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Gene information
General information
TC004551 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2671744..2675986
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004551
    iBeetle screen iB_00720

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004551 RA
      MLRLETNLG WSDVHVKKG TPSHGQLIA SHPDEEALK 
      QGRDALVAT KATIHLMNM FCHKHHLSP ADCSKYISS 
      FPLASTSLE SSCQKQHKL CSQEELLSP YRSFDGTCN 
      NEHDGPRGA AYTAYRRLL HAEYLDGVQ EPRRAVNKK 
      LLPSARLVS STLIKGNDE SHSDLTQAV AQWSEFIEH 
      DLSHTATSK MVHSDSTIE CCASSGNHL SPRYIHPFC 
      APISVPSDD RYYAQHGLD CMTYVRSVP AFRHDCTFG 
      PLEQVNQAT HFLDFSQIY GTTLKKAAI LRTYDEGQL 
      DFTTRHDKV FLPVSHSAG DDCQLSEDN SLCFVSGDS 
      RVNIHPQLT AMHTIWLRE HNRVAKVLS ELNPAWDDE 
      TLFQEARKI VTAEMQHIT YNEWLPLVL GKKYFTKIQ 
      KYNSYDENV NPSVSNEFA TAAVRVLNS LKDGKLNLY 
      KEDRLVNTS LNLRNHFNN PDLVEEPGY LDALIRGLA 
      TQSSQQLDL KFPDDISTH LFSNGAFGF DIFSLDIQR 
      GRDHGLPPY TSYRTLCGL PEVSQFKDL SDVMSPEVI 
      ESLSRVYNS PRDIDLIAG GIAEKPAGD SLFGPTFSC 
      IVADQFLRT RRGDRYFYT NENQPAPFG NAQLREIEK 
      VTLARIFCD NGDDIEMMQ PQVFKRISE SNRLMKCDS 
      GDIPRVNLR MWMDSRRKY Y
    • >TC004551 RB
      MMSRKLAMP LLGVLLWVI SIHAGTFAT ATLLPPPLT 
      GFGSTTKTI IQNHNHDGP KITEKDLNR SVSFGNSVI 
      NRMLRLETN LGWSDVHVK KGTPSHGQL IASHPDEEA 
      LKQGRDALV ATKATIHLM NMFCHKHHL SPADCSKYI 
      SSFPLASTS LESSCQKQH KLCSQEELL SPYRSFDGT 
      CNNEHDGPR GAAYTAYRR LLHAEYLDG VQEPRRAVN 
      KKLLPSARL VSSTLIKGN DESHSDLTQ AVAQWSEFI 
      EHDLSHTAT SKMVHSDST IECCASSGN HLSPRYIHP 
      FCAPISVPS DDRYYAQHG LDCMTYVRS VPAFRHDCT 
      FGPLEQVNQ ATHFLDFSQ IYGTTLKKA AILRTYDEG 
      QLDFTTRHD KVFLPVSHS AGDDCQLSE DNSLCFVSG 
      DSRVNIHPQ LTAMHTIWL REHNRVAKV LSELNPAWD 
      DETLFQEAR KIVTAEMQH ITYNEWLPL VLGKKYFTK 
      IQKYNSYDE NVNPSVSNE FATAAVRVL NSLKDGKLN 
      LYKEDRLVN TSLNLRNHF NNPDLVEEP GYLDALIRG 
      LATQSSQQL DLKFPDDIS THLFSNGAF GFDIFSLDI 
      QRGRDHGLP PYTSYRTLC GLPEVSQFK DLSDVMSPE 
      VIESLSRVY NSPRDIDLI AGGIAEKPA GDSLFGPTF 
      SCIVADQFL RTRRGDRYF YTNENQPAP FGNAQLREI 
      EKVTLARIF CDNGDDIEM MQPQVFKRI SESNRLMKC 
      DSGDIPRVN LRMWMDSRR KYY
    CDS sequence
    • >TC004551 RA
      atgctcaga ctcgaaaca aacctggga tggtccgac 
      gtacatgtc aagaaaggt acgccctct cacggacaa 
      ctcatcgca tcgcatcca gacgaagag gccttaaag 
      caaggccgt gacgctctg gttgccacc aaggccacc 
      atccacctc atgaacatg ttctgtcac aagcaccac 
      ttgagtcct gccgactgc tccaagtac ataagttcg 
      ttccctctg gcctccacg tcgttggaa tcctcctgc 
      caaaagcaa cacaaattg tgttcccag gaggagctc 
      ctctctccg taccgttca tttgatggt acttgcaat 
      aacgaacat gatggcccc cgaggggct gcgtacaca 
      gcctatcga cgtctcctc catgctgag tacttagac 
      ggggttcaa gaacctcga cgtgccgtt aacaaaaaa 
      ctattacct tcggctcgc cttgttagt agtactttg 
      atcaaaggg aatgatgaa agccactcc gatttgact 
      caggccgtg gcgcaatgg agcgaattt atcgaacac 
      gatttaagt catacggcc acgagtaaa atggttcac 
      agtgatagt accatcgaa tgttgcgca tcaagtggc 
      aatcattta tctccacgg tacatccac ccgttttgt 
      gcccccatt tcggtacca tccgatgat cggtactac 
      gcccagcat gggttggat tgcatgact tatgttcgt 
      tcggtacca gcgttccgt catgactgt acttttggt 
      cccttggaa caagtcaac caagccacc cattttttg 
      gatttctct cagatttat ggtaccacc ttgaaaaag 
      gcggccatt ttacgtact tatgatgag ggtcaactc 
      gattttaca actcgacat gacaaagtt ttccttccg 
      gtgtcacac agtgcaggc gatgattgt caattgagt 
      gaagataat tccctttgt tttgtatct ggggattcg 
      agggttaat atccaccct cagttaact gccatgcat 
      acaatatgg ctcagagaa cacaacaga gttgccaaa 
      gtcttgtct gagcttaat ccagcttgg gacgatgaa 
      actctgttc caagaagca aggaaaatt gtgacggcg 
      gaaatgcaa catatcacg tacaatgaa tggttgccg 
      ctcgttttg ggcaagaag tacttcacc aaaatacaa 
      aagtacaac agctatgat gaaaacgtt aatccgtca 
      gtgagtaac gagtttgca acggctgcg gtgagagtt 
      ttgaattca ctcaaggac ggaaagttg aatttgtac 
      aaagaggat cgtctagtc aacacttca ctcaactta 
      cgcaaccac ttcaacaat cccgatctt gttgaagaa 
      cctggctac ttggacgcc ttgattcga ggcttggcc 
      actcagtct agtcaacag ctagatctc aaatttccc 
      gatgatata tccacccac ttattctca aacggcgcc 
      ttcggcttc gacatattc agtcttgac atccagcgc 
      ggtcgcgac cacggattg cccccttac acctcctac 
      cggacgtta tgcggcctt cctgaagtc tctcagttc 
      aaagacctc tccgatgtc atgtcacct gaagtcatc 
      gaatcccta tcacgtgtc tacaacagt ccacgtgac 
      attgattta atagccggt ggaattgct gaaaaacca 
      gccggggat tcccttttc ggtccgaca ttttcatgc 
      attgttgct gaccagttt ttgcgaacg aggcgtggc 
      gatcgctac ttttacacc aatgagaat cagccggcg 
      ccctttgga aacgctcag ttgcgggag attgaaaaa 
      gtcactctt gccaggatt ttttgcgat aatggggac 
      gatatcgag atgatgcaa cctcaggtg tttaaacgg 
      attagcgaa agtaataga ctgatgaaa tgtgattcc 
      ggcgacatt ccaagggtc aacttgagg atgtggatg 
      gatagcaga agaaaatat tattag
    • >TC004551 RB
      atgatgagt cgcaagcta gccatgcct ctgctgggg 
      gtgctgctc tgggtgatc tccatccac gcaggtacc 
      ttcgctacg gctacgcta ctaccccct cccttaaca 
      ggcttcgga tcgacgacc aaaaccatc atccaaaat 
      cacaatcac gatggcccg aaaatcaca gaaaaagac 
      ctcaatcgg tcggtctcg ttcggcaac agtgttatc 
      aaccgaatg ctcagactc gaaacaaac ctgggatgg 
      tccgacgta catgtcaag aaaggtacg ccctctcac 
      ggacaactc atcgcatcg catccagac gaagaggcc 
      ttaaagcaa ggccgtgac gctctggtt gccaccaag 
      gccaccatc cacctcatg aacatgttc tgtcacaag 
      caccacttg agtcctgcc gactgctcc aagtacata 
      agttcgttc cctctggcc tccacgtcg ttggaatcc 
      tcctgccaa aagcaacac aaattgtgt tcccaggag 
      gagctcctc tctccgtac cgttcattt gatggtact 
      tgcaataac gaacatgat ggcccccga ggggctgcg 
      tacacagcc tatcgacgt ctcctccat gctgagtac 
      ttagacggg gttcaagaa cctcgacgt gccgttaac 
      aaaaaacta ttaccttcg gctcgcctt gttagtagt 
      actttgatc aaagggaat gatgaaagc cactccgat 
      ttgactcag gccgtggcg caatggagc gaatttatc 
      gaacacgat ttaagtcat acggccacg agtaaaatg 
      gttcacagt gatagtacc atcgaatgt tgcgcatca 
      agtggcaat catttatct ccacggtac atccacccg 
      ttttgtgcc cccatttcg gtaccatcc gatgatcgg 
      tactacgcc cagcatggg ttggattgc atgacttat 
      gttcgttcg gtaccagcg ttccgtcat gactgtact 
      tttggtccc ttggaacaa gtcaaccaa gccacccat 
      tttttggat ttctctcag atttatggt accaccttg 
      aaaaaggcg gccatttta cgtacttat gatgagggt 
      caactcgat tttacaact cgacatgac aaagttttc 
      cttccggtg tcacacagt gcaggcgat gattgtcaa 
      ttgagtgaa gataattcc ctttgtttt gtatctggg 
      gattcgagg gttaatatc caccctcag ttaactgcc 
      atgcataca atatggctc agagaacac aacagagtt 
      gccaaagtc ttgtctgag cttaatcca gcttgggac 
      gatgaaact ctgttccaa gaagcaagg aaaattgtg 
      acggcggaa atgcaacat atcacgtac aatgaatgg 
      ttgccgctc gttttgggc aagaagtac ttcaccaaa 
      atacaaaag tacaacagc tatgatgaa aacgttaat 
      ccgtcagtg agtaacgag tttgcaacg gctgcggtg 
      agagttttg aattcactc aaggacgga aagttgaat 
      ttgtacaaa gaggatcgt ctagtcaac acttcactc 
      aacttacgc aaccacttc aacaatccc gatcttgtt 
      gaagaacct ggctacttg gacgccttg attcgaggc 
      ttggccact cagtctagt caacagcta gatctcaaa 
      tttcccgat gatatatcc acccactta ttctcaaac 
      ggcgccttc ggcttcgac atattcagt cttgacatc 
      cagcgcggt cgcgaccac ggattgccc ccttacacc 
      tcctaccgg acgttatgc ggccttcct gaagtctct 
      cagttcaaa gacctctcc gatgtcatg tcacctgaa 
      gtcatcgaa tccctatca cgtgtctac aacagtcca 
      cgtgacatt gatttaata gccggtgga attgctgaa 
      aaaccagcc ggggattcc cttttcggt ccgacattt 
      tcatgcatt gttgctgac cagtttttg cgaacgagg 
      cgtggcgat cgctacttt tacaccaat gagaatcag 
      ccggcgccc tttggaaac gctcagttg cgggagatt 
      gaaaaagtc actcttgcc aggattttt tgcgataat 
      ggggacgat atcgagatg atgcaacct caggtgttt 
      aaacggatt agcgaaagt aatagactg atgaaatgt 
      gattccggc gacattcca agggtcaac ttgaggatg 
      tggatggat agcagaaga aaatattat tag
    mRNA sequence
    • >TC004551 RA
      ttcgttttt tatattcaa tccaattat tttttattt 
      tcagtttaa acacaaaac aattgcatg aagatgatg 
      agtcgcaag ctagccatg cctctgctg ggggtgctg 
      ctctgggtg atctccatc cacgcaggt accttcgct 
      acggctacg ctactaccc cctccctta acaggtaca 
      agccgcact tataccact tttctcata caccccctt 
      accagcgtg ttgtttatg ttttgattt tcgttaaat 
      ttcgatttc aaaaaagtc aaaaaataa acacacacg 
      cagtctgga gagcaaata aaaatcagc tagtaaaag 
      aaagacaaa aagctggta acgtttgat tgttagata 
      attctgtct agtgttaca accgttctc ttttaggtt 
      gtttaaaaa tcacagaac ttttctagc tctgtgctt 
      attaatgga atagttgta tgcaaacaa aggatttaa 
      tcctgtttg tgattttgt aaattgagg taagattgt 
      tacccttgt ttattgttc atttttttg tcaacttgt 
      gcactacaa tagagttgc gaggttgcg acgtgcaca 
      aaaaattca attcaattc taagctttt ttgtgcgac 
      tttattcgc gaatcgttc tctcttatt actgaactg 
      gagccttca ttgaacgct tcatttcac gcaaccaaa 
      taacttgaa aacaagtta tttgcgtcc caacggctc 
      cttgtccgc ttcactttt ccctaaggc gtgttttta 
      taggcttcg gatcgacga ccaaaacca tcatccaaa 
      atcacaatc acgatggcc cgaaaatca cagaaaaag 
      acctcaatc ggtcggtct cgttcggca acagtgtta 
      tcaaccgaa tgctcagac tcgaaacaa acctgggat 
      ggtccgacg tacatgtca agaaaggta cgccctctc 
      acggacaac tcatcgcat cgcatccag acgaagagg 
      ccttaaagc aaggccgtg acgctctgg ttgccacca 
      aggccacca tccacctca tgaacatgt tctgtcaca 
      agcaccact tgagtcctg ccgactgct ccaagtaca 
      taagttcgt tccctctgg cctccacgt cgttggaat 
      cctcctgcc aaaagcaac acaaattgt gttcccagg 
      aggagctcc tctctccgt accgttcat ttgatggta 
      cttgcaata acgaacatg atggccccc gaggggctg 
      cgtacacag cctatcgac gtctcctcc atgctgagt 
      acttagacg gggttcaag aacctcgac gtgccgtta 
      acaaaaaac tattacctt cggctcgcc ttgttagta 
      gtactttga tcaaaggga atgatgaaa gccactccg 
      atttgactc aggccgtgg cgcaatgga gcgaattta 
      tcgaacacg atttaagtc atacggcca cgagtaaaa 
      tggttcaca gtgatagta ccatcgaat gttgcgcat 
      caagtggca atcatttat ctccacggt acatccacc 
      cgttttgtg cccccattt cggtaccat ccgatgatc 
      ggtactacg cccagcatg ggttggatt gcatgactt 
      atgttcgtt cggtaccag cgttccgtc atgactgta 
      cttttggtc ccttggaac aagtcaacc aagccaccc 
      attttttgg atttctctc agatttatg gtaccacct 
      tgaaaaagg cggccattt tacgtactt atgatgagg 
      gtcaactcg attttacaa ctcgacatg acaaagttt 
      tccttccgg tgtcacaca gtgcaggcg atgattgtc 
      aattgagtg aagataatt ccctttgtt ttgtatctg 
      gggattcga gggttaata tccaccctc agttaactg 
      ccatgcata caatatggc tcagagaac acaacagag 
      ttgccaaag tcttgtctg agcttaatc cagcttggg 
      acgatgaaa ctctgttcc aagaagcaa ggaaaattg 
      tgacggcgg aaatgcaac atatcacgt acaatgaat 
      ggttgccgc tcgttttgg gcaagaagt acttcacca 
      aaatacaaa agtacaaca gctatgatg aaaacgtta 
      atccgtcag tgagtaacg agtttgcaa cggctgcgg 
      tgagagttt tgaattcac tcaaggacg gaaagttga 
      atttgtaca aagaggatc gtctagtca acacttcac 
      tcaacttac gcaaccact tcaacaatc ccgatcttg 
      ttgaagaac ctggctact tggacgcct tgattcgag 
      gcttggcca ctcagtcta gtcaacagc tagatctca 
      aatttcccg atgatatat ccacccact tattctcaa 
      acggcgcct tcggcttcg acatattca gtcttgaca 
      tccagcgcg gtcgcgacc acggattgc ccccttaca 
      cctcctacc ggacgttat gcggccttc ctgaagtct 
      ctcagttca aagacctct ccgatgtca tgtcacctg 
      aagtcatcg aatccctat cacgtgtct acaacagtc 
      cacgtgaca ttgatttaa tagccggtg gaattgctg 
      aaaaaccag ccggggatt cccttttcg gtccgacat 
      tttcatgca ttgttgctg accagtttt tgcgaacga 
      ggcgtggcg atcgctact tttacacca atgagaatc 
      agccggcgc cctttggaa acgctcagt tgcgggaga 
      ttgaaaaag tcactcttg ccaggattt tttgcgata 
      atggggacg atatcgaga tgatgcaac ctcaggtgt 
      ttaaacgga ttagcgaaa gtaatagac tgatgaaat 
      gtgattccg gcgacattc caagggtca acttgagga 
      tgtggatgg atagcagaa gaaaatatt attagtgcc 
      ttaagtcgt ttaaggtag catcagttc aagactgat 
      ttttaatgg ctgttttat tagattttt aagtcaaaa 
      ttgaaattc ttaatttac atatcttta gtaacagta 
      tttatttct ttttatact tgtatgtat agcgacaca 
      aattttgta atgctcagt gcataaaat tgtgcaatt 
      ttatttatt gtataaaat aacatgtaa tgtcgtaag 
      atttttaag tcaaactat attagtttg atttagcgt 
      agaggaatt ttatagcga tgtaaaaag taatggtac 
      aatgttttc tacacggtg gaacaacaa aataaaatt 
      attgattgc aa
    • >TC004551 RB
      ttcgttttt tatattcaa tccaattat tttttattt 
      tcagtttaa acacaaaac aattgcatg aagatgatg 
      agtcgcaag ctagccatg cctctgctg ggggtgctg 
      ctctgggtg atctccatc cacgcaggt accttcgct 
      acggctacg ctactaccc cctccctta acaggcttc 
      ggatcgacg accaaaacc atcatccaa aatcacaat 
      cacgatggc ccgaaaatc acagaaaaa gacctcaat 
      cggtcggtc tcgttcggc aacagtgtt atcaaccga 
      atgctcaga ctcgaaaca aacctggga tggtccgac 
      gtacatgtc aagaaaggt acgccctct cacggacaa 
      ctcatcgca tcgcatcca gacgaagag gccttaaag 
      caaggccgt gacgctctg gttgccacc aaggccacc 
      atccacctc atgaacatg ttctgtcac aagcaccac 
      ttgagtcct gccgactgc tccaagtac ataagttcg 
      ttccctctg gcctccacg tcgttggaa tcctcctgc 
      caaaagcaa cacaaattg tgttcccag gaggagctc 
      ctctctccg taccgttca tttgatggt acttgcaat 
      aacgaacat gatggcccc cgaggggct gcgtacaca 
      gcctatcga cgtctcctc catgctgag tacttagac 
      ggggttcaa gaacctcga cgtgccgtt aacaaaaaa 
      ctattacct tcggctcgc cttgttagt agtactttg 
      atcaaaggg aatgatgaa agccactcc gatttgact 
      caggccgtg gcgcaatgg agcgaattt atcgaacac 
      gatttaagt catacggcc acgagtaaa atggttcac 
      agtgatagt accatcgaa tgttgcgca tcaagtggc 
      aatcattta tctccacgg tacatccac ccgttttgt 
      gcccccatt tcggtacca tccgatgat cggtactac 
      gcccagcat gggttggat tgcatgact tatgttcgt 
      tcggtacca gcgttccgt catgactgt acttttggt 
      cccttggaa caagtcaac caagccacc cattttttg 
      gatttctct cagatttat ggtaccacc ttgaaaaag 
      gcggccatt ttacgtact tatgatgag ggtcaactc 
      gattttaca actcgacat gacaaagtt ttccttccg 
      gtgtcacac agtgcaggc gatgattgt caattgagt 
      gaagataat tccctttgt tttgtatct ggggattcg 
      agggttaat atccaccct cagttaact gccatgcat 
      acaatatgg ctcagagaa cacaacaga gttgccaaa 
      gtcttgtct gagcttaat ccagcttgg gacgatgaa 
      actctgttc caagaagca aggaaaatt gtgacggcg 
      gaaatgcaa catatcacg tacaatgaa tggttgccg 
      ctcgttttg ggcaagaag tacttcacc aaaatacaa 
      aagtacaac agctatgat gaaaacgtt aatccgtca 
      gtgagtaac gagtttgca acggctgcg gtgagagtt 
      ttgaattca ctcaaggac ggaaagttg aatttgtac 
      aaagaggat cgtctagtc aacacttca ctcaactta 
      cgcaaccac ttcaacaat cccgatctt gttgaagaa 
      cctggctac ttggacgcc ttgattcga ggcttggcc 
      actcagtct agtcaacag ctagatctc aaatttccc 
      gatgatata tccacccac ttattctca aacggcgcc 
      ttcggcttc gacatattc agtcttgac atccagcgc 
      ggtcgcgac cacggattg cccccttac acctcctac 
      cggacgtta tgcggcctt cctgaagtc tctcagttc 
      aaagacctc tccgatgtc atgtcacct gaagtcatc 
      gaatcccta tcacgtgtc tacaacagt ccacgtgac 
      attgattta atagccggt ggaattgct gaaaaacca 
      gccggggat tcccttttc ggtccgaca ttttcatgc 
      attgttgct gaccagttt ttgcgaacg aggcgtggc 
      gatcgctac ttttacacc aatgagaat cagccggcg 
      ccctttgga aacgctcag ttgcgggag attgaaaaa 
      gtcactctt gccaggatt ttttgcgat aatggggac 
      gatatcgag atgatgcaa cctcaggtg tttaaacgg 
      attagcgaa agtaataga ctgatgaaa tgtgattcc 
      ggcgacatt ccaagggtc aacttgagg atgtggatg 
      gatagcaga agaaaatat tattagtgc cttaagtcg 
      tttaaggta gcatcagtt caagactga tttttaatg 
      gctgtttta ttagatttt taagtcaaa attgaaatt 
      cttaattta catatcttt agtaacagt atttatttc 
      tttttatac ttgtatgta tagcgacac aaattttgt 
      aatgctcag tgcataaaa ttgtgcaat tttatttat 
      tgtataaaa taacatgta atgtcgtaa gatttttaa 
      gtcaaacta tattagttt gatttagcg tagaggaat 
      tttatagcg atgtaaaaa gtaatggta caatgtttt 
      ctacacggt ggaacaaca aaataaaat tattgattg 
      caa
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