General information | |||
TC032030
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 5281630..5289888 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC032030 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00693 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC032030 RA
MTEEMYFWS PQDQNYAPN YYETDYNQI PAPGPDFQP FDDFGGQIP DVSKFYDPR NVDFEEEPP LLEELEIYP DRIVQKMLA VLNPFRSHG LTDDADYLN KDPDLAGPI FFYLILAVC LFLSGNKAH FGYIYGISV LSCLMMYSL LSLMTAQSV FNVTTVASV LGYSLIPIV GLSVFGVFF SLKGLLGGV LATLSVMWS SISASRLFV AISGDREQQ PLFAYPCAL VSAVYVLLM TSVRCGIIT VGKLAQAVR STKLQQGVL SAIYTKQPN TPKSLSTCV VLLKSAGSG DPPTQPPSK DVSGNDSGG GGGKNRNTL SCPKCGDPC THVETFVSS TRFVKCEKC HHFFVVLSE VDSKKKEGV DTKAGFFRK PPPPPKKIY DYLNRHVVG QEHAKKVLS VAVYNHYKR IYNNNPNGQ GGGNSRQDV AVMEQGLHQ NVTHRDLLH ITGLGHGNV IGANLSGQE PNRNPTSGS EILDRTTHE LKLEKSNIL LLGPTGSGK TLLAQTIAQ CLDVPFAIC DCTTLTQAG YVGEDIESV IGKLLQDAG FSVDRAQIG IVFLDEVDK IGAVPGIHQ LRDVGGEGV QQGMLKMLE GTVVNVPER NSPRKLRGE TIQVDTTNI LFVASGAYN GLERLIQRR NNENYLGFG APVSESQGR RAVSQQASL YQSSQTAEE ENAEKDAAL KQVQARDLI DFGMIPEFV GRFPVLVPF HSLNKNMLV RILTEPKNA LLPQYQRLL AMDQCELSF TPGALDSIA TLAMERKTG ARGLRAIME SLLLEPMFE VPGSGITTV HVTEEYVRG ESGPVYEKR TDIGTADVN DEDDVNTSI RIKQ
- >TC032030 RA
atgaccgaa gaaatgtat ttttggagt ccacaggac cagaactac gcccccaat tactacgag actgactat aaccaaatt ccggcaccg gggccagac tttcagcct ttcgacgat tttggcggt caaatccct gacgtgtcc aaattctac gatccccga aacgtggat tttgaggag gaaccgccc cttttagaa gaacttgaa atatatccg gaccgtatt gtgcagaaa atgttggcg gttttgaac ccttttcgc agccatggg ttgacggac gatgccgat tatctgaac aaagatcca gacttggcc gggccaatt tttttttac ttgattcta gcggtttgt ttgttcttg tcaggaaat aaggcccat tttgggtat atttacgga atttcagta ttatcgtgc ttgatgatg tatagttta ttgagttta atgacggca cagagtgtc tttaatgta acaacagtg gcaagtgtt ttggggtac agtctgatc cctatcgtt ggtttatca gtttttggg gtgtttttt tccttgaag gggttactt gggggtgtt ttagccact ttgtcggtt atgtggtcc agtatttcg gcttcgaga ctcttcgtt gccatttct ggcgatagg gagcagcag ccccttttt gcgtaccct tgcgcacta gtgtctgcc gtatacgtc cttttgatg acgagtgtt agatgtgga attatcaca gtgggaaaa ctggctcag gctgttcgc tccaccaaa ctacaacaa ggtgtcctg tcagcaatc tacactaaa caacccaac acacccaag tccctttcg acctgcgtt gtcctgctg aaatcggca gggtcgggg gatcccccg actcagccg ccgtcgaaa gacgtgtca gggaatgac agtggcggc ggcggtggt aaaaaccga aacacactc tcatgtccc aaatgtggt gatccttgc acacatgtt gagacgttt gtcagttca acgagattc gtaaaatgc gaaaaatgt catcatttt ttcgtcgtt ttgagcgaa gtggatagt aagaagaag gaaggggtg gacactaag gcagggttt ttccgaaaa ccgccgcca ccgcctaag aaaatctac gattatttg aataggcat gttgtggga caggaacat gcgaagaag gtattatcc gtggctgtg tacaaccat tataagcgt atttataat aataatccg aacggtcaa ggtggaggc aattcgcgc caggatgtg gccgttatg gaacaagga ctccaccaa aatgtcact cacagagat cttcttcat ataacaggc ttggggcat ggtaatgtg atcggtgcg aatctgtct ggtcaagaa ccgaaccga aatcctaca agcggttcg gaaatttta gacagaact acacacgaa ttaaaacta gagaaaagt aatattttg ttacttgga cccactggt tctggtaaa acgcttcta gctcaaact atagcacaa tgtttggat gtacctttt gcaatttgt gattgtact actctaaca caggctgga tacgtcggt gaagatatt gaaagtgtg attgggaaa ttgttacaa gatgcaggg tttagtgta gaccgggct cagatcgga attgttttt cttgatgaa gttgataaa attggagcc gtccctgga attcaccag ttgagggat gttggagga gagggtgta caacaagga atgttgaaa atgttggag ggtacagtc gtaaatgtg cctgaaaga aactcgcct cgaaaatta cgaggagag actatacaa gttgataca acgaatatt ttgttcgtc gctagtggc gcttacaac ggacttgaa cgcttaatt cagagaaga aataacgaa aattactta ggttttggt gcccctgtc tcggaaagc caaggccga agggctgta tcacaacaa gcctccctt tatcaatcc tcgcagaca gccgaggag gaaaatgcg gaaaaagac gcagctttg aaacaagta caagcacgt gatctcatc gattttgga atgataccg gaattcgtg gggaggttc cccgttttg gtccctttc cacagtttg aacaaaaat atgttagtc aggatttta accgaaccg aaaaacgcc ctcttgcct caataccag aggctgcta gcaatggat cagtgcgag ctatctttc acaccgggg gctttagac tcgattgct actttggcc atggaacgg aaaactggg gctcggggg cttagggcc ataatggaa tctttgctc ttggaaccc atgtttgaa gtcccggga tcggggatc acgaccgtt catgtcaca gaggagtac gtgaggggc gagtccggg cctgtgtat gagaagagg acggatatt gggaccgct gatgtgaac gacgaagat gacgttaat acgtcgatt aggataaag caatga
- >TC032030 RA
ttagccaca aaaaattcg gtgtttttt aatttctgg cccaaattg gtggcaaaa tgaccgaag aaatgtatt tttggagtc cacaggacc agaactacg cccccaatt actacgaga ctgactata accaaattc cggcaccgg ggccagact ttcagcctt tcgacgatt ttggcggtc aaatccctg acgtgtcca aattctacg atccccgaa acgtggatt ttgaggagg aaccgcccc ttttagaag aacttgaaa tatatccgg accgtattg tgcagaaaa tgttggcgg ttttgaacc cttttcgca gccatgggt tgacggacg atgccgatt atctgaaca aagatccag acttggccg ggccaattt ttttttact tgattctag cggtttgtt tgttcttgt caggaaata aggcccatt ttgggtata tttacggaa tttcagtat tatcgtgct tgatgatgt atagtttat tgagtttaa tgacggcac agagtgtct ttaatgtaa caacagtgg caagtgttt tggggtaca gtctgatcc ctatcgttg gtttatcag tttttgggg tgttttttt ccttgaagg ggttacttg ggggtgttt tagccactt tgtcggtta tgtggtcca gtatttcgg cttcgagac tcttcgttg ccatttctg gcgataggg agcagcagc ccctttttg cgtaccctt gcgcactag tgtctgccg tatacgtcc ttttgatga cgagtgtta gatgtggaa ttatcacag tgggaaaac tggctcagg ctgttcgct ccaccaaac tacaacaag gtgtcctgt cagcaatct acactaaac aacccaaca cacccaagt ccctttcga cctgcgttg tcctgctga aatcggcag ggtcggggg atcccccga ctcagccgc cgtcgaaag acgtgtcag ggaatgaca gtggcggcg gcggtggta aaaaccgaa acacactct catgtccca aatgtggtg atccttgca cacatgttg agacgtttg tcagttcaa cgagattcg taaaatgcg aaaaatgtc atcattttt tcgtcgttt tgagcgaag tggatagta agaagaagg aaggggtgg acactaagg cagggtttt tccgaaaac cgccgccac cgcctaaga aaatctacg attatttga ataggcatg ttgtgggac aggaacatg cgaagaagg tattatccg tggctgtgt acaaccatt ataagcgta tttataata ataatccga acggtcaag gtggaggca attcgcgcc aggatgtgg ccgttatgg aacaaggac tccaccaaa atgtcactc acagagatc ttcttcata taacaggct tggggcatg gtaatgtga tcggtgcga atctgtctg gtcaagaac cgaaccgaa atcctacaa gcggttcgg aaattttag acagaacta cacacgaat taaaactag agaaaagta atattttgt tacttggac ccactggtt ctggtaaaa cgcttctag ctcaaacta tagcacaat gtttggatg taccttttg caatttgtg attgtacta ctctaacac aggctggat acgtcggtg aagatattg aaagtgtga ttgggaaat tgttacaag atgcagggt ttagtgtag accgggctc agatcggaa ttgtttttc ttgatgaag ttgataaaa ttggagccg tccctggaa ttcaccagt tgagggatg ttggaggag agggtgtac aacaaggaa tgttgaaaa tgttggagg gtacagtcg taaatgtgc ctgaaagaa actcgcctc gaaaattac gaggagaga ctatacaag ttgatacaa cgaatattt tgttcgtcg ctagtggcg cttacaacg gacttgaac gcttaattc agagaagaa ataacgaaa attacttag gttttggtg cccctgtct cggaaagcc aaggccgaa gggctgtat cacaacaag cctcccttt atcaatcct cgcagacag ccgaggagg aaaatgcgg aaaaagacg cagctttga aacaagtac aagcacgtg atctcatcg attttggaa tgataccgg aattcgtgg ggaggttcc ccgttttgg tccctttcc acagtttga acaaaaata tgttagtca ggattttaa ccgaaccga aaaacgccc tcttgcctc aataccaga ggctgctag caatggatc agtgcgagc tatctttca caccggggg ctttagact cgattgcta ctttggcca tggaacgga aaactgggg ctcgggggc ttagggcca taatggaat ctttgctct tggaaccca tgtttgaag tcccgggat cggggatca cgaccgttc atgtcacag aggagtacg tgaggggcg agtccgggc ctgtgtatg agaagagga cggatattg ggaccgctg atgtgaacg acgaagatg acgttaata cgtcgatta ggataaagc aatgagaat cgccaaggg agtagtttt atacatttt atttttatt ttttaggca ataaattgt tttctgacg t
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
