| General information | |||
| TC004511 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 3607477..3612637 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004511 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00711 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC004511 RA
MDSDSEDDM LHYSHQKLS SLIRQKGNK NAAIKEYLE NLNYHPIKD FASGLEWFN VSEPLSFSK HLKGKIVVL DFFTYCCIN CMHIIPDLR EVEKEFSVE DGLVVIGVH SAKFENEKV SSNILAAVQ RYNITHPVV NDFNSQMWK NCEVSCWPT LLILGPNAN PIVMLMGEG HKENLRTYI RAALEYYKE KNQISDHQI PFKSAYHLL PDLKGPLLF PGKITNFVT KNQEEILAI SDTGNNRIL ILKSDGTIL HQVGSGEIG FRDGSFTQC EFNAPQGLV FQNENTLFV ADTENHAIR KIDLAKKTV ESVVGSGVQ GHDRVGGKQ WTQQVISSP WDLCIFRTP DMDMSFYPE GNPPIREIL IIAMAGTHQ IWALFLENT VWWKCKKYT AGTCLSIAG SGREENKNN AYPHAAGFA QPSGLALCS KNKEVYIAD SESSSVRRL SLVDGKVMP VVGGDRNPN NLFAFGDKD GTLFDAKLQ HLLGLAMSK DEDLLFVAD TYNHKLKKV KIVENSVST VTLPASDTT DGAKNHPFS EPAGLCVSS DGRKIYLAD TNNHCVKVL TLGSDYSVK SINKLNLNL GEKTANDEE IKHQILEYP SLTINNQGG KVIFSLNIE FANGLKLTE EAPQKWSVK LPDVTWSCV PSSGDNLKN IDVVVSVPN RGQIDFVFD LVTCTVETC LPKSFIVRV PIDLKSNVS TSVNANIKV VLDPNNIQV N
- >TC004511 RA
atggattca gacagcgaa gacgatatg ctgcattat tcccaccag aaattatcg agtctgata agacaaaaa gggaataaa aatgcagca attaaagaa tatttggaa aacttgaat tatcacccc atcaaggat ttcgcgtca ggtcttgaa tggtttaat gtttccgaa ccgttgtcc ttttcgaag catttgaag ggtaaaatc gtcgtgttg gactttttt acgtattgt tgtatcaac tgtatgcac attatcccc gatttgcgc gaagttgag aaggaattt tcggtggaa gatgggcta gttgtgata ggtgtccac agcgcaaag tttgaaaat gagaaagtg tcctcgaat attctcgct gcggtgcaa cgttacaac atcacccat cctgtcgtc aacgatttc aattcccaa atgtggaag aactgcgag gttagttgt tggccgact ttacttatt ttgggacca aatgccaac ccgatcgtc atgctgatg ggggaaggc cacaaggag aatttacga acttacatc agggccgct ttagagtat tacaaggag aaaaatcaa atttctgac catcaaatt ccgttcaag tcggcttat catttactc cctgacttg aaaggcccc ctattattc ccgggaaaa attactaat tttgttacc aaaaaccag gaagaaatt ttagccata tcagacact ggtaacaat cgaatcctt attctaaaa tcagatggg accatttta caccaagtt ggtagcgga gaaatcggt tttcgtgat ggtagtttc acacaatgt gaatttaac gctccccag ggcttagtt ttccaaaac gagaacact cttttcgtt gctgatacg gaaaatcac gcaattcgc aaaattgat ttagcgaaa aaaactgtt gagagtgtc gttgggtca ggtgtgcaa ggtcatgac agagttggc gggaaacaa tggacgcaa caagtcata tcatcacct tgggatttg tgtattttt cggacgcct gacatggat atgagtttt tatcccgag gggaatccc ccaattagg gagatttta attattgca atggctgga acgcaccag atttgggct ttatttctt gagaacacg gtttggtgg aaatgtaaa aaatacact gctgggact tgtctaagt attgctggg tctggacgc gaagagaat aaaaataat gcatatcct catgctgct gggtttgca caaccctct ggattggct ttgtgcagc aaaaacaag gaggtttat atcgctgat agtgaaagt tcaagtgtg agacgcttg tcgcttgtg gatggaaaa gttatgcct gtggttgga ggggatagg aacccaaat aatttgttc gcgtttggg gacaaagac gggaccttg tttgacgcc aaactgcaa catcttctc ggccttgca atgtcaaaa gacgaagat ttgcttttc gtggccgat acgtacaac cacaagttg aagaaagtt aagattgtt gaaaattcc gtgtctacg gtcaccctc ccggcttcc gacacgaca gacggggct aaaaatcac ccatttagc gagcctgca ggtttgtgt gtttcttca gacggtcga aaaatatac ctagcggac actaacaac cactgtgtt aaagttcta actctcggt tcggattat tccgtcaaa tctataaat aaattaaat ttgaatttg ggggaaaag acagcaaat gacgaggaa attaaacac caaattctt gaatatcca agtctcacg attaataat caaggcggg aaagtgatt ttctcgcta aatatcgaa tttgcaaac gggttgaaa ttgacagag gaagcgccc caaaaatgg tcagtaaaa ttaccggat gttacatgg tcttgtgtg ccgagtagt ggcgataat ttgaaaaat attgacgtg gtggttagt gtcccgaat cgaggtcaa attgatttt gtttttgat ttggttact tgtactgtt gagacgtgc ttacccaaa agctttatt gtccgagtg cctattgac ttgaaaagt aatgtttcg acgagtgtt aatgcgaat attaaggtg gttctagac ccgaataat attcaagtg aactaa
- >TC004511 RA
aacgccaga aggtttatt gcaaattta tgggattcc gaaaaataa atagaaaaa cgaaaaaaa attatttca gtatatttc gttaatgtt ttaaattgt tgacgtgtt tttatcact tccctttta attttatca aagtgtgtg cattttttg agtcaacaa cggtttttg tgtgagcca gtacttgtt ttgagtggt ggttacaca gttaatgcg acttttgaa ccgtttttt accctccaa aatttggtt tagacggtt atttcgctc aagaatgga ttcagacag cgaagacga tatgctgca ttattccca ccagaaatt atcgagtct gataagaca aaaagggaa taaaaatgc agcaattaa agaatattt ggaaaactt gaattatca ccccatcaa ggatttcgc gtcaggtct tgaatggtt taatgtttc cgaaccgtt gtccttttc gaagcattt gaagggtaa aatcgtcgt gttggactt ttttacgta ttgttgtat caactgtat gcacattat ccccgattt gcgcgaagt tgagaagga attttcggt ggaagatgg gctagttgt gataggtgt ccacagcgc aaagtttga aaatgagaa agtgtcctc gaatattct cgctgcggt gcaacgtta caacatcac ccatcctgt cgtcaacga tttcaattc ccaaatgtg gaagaactg cgaggttag ttgttggcc gactttact tattttggg accaaatgc caacccgat cgtcatgct gatggggga aggccacaa ggagaattt acgaactta catcagggc cgctttaga gtattacaa ggagaaaaa tcaaatttc tgaccatca aattccgtt caagtcggc ttatcattt actccctga cttgaaagg ccccctatt attcccggg aaaaattac taattttgt taccaaaaa ccaggaaga aattttagc catatcaga cactggtaa caatcgaat ccttattct aaaatcaga tgggaccat tttacacca agttggtag cggagaaat cggttttcg tgatggtag tttcacaca atgtgaatt taacgctcc ccagggctt agttttcca aaacgagaa cactctttt cgttgctga tacggaaaa tcacgcaat tcgcaaaat tgatttagc gaaaaaaac tgttgagag tgtcgttgg gtcaggtgt gcaaggtca tgacagagt tggcgggaa acaatggac gcaacaagt catatcatc accttggga tttgtgtat ttttcggac gcctgacat ggatatgag tttttatcc cgaggggaa tcccccaat tagggagat tttaattat tgcaatggc tggaacgca ccagatttg ggctttatt tcttgagaa cacggtttg gtggaaatg taaaaaata cactgctgg gacttgtct aagtattgc tgggtctgg acgcgaaga gaataaaaa taatgcata tcctcatgc tgctgggtt tgcacaacc ctctggatt ggctttgtg cagcaaaaa caaggaggt ttatatcgc tgatagtga aagttcaag tgtgagacg cttgtcgct tgtggatgg aaaagttat gcctgtggt tggagggga taggaaccc aaataattt gttcgcgtt tggggacaa agacgggac cttgtttga cgccaaact gcaacatct tctcggcct tgcaatgtc aaaagacga agatttgct tttcgtggc cgatacgta caaccacaa gttgaagaa agttaagat tgttgaaaa ttccgtgtc tacggtcac cctcccggc ttccgacac gacagacgg ggctaaaaa tcacccatt tagcgagcc tgcaggttt gtgtgtttc ttcagacgg tcgaaaaat atacctagc ggacactaa caaccactg tgttaaagt tctaactct cggttcgga ttattccgt caaatctat aaataaatt aaatttgaa tttggggga aaagacagc aaatgacga ggaaattaa acaccaaat tcttgaata tccaagtct cacgattaa taatcaagg cgggaaagt gattttctc gctaaatat cgaatttgc aaacgggtt gaaattgac agaggaagc gccccaaaa atggtcagt aaaattacc ggatgttac atggtcttg tgtgccgag tagtggcga taatttgaa aaatattga cgtggtggt tagtgtccc gaatcgagg tcaaattga ttttgtttt tgatttggt tacttgtac tgttgagac gtgcttacc caaaagctt tattgtccg agtgcctat tgacttgaa aagtaatgt ttcgacgag tgttaatgc gaatattaa ggtggttct agacccgaa taatattca agtgaacta agagttttt tattttgca ccaacattt aagtactaa aaagtgatt ataattatt tgttgcaat aaatattac attaacac
