Status
Please wait ...
Gene information
General information
TC004429 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid6: 3164156..3165535
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC004429
      iBeetle screen iB_00694
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC004429 RA
        MNSRHKNES LEASLKESL VVNPLGTKV RQATSSQLQ 
        QKPKTAKLV KASQSLHRG RPGQAQFPV KRVMFGVEQ 
        NFAVKTRKK PSTLDFIEK LKKIETKTE KSDFLEQAN 
        DEIDIASKQ LEALRKLLT QKLQTQAEL KAQQEKNEL 
        RNKQKTLMD NCNRQFEKM QKQYLNKTK IRFKSLKDV 
        ENMTLPRPK DAKQDKNVK TLYQGVVAA TSSENFDFS 
        AFDKNQDLD KAGPLLKTQ VVSVASQHE ILAKPAKKA 
        SPKAPQKVR FDDSILNSV HYSPAGSNL QSPERSDGP 
        DVANIKRLL SVPAEDLGV SRFRKKIDE SCLTTENFS 
        TKYKEGRRK LNLLLHGKE KLDNVQFDD AKLEESVLK 
        SAFLSKEFC SGENEASRR NRQLVESVM LKLHENKTG 
        RRQDRKLSA VLKTVLQNS LFSELAK
      CDS sequence
      • >TC004429 RA
        atgaacagc agacacaaa aacgagtcc ttggaagca 
        tccttgaaa gaaagctta gtcgtaaac ccactcggt 
        acaaaagta cgacaagca acaagttcg caactacaa 
        caaaaacca aaaactgca aaattagtc aaagcttcg 
        caaagttta cacagagga agaccaggt caagcccag 
        ttccctgta aaacgagtc atgttcggc gtggagcag 
        aatttcgca gtgaagaca cgtaagaaa ccctcaact 
        ttggatttc atcgaaaaa ttgaaaaaa atcgaaaca 
        aaaactgaa aaatccgac tttttggaa caagcaaat 
        gacgaaatt gacatcgcg tccaaacaa ttagaagca 
        ctgcgtaaa ttactaacg cagaagctc caaactcaa 
        gctgaactt aaagcccaa caagagaaa aatgagctt 
        cgcaacaaa cagaaaacc ttgatggat aactgcaat 
        cggcagttt gaaaaaatg caaaaacag tacttgaac 
        aagacaaaa atacgcttc aagtcgtta aaagacgtt 
        gaaaatatg acactaccg cggccaaaa gacgccaaa 
        caagacaaa aacgttaaa acgctttat caaggtgtg 
        gtggcggct acgagtagc gaaaatttc gattttagt 
        gcttttgat aaaaaccaa gatttggac aaggccggg 
        cctttgctc aagacacag gtggtttct gtggcttcg 
        caacacgag attttggca aaacctgcc aaaaaagcc 
        tcgccaaaa gcgccacag aaagtgaga tttgacgac 
        tcaatcctc aattccgtt cactactct ccagctgga 
        tcaaatttg caaagtcct gaacgaagt gatggtcca 
        gacgttgca aatatcaag cgacttttg tcagtacct 
        gctgaagat ttgggtgtt tcccgtttt cgaaaaaaa 
        atcgacgag tcttgtcta acaactgaa aacttttcg 
        acaaagtac aaagaaggg cgtcgtaaa ctcaatttg 
        ctcctccac ggtaaggaa aaattagac aacgtgcag 
        ttcgacgac gctaaactc gaagagtct gttttaaaa 
        tcggcgttc ctaagcaag gaattttgc agcggggaa 
        aatgaagcg tctcgaagg aatcggcag ttggtggag 
        agtgtgatg ttgaagttg cacgaaaac aagaccggg 
        aggcggcaa gaccggaaa ttgtccgca gtgttgaag 
        accgtcttg caaaatagc ttattcagt gaactggca 
        aagtaa
      mRNA sequence
      • >TC004429 RA
        caagcctgg acccacctt aatttctgc aaaacaaaa 
        ttgtgtcat tttggcaat tcattgaca atcacattt 
        caaaaatca tgaacagca gacacaaaa acgagtcct 
        tggaagcat ccttgaaag aaagcttag tcgtaaacc 
        cactcggta caaaagtac gacaagcaa caagttcgc 
        aactacaac aaaaaccaa aaactgcaa aattagtca 
        aagcttcgc aaagtttac acagaggaa gaccaggtc 
        aagcccagt tccctgtaa aacgagtca tgttcggcg 
        tggagcaga atttcgcag tgaagacac gtaagaaac 
        cctcaactt tggatttca tcgaaaaat tgaaaaaaa 
        tcgaaacaa aaactgaaa aatccgact ttttggaac 
        aagcaaatg acgaaattg acatcgcgt ccaaacaat 
        tagaagcac tgcgtaaat tactaacgc agaagctcc 
        aaactcaag ctgaactta aagcccaac aagagaaaa 
        atgagcttc gcaacaaac agaaaacct tgatggata 
        actgcaatc ggcagtttg aaaaaatgc aaaaacagt 
        acttgaaca agacaaaaa tacgcttca agtcgttaa 
        aagacgttg aaaatatga cactaccgc ggccaaaag 
        acgccaaac aagacaaaa acgttaaaa cgctttatc 
        aaggtgtgg tggcggcta cgagtagcg aaaatttcg 
        attttagtg cttttgata aaaaccaag atttggaca 
        aggccgggc ctttgctca agacacagg tggtttctg 
        tggcttcgc aacacgaga ttttggcaa aacctgcca 
        aaaaagcct cgccaaaag cgccacaga aagtgagat 
        ttgacgact caatcctca attccgttc actactctc 
        cagctggat caaatttgc aaagtcctg aacgaagtg 
        atggtccag acgttgcaa atatcaagc gacttttgt 
        cagtacctg ctgaagatt tgggtgttt cccgttttc 
        gaaaaaaaa tcgacgagt cttgtctaa caactgaaa 
        acttttcga caaagtaca aagaagggc gtcgtaaac 
        tcaatttgc tcctccacg gtaaggaaa aattagaca 
        acgtgcagt tcgacgacg ctaaactcg aagagtctg 
        ttttaaaat cggcgttcc taagcaagg aattttgca 
        gcggggaaa atgaagcgt ctcgaagga atcggcagt 
        tggtggaga gtgtgatgt tgaagttgc acgaaaaca 
        agaccggga ggcggcaag accggaaat tgtccgcag 
        tgttgaaga ccgtcttgc aaaatagct tattcagtg 
        aactggcaa agtaaaatt ttgtgtcac aattaaaaa 
        atatcgacc cga