Status
Please wait ...
Gene information
General information
TC004438 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4934652..4940337
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004438
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00697

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004438 RA
      MKNVWLVLL LLIYFKPST SLKCNRDDK KCLCLPGAE 
      FEFQCPSSF ENGDTGDYP DYVIHIKED KDILIDCSP 
      NLKQFNITS LPNVTIERT ESFRMRFCP LPPRNFKEV 
      LDWFGIKQV KYLQYSHYE LANTTLTKD YFEGLEDLE 
      KLSLENNGL VDVEEDVFA NTPKLTSLD LQVNKIKLG 
      ANLFNNTPL LKSLDISQN NLDYLPPDL FKKLEDLEL 
      LHVWDNKIR FIDDSTFQG LTNVKSIEL SGNLIETIT 
      ENAFKNLSN LERVNLSMN KLQYVPGDL FRHNQKLKL 
      VLLKMNEGL YLPGYLFSN MSQLEEVDL TDCKLKGIS 
      SDIFKHSLN LKRIKLAHN TLDFIPQTL FEGLTNLEE 
      IYLNNNNIE TIDQIFKTL RQLKILTLE GNRIRDVGK 
      AAFDDLIRL EKIILRHNQ ITKIDNRAF YNNGNLKTI 
      DLAHNQITG NYSSKLVLL ENVHDAETI DLSHNLITR 
      VDDIVTMYN KNKLKSLDL SYNRIEVIG LQELQPFTK 
      KVIIKLDHN HIKTVDFSY VEVVATSGD AKDALSDPD 
      PWHSVIDLN ENPLNCDCH NFDLVRYVT NELRPEIKV 
      MFDLKMDNL KCANPPPLE DFPVESLVP KLLICPVED 
      GCPERCLCS QRPFDRTII INCADKNLT QYPQLNLSD 
      FTNIDFVQT EVHLEGNSL NTGPGKNLT GYGNVTELS 
      LSSNKIETI SWVPPNIKV LKLDNNKIS YLNWEVLHT 
      LNESNLHSL TLHNNPWTC DCQAANLTN FLRQNPKIL 
      DAKHVYCFN STNRLIELN RTDLCHEWQ VVYLVLIVL 
      SVLFFVALA FALYYRYQQ ELKVWLFAH NLCLWWVTE 
      EELDKDKIY DAFISYSHK DEDFVTQNL LPVLEGGPQ 
      PYKLCLHYR NWIPGEFIT TQVTNSVLE SRRTLVVLS 
      PNFLESVWG KMEFRTAHT QAMTEGRAR VIIVLYGDV 
      DVDSLDDEL KTYLKTNTY VKWGDPYFW NKLKYALPH 
      TKRNVANQQ KMANVMNCI DDKFKLVVP VTNSPGSTP 
      PVLALDANM LKHPLNFVP SQEMDTPPA EAGTLLVST 
      GL
    CDS sequence
    • >TC004438 RA
      atgaaaaat gtgtggcta gttcttctt ttactcatc 
      tatttcaaa ccctcaaca agcctcaaa tgcaaccgc 
      gacgacaaa aagtgtctc tgcctcccg ggggctgaa 
      ttcgaattt cagtgcccc tctagtttt gaaaatggg 
      gatactggg gattatccc gactacgtt attcatata 
      aaagaggac aaggatatt ttgatagac tgttctcca 
      aatttgaag caatttaat atcacttcg cttcctaat 
      gttaccatt gaaaggact gaaagcttt cgaatgagg 
      ttttgtccg ttacctccc cgaaacttc aaagaggtt 
      ttggactgg tttgggatt aaacaagtc aaatatttg 
      caatactca cattacgaa ttggctaat acgacactg 
      actaaagac tattttgag ggtctggaa gatctggaa 
      aaattatca ttggagaat aatggacta gtcgatgtg 
      gaagaggac gtttttgca aatactcca aagttgaca 
      tcactcgac ttacaagtc aataaaatc aaacttggg 
      gcgaattta ttcaacaac acaccactg ttgaaatcg 
      ttggatatt tcccaaaac aacttggac taccttccg 
      ccagattta tttaaaaaa ttggaagac ttggagttg 
      ttacatgtt tgggataat aaaattcgg ttcatcgat 
      gattcaact ttccaaggt ttgacgaac gtaaaatcg 
      atcgaattg agtggaaat ctgattgaa acgatcaca 
      gagaatgct ttcaaaaat ttaagcaac ctggaacgt 
      gttaattta tcgatgaat aaactgcaa tatgtaccg 
      ggcgatctc tttaggcac aatcaaaaa ctaaaatta 
      gttttactg aaaatgaat gaagggtta taccttccg 
      ggttactta ttttcgaat atgtctcag cttgaggaa 
      gttgacttg actgattgt aaactgaaa ggaatttca 
      tccgatatt ttcaaacat tccctaaac ttgaaacga 
      atcaaactg gcccacaac acgctggac tttataccg 
      caaactctt ttcgaaggc ctgacaaat ctggaagag 
      atttatttg aacaacaac aacattgag acaattgac 
      caaattttc aagacactt agacaactc aaaatcctg 
      acgcttgaa ggaaaccgg atcagggac gttggaaaa 
      gccgcattt gacgacttg atacgtttg gagaagatt 
      attttgcgc cacaatcaa attacaaag atcgacaac 
      agagcattt tacaacaat ggtaatttg aaaacgatc 
      gatttggcc cataaccag attacgggc aactacagt 
      tcaaaacta gtccttttg gaaaacgtc cacgacgct 
      gaaactatt gacttaagt cacaactta ataacgcga 
      gtcgatgac attgtcacg atgtacaac aaaaacaag 
      ttaaagtct ctggatttg agttacaac cgcatcgaa 
      gtcataggc ttacaagag ctgcaaccg ttcactaaa 
      aaagttatc atcaagctg gaccataac cacatcaag 
      acggttgat tttagttac gttgaagtt gttgccaca 
      agtggagac gccaaagac gctttatct gatcccgat 
      ccgtggcat tcagtcatc gacttgaat gagaatcct 
      ttgaattgt gactgccac aattttgat ttagtgaga 
      tatgtcaca aacgagcta aggccggaa attaaagta 
      atgtttgac ttgaaaatg gacaactta aaatgtgca 
      aacccaccc ccacttgag gactttccg gttgagtca 
      ttggtaccg aaattgctc atttgtcca gttgaggat 
      gggtgtccc gagaggtgt ttgtgtagt caaagaccc 
      tttgaccga acgattata ataaattgt gcggataaa 
      aatctgaca cagtatcct caactcaat ttgagtgat 
      tttacaaac attgatttt gttcaaact gaagtgcat 
      cttgagggt aattcttta aatactggg cctggtaaa 
      aatttgact ggttatggt aatgtaacg gagcttagt 
      ctctcgtcc aataagatt gaaacgatt tcctgggtg 
      cctccaaac attaaggtt ttgaaattg gacaataac 
      aagatcagt tatttaaac tgggaggtt ttgcacacg 
      ttgaatgag tcaaattta cacagtctt accttgcat 
      aataacccg tggacttgc gactgccag gcggcaaat 
      ttgactaat tttctccgc caaaaccca aaaattttg 
      gacgcaaaa catgtttat tgtttcaac agcacgaac 
      cgtttgatt gagttgaac cggaccgac ctttgtcac 
      gaatggcaa gtcgtttat ctcgtcctt atagtcctg 
      tcagtgctc ttcttcgtc gcccttgcc tttgccctc 
      tattaccgc taccagcag gagctaaaa gtctggctc 
      tttgcgcac aacttgtgc ctgtggtgg gtcacagaa 
      gaggagctc gacaaggac aaaatctac gacgccttc 
      atcagctac tcgcacaag gacgaagac ttcgtcacg 
      caaaacctc ctcccggtc cttgaaggc ggcccccaa 
      ccctacaaa ctctgtctc cactaccgc aattggatc 
      ccaggcgaa tttatcacg actcaagtc acaaactcg 
      gttctcgaa tcgcgccga acgcttgtg gtgctttcg 
      cccaacttc ctggaaagt gtttggggg aaaatggag 
      ttccggacc gctcacacg caagccatg accgaagga 
      cgggctcgt gttatcatt gtcttgtac ggagacgtc 
      gatgtggat agtttggac gacgaactc aaaacgtac 
      ttgaaaaca aacacttat gtgaagtgg ggcgatccc 
      tacttctgg aacaagttg aaatatgct ttgccgcac 
      actaaacga aacgtcgca aatcagcaa aaaatggca 
      aatgttatg aactgtatc gacgataag tttaagtta 
      gttgtgccc gttacgaat agtccaggt agtactccg 
      ccagttctg gcacttgat gcaaatatg ttgaaacat 
      ccactcaac ttcgtgcct agtcaagaa atggatact 
      cctcctgcc gaagctggg actttgctg gtttcgact 
      ggtctctaa
    mRNA sequence
    • >TC004438 RA
      tgagttcga cttgcgagc taaatgctc ggttttttg 
      gaggggttt tggtttacg tcacaagca tgtcgtaga 
      ataaatgtc atcatcgtt gtttctatt ggtttggct 
      ttcccacaa acagtaccc gagtgagtg tgggtagca 
      ggtggtttt gtaaaattt tgatttttg ggcagttct 
      tcctcttgg tgcttacaa atagtgatc aaatttgtt 
      tggagaagt ttgtgatgt tacacagtt tgaattgtg 
      cgtagtgca aatcgataa gattgactg tttttgcaa 
      tatggagtt ttgttgatt tctcggaaa tgtgatttt 
      gtgaaatcg acttgttta ttggaagag taccgtctt 
      gacattcta atggttttc aaactataa acatgaaaa 
      atgtgtggc tagttcttc ttttactca tctatttca 
      aaccctcaa caagcctca aatgcaacc gcgacgaca 
      aaaagtgtc tctgcctcc cgggggctg aattcgaat 
      ttcagtgcc cctctagtt ttgaaaatg gggatactg 
      gggattatc ccgactacg ttattcata taaaagagg 
      acaaggata ttttgatag actgttctc caaatttga 
      agcaattta atatcactt cgcttccta atgttacca 
      ttgaaagga ctgaaagct ttcgaatga ggttttgtc 
      cgttacctc cccgaaact tcaaagagg ttttggact 
      ggtttggga ttaaacaag tcaaatatt tgcaatact 
      cacattacg aattggcta atacgacac tgactaaag 
      actattttg agggtctgg aagatctgg aaaaattat 
      cattggaga ataatggac tagtcgatg tggaagagg 
      acgtttttg caaatactc caaagttga catcactcg 
      acttacaag tcaataaaa tcaaacttg gggcgaatt 
      tattcaaca acacaccac tgttgaaat cgttggata 
      tttcccaaa acaacttgg actaccttc cgccagatt 
      tatttaaaa aattggaag acttggagt tgttacatg 
      tttgggata ataaaattc ggttcatcg atgattcaa 
      ctttccaag gtttgacga acgtaaaat cgatcgaat 
      tgagtggaa atctgattg aaacgatca cagagaatg 
      ctttcaaaa atttaagca acctggaac gtgttaatt 
      tatcgatga ataaactgc aatatgtac cgggcgatc 
      tctttaggc acaatcaaa aactaaaat tagttttac 
      tgaaaatga atgaagggt tataccttc cgggttact 
      tattttcga atatgtctc agcttgagg aagttgact 
      tgactgatt gtaaactga aaggaattt catccgata 
      ttttcaaac attccctaa acttgaaac gaatcaaac 
      tggcccaca acacgctgg actttatac cgcaaactc 
      ttttcgaag gcctgacaa atctggaag agatttatt 
      tgaacaaca acaacattg agacaattg accaaattt 
      tcaagacac ttagacaac tcaaaatcc tgacgcttg 
      aaggaaacc ggatcaggg acgttggaa aagccgcat 
      ttgacgact tgatacgtt tggagaaga ttattttgc 
      gccacaatc aaattacaa agatcgaca acagagcat 
      tttacaaca atggtaatt tgaaaacga tcgatttgg 
      cccataacc agattacgg gcaactaca gttcaaaac 
      tagtccttt tggaaaacg tccacgacg ctgaaacta 
      ttgacttaa gtcacaact taataacgc gagtcgatg 
      acattgtca cgatgtaca acaaaaaca agttaaagt 
      ctctggatt tgagttaca accgcatcg aagtcatag 
      gcttacaag agctgcaac cgttcacta aaaaagtta 
      tcatcaagc tggaccata accacatca agacggttg 
      attttagtt acgttgaag ttgttgcca caagtggag 
      acgccaaag acgctttat ctgatcccg atccgtggc 
      attcagtca tcgacttga atgagaatc ctttgaatt 
      gtgactgcc acaattttg atttagtga gatatgtca 
      caaacgagc taaggccgg aaattaaag taatgtttg 
      acttgaaaa tggacaact taaaatgtg caaacccac 
      ccccacttg aggactttc cggttgagt cattggtac 
      cgaaattgc tcatttgtc cagttgagg atgggtgtc 
      ccgagaggt gtttgtgta gtcaaagac cctttgacc 
      gaacgatta taataaatt gtgcggata aaaatctga 
      cacagtatc ctcaactca atttgagtg attttacaa 
      acattgatt ttgttcaaa ctgaagtgc atcttgagg 
      gtaattctt taaatactg ggcctggta aaaatttga 
      ctggttatg gtaatgtaa cggagctta gtctctcgt 
      ccaataaga ttgaaacga tttcctggg tgcctccaa 
      acattaagg ttttgaaat tggacaata acaagatca 
      gttatttaa actgggagg ttttgcaca cgttgaatg 
      agtcaaatt tacacagtc ttaccttgc ataataacc 
      cgtggactt gcgactgcc aggcggcaa atttgacta 
      attttctcc gccaaaacc caaaaattt tggacgcaa 
      aacatgttt attgtttca acagcacga accgtttga 
      ttgagttga accggaccg acctttgtc acgaatggc 
      aagtcgttt atctcgtcc ttatagtcc tgtcagtgc 
      tcttcttcg tcgcccttg cctttgccc tctattacc 
      gctaccagc aggagctaa aagtctggc tctttgcgc 
      acaacttgt gcctgtggt gggtcacag aagaggagc 
      tcgacaagg acaaaatct acgacgcct tcatcagct 
      actcgcaca aggacgaag acttcgtca cgcaaaacc 
      tcctcccgg tccttgaag gcggccccc aaccctaca 
      aactctgtc tccactacc gcaattgga tcccaggcg 
      aatttatca cgactcaag tcacaaact cggttctcg 
      aatcgcgcc gaacgcttg tggtgcttt cgcccaact 
      tcctggaaa gtgtttggg ggaaaatgg agttccgga 
      ccgctcaca cgcaagcca tgaccgaag gacgggctc 
      gtgttatca ttgtcttgt acggagacg tcgatgtgg 
      atagtttgg acgacgaac tcaaaacgt acttgaaaa 
      caaacactt atgtgaagt ggggcgatc cctacttct 
      ggaacaagt tgaaatatg ctttgccgc acactaaac 
      gaaacgtcg caaatcagc aaaaaatgg caaatgtta 
      tgaactgta tcgacgata agtttaagt tagttgtgc 
      ccgttacga atagtccag gtagtactc cgccagttc 
      tggcacttg atgcaaata tgttgaaac atccactca 
      acttcgtgc ctagtcaag aaatggata ctcctcctg 
      ccgaagctg ggactttgc tggtttcga ctggtctct 
      aagactgtg tttttatta ttctgtaca aatttattg 
      agtaaggtg ccaaaagat tgtatgtaa gtatgtaca 
      aatgtttga attaacgta ctgttaaaa gtgtaaata 
      aaaataaca tgttaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY