaac aaggacaat aacaatatt ttatacgcg 
      gaggataag gaccggctt cgtggctat aatgaagtt 
      accgacaac aaactttac agtaactca attggggta 
      cttgaccct gcgatttct ccctcgaaa cagtgttct 
      ccggccgca ctccaagca atgggtaat cgcttactc 
      gactggttt tctgttgtt atggcagat tcgtcaaaa 
      cggcgaagg cctcggaac aagtcgaag gctcacttt 
      ccatccgcg tgcaagggc gaagtgaga tggatgttc 
      caacacttg gacgccaat tccgacgct aaactctcc 
      ttgcaagaa ctctacgac ttagagcac gaccagagc 
      gaagtgtgt ttgaaaccc ttcctgcaa caatgcgac 
      atcgatcga gacgtcata gtcacacca ctagaatgg 
      tgtcgatgt tttcaacga acggaacgg ccgtgtgcc 
      gccgtcaag cgcaaaata acacccgaa cttttaggg 
      gtgtacgtg cccgattgc gacaaccag ggttactat 
      cgtccaacg cagtgccac agtgccatc ggcatgtgc 
      tggtgcgtg gacaaacac ggcgtggag ttcgccaac 
      acccgcacc cacgcgaaa cctaattgc gaggcccta 
      ataaataaa tcaaacgga gtggcgaag cagacgagc 
      cctgaagac gccagtgac gacgaggat ggtgacgat 
      gttgagcag gacatcgaa gggagcgca gaccatcct 
      ggagactac taa
    mRNA sequence
    • >TC034543 RA
      aatcaaaaa gtggtcgtg gtttgacgc ggacggtcg 
      tcagtgagg tagagtacg tttgagcgg ccaagtgca 
      gcgaaagtg aaccaaatc aaataaaaa aggacgacg 
      tgttgtccc attatcctt aaaatcggc gaggacgcg 
      ccccaatgc gagtgcttc tggtcctgg tcgtcgcgg 
      cggccgtcg tgtgcacga ccgacgcca aaaggaaga 
      ggaagttcg acggggact tcgagttcg cagaagagg 
      atgagtcga aagcggcca agtcaggcg agaagaaac 
      ggtggatac acgatccct ccagtgatc tctgcaggc 
      cattaaatt gcaaaaaga aggaaatct gcttgttgg 
      aagattcat ttaccgcgg tgtgcgtat cgagaaaag 
      aactcaaaa agaacggtg acatcgttg tgcctaaat 
      cgaccgtaa aaactgaag agtcgaaga atgaagacg 
      atgacgatg atgatgtgt tttacgaca cggaggacg 
      accccgatg acaccgacg aggatttaa acgactcag 
      tagtgtgta agccgtgtc cggtggtca aaccgacgt 
      tcttgtgcg gttctgata acaggactt acagttcct 
      tgtgtagat tagattacc acaactgca tccaccaca 
      cgtcgatca gagtgacgt gcaagggat tctgtcctt 
      gtaaagaaa atgaggcgc atcttcgga aaaagcagc 
      gacaatcgg aacgattga attcgttca tgaataagt 
      ataaagcca ctttggata aaagtggaa ctgttggtg 
      gatccatta atggcgcaa agcctgcgc aaccgtctt 
      ctaaatccg atgtatata cattcaccc cacaagact 
      tcaaatacg aaaacaaac attacaaat acataaaat 
      atacaaagt acaacaagg acaataaca atattttat 
      acgcggagg ataaggacc ggcttcgtg gctataatg 
      aagttaccg acaacaaac tttacagta actcaattg 
      gggtacttg accctgcga tttctccct cgaaacagt 
      gttctccgg ccgcactcc aagcaatgg gtaatcgct 
      tactcgact ggttttctg ttgttatgg cagattcgt 
      caaaacggc gaaggcctc ggaacaagt cgaaggctc 
      actttccat ccgcgtgca agggcgaag tgagatgga 
      tgttccaac acttggacg ccaattccg acgctaaac 
      tctccttgc aagaactct acgacttag agcacgacc 
      agagcgaag tgtgtttga aacccttcc tgcaacaat 
      gcgacatcg atcgagacg tcatagtca caccactag 
      aatggtgtc gatgttttc aacgaacgg aacggccgt 
      gtgccgccg tcaagcgca aaataacac ccgaacttt 
      taggggtgt acgtgcccg attgcgaca accagggtt 
      actatcgtc caacgcagt gccacagtg ccatcggca 
      tgtgctggt gcgtggaca aacacggcg tggagttcg 
      ccaacaccc gcacccacg cgaaaccta attgcgagg 
      ccctaataa ataaatcaa acggagtgg cgaagcaga 
      cgagccctg aagacgcca gtgacgacg aggatggtg 
      acgatgttg agcaggaca tcgaaggga gcgcagacc 
      atcctggag actactaat tagcgccat ctacactat 
      tattgcctc ttactttga cttttttta tgtggcttt 
      tgtattttt tataatgtt aaacaaaga acttcctat 
      aagagtcgg tgtataata taatgaatg attttgatt 
      tttatatag gataccttg tcgcgggta aagtgtagt 
      aggcaatat tgtattgtt gcatattaa gactaatat 
      tttcttaca cattccaca gttgccatt gctttcgtt 
      aaggactac acccttttt catattaat gtgcatcga 
      agtgggcct cctgaaaaa tgaaaaccg gccattaaa 
      ttaaaggtg catttggac ttccgtaac attactatg 
      aagaagggt gtaacaatt ggttaaaaa gtgacagta 
      cttttattt ctaagttta gttaagttt cttaaagtt 
      tattgttgt aaataacta agtgattta ttttaaata 
      ctctctcac cagttacgt tacttaatc attgttcag 
      gtcagcatg tgtgtacgt tattttact gtgtttccg 
      ctattatct ttaggtaga aatagtgat ctgatattc 
      cacagcttc atgtttgtg ccgtctgaa gccatacat 
      aatacgcat ttatgtatt agataatta ctattgtaa 
      taattgtta aatttagaa attatttat gtgattttg 
      atgtccact ttaattaat tactactct agattaata 
      tatttacga agcattatt tttcataga cttagacaa 
      attatattt agagtaatt cctttgtat gtaaacgaa 
      tggtactat gtacataat gaaggtact attacgaac 
      tgacaaccc gatttttac tatcatttt gcagaagtt 
      aatgccaat cttgtttaa aactttggc aatttattt 
      attacttta aaagtctag tgtacaaaa acatgtctt 
      gagtttaac tgacggtgc acctttgta catcaaaca 
      tgtttttgt tgtatgttt tcaacatat atttaataa 
      aactagagc tcatac