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Gene information
General information
TC004511 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3607477..3612637
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004511
    iBeetle screen iB_00711

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004511 RA
      MDSDSEDDM LHYSHQKLS SLIRQKGNK NAAIKEYLE 
NLNYHPIKD FASGLEWFN VSEPLSFSK HLKGKIVVL 
DFFTYCCIN CMHIIPDLR EVEKEFSVE DGLVVIGVH 
SAKFENEKV SSNILAAVQ RYNITHPVV NDFNSQMWK 
NCEVSCWPT LLILGPNAN PIVMLMGEG HKENLRTYI 
RAALEYYKE KNQISDHQI PFKSAYHLL PDLKGPLLF 
PGKITNFVT KNQEEILAI SDTGNNRIL ILKSDGTIL 
HQVGSGEIG FRDGSFTQC EFNAPQGLV FQNENTLFV 
ADTENHAIR KIDLAKKTV ESVVGSGVQ GHDRVGGKQ 
WTQQVISSP WDLCIFRTP DMDMSFYPE GNPPIREIL 
IIAMAGTHQ IWALFLENT VWWKCKKYT AGTCLSIAG 
SGREENKNN AYPHAAGFA QPSGLALCS KNKEVYIAD 
SESSSVRRL SLVDGKVMP VVGGDRNPN NLFAFGDKD 
GTLFDAKLQ HLLGLAMSK DEDLLFVAD TYNHKLKKV 
KIVENSVST VTLPASDTT DGAKNHPFS EPAGLCVSS 
DGRKIYLAD TNNHCVKVL TLGSDYSVK SINKLNLNL 
GEKTANDEE IKHQILEYP SLTINNQGG KVIFSLNIE 
FANGLKLTE EAPQKWSVK LPDVTWSCV PSSGDNLKN 
IDVVVSVPN RGQIDFVFD LVTCTVETC LPKSFIVRV 
PIDLKSNVS TSVNANIKV VLDPNNIQV N
    CDS sequence
    • >TC004511 RA
      atggattca gacagcgaa gacgatatg ctgcattat 
tcccaccag aaattatcg agtctgata agacaaaaa 
gggaataaa aatgcagca attaaagaa tatttggaa 
aacttgaat tatcacccc atcaaggat ttcgcgtca 
ggtcttgaa tggtttaat gtttccgaa ccgttgtcc 
ttttcgaag catttgaag ggtaaaatc gtcgtgttg 
gactttttt acgtattgt tgtatcaac tgtatgcac 
attatcccc gatttgcgc gaagttgag aaggaattt 
tcggtggaa gatgggcta gttgtgata ggtgtccac 
agcgcaaag tttgaaaat gagaaagtg tcctcgaat 
attctcgct gcggtgcaa cgttacaac atcacccat 
cctgtcgtc aacgatttc aattcccaa atgtggaag 
aactgcgag gttagttgt tggccgact ttacttatt 
ttgggacca aatgccaac ccgatcgtc atgctgatg 
ggggaaggc cacaaggag aatttacga acttacatc 
agggccgct ttagagtat tacaaggag aaaaatcaa 
atttctgac catcaaatt ccgttcaag tcggcttat 
catttactc cctgacttg aaaggcccc ctattattc 
ccgggaaaa attactaat tttgttacc aaaaaccag 
gaagaaatt ttagccata tcagacact ggtaacaat 
cgaatcctt attctaaaa tcagatggg accatttta 
caccaagtt ggtagcgga gaaatcggt tttcgtgat 
ggtagtttc acacaatgt gaatttaac gctccccag 
ggcttagtt ttccaaaac gagaacact cttttcgtt 
gctgatacg gaaaatcac gcaattcgc aaaattgat 
ttagcgaaa aaaactgtt gagagtgtc gttgggtca 
ggtgtgcaa ggtcatgac agagttggc gggaaacaa 
tggacgcaa caagtcata tcatcacct tgggatttg 
tgtattttt cggacgcct gacatggat atgagtttt 
tatcccgag gggaatccc ccaattagg gagatttta 
attattgca atggctgga acgcaccag atttgggct 
ttatttctt gagaacacg gtttggtgg aaatgtaaa 
aaatacact gctgggact tgtctaagt attgctggg 
tctggacgc gaagagaat aaaaataat gcatatcct 
catgctgct gggtttgca caaccctct ggattggct 
ttgtgcagc aaaaacaag gaggtttat atcgctgat 
agtgaaagt tcaagtgtg agacgcttg tcgcttgtg 
gatggaaaa gttatgcct gtggttgga ggggatagg 
aacccaaat aatttgttc gcgtttggg gacaaagac 
gggaccttg tttgacgcc aaactgcaa catcttctc 
ggccttgca atgtcaaaa gacgaagat ttgcttttc 
gtggccgat acgtacaac cacaagttg aagaaagtt 
aagattgtt gaaaattcc gtgtctacg gtcaccctc 
ccggcttcc gacacgaca gacggggct aaaaatcac 
ccatttagc gagcctgca ggtttgtgt gtttcttca 
gacggtcga aaaatatac ctagcggac actaacaac 
cactgtgtt aaagttcta actctcggt tcggattat 
tccgtcaaa tctataaat aaattaaat ttgaatttg 
ggggaaaag acagcaaat gacgaggaa attaaacac 
caaattctt gaatatcca agtctcacg attaataat 
caaggcggg aaagtgatt ttctcgcta aatatcgaa 
tttgcaaac gggttgaaa ttgacagag gaagcgccc 
caaaaatgg tcagtaaaa ttaccggat gttacatgg 
tcttgtgtg ccgagtagt ggcgataat ttgaaaaat 
attgacgtg gtggttagt gtcccgaat cgaggtcaa 
attgatttt gtttttgat ttggttact tgtactgtt 
gagacgtgc ttacccaaa agctttatt gtccgagtg 
cctattgac ttgaaaagt aatgtttcg acgagtgtt 
aatgcgaat attaaggtg gttctagac ccgaataat 
attcaagtg aactaa
    mRNA sequence
    • >TC004511 RA
      aacgccaga aggtttatt gcaaattta tgggattcc 
gaaaaataa atagaaaaa cgaaaaaaa attatttca 
gtatatttc gttaatgtt ttaaattgt tgacgtgtt 
tttatcact tccctttta attttatca aagtgtgtg 
cattttttg agtcaacaa cggtttttg tgtgagcca 
gtacttgtt ttgagtggt ggttacaca gttaatgcg 
acttttgaa ccgtttttt accctccaa aatttggtt 
tagacggtt atttcgctc aagaatgga ttcagacag 
cgaagacga tatgctgca ttattccca ccagaaatt 
atcgagtct gataagaca aaaagggaa taaaaatgc 
agcaattaa agaatattt ggaaaactt gaattatca 
ccccatcaa ggatttcgc gtcaggtct tgaatggtt 
taatgtttc cgaaccgtt gtccttttc gaagcattt 
gaagggtaa aatcgtcgt gttggactt ttttacgta 
ttgttgtat caactgtat gcacattat ccccgattt 
gcgcgaagt tgagaagga attttcggt ggaagatgg 
gctagttgt gataggtgt ccacagcgc aaagtttga 
aaatgagaa agtgtcctc gaatattct cgctgcggt 
gcaacgtta caacatcac ccatcctgt cgtcaacga 
tttcaattc ccaaatgtg gaagaactg cgaggttag 
ttgttggcc gactttact tattttggg accaaatgc 
caacccgat cgtcatgct gatggggga aggccacaa 
ggagaattt acgaactta catcagggc cgctttaga 
gtattacaa ggagaaaaa tcaaatttc tgaccatca 
aattccgtt caagtcggc ttatcattt actccctga 
cttgaaagg ccccctatt attcccggg aaaaattac 
taattttgt taccaaaaa ccaggaaga aattttagc 
catatcaga cactggtaa caatcgaat ccttattct 
aaaatcaga tgggaccat tttacacca agttggtag 
cggagaaat cggttttcg tgatggtag tttcacaca 
atgtgaatt taacgctcc ccagggctt agttttcca 
aaacgagaa cactctttt cgttgctga tacggaaaa 
tcacgcaat tcgcaaaat tgatttagc gaaaaaaac 
tgttgagag tgtcgttgg gtcaggtgt gcaaggtca 
tgacagagt tggcgggaa acaatggac gcaacaagt 
catatcatc accttggga tttgtgtat ttttcggac 
gcctgacat ggatatgag tttttatcc cgaggggaa 
tcccccaat tagggagat tttaattat tgcaatggc 
tggaacgca ccagatttg ggctttatt tcttgagaa 
cacggtttg gtggaaatg taaaaaata cactgctgg 
gacttgtct aagtattgc tgggtctgg acgcgaaga 
gaataaaaa taatgcata tcctcatgc tgctgggtt 
tgcacaacc ctctggatt ggctttgtg cagcaaaaa 
caaggaggt ttatatcgc tgatagtga aagttcaag 
tgtgagacg cttgtcgct tgtggatgg aaaagttat 
gcctgtggt tggagggga taggaaccc aaataattt 
gttcgcgtt tggggacaa agacgggac cttgtttga 
cgccaaact gcaacatct tctcggcct tgcaatgtc 
aaaagacga agatttgct tttcgtggc cgatacgta 
caaccacaa gttgaagaa agttaagat tgttgaaaa 
ttccgtgtc tacggtcac cctcccggc ttccgacac 
gacagacgg ggctaaaaa tcacccatt tagcgagcc 
tgcaggttt gtgtgtttc ttcagacgg tcgaaaaat 
atacctagc ggacactaa caaccactg tgttaaagt 
tctaactct cggttcgga ttattccgt caaatctat 
aaataaatt aaatttgaa tttggggga aaagacagc 
aaatgacga ggaaattaa acaccaaat tcttgaata 
tccaagtct cacgattaa taatcaagg cgggaaagt 
gattttctc gctaaatat cgaatttgc aaacgggtt 
gaaattgac agaggaagc gccccaaaa atggtcagt 
aaaattacc ggatgttac atggtcttg tgtgccgag 
tagtggcga taatttgaa aaatattga cgtggtggt 
tagtgtccc gaatcgagg tcaaattga ttttgtttt 
tgatttggt tacttgtac tgttgagac gtgcttacc 
caaaagctt tattgtccg agtgcctat tgacttgaa 
aagtaatgt ttcgacgag tgttaatgc gaatattaa 
ggtggttct agacccgaa taatattca agtgaacta 
agagttttt tattttgca ccaacattt aagtactaa 
aaagtgatt ataattatt tgttgcaat aaatattac 
attaacac
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