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Gene information
General information
TC004545 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2788217..2837159
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004545
    iBeetle screen iB_00718

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004545 RA
      MSDSNSSCS PDLPGNFST IFGQTDVAQ WEALLTIVT 
      LTLIILVTI VGNVLVILS VFTYKPLRI VQNFFIVSL 
      AVADLTVAI LVMPLNVML SILGRWVFD IHICKMWLT 
      SDVLCCTAS ILNLCAIAL DRFWAITDP INYAQKRTL 
      KRVLLMIGG VWVLSLVIS SPPLIGWND WPEADEFHL 
      IQECKLTER QGYVIYSSL GSFFIPLFI MTIVYVEIF 
      IATKRRLRE RAQASKINA ISRNQSALI SNKEAQHDR 
      ESVSSETNH NEQPETNGK PKDKKKKTK KKRKDDNVD 
      NNAYLKPIL VHEDSLTDN PETSSISQR RGSNPCTSD 
      NHKITVSES TTVSNSPQL NKVLLPEAT ATVKKPGVV 
      YQFIEEKQR ISLSKERRA ARTLGIIMG VFVVCWLPF 
      FLMYVTIPF CPSCCPSKR LKNFITWLG YINSVLNPI 
      IYTIFNLDF RRAFRKLLG MKP
    • >TC004545 RB
      MSDSNSSCS PDLPGNFST IFGQTDVAQ WEALLTIVT 
      LTLIILVTI VGNVLVILS VFTYKPLRI VQNFFIVSL 
      AVADLTVAI LVMPLNVML SILGRWVFD IHICKMWLT 
      SDVLCCTAS ILNLCAIAL DRFWAITDP INYAQKRTL 
      KRVLLMIGG VWVLSLVIS SPPLIGWND WPEADEFHL 
      IQECKLTER QGYVIYSSL GSFFIPLFI MTIVYVEIF 
      IATKRRLRE RAQASKINA ISRNQSALI SNKEAQHDR 
      ESVSSETNH NEQPETNGK PKDKKKKTK KKRKDDNVD 
      NNAYLKPIL VHEDSLTDN PETSSISQR RGSNPCTSD 
      NHKITVSES TTVSNSPQL NKVLLPEAT ATVKKPGVV 
      YQFIEEKQR ISLSKERRA ARTLGIIMG VFVVCWLPF 
      FLMYVTIPF CPSCCPSKR LKNFITWLG YINSVLNPI 
      IYTIFNLDF RRAFRKLLG MKP
    CDS sequence
    • >TC004545 RA
      atgtccgac tcaaacagc agctgctcc cccgacctg 
      cccggcaac ttttcgacg attttcggc cagacggac 
      gtcgcccaa tgggaagcc ctcctcaca atcgtcact 
      ctcaccctc atcatcctc gtcacaatc gtcggcaac 
      gtcctcgtc attctcagc gtcttcacc tacaaaccg 
      ctccgaatc gtccaaaac ttcttcatc gtgtcgctc 
      gcagtcgcc gacctcaca gtcgccatc ctcgtcatg 
      cccctgaac gttatgctg tcgatcttg ggacgatgg 
      gtgttcgac atccacatc tgcaaaatg tggctgact 
      tcggacgtc ctctgctgc acggcttca attctgaat 
      ctgtgtgcg attgcgttg gaccgcttt tgggccatc 
      acagacccc attaactac gcgcaaaag cgcaccctg 
      aaaagggtc ctgttgatg attggaggc gtgtgggtt 
      ttgtcacta gtgatcagt tctccgcca ctgatcggc 
      tggaacgac tggcctgaa gccgacgag ttccacttg 
      attcaagag tgcaagtta acggaacgg caaggctat 
      gtcatttac tcatcactg gggtcgttc ttcatccct 
      ttgttcatc atgacgatc gtctacgtt gagattttc 
      atcgccacg aagcgccgc ttgagagag agggcacaa 
      gcctcgaaa atcaacgca atctcgcgg aaccaatcg 
      gctctgatc agcaacaaa gaagcacag catgatcgc 
      gagagtgtc agcagcgaa accaatcat aacgagcag 
      cccgaaacc aacggcaag cccaaagac aagaagaag 
      aaaaccaag aagaagcga aaagacgac aacgtcgac 
      aacaacgcc tatctcaaa ccgattcta gtccatgaa 
      gattctcta actgacaat ccggaaact tcctcaatc 
      tcgcaacgg cggggttcc aacccttgc acttccgac 
      aaccacaaa atcacagtt tccgagtcc acgacggtg 
      tcaaactcg ccccagctg aacaaggtt ttgttgccg 
      gaagccacc gccacggtg aaaaagccg ggagtggtt 
      taccaattc atcgaagag aaacaacgc atttccttg 
      tcgaaggag agacgcgcg gcgcgaact cttggaata 
      ataatggga gtgtttgtg gtgtgttgg ttgccgttc 
      ttcctcatg tatgtaacg atccctttc tgccccagt 
      tgttgcccc tccaagaga ctcaagaac tttatcact 
      tggttaggt tacattaat tccgtgctc aatcccatt 
      atttatacg atttttaat ctcgacttt cggcgcgct 
      tttagaaag cttctaggg atgaagccg tga
    • >TC004545 RB
      atgtccgac tcaaacagc agctgctcc cccgacctg 
      cccggcaac ttttcgacg attttcggc cagacggac 
      gtcgcccaa tgggaagcc ctcctcaca atcgtcact 
      ctcaccctc atcatcctc gtcacaatc gtcggcaac 
      gtcctcgtc attctcagc gtcttcacc tacaaaccg 
      ctccgaatc gtccaaaac ttcttcatc gtgtcgctc 
      gcagtcgcc gacctcaca gtcgccatc ctcgtcatg 
      cccctgaac gttatgctg tcgatcttg ggacgatgg 
      gtgttcgac atccacatc tgcaaaatg tggctgact 
      tcggacgtc ctctgctgc acggcttca attctgaat 
      ctgtgtgcg attgcgttg gaccgcttt tgggccatc 
      acagacccc attaactac gcgcaaaag cgcaccctg 
      aaaagggtc ctgttgatg attggaggc gtgtgggtt 
      ttgtcacta gtgatcagt tctccgcca ctgatcggc 
      tggaacgac tggcctgaa gccgacgag ttccacttg 
      attcaagag tgcaagtta acggaacgg caaggctat 
      gtcatttac tcatcactg gggtcgttc ttcatccct 
      ttgttcatc atgacgatc gtctacgtt gagattttc 
      atcgccacg aagcgccgc ttgagagag agggcacaa 
      gcctcgaaa atcaacgca atctcgcgg aaccaatcg 
      gctctgatc agcaacaaa gaagcacag catgatcgc 
      gagagtgtc agcagcgaa accaatcat aacgagcag 
      cccgaaacc aacggcaag cccaaagac aagaagaag 
      aaaaccaag aagaagcga aaagacgac aacgtcgac 
      aacaacgcc tatctcaaa ccgattcta gtccatgaa 
      gattctcta actgacaat ccggaaact tcctcaatc 
      tcgcaacgg cggggttcc aacccttgc acttccgac 
      aaccacaaa atcacagtt tccgagtcc acgacggtg 
      tcaaactcg ccccagctg aacaaggtt ttgttgccg 
      gaagccacc gccacggtg aaaaagccg ggagtggtt 
      taccaattc atcgaagag aaacaacgc atttccttg 
      tcgaaggag agacgcgcg gcgcgaact cttggaata 
      ataatggga gtgtttgtg gtgtgttgg ttgccgttc 
      ttcctcatg tatgtaacg atccctttc tgccccagt 
      tgttgcccc tccaagaga ctcaagaac tttatcact 
      tggttaggt tacattaat tccgtgctc aatcccatt 
      atttatacg atttttaat ctcgacttt cggcgcgct 
      tttagaaag cttctaggg atgaagccg tga
    mRNA sequence
    • >TC004545 RA
      gtccagatt cgacgcttc ggggttttg agcgagaaa 
      gcgcgcgtt taacgaaac ttattttca acttggtgc 
      gatgtggac gcttatttt tagttgcgt ttgagtacg 
      gacctggaa acgatggac caattagga ttttgccgc 
      agaatccgc agttacgga gcgtaatag agaccggcg 
      agattggat aaattgttg ggagcttcc cgaagatgt 
      aattaagct ttgcgacca tgtccgact caaacagca 
      gctgctccc ccgacctgc ccggcaact tttcgacga 
      ttttcggcc agacggacg tcgcccaat gggaagccc 
      tcctcacaa tcgtcactc tcaccctca tcatcctcg 
      tcacaatcg tcggcaacg tcctcgtca ttctcagcg 
      tcttcacct acaaaccgc tccgaatcg tccaaaact 
      tcttcatcg tgtcgctcg cagtcgccg acctcacag 
      tcgccatcc tcgtcatgc ccctgaacg ttatgctgt 
      cgatcttgg gacgatggg tgttcgaca tccacatct 
      gcaaaatgt ggctgactt cggacgtcc tctgctgca 
      cggcttcaa ttctgaatc tgtgtgcga ttgcgttgg 
      accgctttt gggccatca cagacccca ttaactacg 
      cgcaaaagc gcaccctga aaagggtcc tgttgatga 
      ttggaggcg tgtgggttt tgtcactag tgatcagtt 
      ctccgccac tgatcggct ggaacgact ggcctgaag 
      ccgacgagt tccacttga ttcaagagt gcaagttaa 
      cggaacggc aaggctatg tcatttact catcactgg 
      ggtcgttct tcatccctt tgttcatca tgacgatcg 
      tctacgttg agattttca tcgccacga agcgccgct 
      tgagagaga gggcacaag cctcgaaaa tcaacgcaa 
      tctcgcgga accaatcgg ctctgatca gcaacaaag 
      aagcacagc atgatcgcg agagtgtca gcagcgaaa 
      ccaatcata acgagcagc ccgaaacca acggcaagc 
      ccaaagaca agaagaaga aaaccaaga agaagcgaa 
      aagacgaca acgtcgaca acaacgcct atctcaaac 
      cgattctag tccatgaag attctctaa ctgacaatc 
      cggaaactt cctcaatct cgcaacggc ggggttcca 
      acccttgca cttccgaca accacaaaa tcacagttt 
      ccgagtcca cgacggtgt caaactcgc cccagctga 
      acaaggttt tgttgccgg aagccaccg ccacggtga 
      aaaagccgg gagtggttt accaattca tcgaagaga 
      aacaacgca tttccttgt cgaaggaga gacgcgcgg 
      cgcgaactc ttggaataa taatgggag tgtttgtgg 
      tgtgttggt tgccgttct tcctcatgt atgtaacga 
      tccctttct gccccagtt gttgcccct ccaagagac 
      tcaagaact ttatcactt ggttaggtt acattaatt 
      ccgtgctca atcccatta tttatacga tttttaatc 
      tcgactttc ggcgcgctt ttagaaagc ttctaggga 
      tgaagccgt gagtttccg gtatatccg tcgacaact 
      aattgataa ttcccgcgt tttttcaaa gactgattt 
      ctagagttg cattcggtg taaattaaa taaaattca 
      tgtttgtta tacagcatt ccaagtaat gtctatcat 
      cgaactaat ttgccgaat ctgttacga agtgttcga 
      gcccaacgc ttccgaacc ggtttggca aacttttac 
      gcttatttt tactgttaa tgttgaatg gcatcttca 
      aattgtaca tttgcttca gatcttgta cataaatat 
      tagaatact ctctcaatt gtacctgtg tgatattcg 
      ccctcttgc tattgcgtt aaatttacc tcagattca 
      catttttaa ctccaggct ttaaaaatg tgcatccac 
      attgcatat attttatac aagttactt caagcaaat 
      tttataaaa tctgttagg tatgggatg aagccgaaa 
      ctatctgta atcaagcta caccttcgc tcgtttcac 
      gctttttga ctcatcctg tatcacgtt ttgaatgta 
      tgataaata gaaaccaat aaaattcaa acgttttg
    • >TC004545 RB
      gttttgagc gagaaagcg cgcgtttaa cgaaactta 
      ttttcaact tggtgcgat gtggacgct tatttttag 
      ttgcgtttg agtacggac ctggaaacg gggaaaaat 
      gattggaga atgaatgga aatagtttt ccgtggagc 
      tccagtaaa gtgcatgca acgaaattt cctgcgaga 
      cctacaaat taaatttga cctatctaa ctaaataac 
      cctgatggc gaatggatg agtcggagt ccttcacaa 
      gatggacca attaggatt ttgccgcag aatccgcag 
      ttacggagc gtaatagag accggcgag attggataa 
      attgttggg agcttcccg aagatgtaa ttaagcttt 
      gcgaccatg tccgactca aacagcagc tgctccccc 
      gacctgccc ggcaacttt tcgacgatt ttcggccag 
      acggacgtc gcccaatgg gaagccctc ctcacaatc 
      gtcactctc accctcatc atcctcgtc acaatcgtc 
      ggcaacgtc ctcgtcatt ctcagcgtc ttcacctac 
      aaaccgctc cgaatcgtc caaaacttc ttcatcgtg 
      tcgctcgca gtcgccgac ctcacagtc gccatcctc 
      gtcatgccc ctgaacgtt atgctgtcg atcttggga 
      cgatgggtg ttcgacatc cacatctgc aaaatgtgg 
      ctgacttcg gacgtcctc tgctgcacg gcttcaatt 
      ctgaatctg tgtgcgatt gcgttggac cgcttttgg 
      gccatcaca gaccccatt aactacgcg caaaagcgc 
      accctgaaa agggtcctg ttgatgatt ggaggcgtg 
      tgggttttg tcactagtg atcagttct ccgccactg 
      atcggctgg aacgactgg cctgaagcc gacgagttc 
      cacttgatt caagagtgc aagttaacg gaacggcaa 
      ggctatgtc atttactca tcactgggg tcgttcttc 
      atccctttg ttcatcatg acgatcgtc tacgttgag 
      attttcatc gccacgaag cgccgcttg agagagagg 
      gcacaagcc tcgaaaatc aacgcaatc tcgcggaac 
      caatcggct ctgatcagc aacaaagaa gcacagcat 
      gatcgcgag agtgtcagc agcgaaacc aatcataac 
      gagcagccc gaaaccaac ggcaagccc aaagacaag 
      aagaagaaa accaagaag aagcgaaaa gacgacaac 
      gtcgacaac aacgcctat ctcaaaccg attctagtc 
      catgaagat tctctaact gacaatccg gaaacttcc 
      tcaatctcg caacggcgg ggttccaac ccttgcact 
      tccgacaac cacaaaatc acagtttcc gagtccacg 
      acggtgtca aactcgccc cagctgaac aaggttttg 
      ttgccggaa gccaccgcc acggtgaaa aagccggga 
      gtggtttac caattcatc gaagagaaa caacgcatt 
      tccttgtcg aaggagaga cgcgcggcg cgaactctt 
      ggaataata atgggagtg tttgtggtg tgttggttg 
      ccgttcttc ctcatgtat gtaacgatc cctttctgc 
      cccagttgt tgcccctcc aagagactc aagaacttt 
      atcacttgg ttaggttac attaattcc gtgctcaat 
      cccattatt tatacgatt tttaatctc gactttcgg 
      cgcgctttt agaaagctt ctagggatg aagccgtga 
      gtttccggt atatccgtc gacaactaa ttgataatt 
      cccgcgttt tttcaaaga ctgatttct agagttgca 
      ttcggtgta aattaaata aaattcatg tttgttata 
      cagcattcc aagtaatgt ctatcatcg aactaattt 
      gccgaatct gttacgaag tgttcgagc ccaacgctt 
      ccgaaccgg tttggcaaa cttttacgc ttattttta 
      ctgttaatg ttgaatggc atcttcaaa ttgtacatt 
      tgcttcaga tcttgtaca taaatatta gaatactct 
      ctcaattgt acctgtgtg atattcgcc ctcttgcta 
      ttgcgttaa atttacctc agattcaca tttttaact 
      ccaggcttt aaaaatgtg catccacat tgcatatat 
      tttatacaa gttacttca agcaaattt tataaaatc 
      tgttaggta tgggatgaa gccgaaact atctgtaat 
      caagctaca ccttcgctc gtttcacgc tttttgact 
      catcctgta tcacgtttt gaatgtatg ataaataga 
      aaccaataa aattcaaac gttttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY