Status
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Gene information
General information
TC032012
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004490,TC004491
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3803546..3808021
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032012
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00707

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection



    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032012 RA
      MAPHSCKLY LSDIIATMA EDNRKVSME VNGVSENGF 
      TDAPSMPWF GMDIGGSLC KLVYFEPKD ITKDEADSE 
      VETLRNIRR YLTKNSAYG KTGHRDTHL QMDDVYIRG 
      RRGTLHFIR FPSSEMVNF LALAKSKGM ANLVTTVCA 
      TGGGAFKFE EDFRREVNM KLEKFDEFD SLLKGLQFA 
      DAQNPSECY YWANPTDDA CCSKVKYDF SNPYPFLIV 
      NIGSGVSVL AVYSATDYK RISGTSLGG GTFLGLCCL 
      LTGCNTFEE AIQLAAEGD NTRVDKLVR DIYGGDYER 
      FGLPGDLVA SSFGQMNSR EKRSKVSRQ DLARATLVT 
      ITNNIGSIA RMCAIQEKI DRVVFMGNF LRVNPIAMK 
      LLAYAMNYW SKGTMKALF LEHEGYFGA IGCLLQFHE 
      AKR
    • >TC032012 RB
      MAPHSCKLY LSDIIATMA EDNRKVSME VNGVSENGF 
      TDAPSMPWF GMDIGGSLC KLVYFEPKD ITKDEADSE 
      VETLRNIRR YLTKNSAYG KTGHRDTHL QMDDVYIRG 
      RRGTLHFIR FPSSEMVNF LALAKSKGM ANLVTTVCA 
      TGGGAFKFE EDFRREVNM KLEKFDEFD SLLKGLQFA 
      DAQNPSECY YWANPTDDA CCSKVKYDF SNPYPFLIV 
      NIGSGVSVL AVYSATDYK RISGTSLGG GTFLGLCCL 
      LTGCNTFEE AIQLAAEGD NTRVDKLVR DIYGGDYER 
      FGLPGDLVA SSFSGFSFG QMNSREKRS KVSRQDLAR 
      ATLVTITNN IGSIARMCA IQEKIDRVV FMGNFLRVN 
      PIAMKLLAY AMNYWSKGT MKALFLEHE GYFGAIGCL 
      LQFHEAKR
    CDS sequence
    • >TC032012 RA
      atggcccct cacagctgt aaactttac ttaagtgat 
      attatcgcg acaatggcc gaagacaat agaaaagtc 
      tcaatggaa gttaatggg gtgagcgaa aacggcttc 
      acagacgct ccctcaatg ccgtggttt ggaatggac 
      attggcggc agtttatgc aagttggtc tatttcgaa 
      ccgaaagac atcaccaaa gacgaggcc gattccgaa 
      gtggaaacc ctacgcaac atccggaga tatctaaca 
      aaaaactca gcatatggg aaaaccggc catcgagac 
      acccactta cagatggac gatgtttac attcggggc 
      aggagaggc actttgcac tttattcgg tttccgtcg 
      agcgaaatg gtcaacttt ttggctttg gccaagtcc 
      aaaggcatg gccaacttg gttacgacg gtttgtgcg 
      acgggaggg ggcgctttc aagtttgag gaggacttc 
      agaagggaa gttaacatg aagttggaa aaatttgac 
      gagttcgat tcgcttttg aagggcttg cagttcgca 
      gacgcccag aacccgtcc gagtgttac tattgggcg 
      aatccgacc gacgatgct tgctgttct aaagtcaaa 
      tatgacttc tcgaatcca tacccgttt ctgattgtc 
      aatatcggg tcaggtgtt agcgttttg gccgtctat 
      tcggccacc gattacaag aggatctca gggacaagt 
      ttgggcgga ggcactttt ttgggcctt tgctgtttg 
      ttgactggt tgcaatacg ttcgaagag gcgatacag 
      ttggctgca gaaggtgat aacacacga gttgataag 
      ctggtgcgt gatatatac ggtggtgac tacgaacgg 
      tttgggtta ccgggagat ttggtcgct agcagcttt 
      gggcaaatg aactctcgc gagaagcgc agcaaagtc 
      agtcgacag gatctagcg agggctacg ttagtgacc 
      atcactaac aacatcggg tctattgct agaatgtgc 
      gctattcag gagaaaatt gacagggtc gtttttatg 
      ggcaatttc ctacgagta aaccccatc gccatgaaa 
      ctgctcgca tacgccatg aactactgg tccaaggga 
      acaatgaaa gccctattt ctggaacac gaaggctac 
      tttggtgct attggttgt ctccttcaa tttcacgag 
      gccaaaaga tga
    • >TC032012 RB
      atggcccct cacagctgt aaactttac ttaagtgat 
      attatcgcg acaatggcc gaagacaat agaaaagtc 
      tcaatggaa gttaatggg gtgagcgaa aacggcttc 
      acagacgct ccctcaatg ccgtggttt ggaatggac 
      attggcggc agtttatgc aagttggtc tatttcgaa 
      ccgaaagac atcaccaaa gacgaggcc gattccgaa 
      gtggaaacc ctacgcaac atccggaga tatctaaca 
      aaaaactca gcatatggg aaaaccggc catcgagac 
      acccactta cagatggac gatgtttac attcggggc 
      aggagaggc actttgcac tttattcgg tttccgtcg 
      agcgaaatg gtcaacttt ttggctttg gccaagtcc 
      aaaggcatg gccaacttg gttacgacg gtttgtgcg 
      acgggaggg ggcgctttc aagtttgag gaggacttc 
      agaagggaa gttaacatg aagttggaa aaatttgac 
      gagttcgat tcgcttttg aagggcttg cagttcgca 
      gacgcccag aacccgtcc gagtgttac tattgggcg 
      aatccgacc gacgatgct tgctgttct aaagtcaaa 
      tatgacttc tcgaatcca tacccgttt ctgattgtc 
      aatatcggg tcaggtgtt agcgttttg gccgtctat 
      tcggccacc gattacaag aggatctca gggacaagt 
      ttgggcgga ggcactttt ttgggcctt tgctgtttg 
      ttgactggt tgcaatacg ttcgaagag gcgatacag 
      ttggctgca gaaggtgat aacacacga gttgataag 
      ctggtgcgt gatatatac ggtggtgac tacgaacgg 
      tttgggtta ccgggagat ttggtcgct agcagtttc 
      tcgggcttt agctttggg caaatgaac tctcgcgag 
      aagcgcagc aaagtcagt cgacaggat ctagcgagg 
      gctacgtta gtgaccatc actaacaac atcgggtct 
      attgctaga atgtgcgct attcaggag aaaattgac 
      agggtcgtt tttatgggc aatttccta cgagtaaac 
      cccatcgcc atgaaactg ctcgcatac gccatgaac 
      tactggtcc aagggaaca atgaaagcc ctatttctg 
      gaacacgaa ggctacttt ggtgctatt ggttgtctc 
      cttcaattt cacgaggcc aaaagatga
    mRNA sequence
    • >TC032012 RA
      ttaaatagg ccgggttat cactttgta gtttattaa 
      aatttgtta taattgtgt ttttgaggt cgttgttaa 
      agtagattt ctgttgtga aacgtcaca ttttgcaaa 
      cgtcaaatt gtatgtcat gacttggcg tgtgggttt 
      tcgcacttt atttcgcgt ttttattca tgtaagaaa 
      taattagta ccgtgcctt ctttttcta aatgacacg 
      taatgatct agttgtgaa acaccagca aaacacata 
      tttcaccgt cgagtgtag tgtcatttt actttgctt 
      caaaatcgg ccaaaacga tacaaaaat gatggaaga 
      ggtcgtaca ttacaatgg cccctcaca gctgtaaac 
      tttacttaa gtgatatta tcgcgacaa tggccgaag 
      acaatagaa aagtctcaa tggaagtta atggggtga 
      gcgaaaacg gcttcacag acgctccct caatgccgt 
      ggtttggaa tggacattg gcggcagtt tatgcaagt 
      tggtctatt tcgaaccga aagacatca ccaaagacg 
      aggccgatt ccgaagtgg aaaccctac gcaacatcc 
      ggagatatc taacaaaaa actcagcat atgggaaaa 
      ccggccatc gagacaccc acttacaga tggacgatg 
      tttacattc ggggcagga gaggcactt tgcacttta 
      ttcggtttc cgtcgagcg aaatggtca actttttgg 
      ctttggcca agtccaaag gcatggcca acttggtta 
      cgacggttt gtgcgacgg gagggggcg ctttcaagt 
      ttgaggagg acttcagaa gggaagtta acatgaagt 
      tggaaaaat ttgacgagt tcgattcgc ttttgaagg 
      gcttgcagt tcgcagacg cccagaacc cgtccgagt 
      gttactatt gggcgaatc cgaccgacg atgcttgct 
      gttctaaag tcaaatatg acttctcga atccatacc 
      cgtttctga ttgtcaata tcgggtcag gtgttagcg 
      ttttggccg tctattcgg ccaccgatt acaagagga 
      tctcaggga caagtttgg gcggaggca cttttttgg 
      gcctttgct gtttgttga ctggttgca atacgttcg 
      aagaggcga tacagttgg ctgcagaag gtgataaca 
      cacgagttg ataagctgg tgcgtgata tatacggtg 
      gtgactacg aacggtttg ggttaccgg gagatttgg 
      tcgctagca gctttgggc aaatgaact ctcgcgaga 
      agcgcagca aagtcagtc gacaggatc tagcgaggg 
      ctacgttag tgaccatca ctaacaaca tcgggtcta 
      ttgctagaa tgtgcgcta ttcaggaga aaattgaca 
      gggtcgttt ttatgggca atttcctac gagtaaacc 
      ccatcgcca tgaaactgc tcgcatacg ccatgaact 
      actggtcca agggaacaa tgaaagccc tatttctgg 
      aacacgaag gctactttg gtgctattg gttgtctcc 
      ttcaatttc acgaggcca aaagatgaa ttataaaca 
      ttccagtga aaggtgttg gagtttagt acaaaataa 
      aacaatgtt ggacatttt tagcaaatg aggttaatt 
      cttggcgtt ttattgtag attttgtac actgctttt 
      ataaaaata gtacgtatg tttgaaggt gtagatttt 
      tttaatgta tggtttttc tacattttt taaataaac 
      ttgataagt gagaacttg gtgtaggct catcggtac 
      gtgaaatga cgcacatgt tttatttca aaggcacat 
      gaaactatt attaaatta tttattgat ag
    • >TC032012 RB
      ttaaatagg ccgggttat cactttgta gtttattaa 
      aatttgtta taattgtgt ttttgaggt cgttgttaa 
      agtagattt ctgttgtga aacgtcaca ttttgcaaa 
      cgtcaaatt gtatgtcat gacttggcg tgtgggttt 
      tcgcacttt atttcgcgt ttttattca tgtaagaaa 
      taattagta ccgtgcctt ctttttcta aatgacacg 
      taatgatct agttgtgaa acaccagca aaacacata 
      tttcaccgt cgagtgtag tgtcatttt actttgctt 
      caaaatcgg ccaaaacga tacaaaaat gatggaaga 
      ggtcgtaca ttacaatgg cccctcaca gctgtaaac 
      tttacttaa gtgatatta tcgcgacaa tggccgaag 
      acaatagaa aagtctcaa tggaagtta atggggtga 
      gcgaaaacg gcttcacag acgctccct caatgccgt 
      ggtttggaa tggacattg gcggcagtt tatgcaagt 
      tggtctatt tcgaaccga aagacatca ccaaagacg 
      aggccgatt ccgaagtgg aaaccctac gcaacatcc 
      ggagatatc taacaaaaa actcagcat atgggaaaa 
      ccggccatc gagacaccc acttacaga tggacgatg 
      tttacattc ggggcagga gaggcactt tgcacttta 
      ttcggtttc cgtcgagcg aaatggtca actttttgg 
      ctttggcca agtccaaag gcatggcca acttggtta 
      cgacggttt gtgcgacgg gagggggcg ctttcaagt 
      ttgaggagg acttcagaa gggaagtta acatgaagt 
      tggaaaaat ttgacgagt tcgattcgc ttttgaagg 
      gcttgcagt tcgcagacg cccagaacc cgtccgagt 
      gttactatt gggcgaatc cgaccgacg atgcttgct 
      gttctaaag tcaaatatg acttctcga atccatacc 
      cgtttctga ttgtcaata tcgggtcag gtgttagcg 
      ttttggccg tctattcgg ccaccgatt acaagagga 
      tctcaggga caagtttgg gcggaggca cttttttgg 
      gcctttgct gtttgttga ctggttgca atacgttcg 
      aagaggcga tacagttgg ctgcagaag gtgataaca 
      cacgagttg ataagctgg tgcgtgata tatacggtg 
      gtgactacg aacggtttg ggttaccgg gagatttgg 
      tcgctagca gtttctcgg gctttagct ttgggcaaa 
      tgaactctc gcgagaagc gcagcaaag tcagtcgac 
      aggatctag cgagggcta cgttagtga ccatcacta 
      acaacatcg ggtctattg ctagaatgt gcgctattc 
      aggagaaaa ttgacaggg tcgttttta tgggcaatt 
      tcctacgag taaacccca tcgccatga aactgctcg 
      catacgcca tgaactact ggtccaagg gaacaatga 
      aagccctat ttctggaac acgaaggct actttggtg 
      ctattggtt gtctccttc aatttcacg aggccaaaa 
      gatgaatta taaacattc cagtgaaag gtgttggag 
      tttagtaca aaataaaac aatgttgga catttttag 
      caaatgagg ttaattctt ggcgtttta ttgtagatt 
      ttgtacact gcttttata aaaatagta cgtatgttt 
      gaaggtgta gattttttt aatgtatgg tttttctac 
      attttttaa ataaacttg ataagtgag aacttggtg 
      taggctcat cggtacgtg aaatgacgc acatgtttt 
      atttcaaag gcacatgaa actattatt aaattattt 
      attgatag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY