Status
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Gene information
General information
TC032030
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004427,TC004428
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5281630..5289888
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032030
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00693

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032030 RA
      MTEEMYFWS PQDQNYAPN YYETDYNQI PAPGPDFQP 
      FDDFGGQIP DVSKFYDPR NVDFEEEPP LLEELEIYP 
      DRIVQKMLA VLNPFRSHG LTDDADYLN KDPDLAGPI 
      FFYLILAVC LFLSGNKAH FGYIYGISV LSCLMMYSL 
      LSLMTAQSV FNVTTVASV LGYSLIPIV GLSVFGVFF 
      SLKGLLGGV LATLSVMWS SISASRLFV AISGDREQQ 
      PLFAYPCAL VSAVYVLLM TSVRCGIIT VGKLAQAVR 
      STKLQQGVL SAIYTKQPN TPKSLSTCV VLLKSAGSG 
      DPPTQPPSK DVSGNDSGG GGGKNRNTL SCPKCGDPC 
      THVETFVSS TRFVKCEKC HHFFVVLSE VDSKKKEGV 
      DTKAGFFRK PPPPPKKIY DYLNRHVVG QEHAKKVLS 
      VAVYNHYKR IYNNNPNGQ GGGNSRQDV AVMEQGLHQ 
      NVTHRDLLH ITGLGHGNV IGANLSGQE PNRNPTSGS 
      EILDRTTHE LKLEKSNIL LLGPTGSGK TLLAQTIAQ 
      CLDVPFAIC DCTTLTQAG YVGEDIESV IGKLLQDAG 
      FSVDRAQIG IVFLDEVDK IGAVPGIHQ LRDVGGEGV 
      QQGMLKMLE GTVVNVPER NSPRKLRGE TIQVDTTNI 
      LFVASGAYN GLERLIQRR NNENYLGFG APVSESQGR 
      RAVSQQASL YQSSQTAEE ENAEKDAAL KQVQARDLI 
      DFGMIPEFV GRFPVLVPF HSLNKNMLV RILTEPKNA 
      LLPQYQRLL AMDQCELSF TPGALDSIA TLAMERKTG 
      ARGLRAIME SLLLEPMFE VPGSGITTV HVTEEYVRG 
      ESGPVYEKR TDIGTADVN DEDDVNTSI RIKQ
    CDS sequence
    • >TC032030 RA
      atgaccgaa gaaatgtat ttttggagt ccacaggac 
      cagaactac gcccccaat tactacgag actgactat 
      aaccaaatt ccggcaccg gggccagac tttcagcct 
      ttcgacgat tttggcggt caaatccct gacgtgtcc 
      aaattctac gatccccga aacgtggat tttgaggag 
      gaaccgccc cttttagaa gaacttgaa atatatccg 
      gaccgtatt gtgcagaaa atgttggcg gttttgaac 
      ccttttcgc agccatggg ttgacggac gatgccgat 
      tatctgaac aaagatcca gacttggcc gggccaatt 
      tttttttac ttgattcta gcggtttgt ttgttcttg 
      tcaggaaat aaggcccat tttgggtat atttacgga 
      atttcagta ttatcgtgc ttgatgatg tatagttta 
      ttgagttta atgacggca cagagtgtc tttaatgta 
      acaacagtg gcaagtgtt ttggggtac agtctgatc 
      cctatcgtt ggtttatca gtttttggg gtgtttttt 
      tccttgaag gggttactt gggggtgtt ttagccact 
      ttgtcggtt atgtggtcc agtatttcg gcttcgaga 
      ctcttcgtt gccatttct ggcgatagg gagcagcag 
      ccccttttt gcgtaccct tgcgcacta gtgtctgcc 
      gtatacgtc cttttgatg acgagtgtt agatgtgga 
      attatcaca gtgggaaaa ctggctcag gctgttcgc 
      tccaccaaa ctacaacaa ggtgtcctg tcagcaatc 
      tacactaaa caacccaac acacccaag tccctttcg 
      acctgcgtt gtcctgctg aaatcggca gggtcgggg 
      gatcccccg actcagccg ccgtcgaaa gacgtgtca 
      gggaatgac agtggcggc ggcggtggt aaaaaccga 
      aacacactc tcatgtccc aaatgtggt gatccttgc 
      acacatgtt gagacgttt gtcagttca acgagattc 
      gtaaaatgc gaaaaatgt catcatttt ttcgtcgtt 
      ttgagcgaa gtggatagt aagaagaag gaaggggtg 
      gacactaag gcagggttt ttccgaaaa ccgccgcca 
      ccgcctaag aaaatctac gattatttg aataggcat 
      gttgtggga caggaacat gcgaagaag gtattatcc 
      gtggctgtg tacaaccat tataagcgt atttataat 
      aataatccg aacggtcaa ggtggaggc aattcgcgc 
      caggatgtg gccgttatg gaacaagga ctccaccaa 
      aatgtcact cacagagat cttcttcat ataacaggc 
      ttggggcat ggtaatgtg atcggtgcg aatctgtct 
      ggtcaagaa ccgaaccga aatcctaca agcggttcg 
      gaaatttta gacagaact acacacgaa ttaaaacta 
      gagaaaagt aatattttg ttacttgga cccactggt 
      tctggtaaa acgcttcta gctcaaact atagcacaa 
      tgtttggat gtacctttt gcaatttgt gattgtact 
      actctaaca caggctgga tacgtcggt gaagatatt 
      gaaagtgtg attgggaaa ttgttacaa gatgcaggg 
      tttagtgta gaccgggct cagatcgga attgttttt 
      cttgatgaa gttgataaa attggagcc gtccctgga 
      attcaccag ttgagggat gttggagga gagggtgta 
      caacaagga atgttgaaa atgttggag ggtacagtc 
      gtaaatgtg cctgaaaga aactcgcct cgaaaatta 
      cgaggagag actatacaa gttgataca acgaatatt 
      ttgttcgtc gctagtggc gcttacaac ggacttgaa 
      cgcttaatt cagagaaga aataacgaa aattactta 
      ggttttggt gcccctgtc tcggaaagc caaggccga 
      agggctgta tcacaacaa gcctccctt tatcaatcc 
      tcgcagaca gccgaggag gaaaatgcg gaaaaagac 
      gcagctttg aaacaagta caagcacgt gatctcatc 
      gattttgga atgataccg gaattcgtg gggaggttc 
      cccgttttg gtccctttc cacagtttg aacaaaaat 
      atgttagtc aggatttta accgaaccg aaaaacgcc 
      ctcttgcct caataccag aggctgcta gcaatggat 
      cagtgcgag ctatctttc acaccgggg gctttagac 
      tcgattgct actttggcc atggaacgg aaaactggg 
      gctcggggg cttagggcc ataatggaa tctttgctc 
      ttggaaccc atgtttgaa gtcccggga tcggggatc 
      acgaccgtt catgtcaca gaggagtac gtgaggggc 
      gagtccggg cctgtgtat gagaagagg acggatatt 
      gggaccgct gatgtgaac gacgaagat gacgttaat 
      acgtcgatt aggataaag caatga
    mRNA sequence
    • >TC032030 RA
      ttagccaca aaaaattcg gtgtttttt aatttctgg 
      cccaaattg gtggcaaaa tgaccgaag aaatgtatt 
      tttggagtc cacaggacc agaactacg cccccaatt 
      actacgaga ctgactata accaaattc cggcaccgg 
      ggccagact ttcagcctt tcgacgatt ttggcggtc 
      aaatccctg acgtgtcca aattctacg atccccgaa 
      acgtggatt ttgaggagg aaccgcccc ttttagaag 
      aacttgaaa tatatccgg accgtattg tgcagaaaa 
      tgttggcgg ttttgaacc cttttcgca gccatgggt 
      tgacggacg atgccgatt atctgaaca aagatccag 
      acttggccg ggccaattt ttttttact tgattctag 
      cggtttgtt tgttcttgt caggaaata aggcccatt 
      ttgggtata tttacggaa tttcagtat tatcgtgct 
      tgatgatgt atagtttat tgagtttaa tgacggcac 
      agagtgtct ttaatgtaa caacagtgg caagtgttt 
      tggggtaca gtctgatcc ctatcgttg gtttatcag 
      tttttgggg tgttttttt ccttgaagg ggttacttg 
      ggggtgttt tagccactt tgtcggtta tgtggtcca 
      gtatttcgg cttcgagac tcttcgttg ccatttctg 
      gcgataggg agcagcagc ccctttttg cgtaccctt 
      gcgcactag tgtctgccg tatacgtcc ttttgatga 
      cgagtgtta gatgtggaa ttatcacag tgggaaaac 
      tggctcagg ctgttcgct ccaccaaac tacaacaag 
      gtgtcctgt cagcaatct acactaaac aacccaaca 
      cacccaagt ccctttcga cctgcgttg tcctgctga 
      aatcggcag ggtcggggg atcccccga ctcagccgc 
      cgtcgaaag acgtgtcag ggaatgaca gtggcggcg 
      gcggtggta aaaaccgaa acacactct catgtccca 
      aatgtggtg atccttgca cacatgttg agacgtttg 
      tcagttcaa cgagattcg taaaatgcg aaaaatgtc 
      atcattttt tcgtcgttt tgagcgaag tggatagta 
      agaagaagg aaggggtgg acactaagg cagggtttt 
      tccgaaaac cgccgccac cgcctaaga aaatctacg 
      attatttga ataggcatg ttgtgggac aggaacatg 
      cgaagaagg tattatccg tggctgtgt acaaccatt 
      ataagcgta tttataata ataatccga acggtcaag 
      gtggaggca attcgcgcc aggatgtgg ccgttatgg 
      aacaaggac tccaccaaa atgtcactc acagagatc 
      ttcttcata taacaggct tggggcatg gtaatgtga 
      tcggtgcga atctgtctg gtcaagaac cgaaccgaa 
      atcctacaa gcggttcgg aaattttag acagaacta 
      cacacgaat taaaactag agaaaagta atattttgt 
      tacttggac ccactggtt ctggtaaaa cgcttctag 
      ctcaaacta tagcacaat gtttggatg taccttttg 
      caatttgtg attgtacta ctctaacac aggctggat 
      acgtcggtg aagatattg aaagtgtga ttgggaaat 
      tgttacaag atgcagggt ttagtgtag accgggctc 
      agatcggaa ttgtttttc ttgatgaag ttgataaaa 
      ttggagccg tccctggaa ttcaccagt tgagggatg 
      ttggaggag agggtgtac aacaaggaa tgttgaaaa 
      tgttggagg gtacagtcg taaatgtgc ctgaaagaa 
      actcgcctc gaaaattac gaggagaga ctatacaag 
      ttgatacaa cgaatattt tgttcgtcg ctagtggcg 
      cttacaacg gacttgaac gcttaattc agagaagaa 
      ataacgaaa attacttag gttttggtg cccctgtct 
      cggaaagcc aaggccgaa gggctgtat cacaacaag 
      cctcccttt atcaatcct cgcagacag ccgaggagg 
      aaaatgcgg aaaaagacg cagctttga aacaagtac 
      aagcacgtg atctcatcg attttggaa tgataccgg 
      aattcgtgg ggaggttcc ccgttttgg tccctttcc 
      acagtttga acaaaaata tgttagtca ggattttaa 
      ccgaaccga aaaacgccc tcttgcctc aataccaga 
      ggctgctag caatggatc agtgcgagc tatctttca 
      caccggggg ctttagact cgattgcta ctttggcca 
      tggaacgga aaactgggg ctcgggggc ttagggcca 
      taatggaat ctttgctct tggaaccca tgtttgaag 
      tcccgggat cggggatca cgaccgttc atgtcacag 
      aggagtacg tgaggggcg agtccgggc ctgtgtatg 
      agaagagga cggatattg ggaccgctg atgtgaacg 
      acgaagatg acgttaata cgtcgatta ggataaagc 
      aatgagaat cgccaaggg agtagtttt atacatttt 
      atttttatt ttttaggca ataaattgt tttctgacg 
      t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY