Status
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Gene information
General information
TC004422 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5393040..5397576
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004422
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00691

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: bad quality of preparation,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004422 RA
      MTKKMDEAV RCQIKIDRP IYEHDQLRK DFDYNKSDK 
      KKCLSLDFD TNFKRIVLK TIPAIEWLS HYKWRKNLL 
      ADFISGFTV AIMHIPQGM AYALLGNVP PVVGIYMAF 
      FPVLIYFFL GTSRHNSMG TFAVVCLMT GKAVLEHSD 
      PSYFMKSSI NTTSENPVI ESVHDRYSP MEVATAVTF 
      TVALFQLVM YVLRLGIVS NLLSETLVS GFTTGAAFQ 
      VIASQIKDL LGLKIPKQK GLFVFINTL KCVFDEISE 
      TNTAAVVIS LVTIFILIA NNEVIKPLL AKKSSFPIP 
      IELIAIVLG TLVSRYCSL EEIYSIKVV GEIPSGLPA 
      PNMPPMSLL TSVLLDGFT IAIVSYSIT LSMALIFAQ 
      KLNYEVDAN QELLAQGVG NIFGSFFSC MPFTASLSR 
      STIQQVVGG KTQIASLVS CFLLLIVLL WIGPFFEPL 
      PKSVLASVI VVALKGMVW QIKQLFRFW KMSKMDAIV 
      WLATFLTVV FVSIEIGLL TGVVMSLAT IFVLSLKPY 
      TCLLGSVPG TDLYININR YKGAVEIPG IKIFQYCGG 
      INFATRNIF RSEVLRLVD INPQKELEY RKKLTKYGD 
      EIDVKEPES PNEKIAKLQ RKINRELKC LILDFSSLS 
      HLDPSGASM LQIVTESFQ KIDIPVYIA ACPEPIYEM 
      INKCGLINH KSSIRTFPT VHDAVECAT EIFTLSASP 
      ISTISRI
    • >TC004422 RB
      MDEAVRCQI KIDRPIYEH DQLRKDFDY NKSDKKKCL 
      SLDFDTNFK RIVLKTIPA IEWLSHYKW RKNLLADFI 
      SGFTVAIMH IPQGMAYAL LGNVPPVVG IYMAFFPVL 
      IYFFLGTSR HNSMGTFAV VCLMTGKAV LEHSDPSYF 
      MKSSINTTS ENPVIESVH DRYSPMEVA TAVTFTVAL 
      FQLVMYVLR LGIVSNLLS ETLVSGFTT GAAFQVIAS 
      QIKDLLGLK IPKQKGLFV FINTLKCVF DEISETNTA 
      AVVISLVTI FILIANNEV IKPLLAKKS SFPIPIELI 
      AIVLGTLVS RYCSLEEIY SIKVVGEIP SGLPAPNMP 
      PMSLLTSVL LDGFTIAIV SYSITLSMA LIFAQKLNY 
      EVDANQELL AQGVGNIFG SFFSCMPFT ASLSRSTIQ 
      QVVGGKTQI ASLVSCFLL LIVLLWIGP FFEPLPKSV 
      LASVIVVAL KGMVWQIKQ LFRFWKMSK MDAIVWLAT 
      FLTVVFVSI EIGLLTGVV MSLATIFVL SLKPYTCLL 
      GSVPGTDLY ININRYKGA VEIPGIKIF QYCGGINFA 
      TRNIFRSEV LRLVDINPQ KELEYRKKL TKYGDEIDV 
      KEPESPNEK IAKLQRKIN RELKCLILD FSSLSHLDP 
      SGASMLQIV TESFQKIDI PVYIAACPE PIYEMINKC 
      GLINHKSSI RTFPTVHDA VECATEIFT LSASPISTI 
      SRI
    CDS sequence
    • >TC004422 RA
      atgacaaaa aaaatggac gaggcagtg cggtgccaa 
      atcaaaatc gatagaccc atctacgaa catgaccag 
      ctcaggaaa gactttgat tacaacaaa agcgacaaa 
      aagaaatgt ttgagcctc gattttgac acaaatttc 
      aaacgaatc gtcctcaaa acaatccct gcgatcgaa 
      tggctgagt cattacaaa tggcgaaaa aatttgttg 
      gccgatttt atctcagga ttcactgtt gccataatg 
      cacattccg caaggaatg gcctacgct cttttgggc 
      aacgtcccc ccagttgtg ggcatctac atggccttt 
      ttcccagtc ttgatctac ttttttttg gggacttcc 
      cggcacaat tccatggga accttcgct gttgtttgt 
      ttgatgacc ggaaaagcc gtgctggaa cacagcgat 
      ccctcgtat ttcatgaaa agttcaatt aatacaacg 
      agcgaaaat ccggtcatt gagtcggtc catgaccgc 
      tattctccc atggaagtg gctacagca gtcacattc 
      actgtagcc ttgtttcag cttgtaatg tacgtactg 
      agactagga attgttagt aatttgctt tcggagact 
      ttggttagt ggatttacg actggggcc gcctttcaa 
      gtcattgcc tcacaaatc aaggatttg ttaggattg 
      aaaataccg aaacaaaag ggacttttc gttttcata 
      aatactcta aaatgcgtc tttgacgag atctctgaa 
      acaaacacg gcggctgtt gtcatttct ttagtcact 
      atctttatc ctcattgca aacaacgaa gtgattaag 
      ccacttttg gccaaaaaa tcctcattt ccaattcca 
      atcgagtta atcgcaatc gtattaggg actttagtg 
      tcgcgatat tgcagcctt gaggaaatc tactcgatt 
      aaagtagtc ggagaaatc cccagtggc cttccggcc 
      ccaaatatg cccccaatg tcgctactg acttcagta 
      ctacttgac gggtttacg atagcaata gtttcgtac 
      agtatcacc ctttcaatg gccctaatt ttcgcccaa 
      aaactcaac tacgaagtg gacgccaat caggaactt 
      ttagcgcaa ggggttggc aacattttc ggttcgttt 
      ttctcgtgt atgcccttc acggcgtcg ctttcgcgc 
      tcaacaatt caacaagta gttggggga aaaacacaa 
      atagcgtca ctcgtttcg tgttttctc ctacttata 
      gtcctactc tggattggg ccatttttt gagccgtta 
      ccaaagagt gttttagcc agtgttata gttgtggcc 
      ctcaaagga atggtttgg caaatcaaa cagttgttc 
      agattttgg aaaatgagt aaaatggac gctattgtc 
      tggttggcc acttttctg actgtggtt tttgtcagt 
      attgagatc gggcttttg accggtgtt gtcatgtct 
      ctagctacg atttttgtg cttagtttg aagccctac 
      acttgcttg cttggctca gttccgggc actgatctc 
      tatattaat ataaatcgg tacaaaggg gcggttgaa 
      attccgggg attaaaata tttcagtac tgtgggggg 
      attaatttt gcaactagg aacattttc aggagcgaa 
      gtcttgcgt ctcgttgat ataaacccg caaaaagag 
      ctcgaatat cgcaaaaaa ctgacaaaa tacggagac 
      gaaatcgac gttaaagaa cctgaaagt ccgaatgag 
      aaaatcgca aaactgcaa cgaaaaatc aatcgcgag 
      ttaaagtgt ttgatttta gatttctcg tctttgagt 
      catttggat ccttcgggg gcttcaatg cttcagatt 
      gttactgaa agtttccag aagattgat attcctgtt 
      tacattgca gcctgtcct gaacctatt tacgaaatg 
      attaacaag tgtggtctc atcaatcat aagagttca 
      attcggacg tttcccaca gttcacgat gctgtggag 
      tgtgctact gagatattt actctaagc gcttcccct 
      atttctaca attagccga atataa
    • >TC004422 RB
      atggacgag gcagtgcgg tgccaaatc aaaatcgat 
      agacccatc tacgaacat gaccagctc aggaaagac 
      tttgattac aacaaaagc gacaaaaag aaatgtttg 
      agcctcgat tttgacaca aatttcaaa cgaatcgtc 
      ctcaaaaca atccctgcg atcgaatgg ctgagtcat 
      tacaaatgg cgaaaaaat ttgttggcc gattttatc 
      tcaggattc actgttgcc ataatgcac attccgcaa 
      ggaatggcc tacgctctt ttgggcaac gtcccccca 
      gttgtgggc atctacatg gcctttttc ccagtcttg 
      atctacttt tttttgggg acttcccgg cacaattcc 
      atgggaacc ttcgctgtt gtttgtttg atgaccgga 
      aaagccgtg ctggaacac agcgatccc tcgtatttc 
      atgaaaagt tcaattaat acaacgagc gaaaatccg 
      gtcattgag tcggtccat gaccgctat tctcccatg 
      gaagtggct acagcagtc acattcact gtagccttg 
      tttcagctt gtaatgtac gtactgaga ctaggaatt 
      gttagtaat ttgctttcg gagactttg gttagtgga 
      tttacgact ggggccgcc tttcaagtc attgcctca 
      caaatcaag gatttgtta ggattgaaa ataccgaaa 
      caaaaggga cttttcgtt ttcataaat actctaaaa 
      tgcgtcttt gacgagatc tctgaaaca aacacggcg 
      gctgttgtc atttcttta gtcactatc tttatcctc 
      attgcaaac aacgaagtg attaagcca cttttggcc 
      aaaaaatcc tcatttcca attccaatc gagttaatc 
      gcaatcgta ttagggact ttagtgtcg cgatattgc 
      agccttgag gaaatctac tcgattaaa gtagtcgga 
      gaaatcccc agtggcctt ccggcccca aatatgccc 
      ccaatgtcg ctactgact tcagtacta cttgacggg 
      tttacgata gcaatagtt tcgtacagt atcaccctt 
      tcaatggcc ctaattttc gcccaaaaa ctcaactac 
      gaagtggac gccaatcag gaactttta gcgcaaggg 
      gttggcaac attttcggt tcgtttttc tcgtgtatg 
      cccttcacg gcgtcgctt tcgcgctca acaattcaa 
      caagtagtt gggggaaaa acacaaata gcgtcactc 
      gtttcgtgt tttctccta cttatagtc ctactctgg 
      attgggcca ttttttgag ccgttacca aagagtgtt 
      ttagccagt gttatagtt gtggccctc aaaggaatg 
      gtttggcaa atcaaacag ttgttcaga ttttggaaa 
      atgagtaaa atggacgct attgtctgg ttggccact 
      tttctgact gtggttttt gtcagtatt gagatcggg 
      cttttgacc ggtgttgtc atgtctcta gctacgatt 
      tttgtgctt agtttgaag ccctacact tgcttgctt 
      ggctcagtt ccgggcact gatctctat attaatata 
      aatcggtac aaaggggcg gttgaaatt ccggggatt 
      aaaatattt cagtactgt ggggggatt aattttgca 
      actaggaac attttcagg agcgaagtc ttgcgtctc 
      gttgatata aacccgcaa aaagagctc gaatatcgc 
      aaaaaactg acaaaatac ggagacgaa atcgacgtt 
      aaagaacct gaaagtccg aatgagaaa atcgcaaaa 
      ctgcaacga aaaatcaat cgcgagtta aagtgtttg 
      attttagat ttctcgtct ttgagtcat ttggatcct 
      tcgggggct tcaatgctt cagattgtt actgaaagt 
      ttccagaag attgatatt cctgtttac attgcagcc 
      tgtcctgaa cctatttac gaaatgatt aacaagtgt 
      ggtctcatc aatcataag agttcaatt cggacgttt 
      cccacagtt cacgatgct gtggagtgt gctactgag 
      atatttact ctaagcgct tcccctatt tctacaatt 
      agccgaata taa
    mRNA sequence
    • >TC004422 RA
      aatattaat tacttccag ataatgttt gtaactgtt 
      cgttcaaat aaacatgac aaaaaaaat ggacgaggc 
      agtgcggtg ccaaatcaa aatcgatag acccatcta 
      cgaacatga ccagctcag gaaagactt tgattacaa 
      caaaagcga caaaaagaa atgtttgag cctcgattt 
      tgacacaaa tttcaaacg aatcgtcct caaaacaat 
      ccctgcgat cgaatggct gagtcatta caaatggcg 
      aaaaaattt gttggccga ttttatctc aggattcac 
      tgttgccat aatgcacat tccgcaagg aatggccta 
      cgctctttt gggcaacgt ccccccagt tgtgggcat 
      ctacatggc ctttttccc agtcttgat ctacttttt 
      tttggggac ttcccggca caattccat gggaacctt 
      cgctgttgt ttgtttgat gaccggaaa agccgtgct 
      ggaacacag cgatccctc gtatttcat gaaaagttc 
      aattaatac aacgagcga aaatccggt cattgagtc 
      ggtccatga ccgctattc tcccatgga agtggctac 
      agcagtcac attcactgt agccttgtt tcagcttgt 
      aatgtacgt actgagact aggaattgt tagtaattt 
      gctttcgga gactttggt tagtggatt tacgactgg 
      ggccgcctt tcaagtcat tgcctcaca aatcaagga 
      tttgttagg attgaaaat accgaaaca aaagggact 
      tttcgtttt cataaatac tctaaaatg cgtctttga 
      cgagatctc tgaaacaaa cacggcggc tgttgtcat 
      ttctttagt cactatctt tatcctcat tgcaaacaa 
      cgaagtgat taagccact tttggccaa aaaatcctc 
      atttccaat tccaatcga gttaatcgc aatcgtatt 
      agggacttt agtgtcgcg atattgcag ccttgagga 
      aatctactc gattaaagt agtcggaga aatccccag 
      tggccttcc ggccccaaa tatgccccc aatgtcgct 
      actgacttc agtactact tgacgggtt tacgatagc 
      aatagtttc gtacagtat caccctttc aatggccct 
      aattttcgc ccaaaaact caactacga agtggacgc 
      caatcagga acttttagc gcaaggggt tggcaacat 
      tttcggttc gtttttctc gtgtatgcc cttcacggc 
      gtcgctttc gcgctcaac aattcaaca agtagttgg 
      gggaaaaac acaaatagc gtcactcgt ttcgtgttt 
      tctcctact tatagtcct actctggat tgggccatt 
      ttttgagcc gttaccaaa gagtgtttt agccagtgt 
      tatagttgt ggccctcaa aggaatggt ttggcaaat 
      caaacagtt gttcagatt ttggaaaat gagtaaaat 
      ggacgctat tgtctggtt ggccacttt tctgactgt 
      ggtttttgt cagtattga gatcgggct tttgaccgg 
      tgttgtcat gtctctagc tacgatttt tgtgcttag 
      tttgaagcc ctacacttg cttgcttgg ctcagttcc 
      gggcactga tctctatat taatataaa tcggtacaa 
      aggggcggt tgaaattcc ggggattaa aatatttca 
      gtactgtgg ggggattaa ttttgcaac taggaacat 
      tttcaggag cgaagtctt gcgtctcgt tgatataaa 
      cccgcaaaa agagctcga atatcgcaa aaaactgac 
      aaaatacgg agacgaaat cgacgttaa agaacctga 
      aagtccgaa tgagaaaat cgcaaaact gcaacgaaa 
      aatcaatcg cgagttaaa gtgtttgat tttagattt 
      ctcgtcttt gagtcattt ggatccttc gggggcttc 
      aatgcttca gattgttac tgaaagttt ccagaagat 
      tgatattcc tgtttacat tgcagcctg tcctgaacc 
      tatttacga aatgattaa caagtgtgg tctcatcaa 
      tcataagag ttcaattcg gacgtttcc cacagttca 
      cgatgctgt ggagtgtgc tactgagat atttactct 
      aagcgcttc ccctatttc tacaattag ccgaatata 
      aatcaaact aaaaataaa taaataatg aaaaagcca
    • >TC004422 RB
      aatattaat tacttccag ataatgttt gtaactgtt 
      cgttcaaat aaacatgac aaaaaaaat ggacgaggc 
      agtgcggtg ccaaatcaa aatcgatag acccatcta 
      cgaacatga ccagctcag gaaagactt tgattacaa 
      caaaagcga caaaaagaa atgtttgag cctcgattt 
      tgacacaaa tttcaaacg aatcgtcct caaaacaat 
      ccctgcgat cgaatggct gagtcatta caaatggcg 
      aaaaaattt gttggccga ttttatctc aggattcac 
      tgttgccat aatgcacat tccgcaagg aatggccta 
      cgctctttt gggcaacgt ccccccagt tgtgggcat 
      ctacatggc ctttttccc agtcttgat ctacttttt 
      tttggggac ttcccggca caattccat gggaacctt 
      cgctgttgt ttgtttgat gaccggaaa agccgtgct 
      ggaacacag cgatccctc gtatttcat gaaaagttc 
      aattaatac aacgagcga aaatccggt cattgagtc 
      ggtccatga ccgctattc tcccatgga agtggctac 
      agcagtcac attcactgt agccttgtt tcagcttgt 
      aatgtacgt actgagact aggaattgt tagtaattt 
      gctttcgga gactttggt tagtggatt tacgactgg 
      ggccgcctt tcaagtcat tgcctcaca aatcaagga 
      tttgttagg attgaaaat accgaaaca aaagggact 
      tttcgtttt cataaatac tctaaaatg cgtctttga 
      cgagatctc tgaaacaaa cacggcggc tgttgtcat 
      ttctttagt cactatctt tatcctcat tgcaaacaa 
      cgaagtgat taagccact tttggccaa aaaatcctc 
      atttccaat tccaatcga gttaatcgc aatcgtatt 
      agggacttt agtgtcgcg atattgcag ccttgagga 
      aatctactc gattaaagt agtcggaga aatccccag 
      tggccttcc ggccccaaa tatgccccc aatgtcgct 
      actgacttc agtactact tgacgggtt tacgatagc 
      aatagtttc gtacagtat caccctttc aatggccct 
      aattttcgc ccaaaaact caactacga agtggacgc 
      caatcagga acttttagc gcaaggggt tggcaacat 
      tttcggttc gtttttctc gtgtatgcc cttcacggc 
      gtcgctttc gcgctcaac aattcaaca agtagttgg 
      gggaaaaac acaaatagc gtcactcgt ttcgtgttt 
      tctcctact tatagtcct actctggat tgggccatt 
      ttttgagcc gttaccaaa gagtgtttt agccagtgt 
      tatagttgt ggccctcaa aggaatggt ttggcaaat 
      caaacagtt gttcagatt ttggaaaat gagtaaaat 
      ggacgctat tgtctggtt ggccacttt tctgactgt 
      ggtttttgt cagtattga gatcgggct tttgaccgg 
      tgttgtcat gtctctagc tacgatttt tgtgcttag 
      tttgaagcc ctacacttg cttgcttgg ctcagttcc 
      gggcactga tctctatat taatataaa tcggtacaa 
      aggggcggt tgaaattcc ggggattaa aatatttca 
      gtactgtgg ggggattaa ttttgcaac taggaacat 
      tttcaggag cgaagtctt gcgtctcgt tgatataaa 
      cccgcaaaa agagctcga atatcgcaa aaaactgac 
      aaaatacgg agacgaaat cgacgttaa agaacctga 
      aagtccgaa tgagaaaat cgcaaaact gcaacgaaa 
      aatcaatcg cgagttaaa gtgtttgat tttagattt 
      ctcgtcttt gagtcattt ggatccttc gggggcttc 
      aatgcttca gattgttac tgaaagttt ccagaagat 
      tgatattcc tgtttacat tgcagcctg tcctgaacc 
      tatttacga aatgattaa caagtgtgg tctcatcaa 
      tcataagag ttcaattcg gacgtttcc cacagttca 
      cgatgctgt ggagtgtgc tactgagat atttactct 
      aagcgcttc ccctatttc tacaattag ccgaatata 
      aatcaaact aaaaataaa taaataatg aaaaagcca
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