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Gene information
General information
TC004556 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid6: 476526..484214
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004556
    iBeetle screen iB_00721
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004556 RA
      MMRRDGEDS NDNKPEHEQ YRKLFIGGL DYRTTDESL 
      KKHFEKWGE IVDVVVMKD PTTKRSRGF GFITYSRAH 
      MVDDAQNDR PHKVDGRVV EPKRAVPRQ DIGRPEAGA 
      TVKKLFVGG LKDDVEEED LRECFKSYG TILSCTIVT 
      DKDTGKKRG FGFIEFEDY DPVDKICLY RHHQIHGKH 
      IDVKKALSK AEMDRANQG GGNMGGGGG PRGGRGGGR 
      GNYSGGGGG GNWNRGGPS GDWNNSQGG YNSGNSWGN 
      NPWDNQSGN NWGGAQSSG YNSGANWGG SNDNYSGGY 
      QQNYGGGPV RNNYQQNRP APYNNPGSG YGGNYGNQN 
      GGNLGGPGN RRYNNM
    CDS sequence
    • >TC004556 RA
      atgatgcgt cgcgacggt gaagactcc aatgacaac 
      aaaccggaa catgaacag taccgcaaa ttattcatc 
      ggcggtttg gattatcgg acaaccgat gaatcgctg 
      aagaaacac tttgaaaaa tggggcgaa atcgtcgat 
      gttgtcgta atgaaggac ccaacaaca aagcggtcg 
      cgcgggttt ggcttcatt acgtattcc agggcacac 
      atggttgac gacgcccag aacgatcgc ccccataaa 
      gtcgatggg cgggtggtc gagcccaag cgagccgtc 
      ccaagacag gacataggg aggcccgaa gcgggggcc 
      actgtcaaa aaactattc gttgggggg ttaaaggac 
      gacgtggag gaggaggat ctaagggag tgtttcaaa 
      tcttatggt accatcctg tcttgtacc atcgtcact 
      gataaagac accggcaag aagcgcgga tttggattt 
      attgaattc gaggattat gaccctgtt gataaaatt 
      tgtttgtac cgccaccac caaatccac ggcaagcac 
      atagacgtg aagaaggct ttgagcaaa gcagagatg 
      gaccgtgcg aaccaagga ggcggcaat atgggcggt 
      gggggcggt ccgcgtgga ggccgtggc ggcggccgg 
      ggcaattac agcggcggc ggcggcggc ggtaactgg 
      aatcgtggg ggccctagc ggcgattgg aacaactcg 
      caagggggc tacaacagc gggaattcg tggggcaac 
      aacccttgg gataaccaa tctggtaac aattggggc 
      ggtgctcaa agttcgggg tacaattct ggggcgaat 
      tggggcggt tccaatgat aactacagc gggggttat 
      cagcagaac tacggaggg ggccctgtg cgcaacaac 
      taccaacag aacagacca gcaccttac aataatcct 
      ggcagcggt tatggcggc aattatggc aatcaaaac 
      ggtggaaat ctaggtggt ccaggcaac aggagatac 
      aataacatg taa
    mRNA sequence
    • >TC004556 RA
      cggttccca ggtgggcat tgttgtgtt ggatcaagt 
      agttttagg aaattttgc aagttgtcc taacttttt 
      tatttattt taacttcaa ctcaatttt cccaatctg 
      ttttgtgaa tttggaagg tgtttccta tgatgcgtc 
      gcgacggtg aagactcca atgacaaca aaccggaac 
      atgaacagt accgcaaat tattcatcg gcggtttgg 
      attatcgga caaccgatg aatcgctga agaaacact 
      ttgaaaaat ggggcgaaa tcgtcgatg ttgtcgtaa 
      tgaaggacc caacaacaa agcggtcgc gcgggtttg 
      gcttcatta cgtattcca gggcacaca tggttgacg 
      acgcccaga acgatcgcc cccataaag tcgatgggc 
      gggtggtcg agcccaagc gagccgtcc caagacagg 
      acataggga ggcccgaag cgggggcca ctgtcaaaa 
      aactattcg ttggggggt taaaggacg acgtggagg 
      aggaggatc taagggagt gtttcaaat cttatggta 
      ccatcctgt cttgtacca tcgtcactg ataaagaca 
      ccggcaaga agcgcggat ttggattta ttgaattcg 
      aggattatg accctgttg ataaaattt gtttgtacc 
      gccaccacc aaatccacg gcaagcaca tagacgtga 
      agaaggctt tgagcaaag cagagatgg accgtgcga 
      accaaggag gcggcaata tgggcggtg ggggcggtc 
      cgcgtggag gccgtggcg gcggccggg gcaattaca 
      gcggcggcg gcggcggcg gtaactgga atcgtgggg 
      gccctagcg gcgattgga acaactcgc aagggggct 
      acaacagcg ggaattcgt ggggcaaca acccttggg 
      ataaccaat ctggtaaca attggggcg gtgctcaaa 
      gttcggggt acaattctg gggcgaatt ggggcggtt 
      ccaatgata actacagcg ggggttatc agcagaact 
      acggagggg gccctgtgc gcaacaact accaacaga 
      acagaccag caccttaca ataatcctg gcagcggtt 
      atggcggca attatggca atcaaaacg gtggaaatc 
      taggtggtc caggcaaca ggagataca ataacatgt 
      aagtgcaac tgggcaacg ctgctaaaa cagtgccag 
      gtgtacata aatttcata tccgtatcc tcaaaaaaa 
      catataata tttaattat ttatgaaat gtagttcac 
      caccggaat attaacacg tttctgtga gaatataaa 
      ttttagttt gccacgtta cagacattt cgaggtgtt 
      gttagtgat tctttgtgt attaattgt attattggt 
      cattggatc ttacagcgc tacggcgcg cctatctta 
      ggttactat tactagata taggttact tgtgtactt 
      tattattca ttaaagttc atgaaatta ttacaagta 
      tttttcata ttttgcgtt gagaggata ggttagagc 
      taacctatg tatcaagat ggacaatgg atttgtata 
      cagagcagg tcttgactg taggaagaa cagtttagt 
      gtagaatga ctgtgtaag caagcaggt tatgtctag 
      gtatgtata gttttagat acgttttag gatgtaaac 
      tctcactat ttagctcag gtttctttt aatgcgagt 
      caggttaca tatgctagt gtatcgtta caaagctaa 
      cctgggtta ggtagacac tgattttgt catctcatt 
      cacatcaaa ctcttattt gttccgcca tttcaattt 
      attttcaat ttttaacac gcacgtacg gacacctat 
      aattaggat acgacacga cgtacaaat aaaaagtaa 
      aaatgaat