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Gene information
General information
TC004426 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5331540..5333461
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004426
    iBeetle screen iB_00692

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004426 RA
      MRSKKLVHE GCTIYKEKP FVYVLSSKL RTEYCDFCL 
      KKGQFMKCS GCHYVYYCG KVCQKDGWS VHKSECRGL 
      KRVAPRILP DAARFIARL IHILRKGGD LVKSYYLEN 
      CFRMYKDLM SHYPNIKGD QQRMEHFTS LCAVLFEFL 
      GDDSLPNSA ELMGMYGRM CINSFNIID QELQCIGTG 
      MYLGASVID HSCSPNAVA IFDGPILSI RALQTFQYL 
      DWSQIKISY IDILNTTKD RQSELEAAY YFLCKCPKC 
      LEPEPPEIN AAACPNEKC DNHIDTEII TPGDKCAKC 
      DTVVSETFL KRFKEVIEF TDLHLQNMK QLAYFDVCE 
      ICLKKQEGV LHKFNIKHV KTLDLAFQS SIDFQKWDF 
      ARKFALELV DAFYKYYGH VHPITGLLH LKLGKILLF 
      EENDQLALE HLTKAYQIL KIIHGVGSH LFKDELVPL 
      LQQARAAIA N
    CDS sequence
    • >TC004426 RA
      atgcgatcg aaaaaactc gtccatgag ggttgtacc 
      atctataaa gaaaagccc ttcgtttac gtccttagt 
      tcgaaattg cggacggaa tattgcgat ttttgcctc 
      aaaaagggg cagtttatg aaatgctca ggttgccac 
      tatgtgtat tattgcgga aaagtgtgt cagaaggat 
      gggtggagt gtgcataaa tcagaatgt aggggcttg 
      aagcgagtg gcccccagg attttaccg gatgctgcg 
      aggtttatc gcgagatta atccacatt ttgaggaaa 
      gggggcgat ttggtcaag tcgtattat ttggagaat 
      tgtttcagg atgtataag gatttaatg tcgcattat 
      ccgaatata aaaggggac cagcaacga atggagcat 
      ttcacgtca ctgtgtgct gttttattt gaattttta 
      ggcgatgat agtcttcca aattcggct gaattgatg 
      ggaatgtat ggacgtatg tgtataaac agctttaac 
      ataattgat caggagctg caatgtata ggaacaggg 
      atgtatcta ggagcttct gtaattgat cattcttgt 
      agccctaat gctgtggcg atttttgat ggaccaatt 
      ttatccatt cgagcttta caaacgttc caatattta 
      gactggtct cagataaaa attagttat atcgacatt 
      ttaaacaca acgaaagat cgccaaagt gaattagaa 
      gccgcttat tatttcttg tgcaaatgt ccgaaatgt 
      ctcgagcct gaaccacct gaaataaac gcagcggcg 
      tgtcctaat gaaaaatgt gacaatcac atcgatacc 
      gaaattata actcctggc gataaatgt gcgaaatgt 
      gacacagtt gtaagcgaa acgttccta aagcgtttt 
      aaggaagta atcgagttc accgatttg catttacaa 
      aacatgaaa caattagct tattttgac gtttgtgag 
      atttgttta aagaagcaa gaaggtgtc ttgcacaaa 
      tttaacata aaacacgtc aagactctg gatttggcc 
      tttcaatct agtattgac tttcaaaaa tgggatttt 
      gccagaaaa ttcgccctt gagctcgtt gacgctttc 
      tataaatac tacggacat gtgcatccg attactggg 
      ttattgcat ttgaagttg ggcaaaatc ttgctgttt 
      gaggaaaat gatcagttg gcgttggaa catctgacc 
      aaggcctat cagatattg aagatcatt catggggtt 
      ggtagtcat ttgtttaag gatgagttg gtgccattg 
      ttgcaacaa gcacgggca gcaatagcg aattaa
    mRNA sequence
    • >TC004426 RA
      cttcgtctc gtagagtcc gaaatgcga tcgaaaaaa 
      ctcgtccat gagggttgt accatctat aaagaaaag 
      cccttcgtt tacgtcctt agttcgaaa ttgcggacg 
      gaatattgc gatttttgc ctcaaaaag gggcagttt 
      atgaaatgc tcaggttgc cactatgtg tattattgc 
      ggaaaagtg tgtcagaag gatgggtgg agtgtgcat 
      aaatcagaa tgtaggggc ttgaagcga gtggccccc 
      aggatttta ccggatgct gcgaggttt atcgcgaga 
      ttaatccac attttgagg aaagggggc gatttggtc 
      aagtcgtat tatttggag aattgtttc aggatgtat 
      aaggattta atgtcgcat tatccgaat ataaaaggg 
      gaccagcaa cgaatggag catttcacg tcactgtgt 
      gctgtttta tttgaattt ttaggcgat gatagtctt 
      ccaaattcg gctgaattg atgggaatg tatggacgt 
      atgtgtata aacagcttt aacataatt gatcaggag 
      ctgcaatgt ataggaaca gggatgtat ctaggagct 
      tctgtaatt gatcattct tgtagccct aatgctgtg 
      gcgattttt gatggacca attttatcc attcgagct 
      ttacaaacg ttccaatat ttagactgg tctcagata 
      aaaattagt tatatcgac attttaaac acaacgaaa 
      gatcgccaa agtgaatta gaagccgct tattatttc 
      ttgtgcaaa tgtccgaaa tgtctcgag cctgaacca 
      cctgaaata aacgcagcg gcgtgtcct aatgaaaaa 
      tgtgacaat cacatcgat accgaaatt ataactcct 
      ggcgataaa tgtgcgaaa tgtgacaca gttgtaagc 
      gaaacgttc ctaaagcgt tttaaggaa gtaatcgag 
      ttcaccgat ttgcattta caaaacatg aaacaatta 
      gcttatttt gacgtttgt gagatttgt ttaaagaag 
      caagaaggt gtcttgcac aaatttaac ataaaacac 
      gtcaagact ctggatttg gcctttcaa tctagtatt 
      gactttcaa aaatgggat tttgccaga aaattcgcc 
      cttgagctc gttgacgct ttctataaa tactacgga 
      catgtgcat ccgattact gggttattg catttgaag 
      ttgggcaaa atcttgctg tttgaggaa aatgatcag 
      ttggcgttg gaacatctg accaaggcc tatcagata 
      ttgaagatc attcatggg gttggtagt catttgttt 
      aaggatgag ttggtgcca ttgttgcaa caagcacgg 
      gcagcaata gcgaattaa aaactgatt gaaaaaaac 
      tgttttaat aagtcgaaa ataaattta atataaaat 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY