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Gene information
General information
TC004497 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3737139..3752241
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004497
    iBeetle screen iB_00709

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    appendages partially remaining - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva cuticle dorsal not closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    tropharium shape irregular - Number of animals showing the phenotype: 2

    egg chamber degenerated - Number of animals showing the phenotype: 2
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 2
    mature egg not present - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004497 RA
      MTDSSEAEL PPQTNGLSD SMDDEERAK IRPVDIDAD 
      VKEMERRKR VEIIMNSKM FREELERII ETQLREGSG 
      TSGLMQQIS DMMGVNKQA GNVFKNSNC VVPINDIRG 
      IEAMGYSKG EKILRCKLA AVFRLIDLY GWTQGTSTL 
      VTARLNQDE EHFLVNPYG MMFHEITAS SLIKVAMQG 
      NVIEQGTTN FSVNISAFS LHAAVHAAR PDIKCIINV 
      HTPSVVAIS ALKHGILPL SQESVVIGD VSTHTYLGG 
      LLDPEEKDK LSRNLGPNN KVLLLSNQG ALCCGETIE 
      EAFFNARNT VLACESQLK LIPVGLDNL VLLNDEIRK 
      KIYDAAHKP PESSPRPDQ PSILEGKVE RKWRVGGIE 
      FEALMRMLD NAGFRTGYI YRHPLVKGD VPKPKNDVE 
      VPPAVSSLG YLLEEEELY RQGLWKKLE GGRKGHDRS 
      RWLNSPNVY QKVEILETG TPDPKKITK WVAEGSPTH 
      SSTPVKIDS ALQFVPKGT NPKEFKLKQ QQIKDYRRA 
      DKISAGPQS HILEGVTWE EARKMQDAT VTQTGDQVV 
      LMGAASKGI IQRGHQHNA MVYKSPYAK NPFDSVTDE 
      ELDEYKKVV EKKVKGEYD TDFSESEGL SSAQINKRM 
      SDHFRSPTS VTSETEEES RDEPQVLRI ETKQAPKPS 
      QPEVVLSDV ETSPTERFL RAERVSVNS HQSVKKGKR 
      LRVNPSLPY YRTNNFAGL PKGSYSIPN PQHFHIVRH 
      NNIPKPLRP LLVRVAPQT RATHYVFNR FNINCDRNN 
      QTVTINIGN YRAVSLAMS MQTVDDFIR PFANALNIL 
      NSTNCTTRE AQTSYVVQD NSNDSSFEK QILQNLQAN 
      LINDLTKSD SDSSECESE SSESDKHNL PNSNLDLRE 
      SESESPEIV EFTEDHSVV INEHLADFS INQFDDSKQ 
      IQKSNLIID LTKSDSNDS SKSESASPE VNDFPNSSF 
      DEGLGDSTK LSPPTQKAP PDDRVVKLG QMIFEYMSE 
      QGFEDSDLV IFPELNHDV NTTKLISKL VETYGWLYG 
      DGLTSEERS DLRIVISSY VLNLIEKDD RMVSSTDSL 
      EFPMVSDKI FSISETLSS IMDQFFASI DTHFNGNLH 
      HSTPEPIKN HYEVVEEVT AFKQDKLDK PGFESYWFS 
      IGSPREQKI REPTPKKII NLDDRPLPP PQNFDFNGS 
      ETLSPIPEK VNRSLFNSP REQKIREPT PKKIINLDD 
      RPLPPPQNF DFNGSETLS PIPEKVNRS LFNSPREQK 
      LHEPTPKKI NVDDYSLPL AQNLDFNCS KTLSPIPEE 
      GSRNLFNDF LDENENIDI LAGGDSFLS NSYVAFDQP 
      ETNIVVCSN VSFHRTVTT VSSAYVKTE NVSFRKSNS 
      FNDDSFYSV EDEGDWMGF EKAKF
    CDS sequence
    • >TC004497 RA
      atgaccgac agcagcgag gcggaattg ccgccccag 
      accaacggt ctttccgat agcatggac gacgaagaa 
      cgcgctaaa attcgtcct gtggacatc gacgccgac 
      gtgaaggag atggagcgt cgcaaacgc gtcgaaata 
      ataatgaac agcaaaatg ttccgcgaa gaactcgaa 
      cgaatcatc gaaacacaa ctacgcgaa ggctccggc 
      acctccggc ctcatgcag caaatctcg gacatgatg 
      ggagtgaac aaacaagcc ggaaacgtg ttcaagaat 
      tcgaactgt gtggttccg atcaacgac attcgcggc 
      atcgaagcc atgggctac agcaaaggc gagaagatc 
      ctacggtgt aaattggcg gctgttttc cgactgatt 
      gacttgtac ggctggacc caaggcacc agcaccttg 
      gtcacggcg cgcttgaac caggacgag gagcacttc 
      ctggtgaac ccttacggg atgatgttc cacgaaatc 
      accgcttcc agtttgatt aaagtggcg atgcagggc 
      aacgtgatc gaacaaggc accacgaac ttttccgtg 
      aatatttcc gccttttcg ctgcatgcc gccgttcat 
      gccgcacgc cccgacatc aaatgcatc atcaacgtc 
      cacacgccg tcagtggtg gccatttcc gccctcaaa 
      cacgggatc ttaccgctg agtcaagaa tcggtggtt 
      attggcgac gtcagtacg cacacgtac ctcggcggt 
      ttgctcgat cccgaggag aaggataaa ttatcgagg 
      aatttagga ccgaataat aaggttctt ttgttgtcg 
      aatcaaggg gcactctgt tgcggagag acaatagaa 
      gaggcgttt ttcaatgcg cgaaatacc gtcctagcg 
      tgcgagtcc cagctcaag ttgattcca gtcggactc 
      gataatttg gtgcttttg aatgatgag atcaggaaa 
      aagatttat gatgcggcg cataaaccg ccagagagt 
      agtccaagg ccggatcaa ccctccatc ttagaaggg 
      aaagtcgag agaaagtgg agggttggt gggattgag 
      tttgaggcc ttgatgcga atgttggat aatgcggga 
      ttcaggacg gggtatatt tatagacat cccctggtc 
      aagggggat gtaccgaag ccgaagaac gacgttgaa 
      gtaccccca gcagtttcc tcccttgga tatttgctc 
      gaggaagag gagctttac agacaagga ctttggaag 
      aagttggaa ggtgggagg aaaggacac gaccgcagc 
      cgctggttg aattcgccg aacgtttat caaaaagtc 
      gaaatttta gaaaccggc acacctgat cctaaaaag 
      attacaaag tgggttgcg gaaggatca ccaactcat 
      tccagtaca cctgtgaaa atcgattcg gccttgcag 
      ttcgtccct aaaggtaca aacccgaaa gaattcaaa 
      ttgaagcaa caacagatc aaggattac cgaagagcc 
      gataaaata tcagccggt ccccagtca cacattctc 
      gaaggtgtg acatgggag gaagctcgc aaaatgcaa 
      gatgctacc gttacccaa acgggagat caagtcgtc 
      ctaatgggg gctgcttcc aaagggatt attcaacgt 
      ggacaccaa cacaacgca atggtgtac aaatccccc 
      tacgccaaa aacccattc gactcggtc accgacgag 
      gagctggac gagtataag aaagtcgtc gagaaaaaa 
      gtcaaaggc gaatatgat acagatttt agcgagtct 
      gaaggttta tcttcggct caaataaac aaaagaatg 
      agtgatcac ttcaggtca ccgacttcc gttactagc 
      gaaactgag gaggagagt agagatgaa ccacaagtt 
      cttcggata gaaaccaaa caagcccca aaacctagc 
      cagcccgaa gttgttttg agtgatgtt gaaacctca 
      ccaaccgaa cgtttccta agagccgag cgagtttca 
      gtcaacagc catcaaagt gttaaaaaa gggaaacgt 
      ctacgtgtt aatccaagc ttgccgtac tatcgcacc 
      aacaatttc gctgggtta cctaaaggc tcatattcc 
      atacctaat cctcaacac ttccatatt gtgcgccac 
      aataacata cctaagcct ttgcgaccc cttcttgta 
      agagttgcc ccacaaacg cgtgccact cattacgtt 
      ttcaatcgg tttaacatt aattgcgat cgcaataat 
      caaactgtt acgattaat attggcaat tatcgggca 
      gtctccctc gccatgtct atgcaaacg gttgatgat 
      tttatcaga ccgtttgct aacgctttg aatatttta 
      aattcgacg aattgtacg acccgagaa gcccaaact 
      tcgtacgtt gtgcaggac aattcaaac gattcgagt 
      ttcgaaaaa caaatcctg caaaacttg caggcgaat 
      ctcatcaat gacctgacg aaaagcgat tcagactcg 
      agtgaatgt gaatcagaa tcatccgaa agtgataaa 
      cacaacttg ccaaactcc aatcttgat ttacgtgaa 
      agtgaatcc gaatcacct gaaatcgtc gaattcact 
      gaggatcat tcggtggtc attaatgaa catttggcc 
      gatttttca atcaatcaa tttgatgac agtaaacag 
      atacaaaaa tcgaatctc attattgac ttgacgaaa 
      agcgattcg aatgattcg agtaaaagt gaatcagcg 
      tcacccgaa gttaacgat tttccaaac agttctttc 
      gatgagggc ttgggtgat tcgaccaag ttgtcgcca 
      ccgacccag aaagcgcca cctgacgac cgagttgtg 
      aaacttggc caaatgatt ttcgagtat atgtccgag 
      caagggttt gaggactcg gatcttgtg attttcccc 
      gaattgaac catgatgtt aatacaacc aaattaata 
      tcaaaattg gttgaaacg tacggatgg ttgtacggt 
      gatggtttg acgagcgaa gaacgcagc gatttgcga 
      attgtgatt tcttcgtac gttttaaac ttgattgag 
      aaagacgac cgaatggtt tcgtcgacc gattcgctc 
      gaatttcca atggtttcg gacaagatt ttcagcatt 
      tctgaaaca ctctcgagt atcatggac cagtttttc 
      gcctcaatt gatactcac tttaacggt aatctgcac 
      cattcgacc cccgaacca atcaaaaac cactacgaa 
      gtggttgaa gaagtgacc gcgttcaaa caagacaag 
      ctggataag cccgggttt gagtcttac tggttttcc 
      attggttcc ccgagggaa caaaagata cgcgagccg 
      actccgaag aagattatc aatcttgat gatagaccc 
      ttaccccca ccccaaaat tttgatttt aatggctct 
      gaaaccttg tcaccgatc ccggagaaa gtgaaccgc 
      agtttattt aattctcca agggagcaa aagatacgc 
      gagccaact ccgaagaag attatcaat cttgatgat 
      agaccctta cccccaccc caaaatttt gattttaac 
      ggctctgaa accttgtca ccaatccca gagaaagtg 
      aaccgcagt ttatttaat tctccaagg gagcaaaag 
      ttacacgaa ccaactccc aagaaaatt aatgttgat 
      gattattcc ttacctctg gcacaaaat cttgatttt 
      aactgctct aaaaccttg tcgccgatc ccggaggaa 
      ggcagtcgc aacttattc aatgatttt ttggacgaa 
      aacgaaaat attgatatc ttggctggg ggtgattca 
      tttttgtcc aattcgtac gttgctttc gatcaaccc 
      gagactaac atagttgtg tgttcgaat gtctcgttc 
      catcgcact gttacaaca gtaagttcg gcctacgtc 
      aagacggag aatgttagt tttaggaaa agtaattcc 
      ttcaacgat gatagtttt tacagcgtt gaggacgag 
      ggggattgg atgggtttt gaaaaggcc aagttttaa
    mRNA sequence
    • >TC004497 RA
      ggtggaaac tgcagaggg tacgcgaaa cgaatttct 
      aatttttag tgaccgaca ttgtaatcg tgagtgctc 
      tcagtcggt atttttgaa gtgaagttt gcgattcga 
      agcttgttg aggcttcgc agggccccc ggcgaccgt 
      tgaatcgat ggggcgtat gctcgcctt gtgtcatta 
      gtgcataga gaaacatta ccgtcagtt gagtgattt 
      aaataacgc cggtcttta accacattc gaggaagca 
      acgataaaa aatgaccga cagcagcga ggcggaatt 
      gccgcccca gaccaacgg tctttccga tagcatgga 
      cgacgaaga acgcgctaa aattcgtcc tgtggacat 
      cgacgccga cgtgaagga gatggagcg tcgcaaacg 
      cgtcgaaat aataatgaa cagcaaaat gttccgcga 
      agaactcga acgaatcat cgaaacaca actacgcga 
      aggctccgg cacctccgg cctcatgca gcaaatctc 
      ggacatgat gggagtgaa caaacaagc cggaaacgt 
      gttcaagaa ttcgaactg tgtggttcc gatcaacga 
      cattcgcgg catcgaagc catgggcta cagcaaagg 
      cgagaagat cctacggtg taaattggc ggctgtttt 
      ccgactgat tgacttgta cggctggac ccaaggcac 
      cagcacctt ggtcacggc gcgcttgaa ccaggacga 
      ggagcactt cctggtgaa cccttacgg gatgatgtt 
      ccacgaaat caccgcttc cagtttgat taaagtggc 
      gatgcaggg caacgtgat cgaacaagg caccacgaa 
      cttttccgt gaatatttc cgccttttc gctgcatgc 
      cgccgttca tgccgcacg ccccgacat caaatgcat 
      catcaacgt ccacacgcc gtcagtggt ggccatttc 
      cgccctcaa acacgggat cttaccgct gagtcaaga 
      atcggtggt tattggcga cgtcagtac gcacacgta 
      cctcggcgg tttgctcga tcccgagga gaaggataa 
      attatcgag gaatttagg accgaataa taaggttct 
      tttgttgtc gaatcaagg ggcactctg ttgcggaga 
      gacaataga agaggcgtt tttcaatgc gcgaaatac 
      cgtcctagc gtgcgagtc ccagctcaa gttgattcc 
      agtcggact cgataattt ggtgctttt gaatgatga 
      gatcaggaa aaagattta tgatgcggc gcataaacc 
      gccagagag tagtccaag gccggatca accctccat 
      cttagaagg gaaagtcga gagaaagtg gagggttgg 
      tgggattga gtttgaggc cttgatgcg aatgttgga 
      taatgcggg attcaggac ggggtatat ttatagaca 
      tcccctggt caaggggga tgtaccgaa gccgaagaa 
      cgacgttga agtaccccc agcagtttc ctcccttgg 
      atatttgct cgaggaaga ggagcttta cagacaagg 
      actttggaa gaagttgga aggtgggag gaaaggaca 
      cgaccgcag ccgctggtt gaattcgcc gaacgttta 
      tcaaaaagt cgaaatttt agaaaccgg cacacctga 
      tcctaaaaa gattacaaa gtgggttgc ggaaggatc 
      accaactca ttccagtac acctgtgaa aatcgattc 
      ggccttgca gttcgtccc taaaggtac aaacccgaa 
      agaattcaa attgaagca acaacagat caaggatta 
      ccgaagagc cgataaaat atcagccgg tccccagtc 
      acacattct cgaaggtgt gacatggga ggaagctcg 
      caaaatgca agatgctac cgttaccca aacgggaga 
      tcaagtcgt cctaatggg ggctgcttc caaagggat 
      tattcaacg tggacacca acacaacgc aatggtgta 
      caaatcccc ctacgccaa aaacccatt cgactcggt 
      caccgacga ggagctgga cgagtataa gaaagtcgt 
      cgagaaaaa agtcaaagg cgaatatga tacagattt 
      tagcgagtc tgaaggttt atcttcggc tcaaataaa 
      caaaagaat gagtgatca cttcaggtc accgacttc 
      cgttactag cgaaactga ggaggagag tagagatga 
      accacaagt tcttcggat agaaaccaa acaagcccc 
      aaaacctag ccagcccga agttgtttt gagtgatgt 
      tgaaacctc accaaccga acgtttcct aagagccga 
      gcgagtttc agtcaacag ccatcaaag tgttaaaaa 
      agggaaacg tctacgtgt taatccaag cttgccgta 
      ctatcgcac caacaattt cgctgggtt acctaaagg 
      ctcatattc catacctaa tcctcaaca cttccatat 
      tgtgcgcca caataacat acctaagcc tttgcgacc 
      ccttcttgt aagagttgc cccacaaac gcgtgccac 
      tcattacgt tttcaatcg gtttaacat taattgcga 
      tcgcaataa tcaaactgt tacgattaa tattggcaa 
      ttatcgggc agtctccct cgccatgtc tatgcaaac 
      ggttgatga ttttatcag accgtttgc taacgcttt 
      gaatatttt aaattcgac gaattgtac gacccgaga 
      agcccaaac ttcgtacgt tgtgcagga caattcaaa 
      cgattcgag tttcgaaaa acaaatcct gcaaaactt 
      gcaggcgaa tctcatcaa tgacctgac gaaaagcga 
      ttcagactc gagtgaatg tgaatcaga atcatccga 
      aagtgataa acacaactt gccaaactc caatcttga 
      tttacgtga aagtgaatc cgaatcacc tgaaatcgt 
      cgaattcac tgaggatca ttcggtggt cattaatga 
      acatttggc cgatttttc aatcaatca atttgatga 
      cagtaaaca gatacaaaa atcgaatct cattattga 
      cttgacgaa aagcgattc gaatgattc gagtaaaag 
      tgaatcagc gtcacccga agttaacga ttttccaaa 
      cagttcttt cgatgaggg cttgggtga ttcgaccaa 
      gttgtcgcc accgaccca gaaagcgcc acctgacga 
      ccgagttgt gaaacttgg ccaaatgat tttcgagta 
      tatgtccga gcaagggtt tgaggactc ggatcttgt 
      gattttccc cgaattgaa ccatgatgt taatacaac 
      caaattaat atcaaaatt ggttgaaac gtacggatg 
      gttgtacgg tgatggttt gacgagcga agaacgcag 
      cgatttgcg aattgtgat ttcttcgta cgttttaaa 
      cttgattga gaaagacga ccgaatggt ttcgtcgac 
      cgattcgct cgaatttcc aatggtttc ggacaagat 
      tttcagcat ttctgaaac actctcgag tatcatgga 
      ccagttttt cgcctcaat tgatactca ctttaacgg 
      taatctgca ccattcgac ccccgaacc aatcaaaaa 
      ccactacga agtggttga agaagtgac cgcgttcaa 
      acaagacaa gctggataa gcccgggtt tgagtctta 
      ctggttttc cattggttc cccgaggga acaaaagat 
      acgcgagcc gactccgaa gaagattat caatcttga 
      tgatagacc cttaccccc accccaaaa ttttgattt 
      taatggctc tgaaacctt gtcaccgat cccggagaa 
      agtgaaccg cagtttatt taattctcc aagggagca 
      aaagatacg cgagccaac tccgaagaa gattatcaa 
      tcttgatga tagaccctt acccccacc ccaaaattt 
      tgattttaa cggctctga aaccttgtc accaatccc 
      agagaaagt gaaccgcag tttatttaa ttctccaag 
      ggagcaaaa gttacacga accaactcc caagaaaat 
      taatgttga tgattattc cttacctct ggcacaaaa 
      tcttgattt taactgctc taaaacctt gtcgccgat 
      cccggagga aggcagtcg caacttatt caatgattt 
      tttggacga aaacgaaaa tattgatat cttggctgg 
      gggtgattc atttttgtc caattcgta cgttgcttt 
      cgatcaacc cgagactaa catagttgt gtgttcgaa 
      tgtctcgtt ccatcgcac tgttacaac agtaagttc 
      ggcctacgt caagacgga gaatgttag ttttaggaa 
      aagtaattc cttcaacga tgatagttt ttacagcgt 
      tgaggacga gggggattg gatgggttt tgaaaaggc 
      caagtttta ataattttt gcttttttt tatatgttt 
      tgtatgcaa ataaatgtt tcattgcat g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY