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Gene information
General information
TC004532 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3054103..3055307
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004532
    iBeetle screen iB_00714

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 75.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 9

    wings not closed - Number of animals showing the phenotype: 9
    Fertility
    egg production: number no/almost no eggs -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004532 RA
      MLFLRKLVS PSPCVCVNF VRHAKRIVD PGIRRRKEL 
      LQLDESEEI DDFDDFETD FMEVHKSHK QHLREAEVE 
      REKMKYHIV KQRYFKEKF PNFLTWHDK EQIRYLYRT 
      DPNEWTIER LSESFPALP EVIKKIIKS NWDKPSQNK 
      ITNHDTAVQ NNWKLFKNG KMGDLPADL AQHLNKFTN 
      RALNLNQFK LPDFKTNVV TVGKKIGTE FSDIVTSYG 
      GSHQNEDEK LEIEAKTLP DKKSKSRKP VTLNQLQVK 
      LVKRAELGK QIDENEKCI VSSVKTEEE KIEVKHDLV 
      DLSHNKFQT GVQLHKKEE KDLSHLNYP EVITIPKSA 
      LKKGCIYKL NDCYYDDDG EFLYRVPGM SQ
    CDS sequence
    • >TC004532 RA
      atgttattt ctgagaaaa ctagtttca ccttctccc 
      tgcgtttgt gttaatttt gtccgacat gcgaagaga 
      atcgttgat cccggaata cgaagacgc aaggaatta 
      ttacaactc gacgaatcc gaagaaatt gacgatttt 
      gacgacttt gaaaccgat tttatggaa gtacacaag 
      tcccataaa caacacctt cgagaagca gaggttgag 
      agggaaaaa atgaaatat cacattgta aagcaacgg 
      tattttaaa gaaaagttt ccgaatttt ttaacttgg 
      catgataag gaacaaatt cggtatttg taccgtact 
      gatccgaat gagtggacg attgaacgg ttatctgag 
      agttttcca gcattgccg gaagtcatc aagaaaatt 
      atcaaatca aattgggac aaaccaagt cagaataaa 
      attacaaat cacgacact gcggtccaa aataactgg 
      aagttgttt aaaaacggg aaaatgggg gatttacct 
      gcggatttg gcccaacat ttgaataag tttacaaat 
      cgcgcactc aatttgaat cagtttaaa ttaccagac 
      tttaaaacc aatgtagtg actgttggg aaaaaaatt 
      ggaacggag ttttctgat attgtgact agttacggt 
      ggtagccac caaaatgag gatgaaaaa ctagaaata 
      gaagcaaaa actttgccc gataaaaaa tcaaaaagt 
      cgaaaacct gtcacactt aatcagctt caagtaaaa 
      ctggtcaaa agggcagaa ttaggtaag caaatcgat 
      gagaacgaa aaatgcata gtaagcagt gtgaagacg 
      gaggaagag aaaattgaa gtaaaacac gacttggtt 
      gatttgtcc cacaacaaa tttcaaact ggggtccaa 
      cttcacaaa aaagaggaa aaggatttg tctcatttg 
      aattacccg gaagttatc accataccc aaatctgcg 
      ctaaagaaa ggctgtatt tacaaactc aatgattgt 
      tactacgac gatgacggc gaatttttg tatcgagtc 
      ccaggaatg tcgcaataa
    mRNA sequence
    • >TC004532 RA
      aaattttta cgtccataa acctcactt ttttaatta 
      ataatttaa aacgatgtt atttctgag aaaactagt 
      ttcaccttc tccctgcgt ttgtgttaa ttttgtccg 
      acatgcgaa gagaatcgt tgatcccgg aatacgaag 
      acgcaagga attattaca actcgacga atccgaaga 
      aattgacga ttttgacga ctttgaaac cgattttat 
      ggaagtaca caagtccca taaacaaca ccttcgaga 
      agcagaggt tgagaggga aaaaatgaa atatcacat 
      tgtaaagca acggtattt taaagaaaa gtttccgaa 
      ttttttaac ttggcatga taaggaaca aattcggta 
      tttgtaccg tactgatcc gaatgagtg gacgattga 
      acggttatc tgagagttt tccagcatt gccggaagt 
      catcaagaa aattatcaa atcaaattg ggacaaacc 
      aagtcagaa taaaattac aaatcacga cactgcggt 
      ccaaaataa ctggaagtt gtttaaaaa cgggaaaat 
      gggggattt acctgcgga tttggccca acatttgaa 
      taagtttac aaatcgcgc actcaattt gaatcagtt 
      taaattacc agactttaa aaccaatgt agtgactgt 
      tgggaaaaa aattggaac ggagttttc tgatattgt 
      gactagtta cggtggtag ccaccaaaa tgaggatga 
      aaaactaga aatagaagc aaaaacttt gcccgataa 
      aaaatcaaa aagtcgaaa acctgtcac acttaatca 
      gcttcaagt aaaactggt caaaagggc agaattagg 
      taagcaaat cgatgagaa cgaaaaatg catagtaag 
      cagtgtgaa gacggagga agagaaaat tgaagtaaa 
      acacgactt ggttgattt gtcccacaa caaatttca 
      aactggggt ccaacttca caaaaaaga ggaaaagga 
      tttgtctca tttgaatta cccggaagt tatcaccat 
      acccaaatc tgcgctaaa gaaaggctg tatttacaa 
      actcaatga ttgttacta cgacgatga cggcgaatt 
      tttgtatcg agtcccagg aatgtcgca ataaataaa 
      agaaataaa attgtgtat ttttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY