Status
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Gene information
General information
TC004511 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3607477..3612637
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004511
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00711

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004511 RA
      MDSDSEDDM LHYSHQKLS SLIRQKGNK NAAIKEYLE 
      NLNYHPIKD FASGLEWFN VSEPLSFSK HLKGKIVVL 
      DFFTYCCIN CMHIIPDLR EVEKEFSVE DGLVVIGVH 
      SAKFENEKV SSNILAAVQ RYNITHPVV NDFNSQMWK 
      NCEVSCWPT LLILGPNAN PIVMLMGEG HKENLRTYI 
      RAALEYYKE KNQISDHQI PFKSAYHLL PDLKGPLLF 
      PGKITNFVT KNQEEILAI SDTGNNRIL ILKSDGTIL 
      HQVGSGEIG FRDGSFTQC EFNAPQGLV FQNENTLFV 
      ADTENHAIR KIDLAKKTV ESVVGSGVQ GHDRVGGKQ 
      WTQQVISSP WDLCIFRTP DMDMSFYPE GNPPIREIL 
      IIAMAGTHQ IWALFLENT VWWKCKKYT AGTCLSIAG 
      SGREENKNN AYPHAAGFA QPSGLALCS KNKEVYIAD 
      SESSSVRRL SLVDGKVMP VVGGDRNPN NLFAFGDKD 
      GTLFDAKLQ HLLGLAMSK DEDLLFVAD TYNHKLKKV 
      KIVENSVST VTLPASDTT DGAKNHPFS EPAGLCVSS 
      DGRKIYLAD TNNHCVKVL TLGSDYSVK SINKLNLNL 
      GEKTANDEE IKHQILEYP SLTINNQGG KVIFSLNIE 
      FANGLKLTE EAPQKWSVK LPDVTWSCV PSSGDNLKN 
      IDVVVSVPN RGQIDFVFD LVTCTVETC LPKSFIVRV 
      PIDLKSNVS TSVNANIKV VLDPNNIQV N
    CDS sequence
    • >TC004511 RA
      atggattca gacagcgaa gacgatatg ctgcattat 
      tcccaccag aaattatcg agtctgata agacaaaaa 
      gggaataaa aatgcagca attaaagaa tatttggaa 
      aacttgaat tatcacccc atcaaggat ttcgcgtca 
      ggtcttgaa tggtttaat gtttccgaa ccgttgtcc 
      ttttcgaag catttgaag ggtaaaatc gtcgtgttg 
      gactttttt acgtattgt tgtatcaac tgtatgcac 
      attatcccc gatttgcgc gaagttgag aaggaattt 
      tcggtggaa gatgggcta gttgtgata ggtgtccac 
      agcgcaaag tttgaaaat gagaaagtg tcctcgaat 
      attctcgct gcggtgcaa cgttacaac atcacccat 
      cctgtcgtc aacgatttc aattcccaa atgtggaag 
      aactgcgag gttagttgt tggccgact ttacttatt 
      ttgggacca aatgccaac ccgatcgtc atgctgatg 
      ggggaaggc cacaaggag aatttacga acttacatc 
      agggccgct ttagagtat tacaaggag aaaaatcaa 
      atttctgac catcaaatt ccgttcaag tcggcttat 
      catttactc cctgacttg aaaggcccc ctattattc 
      ccgggaaaa attactaat tttgttacc aaaaaccag 
      gaagaaatt ttagccata tcagacact ggtaacaat 
      cgaatcctt attctaaaa tcagatggg accatttta 
      caccaagtt ggtagcgga gaaatcggt tttcgtgat 
      ggtagtttc acacaatgt gaatttaac gctccccag 
      ggcttagtt ttccaaaac gagaacact cttttcgtt 
      gctgatacg gaaaatcac gcaattcgc aaaattgat 
      ttagcgaaa aaaactgtt gagagtgtc gttgggtca 
      ggtgtgcaa ggtcatgac agagttggc gggaaacaa 
      tggacgcaa caagtcata tcatcacct tgggatttg 
      tgtattttt cggacgcct gacatggat atgagtttt 
      tatcccgag gggaatccc ccaattagg gagatttta 
      attattgca atggctgga acgcaccag atttgggct 
      ttatttctt gagaacacg gtttggtgg aaatgtaaa 
      aaatacact gctgggact tgtctaagt attgctggg 
      tctggacgc gaagagaat aaaaataat gcatatcct 
      catgctgct gggtttgca caaccctct ggattggct 
      ttgtgcagc aaaaacaag gaggtttat atcgctgat 
      agtgaaagt tcaagtgtg agacgcttg tcgcttgtg 
      gatggaaaa gttatgcct gtggttgga ggggatagg 
      aacccaaat aatttgttc gcgtttggg gacaaagac 
      gggaccttg tttgacgcc aaactgcaa catcttctc 
      ggccttgca atgtcaaaa gacgaagat ttgcttttc 
      gtggccgat acgtacaac cacaagttg aagaaagtt 
      aagattgtt gaaaattcc gtgtctacg gtcaccctc 
      ccggcttcc gacacgaca gacggggct aaaaatcac 
      ccatttagc gagcctgca ggtttgtgt gtttcttca 
      gacggtcga aaaatatac ctagcggac actaacaac 
      cactgtgtt aaagttcta actctcggt tcggattat 
      tccgtcaaa tctataaat aaattaaat ttgaatttg 
      ggggaaaag acagcaaat gacgaggaa attaaacac 
      caaattctt gaatatcca agtctcacg attaataat 
      caaggcggg aaagtgatt ttctcgcta aatatcgaa 
      tttgcaaac gggttgaaa ttgacagag gaagcgccc 
      caaaaatgg tcagtaaaa ttaccggat gttacatgg 
      tcttgtgtg ccgagtagt ggcgataat ttgaaaaat 
      attgacgtg gtggttagt gtcccgaat cgaggtcaa 
      attgatttt gtttttgat ttggttact tgtactgtt 
      gagacgtgc ttacccaaa agctttatt gtccgagtg 
      cctattgac ttgaaaagt aatgtttcg acgagtgtt 
      aatgcgaat attaaggtg gttctagac ccgaataat 
      attcaagtg aactaa
    mRNA sequence
    • >TC004511 RA
      aacgccaga aggtttatt gcaaattta tgggattcc 
      gaaaaataa atagaaaaa cgaaaaaaa attatttca 
      gtatatttc gttaatgtt ttaaattgt tgacgtgtt 
      tttatcact tccctttta attttatca aagtgtgtg 
      cattttttg agtcaacaa cggtttttg tgtgagcca 
      gtacttgtt ttgagtggt ggttacaca gttaatgcg 
      acttttgaa ccgtttttt accctccaa aatttggtt 
      tagacggtt atttcgctc aagaatgga ttcagacag 
      cgaagacga tatgctgca ttattccca ccagaaatt 
      atcgagtct gataagaca aaaagggaa taaaaatgc 
      agcaattaa agaatattt ggaaaactt gaattatca 
      ccccatcaa ggatttcgc gtcaggtct tgaatggtt 
      taatgtttc cgaaccgtt gtccttttc gaagcattt 
      gaagggtaa aatcgtcgt gttggactt ttttacgta 
      ttgttgtat caactgtat gcacattat ccccgattt 
      gcgcgaagt tgagaagga attttcggt ggaagatgg 
      gctagttgt gataggtgt ccacagcgc aaagtttga 
      aaatgagaa agtgtcctc gaatattct cgctgcggt 
      gcaacgtta caacatcac ccatcctgt cgtcaacga 
      tttcaattc ccaaatgtg gaagaactg cgaggttag 
      ttgttggcc gactttact tattttggg accaaatgc 
      caacccgat cgtcatgct gatggggga aggccacaa 
      ggagaattt acgaactta catcagggc cgctttaga 
      gtattacaa ggagaaaaa tcaaatttc tgaccatca 
      aattccgtt caagtcggc ttatcattt actccctga 
      cttgaaagg ccccctatt attcccggg aaaaattac 
      taattttgt taccaaaaa ccaggaaga aattttagc 
      catatcaga cactggtaa caatcgaat ccttattct 
      aaaatcaga tgggaccat tttacacca agttggtag 
      cggagaaat cggttttcg tgatggtag tttcacaca 
      atgtgaatt taacgctcc ccagggctt agttttcca 
      aaacgagaa cactctttt cgttgctga tacggaaaa 
      tcacgcaat tcgcaaaat tgatttagc gaaaaaaac 
      tgttgagag tgtcgttgg gtcaggtgt gcaaggtca 
      tgacagagt tggcgggaa acaatggac gcaacaagt 
      catatcatc accttggga tttgtgtat ttttcggac 
      gcctgacat ggatatgag tttttatcc cgaggggaa 
      tcccccaat tagggagat tttaattat tgcaatggc 
      tggaacgca ccagatttg ggctttatt tcttgagaa 
      cacggtttg gtggaaatg taaaaaata cactgctgg 
      gacttgtct aagtattgc tgggtctgg acgcgaaga 
      gaataaaaa taatgcata tcctcatgc tgctgggtt 
      tgcacaacc ctctggatt ggctttgtg cagcaaaaa 
      caaggaggt ttatatcgc tgatagtga aagttcaag 
      tgtgagacg cttgtcgct tgtggatgg aaaagttat 
      gcctgtggt tggagggga taggaaccc aaataattt 
      gttcgcgtt tggggacaa agacgggac cttgtttga 
      cgccaaact gcaacatct tctcggcct tgcaatgtc 
      aaaagacga agatttgct tttcgtggc cgatacgta 
      caaccacaa gttgaagaa agttaagat tgttgaaaa 
      ttccgtgtc tacggtcac cctcccggc ttccgacac 
      gacagacgg ggctaaaaa tcacccatt tagcgagcc 
      tgcaggttt gtgtgtttc ttcagacgg tcgaaaaat 
      atacctagc ggacactaa caaccactg tgttaaagt 
      tctaactct cggttcgga ttattccgt caaatctat 
      aaataaatt aaatttgaa tttggggga aaagacagc 
      aaatgacga ggaaattaa acaccaaat tcttgaata 
      tccaagtct cacgattaa taatcaagg cgggaaagt 
      gattttctc gctaaatat cgaatttgc aaacgggtt 
      gaaattgac agaggaagc gccccaaaa atggtcagt 
      aaaattacc ggatgttac atggtcttg tgtgccgag 
      tagtggcga taatttgaa aaatattga cgtggtggt 
      tagtgtccc gaatcgagg tcaaattga ttttgtttt 
      tgatttggt tacttgtac tgttgagac gtgcttacc 
      caaaagctt tattgtccg agtgcctat tgacttgaa 
      aagtaatgt ttcgacgag tgttaatgc gaatattaa 
      ggtggttct agacccgaa taatattca agtgaacta 
      agagttttt tattttgca ccaacattt aagtactaa 
      aaagtgatt ataattatt tgttgcaat aaatattac 
      attaacac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY