Status
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Gene information
General information
TC032030
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004427,TC004428 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5281630..5289888
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032030
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00693

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032030 RA
      MTEEMYFWS PQDQNYAPN YYETDYNQI PAPGPDFQP 
FDDFGGQIP DVSKFYDPR NVDFEEEPP LLEELEIYP 
DRIVQKMLA VLNPFRSHG LTDDADYLN KDPDLAGPI 
FFYLILAVC LFLSGNKAH FGYIYGISV LSCLMMYSL 
LSLMTAQSV FNVTTVASV LGYSLIPIV GLSVFGVFF 
SLKGLLGGV LATLSVMWS SISASRLFV AISGDREQQ 
PLFAYPCAL VSAVYVLLM TSVRCGIIT VGKLAQAVR 
STKLQQGVL SAIYTKQPN TPKSLSTCV VLLKSAGSG 
DPPTQPPSK DVSGNDSGG GGGKNRNTL SCPKCGDPC 
THVETFVSS TRFVKCEKC HHFFVVLSE VDSKKKEGV 
DTKAGFFRK PPPPPKKIY DYLNRHVVG QEHAKKVLS 
VAVYNHYKR IYNNNPNGQ GGGNSRQDV AVMEQGLHQ 
NVTHRDLLH ITGLGHGNV IGANLSGQE PNRNPTSGS 
EILDRTTHE LKLEKSNIL LLGPTGSGK TLLAQTIAQ 
CLDVPFAIC DCTTLTQAG YVGEDIESV IGKLLQDAG 
FSVDRAQIG IVFLDEVDK IGAVPGIHQ LRDVGGEGV 
QQGMLKMLE GTVVNVPER NSPRKLRGE TIQVDTTNI 
LFVASGAYN GLERLIQRR NNENYLGFG APVSESQGR 
RAVSQQASL YQSSQTAEE ENAEKDAAL KQVQARDLI 
DFGMIPEFV GRFPVLVPF HSLNKNMLV RILTEPKNA 
LLPQYQRLL AMDQCELSF TPGALDSIA TLAMERKTG 
ARGLRAIME SLLLEPMFE VPGSGITTV HVTEEYVRG 
ESGPVYEKR TDIGTADVN DEDDVNTSI RIKQ
    CDS sequence
    • >TC032030 RA
      atgaccgaa gaaatgtat ttttggagt ccacaggac 
cagaactac gcccccaat tactacgag actgactat 
aaccaaatt ccggcaccg gggccagac tttcagcct 
ttcgacgat tttggcggt caaatccct gacgtgtcc 
aaattctac gatccccga aacgtggat tttgaggag 
gaaccgccc cttttagaa gaacttgaa atatatccg 
gaccgtatt gtgcagaaa atgttggcg gttttgaac 
ccttttcgc agccatggg ttgacggac gatgccgat 
tatctgaac aaagatcca gacttggcc gggccaatt 
tttttttac ttgattcta gcggtttgt ttgttcttg 
tcaggaaat aaggcccat tttgggtat atttacgga 
atttcagta ttatcgtgc ttgatgatg tatagttta 
ttgagttta atgacggca cagagtgtc tttaatgta 
acaacagtg gcaagtgtt ttggggtac agtctgatc 
cctatcgtt ggtttatca gtttttggg gtgtttttt 
tccttgaag gggttactt gggggtgtt ttagccact 
ttgtcggtt atgtggtcc agtatttcg gcttcgaga 
ctcttcgtt gccatttct ggcgatagg gagcagcag 
ccccttttt gcgtaccct tgcgcacta gtgtctgcc 
gtatacgtc cttttgatg acgagtgtt agatgtgga 
attatcaca gtgggaaaa ctggctcag gctgttcgc 
tccaccaaa ctacaacaa ggtgtcctg tcagcaatc 
tacactaaa caacccaac acacccaag tccctttcg 
acctgcgtt gtcctgctg aaatcggca gggtcgggg 
gatcccccg actcagccg ccgtcgaaa gacgtgtca 
gggaatgac agtggcggc ggcggtggt aaaaaccga 
aacacactc tcatgtccc aaatgtggt gatccttgc 
acacatgtt gagacgttt gtcagttca acgagattc 
gtaaaatgc gaaaaatgt catcatttt ttcgtcgtt 
ttgagcgaa gtggatagt aagaagaag gaaggggtg 
gacactaag gcagggttt ttccgaaaa ccgccgcca 
ccgcctaag aaaatctac gattatttg aataggcat 
gttgtggga caggaacat gcgaagaag gtattatcc 
gtggctgtg tacaaccat tataagcgt atttataat 
aataatccg aacggtcaa ggtggaggc aattcgcgc 
caggatgtg gccgttatg gaacaagga ctccaccaa 
aatgtcact cacagagat cttcttcat ataacaggc 
ttggggcat ggtaatgtg atcggtgcg aatctgtct 
ggtcaagaa ccgaaccga aatcctaca agcggttcg 
gaaatttta gacagaact acacacgaa ttaaaacta 
gagaaaagt aatattttg ttacttgga cccactggt 
tctggtaaa acgcttcta gctcaaact atagcacaa 
tgtttggat gtacctttt gcaatttgt gattgtact 
actctaaca caggctgga tacgtcggt gaagatatt 
gaaagtgtg attgggaaa ttgttacaa gatgcaggg 
tttagtgta gaccgggct cagatcgga attgttttt 
cttgatgaa gttgataaa attggagcc gtccctgga 
attcaccag ttgagggat gttggagga gagggtgta 
caacaagga atgttgaaa atgttggag ggtacagtc 
gtaaatgtg cctgaaaga aactcgcct cgaaaatta 
cgaggagag actatacaa gttgataca acgaatatt 
ttgttcgtc gctagtggc gcttacaac ggacttgaa 
cgcttaatt cagagaaga aataacgaa aattactta 
ggttttggt gcccctgtc tcggaaagc caaggccga 
agggctgta tcacaacaa gcctccctt tatcaatcc 
tcgcagaca gccgaggag gaaaatgcg gaaaaagac 
gcagctttg aaacaagta caagcacgt gatctcatc 
gattttgga atgataccg gaattcgtg gggaggttc 
cccgttttg gtccctttc cacagtttg aacaaaaat 
atgttagtc aggatttta accgaaccg aaaaacgcc 
ctcttgcct caataccag aggctgcta gcaatggat 
cagtgcgag ctatctttc acaccgggg gctttagac 
tcgattgct actttggcc atggaacgg aaaactggg 
gctcggggg cttagggcc ataatggaa tctttgctc 
ttggaaccc atgtttgaa gtcccggga tcggggatc 
acgaccgtt catgtcaca gaggagtac gtgaggggc 
gagtccggg cctgtgtat gagaagagg acggatatt 
gggaccgct gatgtgaac gacgaagat gacgttaat 
acgtcgatt aggataaag caatga
    mRNA sequence
    • >TC032030 RA
      ttagccaca aaaaattcg gtgtttttt aatttctgg 
cccaaattg gtggcaaaa tgaccgaag aaatgtatt 
tttggagtc cacaggacc agaactacg cccccaatt 
actacgaga ctgactata accaaattc cggcaccgg 
ggccagact ttcagcctt tcgacgatt ttggcggtc 
aaatccctg acgtgtcca aattctacg atccccgaa 
acgtggatt ttgaggagg aaccgcccc ttttagaag 
aacttgaaa tatatccgg accgtattg tgcagaaaa 
tgttggcgg ttttgaacc cttttcgca gccatgggt 
tgacggacg atgccgatt atctgaaca aagatccag 
acttggccg ggccaattt ttttttact tgattctag 
cggtttgtt tgttcttgt caggaaata aggcccatt 
ttgggtata tttacggaa tttcagtat tatcgtgct 
tgatgatgt atagtttat tgagtttaa tgacggcac 
agagtgtct ttaatgtaa caacagtgg caagtgttt 
tggggtaca gtctgatcc ctatcgttg gtttatcag 
tttttgggg tgttttttt ccttgaagg ggttacttg 
ggggtgttt tagccactt tgtcggtta tgtggtcca 
gtatttcgg cttcgagac tcttcgttg ccatttctg 
gcgataggg agcagcagc ccctttttg cgtaccctt 
gcgcactag tgtctgccg tatacgtcc ttttgatga 
cgagtgtta gatgtggaa ttatcacag tgggaaaac 
tggctcagg ctgttcgct ccaccaaac tacaacaag 
gtgtcctgt cagcaatct acactaaac aacccaaca 
cacccaagt ccctttcga cctgcgttg tcctgctga 
aatcggcag ggtcggggg atcccccga ctcagccgc 
cgtcgaaag acgtgtcag ggaatgaca gtggcggcg 
gcggtggta aaaaccgaa acacactct catgtccca 
aatgtggtg atccttgca cacatgttg agacgtttg 
tcagttcaa cgagattcg taaaatgcg aaaaatgtc 
atcattttt tcgtcgttt tgagcgaag tggatagta 
agaagaagg aaggggtgg acactaagg cagggtttt 
tccgaaaac cgccgccac cgcctaaga aaatctacg 
attatttga ataggcatg ttgtgggac aggaacatg 
cgaagaagg tattatccg tggctgtgt acaaccatt 
ataagcgta tttataata ataatccga acggtcaag 
gtggaggca attcgcgcc aggatgtgg ccgttatgg 
aacaaggac tccaccaaa atgtcactc acagagatc 
ttcttcata taacaggct tggggcatg gtaatgtga 
tcggtgcga atctgtctg gtcaagaac cgaaccgaa 
atcctacaa gcggttcgg aaattttag acagaacta 
cacacgaat taaaactag agaaaagta atattttgt 
tacttggac ccactggtt ctggtaaaa cgcttctag 
ctcaaacta tagcacaat gtttggatg taccttttg 
caatttgtg attgtacta ctctaacac aggctggat 
acgtcggtg aagatattg aaagtgtga ttgggaaat 
tgttacaag atgcagggt ttagtgtag accgggctc 
agatcggaa ttgtttttc ttgatgaag ttgataaaa 
ttggagccg tccctggaa ttcaccagt tgagggatg 
ttggaggag agggtgtac aacaaggaa tgttgaaaa 
tgttggagg gtacagtcg taaatgtgc ctgaaagaa 
actcgcctc gaaaattac gaggagaga ctatacaag 
ttgatacaa cgaatattt tgttcgtcg ctagtggcg 
cttacaacg gacttgaac gcttaattc agagaagaa 
ataacgaaa attacttag gttttggtg cccctgtct 
cggaaagcc aaggccgaa gggctgtat cacaacaag 
cctcccttt atcaatcct cgcagacag ccgaggagg 
aaaatgcgg aaaaagacg cagctttga aacaagtac 
aagcacgtg atctcatcg attttggaa tgataccgg 
aattcgtgg ggaggttcc ccgttttgg tccctttcc 
acagtttga acaaaaata tgttagtca ggattttaa 
ccgaaccga aaaacgccc tcttgcctc aataccaga 
ggctgctag caatggatc agtgcgagc tatctttca 
caccggggg ctttagact cgattgcta ctttggcca 
tggaacgga aaactgggg ctcgggggc ttagggcca 
taatggaat ctttgctct tggaaccca tgtttgaag 
tcccgggat cggggatca cgaccgttc atgtcacag 
aggagtacg tgaggggcg agtccgggc ctgtgtatg 
agaagagga cggatattg ggaccgctg atgtgaacg 
acgaagatg acgttaata cgtcgatta ggataaagc 
aatgagaat cgccaaggg agtagtttt atacatttt 
atttttatt ttttaggca ataaattgt tttctgacg 
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