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Gene information
General information
TC004510 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3621510..3622927
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004510
    iBeetle screen iB_00710

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004510 RA
      MASVPKVKF NNGLEIPIF GLGTWKSKP GQVAQAVKD 
      AIDIGYRHI DCAHVYGNE KEVGDAVVS KISQGVVKR 
      EDLFITSKL WNTFHRPDL VEGAIKQTL SDLGIEYLD 
      LYLIHWPLA YKEGGPLFP ADPQGKILF SDVDYVDTW 
      KAMEQLVHK GLTKSIGIS NFNKRQIER ILSVATITP 
      VTNQVECHP YLNQSKLIE FCKSKNITV TGYSPLGSP 
      DRPWAKPDE PQLLDDPKL KELATKYKK TPAQIVLRY 
      QVQRGVITI PKSVTKSRI QENFNIFDF ELTADDVKY 
      LNTFDCNGR FCPMTAGLG HSHHPFQND DY
    CDS sequence
    • >TC004510 RA
      atggcaagt gtcccgaaa gtaaaattc aacaacggc 
      ttagaaatc cccattttt ggcttgggg acatggaag 
      tccaaacca ggacaagta gctcaagct gtgaaggat 
      gccatcgat attggctat cgccacatt gattgtgct 
      catgtttat ggcaatgag aaagaagtt ggagatgct 
      gtcgtttct aaaatcagc caaggagtc gtaaaacgc 
      gaagacctc ttcattaca agcaaattg tggaatact 
      ttccaccgc cctgatttg gtggagggc gctattaag 
      cagacgttg tccgattta gggattgag tatctcgac 
      ttgtaccta atccattgg ccccttgct tacaaggaa 
      ggcgggccg ctcttcccc gccgacccc caaggcaaa 
      atcctcttc agcgacgtc gattacgtc gacacttgg 
      aaagcgatg gagcaactg gtccacaaa ggcctaacc 
      aaatccatc ggcatttcc aatttcaac aaacgccaa 
      attgagcga attttatca gtcgctaca attacccca 
      gtcactaat caggtcgag tgccaccct tatctcaat 
      caaagcaaa ttgattgag ttctgcaaa tcgaaaaac 
      atcaccgtg accggttac agccccttg gggtcgccc 
      gacagaccc tgggcgaag cccgacgag ccccagtta 
      ctggatgac cccaaactt aaggaactc gcgacgaaa 
      tataaaaaa actccagcg caaatcgtg ctgagatac 
      caggtgcaa agaggggtc atcaccatc cccaagtcg 
      gtcacaaag tcgcgaatt caggagaat ttcaatatt 
      tttgatttt gaactgacc gctgatgat gttaaatat 
      ttgaacacg tttgactgc aatggccgg ttttgccca 
      atgactgcg gggctgggg cactcacac cacccattc 
      caaaacgat gattattaa
    mRNA sequence
    • >TC004510 RA
      cgtggcaag tgacatgtt tgtgttttg ccgcttctt 
      gagtacagt taattattt agcgaagga tatcagtgg 
      aaaagtaaa tttaattaa atcttcgcc tattcagtc 
      tctgataga taacatggc aagtgtccc gaaagtaaa 
      attcaacaa cggcttaga aatccccat ttttggctt 
      ggggacatg gaagtccaa accaggaca agtagctca 
      agctgtgaa ggatgccat cgatattgg ctatcgcca 
      cattgattg tgctcatgt ttatggcaa tgagaaaga 
      agttggaga tgctgtcgt ttctaaaat cagccaagg 
      agtcgtaaa acgcgaaga cctcttcat tacaagcaa 
      attgtggaa tactttcca ccgccctga tttggtgga 
      gggcgctat taagcagac gttgtccga tttagggat 
      tgagtatct cgacttgta cctaatcca ttggcccct 
      tgcttacaa ggaaggcgg gccgctctt ccccgccga 
      cccccaagg caaaatcct cttcagcga cgtcgatta 
      cgtcgacac ttggaaagc gatggagca actggtcca 
      caaaggcct aaccaaatc catcggcat ttccaattt 
      caacaaacg ccaaattga gcgaatttt atcagtcgc 
      tacaattac cccagtcac taatcaggt cgagtgcca 
      cccttatct caatcaaag caaattgat tgagttctg 
      caaatcgaa aaacatcac cgtgaccgg ttacagccc 
      cttggggtc gcccgacag accctgggc gaagcccga 
      cgagcccca gttactgga tgaccccaa acttaagga 
      actcgcgac gaaatataa aaaaactcc agcgcaaat 
      cgtgctgag ataccaggt gcaaagagg ggtcatcac 
      catccccaa gtcggtcac aaagtcgcg aattcagga 
      gaatttcaa tatttttga ttttgaact gaccgctga 
      tgatgttaa atatttgaa cacgtttga ctgcaatgg 
      ccggttttg cccaatgac tgcggggct ggggcactc 
      acaccaccc attccaaaa cgatgatta ttaattgtg 
      ttacacttt attgaggtt aataaaaat attgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY