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Gene information
General information
TC004426 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5331540..5333461
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004426
    iBeetle screen iB_00692

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004426 RA
      MRSKKLVHE GCTIYKEKP FVYVLSSKL RTEYCDFCL 
KKGQFMKCS GCHYVYYCG KVCQKDGWS VHKSECRGL 
KRVAPRILP DAARFIARL IHILRKGGD LVKSYYLEN 
CFRMYKDLM SHYPNIKGD QQRMEHFTS LCAVLFEFL 
GDDSLPNSA ELMGMYGRM CINSFNIID QELQCIGTG 
MYLGASVID HSCSPNAVA IFDGPILSI RALQTFQYL 
DWSQIKISY IDILNTTKD RQSELEAAY YFLCKCPKC 
LEPEPPEIN AAACPNEKC DNHIDTEII TPGDKCAKC 
DTVVSETFL KRFKEVIEF TDLHLQNMK QLAYFDVCE 
ICLKKQEGV LHKFNIKHV KTLDLAFQS SIDFQKWDF 
ARKFALELV DAFYKYYGH VHPITGLLH LKLGKILLF 
EENDQLALE HLTKAYQIL KIIHGVGSH LFKDELVPL 
LQQARAAIA N
    CDS sequence
    • >TC004426 RA
      atgcgatcg aaaaaactc gtccatgag ggttgtacc 
atctataaa gaaaagccc ttcgtttac gtccttagt 
tcgaaattg cggacggaa tattgcgat ttttgcctc 
aaaaagggg cagtttatg aaatgctca ggttgccac 
tatgtgtat tattgcgga aaagtgtgt cagaaggat 
gggtggagt gtgcataaa tcagaatgt aggggcttg 
aagcgagtg gcccccagg attttaccg gatgctgcg 
aggtttatc gcgagatta atccacatt ttgaggaaa 
gggggcgat ttggtcaag tcgtattat ttggagaat 
tgtttcagg atgtataag gatttaatg tcgcattat 
ccgaatata aaaggggac cagcaacga atggagcat 
ttcacgtca ctgtgtgct gttttattt gaattttta 
ggcgatgat agtcttcca aattcggct gaattgatg 
ggaatgtat ggacgtatg tgtataaac agctttaac 
ataattgat caggagctg caatgtata ggaacaggg 
atgtatcta ggagcttct gtaattgat cattcttgt 
agccctaat gctgtggcg atttttgat ggaccaatt 
ttatccatt cgagcttta caaacgttc caatattta 
gactggtct cagataaaa attagttat atcgacatt 
ttaaacaca acgaaagat cgccaaagt gaattagaa 
gccgcttat tatttcttg tgcaaatgt ccgaaatgt 
ctcgagcct gaaccacct gaaataaac gcagcggcg 
tgtcctaat gaaaaatgt gacaatcac atcgatacc 
gaaattata actcctggc gataaatgt gcgaaatgt 
gacacagtt gtaagcgaa acgttccta aagcgtttt 
aaggaagta atcgagttc accgatttg catttacaa 
aacatgaaa caattagct tattttgac gtttgtgag 
atttgttta aagaagcaa gaaggtgtc ttgcacaaa 
tttaacata aaacacgtc aagactctg gatttggcc 
tttcaatct agtattgac tttcaaaaa tgggatttt 
gccagaaaa ttcgccctt gagctcgtt gacgctttc 
tataaatac tacggacat gtgcatccg attactggg 
ttattgcat ttgaagttg ggcaaaatc ttgctgttt 
gaggaaaat gatcagttg gcgttggaa catctgacc 
aaggcctat cagatattg aagatcatt catggggtt 
ggtagtcat ttgtttaag gatgagttg gtgccattg 
ttgcaacaa gcacgggca gcaatagcg aattaa
    mRNA sequence
    • >TC004426 RA
      cttcgtctc gtagagtcc gaaatgcga tcgaaaaaa 
ctcgtccat gagggttgt accatctat aaagaaaag 
cccttcgtt tacgtcctt agttcgaaa ttgcggacg 
gaatattgc gatttttgc ctcaaaaag gggcagttt 
atgaaatgc tcaggttgc cactatgtg tattattgc 
ggaaaagtg tgtcagaag gatgggtgg agtgtgcat 
aaatcagaa tgtaggggc ttgaagcga gtggccccc 
aggatttta ccggatgct gcgaggttt atcgcgaga 
ttaatccac attttgagg aaagggggc gatttggtc 
aagtcgtat tatttggag aattgtttc aggatgtat 
aaggattta atgtcgcat tatccgaat ataaaaggg 
gaccagcaa cgaatggag catttcacg tcactgtgt 
gctgtttta tttgaattt ttaggcgat gatagtctt 
ccaaattcg gctgaattg atgggaatg tatggacgt 
atgtgtata aacagcttt aacataatt gatcaggag 
ctgcaatgt ataggaaca gggatgtat ctaggagct 
tctgtaatt gatcattct tgtagccct aatgctgtg 
gcgattttt gatggacca attttatcc attcgagct 
ttacaaacg ttccaatat ttagactgg tctcagata 
aaaattagt tatatcgac attttaaac acaacgaaa 
gatcgccaa agtgaatta gaagccgct tattatttc 
ttgtgcaaa tgtccgaaa tgtctcgag cctgaacca 
cctgaaata aacgcagcg gcgtgtcct aatgaaaaa 
tgtgacaat cacatcgat accgaaatt ataactcct 
ggcgataaa tgtgcgaaa tgtgacaca gttgtaagc 
gaaacgttc ctaaagcgt tttaaggaa gtaatcgag 
ttcaccgat ttgcattta caaaacatg aaacaatta 
gcttatttt gacgtttgt gagatttgt ttaaagaag 
caagaaggt gtcttgcac aaatttaac ataaaacac 
gtcaagact ctggatttg gcctttcaa tctagtatt 
gactttcaa aaatgggat tttgccaga aaattcgcc 
cttgagctc gttgacgct ttctataaa tactacgga 
catgtgcat ccgattact gggttattg catttgaag 
ttgggcaaa atcttgctg tttgaggaa aatgatcag 
ttggcgttg gaacatctg accaaggcc tatcagata 
ttgaagatc attcatggg gttggtagt catttgttt 
aaggatgag ttggtgcca ttgttgcaa caagcacgg 
gcagcaata gcgaattaa aaactgatt gaaaaaaac 
tgttttaat aagtcgaaa ataaattta atataaaat 
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