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Gene information
General information
TC004513 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3580680..3581897
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004513
    iBeetle screen iB_00712

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax partially present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004513 RA
      MSSYSKHTE DSLPKVIIC GMTENNMPK LIVCDEKKL 
      QDDKVCTAQ CSQKSSRED VAPNLSQRL VNMQKQLVA 
      GNAGLTGMS RKLECDLLQ KNRTIENLQ KQLCQLQCE 
      MNNISKQNA ILNEKIEQA TGHQKPDAQ IPCGKNYAC 
      LESRLNEYT ENTKALEKQ LCEMESHVE AMKSALTTV 
      QAERANLEK SKNILMLCP PPMTCPRPV CPKPRHCSF 
      KGSISDQQL ANLDSGETT TIFKKFSQI WSNKLW
    CDS sequence
    • >TC004513 RA
      atgtcctcc tacagcaag cacactgaa gattcgctg 
      cccaaagtg ataatttgc ggcatgacg gagaacaac 
      atgcccaaa ctgatagtt tgcgatgag aaaaaatta 
      caggacgac aaagtatgc acagcgcag tgtagccag 
      aaaagttcc cgtgaagat gtggccccg aatctgtcc 
      caaagactc gtcaacatg cagaagcag ctcgtggcc 
      ggaaacgcc ggtcttacc ggaatgagt cgcaaactg 
      gaatgcgac ttattgcag aaaaaccga acgattgag 
      aacttgcaa aagcaactg tgccaactc cagtgcgaa 
      atgaacaac atttcgaaa cagaacgca atcctcaac 
      gaaaaaatc gaacaagcg actggacac caaaagccc 
      gacgcgcaa attccgtgc gggaaaaac tacgcctgt 
      ttggagagt cgtctaaac gagtacacg gaaaacacg 
      aaagctttg gaaaagcag ctgtgcgaa atggaaagt 
      catgtcgag gcgatgaaa agcgcactg acgacggtg 
      caagcggag agggccaat ttggaaaag agcaaaaat 
      attctaatg ctgtgtccg cctccgatg acttgtccg 
      cggccagtg tgccccaaa ccgcgccac tgcagcttc 
      aaggggagc atctccgac caacaactg gccaatttg 
      gacagtggc gaaacgacg actattttt aagaaattc 
      tcgcaaatt tggtcaaat aaattgtgg taa
    mRNA sequence
    • >TC004513 RA
      acagattat ggttgtata tgaattttc aaaagatta 
      tcattatta gaacttgtg aaacttcca atgtttcta 
      atgtgggat gtaacggaa cagtctgta tattgggaa 
      taccggaac tctagtgtg aacttatca gacgacgct 
      tcaaataga taattaatt tcatatttc atagaaact 
      gtcatcaat taaaaacat gtcaaaatt tcaagttta 
      agtccccga aatgtcctc ctacagcaa gcacactga 
      agattcgct gcccaaagt gataatttg cggcatgac 
      ggagaacaa catgcccaa actgatagt ttgcgatga 
      gaaaaaatt acaggacga caaagtatg cacagcgca 
      gtgtagcca gaaaagttc ccgtgaaga tgtggcccc 
      gaatctgtc ccaaagact cgtcaacat gcagaagca 
      gctcgtggc cggaaacgc cggtcttac cggaatgag 
      tcgcaaact ggaatgcga cttattgca gaaaaaccg 
      aacgattga gaacttgca aaagcaact gtgccaact 
      ccagtgcga aatgaacaa catttcgaa acagaacgc 
      aatcctcaa cgaaaaaat cgaacaagc gactggaca 
      ccaaaagcc cgacgcgca aattccgtg cgggaaaaa 
      ctacgcctg tttggagag tcgtctaaa cgagtacac 
      ggaaaacac gaaagcttt ggaaaagca gctgtgcga 
      aatggaaag tcatgtcga ggcgatgaa aagcgcact 
      gacgacggt gcaagcgga gagggccaa tttggaaaa 
      gagcaaaaa tattctaat gctgtgtcc gcctccgat 
      gacttgtcc gcggccagt gtgccccaa accgcgcca 
      ctgcagctt caaggggag catctccga ccaacaact 
      ggccaattt ggacagtgg cgaaacgac gactatttt 
      taagaaatt ctcgcaaat ttggtcaaa taaattgtg 
      gtaaagcgg ttgtggttt cattgagag agaaggagc 
      aagacacgc agacaaatg ttaattaat tttccattt 
      aaagagatt agagccatc catcaagtt gttccggcc 
      gtattgagg gttattcgg atttgttat tgacaccct 
      tcttttgaa ggattttct gccatcatt ggctgcact 
      ctgatggaa tgattaacg ctgaaaaac actgaattg 
      acacgttat cactaaacc atctatttt taa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY