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Gene information
General information
TC004437 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4942518..4947377
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004437
    iBeetle screen iB_00696

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 8

    wings orientation irregular - Number of animals showing the phenotype: 8
    wings not closed - Number of animals showing the phenotype: 8
    Adult morphology
    elytra shortened - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection



    elytra shape irregular - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004437 RA
      MATGGNLAL LSVSNKTGL ISLGKNLKD LGFTLVASG 
      GTAKSLREA GLAVKDVSE ISGAPEILG GRVKTLHPA 
      VHGGILARL IPSDQEDLK RQKIDMIRV VVCNLYPFV 
      ETVSNPNVT IADAVENID IGGVTLLRA AAKNHSRVT 
      VVCDPSDYD KIITEMRST PNKDTTAQT RQTLALKAF 
      THTSDYDLA ITDYFRKQY SAGVSQLTL RYGMNPHQK 
      PAQIFTTLS KLPLTVVNG APGFINLCD ALNGWQLVK 
      ELKAGLGLP AATSFKHVS PAGAAVAVP LDSTQAKVC 
      MVDDIFSEL TPISTAYAR ARGADRMSS FGDFVALSD 
      PCDVITARI ISREVSDGI IAPGFTPEA LEILRKKKN 
      GNYCVLQID PNYEPDPIE RKVLFGLTL EQQRNNAVI 
      NKSLFTNIV TKNKSLPDG AVRDLIVAT IALKYTQSN 
      SVCYAKDGQ VIGIGAGQQ SRIHCTRLA GDKADNWWI 
      RQHPKVINM KFRKGVKRA EISNAIDNY VNGTVGKDM 
      DRKVFESMY DEVPTELTQ AEKRAWLDQ LKGVAVGSD 
      AFFPFRDNV DRARLSGVS YIGAPAGST NDAEVIVAC 
      NEHNITLAH TNLRLFHH
    CDS sequence
    • >TC004437 RA
      atggcaaca ggtggtaat ttggctctt ttatcggtt 
      tccaacaaa accggtctc atatccctc gggaaaaac 
      cttaaagat ttgggtttt acccttgtg gcaagtggt 
      ggtactgcg aagagtctc agggaggcg gggctcgct 
      gttaaggat gtctcagag atctctgga gctcctgag 
      atactggga ggaagggtg aagacgctg catccggca 
      gtgcacggg gggatttta gctagactc atcccttca 
      gatcaggag gatttaaaa agacaaaaa atcgatatg 
      atccgagtc gtagtttgc aacttgtat ccattcgtt 
      gaaacagtt tcaaaccca aatgttaca atcgccgat 
      gctgttgaa aacatcgac attggcggc gttactctg 
      ctcagagcc gccgccaaa aaccactca agagtcacc 
      gtagtctgt gacccttca gactacgat aaaatcatc 
      acagaaatg cgtagcacc cccaataaa gacaccaca 
      gctcagact cgtcaaact ctggctctt aaggccttc 
      acacacacc tcagactat gacctagcc attacagac 
      tatttcaga aaacagtat tcggccgga gtttcgcaa 
      cttacgttg cgttatggc atgaatccg caccaaaag 
      ccggcgcaa atttttact actttatcc aaactaccg 
      ttaacagta gtcaacgga gcccccggt tttattaac 
      ttgtgtgat gccctcaat ggatggcag ttggttaag 
      gaacttaag gcgggtttg gggctcccg gcagctacg 
      agttttaaa cacgtatcg ccagcggga gcggctgtg 
      gctgttcct ctagatagt actcaggcg aaagtatgt 
      atggtcgac gatattttc tcggaacta acccccatc 
      tctacggcc tatgcgcga gcccgtgga gcggaccgt 
      atgtcatct ttcggtgac tttgtcgct ctttccgac 
      ccttgtgac gtaatcacg gcccgaatc atctccaga 
      gaggtgtcc gatggaatc atcgctcca ggtttcaca 
      cctgaagct ttggaaatt ttgaggaag aagaagaat 
      ggcaattac tgtgtcctt caaatagac cccaattac 
      gaacccgat ccaattgaa cgcaaagtc ctgttcggc 
      cttactttg gaacagcaa agaaacaac gctgttatt 
      aacaaatcg ctctttacc aacattgtc accaagaat 
      aagtctttg ccggatgga gccgttcgt gacttgatt 
      gtggccacc atagccttg aaatacaca caaagtaac 
      tcggtttgt tacgcgaaa gatgggcaa gttattggg 
      attggagcg ggacaacaa tctaggatt cattgtacg 
      aggcttgca ggggataag gctgataac tggtggatc 
      agacagcac ccgaaagtt atcaacatg aagttcagg 
      aaaggggtg aaaagggct gagatttca aacgcgata 
      gataactac gttaacggg actgttggc aaagatatg 
      gataggaaa gtgttcgaa tcgatgtat gatgaagta 
      ccgactgag ttgactcaa gccgagaaa agggcatgg 
      ttggatcaa ttgaagggt gttgctgtt gggtcggat 
      gcgtttttc cccttccgg gataacgtg gatagggcc 
      cgtctgagt ggggtctcc tacattggg gctcccgcc 
      ggttcgaca aatgacgcc gaagtcatt gttgcgtgc 
      aacgaacac aacattact ttggcccac acaaacttg 
      cggctcttc caccactaa
    mRNA sequence
    • >TC004437 RA
      gttaatttt tgtagatac gtaaaattt ccacaaatt 
      agggcaact ttccaactt cgtttttaa ataaaattc 
      attgttaaa ttatacgga ataacttta tttgctgtt 
      ctctttctc cccgcctta aatcagttg taaacatcc 
      acatttagc gaccaattt aaacaatta agttcaccg 
      gtaccacgt gggtaccac ttattcctt cgtctttct 
      cttcttttg ttcgtcttg ttccctcca gcttatttg 
      cgtgtattt taagacatg gcaacaggt ggtaatttg 
      gctctttta tcggtttcc aacaaaacc ggtctcata 
      tccctcggg aaaaacctt aaagatttg ggttttacc 
      cttgtggca agtggtggt actgcgaag agtctcagg 
      gaggcgggg ctcgctgtt aaggatgtc tcagagatc 
      tctggagct cctgagata ctgggagga agggtgaag 
      acgctgcat ccggcagtg cacgggggg attttagct 
      agactcatc ccttcagat caggaggat ttaaaaaga 
      caaaaaatc gatatgatc cgagtcgta gtttgcaac 
      ttgtatcca ttcgttgaa acagtttca aacccaaat 
      gttacaatc gccgatgct gttgaaaac atcgacatt 
      ggcggcgtt actctgctc agagccgcc gccaaaaac 
      cactcaaga gtcaccgta gtctgtgac ccttcagac 
      tacgataaa atcatcaca gaaatgcgt agcaccccc 
      aataaagac accacagct cagactcgt caaactctg 
      gctcttaag gccttcaca cacacctca gactatgac 
      ctagccatt acagactat ttcagaaaa cagtattcg 
      gccggagtt tcgcaactt acgttgcgt tatggcatg 
      aatccgcac caaaagccg gcgcaaatt tttactact 
      ttatccaaa ctaccgtta acagtagtc aacggagcc 
      cccggtttt attaacttg tgtgatgcc ctcaatgga 
      tggcagttg gttaaggaa cttaaggcg ggtttgggg 
      ctcccggca gctacgagt tttaaacac gtatcgcca 
      gcgggagcg gctgtggct gttcctcta gatagtact 
      caggcgaaa gtatgtatg gtcgacgat attttctcg 
      gaactaacc cccatctct acggcctat gcgcgagcc 
      cgtggagcg gaccgtatg tcatctttc ggtgacttt 
      gtcgctctt tccgaccct tgtgacgta atcacggcc 
      cgaatcatc tccagagag gtgtccgat ggaatcatc 
      gctccaggt ttcacacct gaagctttg gaaattttg 
      aggaagaag aagaatggc aattactgt gtccttcaa 
      atagacccc aattacgaa cccgatcca attgaacgc 
      aaagtcctg ttcggcctt actttggaa cagcaaaga 
      aacaacgct gttattaac aaatcgctc tttaccaac 
      attgtcacc aagaataag tctttgccg gatggagcc 
      gttcgtgac ttgattgtg gccaccata gccttgaaa 
      tacacacaa agtaactcg gtttgttac gcgaaagat 
      gggcaagtt attgggatt ggagcggga caacaatct 
      aggattcat tgtacgagg cttgcaggg gataaggct 
      gataactgg tggatcaga cagcacccg aaagttatc 
      aacatgaag ttcaggaaa ggggtgaaa agggctgag 
      atttcaaac gcgatagat aactacgtt aacgggact 
      gttggcaaa gatatggat aggaaagtg ttcgaatcg 
      atgtatgat gaagtaccg actgagttg actcaagcc 
      gagaaaagg gcatggttg gatcaattg aagggtgtt 
      gctgttggg tcggatgcg tttttcccc ttccgggat 
      aacgtggat agggcccgt ctgagtggg gtctcctac 
      attggggct cccgccggt tcgacaaat gacgccgaa 
      gtcattgtt gcgtgcaac gaacacaac attactttg 
      gcccacaca aacttgcgg ctcttccac cactaattt 
      tacgaacta tatttttgt atacttacc taaagcatg 
      tagatattg tactcgaat aaacgaaat aaaaatatt 
      tttccaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY