| General information | |||
| TC004551 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 2671744..2675986 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004551 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00720 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC004551 RA
MLRLETNLG WSDVHVKKG TPSHGQLIA SHPDEEALK QGRDALVAT KATIHLMNM FCHKHHLSP ADCSKYISS FPLASTSLE SSCQKQHKL CSQEELLSP YRSFDGTCN NEHDGPRGA AYTAYRRLL HAEYLDGVQ EPRRAVNKK LLPSARLVS STLIKGNDE SHSDLTQAV AQWSEFIEH DLSHTATSK MVHSDSTIE CCASSGNHL SPRYIHPFC APISVPSDD RYYAQHGLD CMTYVRSVP AFRHDCTFG PLEQVNQAT HFLDFSQIY GTTLKKAAI LRTYDEGQL DFTTRHDKV FLPVSHSAG DDCQLSEDN SLCFVSGDS RVNIHPQLT AMHTIWLRE HNRVAKVLS ELNPAWDDE TLFQEARKI VTAEMQHIT YNEWLPLVL GKKYFTKIQ KYNSYDENV NPSVSNEFA TAAVRVLNS LKDGKLNLY KEDRLVNTS LNLRNHFNN PDLVEEPGY LDALIRGLA TQSSQQLDL KFPDDISTH LFSNGAFGF DIFSLDIQR GRDHGLPPY TSYRTLCGL PEVSQFKDL SDVMSPEVI ESLSRVYNS PRDIDLIAG GIAEKPAGD SLFGPTFSC IVADQFLRT RRGDRYFYT NENQPAPFG NAQLREIEK VTLARIFCD NGDDIEMMQ PQVFKRISE SNRLMKCDS GDIPRVNLR MWMDSRRKY Y
- >TC004551 RB
MMSRKLAMP LLGVLLWVI SIHAGTFAT ATLLPPPLT GFGSTTKTI IQNHNHDGP KITEKDLNR SVSFGNSVI NRMLRLETN LGWSDVHVK KGTPSHGQL IASHPDEEA LKQGRDALV ATKATIHLM NMFCHKHHL SPADCSKYI SSFPLASTS LESSCQKQH KLCSQEELL SPYRSFDGT CNNEHDGPR GAAYTAYRR LLHAEYLDG VQEPRRAVN KKLLPSARL VSSTLIKGN DESHSDLTQ AVAQWSEFI EHDLSHTAT SKMVHSDST IECCASSGN HLSPRYIHP FCAPISVPS DDRYYAQHG LDCMTYVRS VPAFRHDCT FGPLEQVNQ ATHFLDFSQ IYGTTLKKA AILRTYDEG QLDFTTRHD KVFLPVSHS AGDDCQLSE DNSLCFVSG DSRVNIHPQ LTAMHTIWL REHNRVAKV LSELNPAWD DETLFQEAR KIVTAEMQH ITYNEWLPL VLGKKYFTK IQKYNSYDE NVNPSVSNE FATAAVRVL NSLKDGKLN LYKEDRLVN TSLNLRNHF NNPDLVEEP GYLDALIRG LATQSSQQL DLKFPDDIS THLFSNGAF GFDIFSLDI QRGRDHGLP PYTSYRTLC GLPEVSQFK DLSDVMSPE VIESLSRVY NSPRDIDLI AGGIAEKPA GDSLFGPTF SCIVADQFL RTRRGDRYF YTNENQPAP FGNAQLREI EKVTLARIF CDNGDDIEM MQPQVFKRI SESNRLMKC DSGDIPRVN LRMWMDSRR KYY
- >TC004551 RA
atgctcaga ctcgaaaca aacctggga tggtccgac gtacatgtc aagaaaggt acgccctct cacggacaa ctcatcgca tcgcatcca gacgaagag gccttaaag caaggccgt gacgctctg gttgccacc aaggccacc atccacctc atgaacatg ttctgtcac aagcaccac ttgagtcct gccgactgc tccaagtac ataagttcg ttccctctg gcctccacg tcgttggaa tcctcctgc caaaagcaa cacaaattg tgttcccag gaggagctc ctctctccg taccgttca tttgatggt acttgcaat aacgaacat gatggcccc cgaggggct gcgtacaca gcctatcga cgtctcctc catgctgag tacttagac ggggttcaa gaacctcga cgtgccgtt aacaaaaaa ctattacct tcggctcgc cttgttagt agtactttg atcaaaggg aatgatgaa agccactcc gatttgact caggccgtg gcgcaatgg agcgaattt atcgaacac gatttaagt catacggcc acgagtaaa atggttcac agtgatagt accatcgaa tgttgcgca tcaagtggc aatcattta tctccacgg tacatccac ccgttttgt gcccccatt tcggtacca tccgatgat cggtactac gcccagcat gggttggat tgcatgact tatgttcgt tcggtacca gcgttccgt catgactgt acttttggt cccttggaa caagtcaac caagccacc cattttttg gatttctct cagatttat ggtaccacc ttgaaaaag gcggccatt ttacgtact tatgatgag ggtcaactc gattttaca actcgacat gacaaagtt ttccttccg gtgtcacac agtgcaggc gatgattgt caattgagt gaagataat tccctttgt tttgtatct ggggattcg agggttaat atccaccct cagttaact gccatgcat acaatatgg ctcagagaa cacaacaga gttgccaaa gtcttgtct gagcttaat ccagcttgg gacgatgaa actctgttc caagaagca aggaaaatt gtgacggcg gaaatgcaa catatcacg tacaatgaa tggttgccg ctcgttttg ggcaagaag tacttcacc aaaatacaa aagtacaac agctatgat gaaaacgtt aatccgtca gtgagtaac gagtttgca acggctgcg gtgagagtt ttgaattca ctcaaggac ggaaagttg aatttgtac aaagaggat cgtctagtc aacacttca ctcaactta cgcaaccac ttcaacaat cccgatctt gttgaagaa cctggctac ttggacgcc ttgattcga ggcttggcc actcagtct agtcaacag ctagatctc aaatttccc gatgatata tccacccac ttattctca aacggcgcc ttcggcttc gacatattc agtcttgac atccagcgc ggtcgcgac cacggattg cccccttac acctcctac cggacgtta tgcggcctt cctgaagtc tctcagttc aaagacctc tccgatgtc atgtcacct gaagtcatc gaatcccta tcacgtgtc tacaacagt ccacgtgac attgattta atagccggt ggaattgct gaaaaacca gccggggat tcccttttc ggtccgaca ttttcatgc attgttgct gaccagttt ttgcgaacg aggcgtggc gatcgctac ttttacacc aatgagaat cagccggcg ccctttgga aacgctcag ttgcgggag attgaaaaa gtcactctt gccaggatt ttttgcgat aatggggac gatatcgag atgatgcaa cctcaggtg tttaaacgg attagcgaa agtaataga ctgatgaaa tgtgattcc ggcgacatt ccaagggtc aacttgagg atgtggatg gatagcaga agaaaatat tattag
- >TC004551 RB
atgatgagt cgcaagcta gccatgcct ctgctgggg gtgctgctc tgggtgatc tccatccac gcaggtacc ttcgctacg gctacgcta ctaccccct cccttaaca ggcttcgga tcgacgacc aaaaccatc atccaaaat cacaatcac gatggcccg aaaatcaca gaaaaagac ctcaatcgg tcggtctcg ttcggcaac agtgttatc aaccgaatg ctcagactc gaaacaaac ctgggatgg tccgacgta catgtcaag aaaggtacg ccctctcac ggacaactc atcgcatcg catccagac gaagaggcc ttaaagcaa ggccgtgac gctctggtt gccaccaag gccaccatc cacctcatg aacatgttc tgtcacaag caccacttg agtcctgcc gactgctcc aagtacata agttcgttc cctctggcc tccacgtcg ttggaatcc tcctgccaa aagcaacac aaattgtgt tcccaggag gagctcctc tctccgtac cgttcattt gatggtact tgcaataac gaacatgat ggcccccga ggggctgcg tacacagcc tatcgacgt ctcctccat gctgagtac ttagacggg gttcaagaa cctcgacgt gccgttaac aaaaaacta ttaccttcg gctcgcctt gttagtagt actttgatc aaagggaat gatgaaagc cactccgat ttgactcag gccgtggcg caatggagc gaatttatc gaacacgat ttaagtcat acggccacg agtaaaatg gttcacagt gatagtacc atcgaatgt tgcgcatca agtggcaat catttatct ccacggtac atccacccg ttttgtgcc cccatttcg gtaccatcc gatgatcgg tactacgcc cagcatggg ttggattgc atgacttat gttcgttcg gtaccagcg ttccgtcat gactgtact tttggtccc ttggaacaa gtcaaccaa gccacccat tttttggat ttctctcag atttatggt accaccttg aaaaaggcg gccatttta cgtacttat gatgagggt caactcgat tttacaact cgacatgac aaagttttc cttccggtg tcacacagt gcaggcgat gattgtcaa ttgagtgaa gataattcc ctttgtttt gtatctggg gattcgagg gttaatatc caccctcag ttaactgcc atgcataca atatggctc agagaacac aacagagtt gccaaagtc ttgtctgag cttaatcca gcttgggac gatgaaact ctgttccaa gaagcaagg aaaattgtg acggcggaa atgcaacat atcacgtac aatgaatgg ttgccgctc gttttgggc aagaagtac ttcaccaaa atacaaaag tacaacagc tatgatgaa aacgttaat ccgtcagtg agtaacgag tttgcaacg gctgcggtg agagttttg aattcactc aaggacgga aagttgaat ttgtacaaa gaggatcgt ctagtcaac acttcactc aacttacgc aaccacttc aacaatccc gatcttgtt gaagaacct ggctacttg gacgccttg attcgaggc ttggccact cagtctagt caacagcta gatctcaaa tttcccgat gatatatcc acccactta ttctcaaac ggcgccttc ggcttcgac atattcagt cttgacatc cagcgcggt cgcgaccac ggattgccc ccttacacc tcctaccgg acgttatgc ggccttcct gaagtctct cagttcaaa gacctctcc gatgtcatg tcacctgaa gtcatcgaa tccctatca cgtgtctac aacagtcca cgtgacatt gatttaata gccggtgga attgctgaa aaaccagcc ggggattcc cttttcggt ccgacattt tcatgcatt gttgctgac cagtttttg cgaacgagg cgtggcgat cgctacttt tacaccaat gagaatcag ccggcgccc tttggaaac gctcagttg cgggagatt gaaaaagtc actcttgcc aggattttt tgcgataat ggggacgat atcgagatg atgcaacct caggtgttt aaacggatt agcgaaagt aatagactg atgaaatgt gattccggc gacattcca agggtcaac ttgaggatg tggatggat agcagaaga aaatattat tag
- >TC004551 RA
ttcgttttt tatattcaa tccaattat tttttattt tcagtttaa acacaaaac aattgcatg aagatgatg agtcgcaag ctagccatg cctctgctg ggggtgctg ctctgggtg atctccatc cacgcaggt accttcgct acggctacg ctactaccc cctccctta acaggtaca agccgcact tataccact tttctcata caccccctt accagcgtg ttgtttatg ttttgattt tcgttaaat ttcgatttc aaaaaagtc aaaaaataa acacacacg cagtctgga gagcaaata aaaatcagc tagtaaaag aaagacaaa aagctggta acgtttgat tgttagata attctgtct agtgttaca accgttctc ttttaggtt gtttaaaaa tcacagaac ttttctagc tctgtgctt attaatgga atagttgta tgcaaacaa aggatttaa tcctgtttg tgattttgt aaattgagg taagattgt tacccttgt ttattgttc atttttttg tcaacttgt gcactacaa tagagttgc gaggttgcg acgtgcaca aaaaattca attcaattc taagctttt ttgtgcgac tttattcgc gaatcgttc tctcttatt actgaactg gagccttca ttgaacgct tcatttcac gcaaccaaa taacttgaa aacaagtta tttgcgtcc caacggctc cttgtccgc ttcactttt ccctaaggc gtgttttta taggcttcg gatcgacga ccaaaacca tcatccaaa atcacaatc acgatggcc cgaaaatca cagaaaaag acctcaatc ggtcggtct cgttcggca acagtgtta tcaaccgaa tgctcagac tcgaaacaa acctgggat ggtccgacg tacatgtca agaaaggta cgccctctc acggacaac tcatcgcat cgcatccag acgaagagg ccttaaagc aaggccgtg acgctctgg ttgccacca aggccacca tccacctca tgaacatgt tctgtcaca agcaccact tgagtcctg ccgactgct ccaagtaca taagttcgt tccctctgg cctccacgt cgttggaat cctcctgcc aaaagcaac acaaattgt gttcccagg aggagctcc tctctccgt accgttcat ttgatggta cttgcaata acgaacatg atggccccc gaggggctg cgtacacag cctatcgac gtctcctcc atgctgagt acttagacg gggttcaag aacctcgac gtgccgtta acaaaaaac tattacctt cggctcgcc ttgttagta gtactttga tcaaaggga atgatgaaa gccactccg atttgactc aggccgtgg cgcaatgga gcgaattta tcgaacacg atttaagtc atacggcca cgagtaaaa tggttcaca gtgatagta ccatcgaat gttgcgcat caagtggca atcatttat ctccacggt acatccacc cgttttgtg cccccattt cggtaccat ccgatgatc ggtactacg cccagcatg ggttggatt gcatgactt atgttcgtt cggtaccag cgttccgtc atgactgta cttttggtc ccttggaac aagtcaacc aagccaccc attttttgg atttctctc agatttatg gtaccacct tgaaaaagg cggccattt tacgtactt atgatgagg gtcaactcg attttacaa ctcgacatg acaaagttt tccttccgg tgtcacaca gtgcaggcg atgattgtc aattgagtg aagataatt ccctttgtt ttgtatctg gggattcga gggttaata tccaccctc agttaactg ccatgcata caatatggc tcagagaac acaacagag ttgccaaag tcttgtctg agcttaatc cagcttggg acgatgaaa ctctgttcc aagaagcaa ggaaaattg tgacggcgg aaatgcaac atatcacgt acaatgaat ggttgccgc tcgttttgg gcaagaagt acttcacca aaatacaaa agtacaaca gctatgatg aaaacgtta atccgtcag tgagtaacg agtttgcaa cggctgcgg tgagagttt tgaattcac tcaaggacg gaaagttga atttgtaca aagaggatc gtctagtca acacttcac tcaacttac gcaaccact tcaacaatc ccgatcttg ttgaagaac ctggctact tggacgcct tgattcgag gcttggcca ctcagtcta gtcaacagc tagatctca aatttcccg atgatatat ccacccact tattctcaa acggcgcct tcggcttcg acatattca gtcttgaca tccagcgcg gtcgcgacc acggattgc ccccttaca cctcctacc ggacgttat gcggccttc ctgaagtct ctcagttca aagacctct ccgatgtca tgtcacctg aagtcatcg aatccctat cacgtgtct acaacagtc cacgtgaca ttgatttaa tagccggtg gaattgctg aaaaaccag ccggggatt cccttttcg gtccgacat tttcatgca ttgttgctg accagtttt tgcgaacga ggcgtggcg atcgctact tttacacca atgagaatc agccggcgc cctttggaa acgctcagt tgcgggaga ttgaaaaag tcactcttg ccaggattt tttgcgata atggggacg atatcgaga tgatgcaac ctcaggtgt ttaaacgga ttagcgaaa gtaatagac tgatgaaat gtgattccg gcgacattc caagggtca acttgagga tgtggatgg atagcagaa gaaaatatt attagtgcc ttaagtcgt ttaaggtag catcagttc aagactgat ttttaatgg ctgttttat tagattttt aagtcaaaa ttgaaattc ttaatttac atatcttta gtaacagta tttatttct ttttatact tgtatgtat agcgacaca aattttgta atgctcagt gcataaaat tgtgcaatt ttatttatt gtataaaat aacatgtaa tgtcgtaag atttttaag tcaaactat attagtttg atttagcgt agaggaatt ttatagcga tgtaaaaag taatggtac aatgttttc tacacggtg gaacaacaa aataaaatt attgattgc aa
- >TC004551 RB
ttcgttttt tatattcaa tccaattat tttttattt tcagtttaa acacaaaac aattgcatg aagatgatg agtcgcaag ctagccatg cctctgctg ggggtgctg ctctgggtg atctccatc cacgcaggt accttcgct acggctacg ctactaccc cctccctta acaggcttc ggatcgacg accaaaacc atcatccaa aatcacaat cacgatggc ccgaaaatc acagaaaaa gacctcaat cggtcggtc tcgttcggc aacagtgtt atcaaccga atgctcaga ctcgaaaca aacctggga tggtccgac gtacatgtc aagaaaggt acgccctct cacggacaa ctcatcgca tcgcatcca gacgaagag gccttaaag caaggccgt gacgctctg gttgccacc aaggccacc atccacctc atgaacatg ttctgtcac aagcaccac ttgagtcct gccgactgc tccaagtac ataagttcg ttccctctg gcctccacg tcgttggaa tcctcctgc caaaagcaa cacaaattg tgttcccag gaggagctc ctctctccg taccgttca tttgatggt acttgcaat aacgaacat gatggcccc cgaggggct gcgtacaca gcctatcga cgtctcctc catgctgag tacttagac ggggttcaa gaacctcga cgtgccgtt aacaaaaaa ctattacct tcggctcgc cttgttagt agtactttg atcaaaggg aatgatgaa agccactcc gatttgact caggccgtg gcgcaatgg agcgaattt atcgaacac gatttaagt catacggcc acgagtaaa atggttcac agtgatagt accatcgaa tgttgcgca tcaagtggc aatcattta tctccacgg tacatccac ccgttttgt gcccccatt tcggtacca tccgatgat cggtactac gcccagcat gggttggat tgcatgact tatgttcgt tcggtacca gcgttccgt catgactgt acttttggt cccttggaa caagtcaac caagccacc cattttttg gatttctct cagatttat ggtaccacc ttgaaaaag gcggccatt ttacgtact tatgatgag ggtcaactc gattttaca actcgacat gacaaagtt ttccttccg gtgtcacac agtgcaggc gatgattgt caattgagt gaagataat tccctttgt tttgtatct ggggattcg agggttaat atccaccct cagttaact gccatgcat acaatatgg ctcagagaa cacaacaga gttgccaaa gtcttgtct gagcttaat ccagcttgg gacgatgaa actctgttc caagaagca aggaaaatt gtgacggcg gaaatgcaa catatcacg tacaatgaa tggttgccg ctcgttttg ggcaagaag tacttcacc aaaatacaa aagtacaac agctatgat gaaaacgtt aatccgtca gtgagtaac gagtttgca acggctgcg gtgagagtt ttgaattca ctcaaggac ggaaagttg aatttgtac aaagaggat cgtctagtc aacacttca ctcaactta cgcaaccac ttcaacaat cccgatctt gttgaagaa cctggctac ttggacgcc ttgattcga ggcttggcc actcagtct agtcaacag ctagatctc aaatttccc gatgatata tccacccac ttattctca aacggcgcc ttcggcttc gacatattc agtcttgac atccagcgc ggtcgcgac cacggattg cccccttac acctcctac cggacgtta tgcggcctt cctgaagtc tctcagttc aaagacctc tccgatgtc atgtcacct gaagtcatc gaatcccta tcacgtgtc tacaacagt ccacgtgac attgattta atagccggt ggaattgct gaaaaacca gccggggat tcccttttc ggtccgaca ttttcatgc attgttgct gaccagttt ttgcgaacg aggcgtggc gatcgctac ttttacacc aatgagaat cagccggcg ccctttgga aacgctcag ttgcgggag attgaaaaa gtcactctt gccaggatt ttttgcgat aatggggac gatatcgag atgatgcaa cctcaggtg tttaaacgg attagcgaa agtaataga ctgatgaaa tgtgattcc ggcgacatt ccaagggtc aacttgagg atgtggatg gatagcaga agaaaatat tattagtgc cttaagtcg tttaaggta gcatcagtt caagactga tttttaatg gctgtttta ttagatttt taagtcaaa attgaaatt cttaattta catatcttt agtaacagt atttatttc tttttatac ttgtatgta tagcgacac aaattttgt aatgctcag tgcataaaa ttgtgcaat tttatttat tgtataaaa taacatgta atgtcgtaa gatttttaa gtcaaacta tattagttt gatttagcg tagaggaat tttatagcg atgtaaaaa gtaatggta caatgtttt ctacacggt ggaacaaca aaataaaat tattgattg caa
