| General information | |||
| TC004422 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 5393040..5397576 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC004422 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00691 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
- >TC004422 RA
MTKKMDEAV RCQIKIDRP IYEHDQLRK DFDYNKSDK KKCLSLDFD TNFKRIVLK TIPAIEWLS HYKWRKNLL ADFISGFTV AIMHIPQGM AYALLGNVP PVVGIYMAF FPVLIYFFL GTSRHNSMG TFAVVCLMT GKAVLEHSD PSYFMKSSI NTTSENPVI ESVHDRYSP MEVATAVTF TVALFQLVM YVLRLGIVS NLLSETLVS GFTTGAAFQ VIASQIKDL LGLKIPKQK GLFVFINTL KCVFDEISE TNTAAVVIS LVTIFILIA NNEVIKPLL AKKSSFPIP IELIAIVLG TLVSRYCSL EEIYSIKVV GEIPSGLPA PNMPPMSLL TSVLLDGFT IAIVSYSIT LSMALIFAQ KLNYEVDAN QELLAQGVG NIFGSFFSC MPFTASLSR STIQQVVGG KTQIASLVS CFLLLIVLL WIGPFFEPL PKSVLASVI VVALKGMVW QIKQLFRFW KMSKMDAIV WLATFLTVV FVSIEIGLL TGVVMSLAT IFVLSLKPY TCLLGSVPG TDLYININR YKGAVEIPG IKIFQYCGG INFATRNIF RSEVLRLVD INPQKELEY RKKLTKYGD EIDVKEPES PNEKIAKLQ RKINRELKC LILDFSSLS HLDPSGASM LQIVTESFQ KIDIPVYIA ACPEPIYEM INKCGLINH KSSIRTFPT VHDAVECAT EIFTLSASP ISTISRI
- >TC004422 RB
MDEAVRCQI KIDRPIYEH DQLRKDFDY NKSDKKKCL SLDFDTNFK RIVLKTIPA IEWLSHYKW RKNLLADFI SGFTVAIMH IPQGMAYAL LGNVPPVVG IYMAFFPVL IYFFLGTSR HNSMGTFAV VCLMTGKAV LEHSDPSYF MKSSINTTS ENPVIESVH DRYSPMEVA TAVTFTVAL FQLVMYVLR LGIVSNLLS ETLVSGFTT GAAFQVIAS QIKDLLGLK IPKQKGLFV FINTLKCVF DEISETNTA AVVISLVTI FILIANNEV IKPLLAKKS SFPIPIELI AIVLGTLVS RYCSLEEIY SIKVVGEIP SGLPAPNMP PMSLLTSVL LDGFTIAIV SYSITLSMA LIFAQKLNY EVDANQELL AQGVGNIFG SFFSCMPFT ASLSRSTIQ QVVGGKTQI ASLVSCFLL LIVLLWIGP FFEPLPKSV LASVIVVAL KGMVWQIKQ LFRFWKMSK MDAIVWLAT FLTVVFVSI EIGLLTGVV MSLATIFVL SLKPYTCLL GSVPGTDLY ININRYKGA VEIPGIKIF QYCGGINFA TRNIFRSEV LRLVDINPQ KELEYRKKL TKYGDEIDV KEPESPNEK IAKLQRKIN RELKCLILD FSSLSHLDP SGASMLQIV TESFQKIDI PVYIAACPE PIYEMINKC GLINHKSSI RTFPTVHDA VECATEIFT LSASPISTI SRI
- >TC004422 RA
atgacaaaa aaaatggac gaggcagtg cggtgccaa atcaaaatc gatagaccc atctacgaa catgaccag ctcaggaaa gactttgat tacaacaaa agcgacaaa aagaaatgt ttgagcctc gattttgac acaaatttc aaacgaatc gtcctcaaa acaatccct gcgatcgaa tggctgagt cattacaaa tggcgaaaa aatttgttg gccgatttt atctcagga ttcactgtt gccataatg cacattccg caaggaatg gcctacgct cttttgggc aacgtcccc ccagttgtg ggcatctac atggccttt ttcccagtc ttgatctac ttttttttg gggacttcc cggcacaat tccatggga accttcgct gttgtttgt ttgatgacc ggaaaagcc gtgctggaa cacagcgat ccctcgtat ttcatgaaa agttcaatt aatacaacg agcgaaaat ccggtcatt gagtcggtc catgaccgc tattctccc atggaagtg gctacagca gtcacattc actgtagcc ttgtttcag cttgtaatg tacgtactg agactagga attgttagt aatttgctt tcggagact ttggttagt ggatttacg actggggcc gcctttcaa gtcattgcc tcacaaatc aaggatttg ttaggattg aaaataccg aaacaaaag ggacttttc gttttcata aatactcta aaatgcgtc tttgacgag atctctgaa acaaacacg gcggctgtt gtcatttct ttagtcact atctttatc ctcattgca aacaacgaa gtgattaag ccacttttg gccaaaaaa tcctcattt ccaattcca atcgagtta atcgcaatc gtattaggg actttagtg tcgcgatat tgcagcctt gaggaaatc tactcgatt aaagtagtc ggagaaatc cccagtggc cttccggcc ccaaatatg cccccaatg tcgctactg acttcagta ctacttgac gggtttacg atagcaata gtttcgtac agtatcacc ctttcaatg gccctaatt ttcgcccaa aaactcaac tacgaagtg gacgccaat caggaactt ttagcgcaa ggggttggc aacattttc ggttcgttt ttctcgtgt atgcccttc acggcgtcg ctttcgcgc tcaacaatt caacaagta gttggggga aaaacacaa atagcgtca ctcgtttcg tgttttctc ctacttata gtcctactc tggattggg ccatttttt gagccgtta ccaaagagt gttttagcc agtgttata gttgtggcc ctcaaagga atggtttgg caaatcaaa cagttgttc agattttgg aaaatgagt aaaatggac gctattgtc tggttggcc acttttctg actgtggtt tttgtcagt attgagatc gggcttttg accggtgtt gtcatgtct ctagctacg atttttgtg cttagtttg aagccctac acttgcttg cttggctca gttccgggc actgatctc tatattaat ataaatcgg tacaaaggg gcggttgaa attccgggg attaaaata tttcagtac tgtgggggg attaatttt gcaactagg aacattttc aggagcgaa gtcttgcgt ctcgttgat ataaacccg caaaaagag ctcgaatat cgcaaaaaa ctgacaaaa tacggagac gaaatcgac gttaaagaa cctgaaagt ccgaatgag aaaatcgca aaactgcaa cgaaaaatc aatcgcgag ttaaagtgt ttgatttta gatttctcg tctttgagt catttggat ccttcgggg gcttcaatg cttcagatt gttactgaa agtttccag aagattgat attcctgtt tacattgca gcctgtcct gaacctatt tacgaaatg attaacaag tgtggtctc atcaatcat aagagttca attcggacg tttcccaca gttcacgat gctgtggag tgtgctact gagatattt actctaagc gcttcccct atttctaca attagccga atataa
- >TC004422 RB
atggacgag gcagtgcgg tgccaaatc aaaatcgat agacccatc tacgaacat gaccagctc aggaaagac tttgattac aacaaaagc gacaaaaag aaatgtttg agcctcgat tttgacaca aatttcaaa cgaatcgtc ctcaaaaca atccctgcg atcgaatgg ctgagtcat tacaaatgg cgaaaaaat ttgttggcc gattttatc tcaggattc actgttgcc ataatgcac attccgcaa ggaatggcc tacgctctt ttgggcaac gtcccccca gttgtgggc atctacatg gcctttttc ccagtcttg atctacttt tttttgggg acttcccgg cacaattcc atgggaacc ttcgctgtt gtttgtttg atgaccgga aaagccgtg ctggaacac agcgatccc tcgtatttc atgaaaagt tcaattaat acaacgagc gaaaatccg gtcattgag tcggtccat gaccgctat tctcccatg gaagtggct acagcagtc acattcact gtagccttg tttcagctt gtaatgtac gtactgaga ctaggaatt gttagtaat ttgctttcg gagactttg gttagtgga tttacgact ggggccgcc tttcaagtc attgcctca caaatcaag gatttgtta ggattgaaa ataccgaaa caaaaggga cttttcgtt ttcataaat actctaaaa tgcgtcttt gacgagatc tctgaaaca aacacggcg gctgttgtc atttcttta gtcactatc tttatcctc attgcaaac aacgaagtg attaagcca cttttggcc aaaaaatcc tcatttcca attccaatc gagttaatc gcaatcgta ttagggact ttagtgtcg cgatattgc agccttgag gaaatctac tcgattaaa gtagtcgga gaaatcccc agtggcctt ccggcccca aatatgccc ccaatgtcg ctactgact tcagtacta cttgacggg tttacgata gcaatagtt tcgtacagt atcaccctt tcaatggcc ctaattttc gcccaaaaa ctcaactac gaagtggac gccaatcag gaactttta gcgcaaggg gttggcaac attttcggt tcgtttttc tcgtgtatg cccttcacg gcgtcgctt tcgcgctca acaattcaa caagtagtt gggggaaaa acacaaata gcgtcactc gtttcgtgt tttctccta cttatagtc ctactctgg attgggcca ttttttgag ccgttacca aagagtgtt ttagccagt gttatagtt gtggccctc aaaggaatg gtttggcaa atcaaacag ttgttcaga ttttggaaa atgagtaaa atggacgct attgtctgg ttggccact tttctgact gtggttttt gtcagtatt gagatcggg cttttgacc ggtgttgtc atgtctcta gctacgatt tttgtgctt agtttgaag ccctacact tgcttgctt ggctcagtt ccgggcact gatctctat attaatata aatcggtac aaaggggcg gttgaaatt ccggggatt aaaatattt cagtactgt ggggggatt aattttgca actaggaac attttcagg agcgaagtc ttgcgtctc gttgatata aacccgcaa aaagagctc gaatatcgc aaaaaactg acaaaatac ggagacgaa atcgacgtt aaagaacct gaaagtccg aatgagaaa atcgcaaaa ctgcaacga aaaatcaat cgcgagtta aagtgtttg attttagat ttctcgtct ttgagtcat ttggatcct tcgggggct tcaatgctt cagattgtt actgaaagt ttccagaag attgatatt cctgtttac attgcagcc tgtcctgaa cctatttac gaaatgatt aacaagtgt ggtctcatc aatcataag agttcaatt cggacgttt cccacagtt cacgatgct gtggagtgt gctactgag atatttact ctaagcgct tcccctatt tctacaatt agccgaata taa
- >TC004422 RA
aatattaat tacttccag ataatgttt gtaactgtt cgttcaaat aaacatgac aaaaaaaat ggacgaggc agtgcggtg ccaaatcaa aatcgatag acccatcta cgaacatga ccagctcag gaaagactt tgattacaa caaaagcga caaaaagaa atgtttgag cctcgattt tgacacaaa tttcaaacg aatcgtcct caaaacaat ccctgcgat cgaatggct gagtcatta caaatggcg aaaaaattt gttggccga ttttatctc aggattcac tgttgccat aatgcacat tccgcaagg aatggccta cgctctttt gggcaacgt ccccccagt tgtgggcat ctacatggc ctttttccc agtcttgat ctacttttt tttggggac ttcccggca caattccat gggaacctt cgctgttgt ttgtttgat gaccggaaa agccgtgct ggaacacag cgatccctc gtatttcat gaaaagttc aattaatac aacgagcga aaatccggt cattgagtc ggtccatga ccgctattc tcccatgga agtggctac agcagtcac attcactgt agccttgtt tcagcttgt aatgtacgt actgagact aggaattgt tagtaattt gctttcgga gactttggt tagtggatt tacgactgg ggccgcctt tcaagtcat tgcctcaca aatcaagga tttgttagg attgaaaat accgaaaca aaagggact tttcgtttt cataaatac tctaaaatg cgtctttga cgagatctc tgaaacaaa cacggcggc tgttgtcat ttctttagt cactatctt tatcctcat tgcaaacaa cgaagtgat taagccact tttggccaa aaaatcctc atttccaat tccaatcga gttaatcgc aatcgtatt agggacttt agtgtcgcg atattgcag ccttgagga aatctactc gattaaagt agtcggaga aatccccag tggccttcc ggccccaaa tatgccccc aatgtcgct actgacttc agtactact tgacgggtt tacgatagc aatagtttc gtacagtat caccctttc aatggccct aattttcgc ccaaaaact caactacga agtggacgc caatcagga acttttagc gcaaggggt tggcaacat tttcggttc gtttttctc gtgtatgcc cttcacggc gtcgctttc gcgctcaac aattcaaca agtagttgg gggaaaaac acaaatagc gtcactcgt ttcgtgttt tctcctact tatagtcct actctggat tgggccatt ttttgagcc gttaccaaa gagtgtttt agccagtgt tatagttgt ggccctcaa aggaatggt ttggcaaat caaacagtt gttcagatt ttggaaaat gagtaaaat ggacgctat tgtctggtt ggccacttt tctgactgt ggtttttgt cagtattga gatcgggct tttgaccgg tgttgtcat gtctctagc tacgatttt tgtgcttag tttgaagcc ctacacttg cttgcttgg ctcagttcc gggcactga tctctatat taatataaa tcggtacaa aggggcggt tgaaattcc ggggattaa aatatttca gtactgtgg ggggattaa ttttgcaac taggaacat tttcaggag cgaagtctt gcgtctcgt tgatataaa cccgcaaaa agagctcga atatcgcaa aaaactgac aaaatacgg agacgaaat cgacgttaa agaacctga aagtccgaa tgagaaaat cgcaaaact gcaacgaaa aatcaatcg cgagttaaa gtgtttgat tttagattt ctcgtcttt gagtcattt ggatccttc gggggcttc aatgcttca gattgttac tgaaagttt ccagaagat tgatattcc tgtttacat tgcagcctg tcctgaacc tatttacga aatgattaa caagtgtgg tctcatcaa tcataagag ttcaattcg gacgtttcc cacagttca cgatgctgt ggagtgtgc tactgagat atttactct aagcgcttc ccctatttc tacaattag ccgaatata aatcaaact aaaaataaa taaataatg aaaaagcca
- >TC004422 RB
aatattaat tacttccag ataatgttt gtaactgtt cgttcaaat aaacatgac aaaaaaaat ggacgaggc agtgcggtg ccaaatcaa aatcgatag acccatcta cgaacatga ccagctcag gaaagactt tgattacaa caaaagcga caaaaagaa atgtttgag cctcgattt tgacacaaa tttcaaacg aatcgtcct caaaacaat ccctgcgat cgaatggct gagtcatta caaatggcg aaaaaattt gttggccga ttttatctc aggattcac tgttgccat aatgcacat tccgcaagg aatggccta cgctctttt gggcaacgt ccccccagt tgtgggcat ctacatggc ctttttccc agtcttgat ctacttttt tttggggac ttcccggca caattccat gggaacctt cgctgttgt ttgtttgat gaccggaaa agccgtgct ggaacacag cgatccctc gtatttcat gaaaagttc aattaatac aacgagcga aaatccggt cattgagtc ggtccatga ccgctattc tcccatgga agtggctac agcagtcac attcactgt agccttgtt tcagcttgt aatgtacgt actgagact aggaattgt tagtaattt gctttcgga gactttggt tagtggatt tacgactgg ggccgcctt tcaagtcat tgcctcaca aatcaagga tttgttagg attgaaaat accgaaaca aaagggact tttcgtttt cataaatac tctaaaatg cgtctttga cgagatctc tgaaacaaa cacggcggc tgttgtcat ttctttagt cactatctt tatcctcat tgcaaacaa cgaagtgat taagccact tttggccaa aaaatcctc atttccaat tccaatcga gttaatcgc aatcgtatt agggacttt agtgtcgcg atattgcag ccttgagga aatctactc gattaaagt agtcggaga aatccccag tggccttcc ggccccaaa tatgccccc aatgtcgct actgacttc agtactact tgacgggtt tacgatagc aatagtttc gtacagtat caccctttc aatggccct aattttcgc ccaaaaact caactacga agtggacgc caatcagga acttttagc gcaaggggt tggcaacat tttcggttc gtttttctc gtgtatgcc cttcacggc gtcgctttc gcgctcaac aattcaaca agtagttgg gggaaaaac acaaatagc gtcactcgt ttcgtgttt tctcctact tatagtcct actctggat tgggccatt ttttgagcc gttaccaaa gagtgtttt agccagtgt tatagttgt ggccctcaa aggaatggt ttggcaaat caaacagtt gttcagatt ttggaaaat gagtaaaat ggacgctat tgtctggtt ggccacttt tctgactgt ggtttttgt cagtattga gatcgggct tttgaccgg tgttgtcat gtctctagc tacgatttt tgtgcttag tttgaagcc ctacacttg cttgcttgg ctcagttcc gggcactga tctctatat taatataaa tcggtacaa aggggcggt tgaaattcc ggggattaa aatatttca gtactgtgg ggggattaa ttttgcaac taggaacat tttcaggag cgaagtctt gcgtctcgt tgatataaa cccgcaaaa agagctcga atatcgcaa aaaactgac aaaatacgg agacgaaat cgacgttaa agaacctga aagtccgaa tgagaaaat cgcaaaact gcaacgaaa aatcaatcg cgagttaaa gtgtttgat tttagattt ctcgtcttt gagtcattt ggatccttc gggggcttc aatgcttca gattgttac tgaaagttt ccagaagat tgatattcc tgtttacat tgcagcctg tcctgaacc tatttacga aatgattaa caagtgtgg tctcatcaa tcataagag ttcaattcg gacgtttcc cacagttca cgatgctgt ggagtgtgc tactgagat atttactct aagcgcttc ccctatttc tacaattag ccgaatata aatcaaact aaaaataaa taaataatg aaaaagcca
