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Gene information
General information
TC031063
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004406
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19715846..19736213
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031063
    iBeetle screen iB_00690

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: bad quality of preparation,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head with protrusion - Penetrance (percentage of animals/eggs on slide): less than 30%

    procephalic head irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031063 RA
      METPSNASA PSNNVKQPW YGSNPGYQS DTKDKSNEN 
      VFMTFQSKD PIISIPLQE KHPKDKVPG SGGGHGHGI 
      SVEHPTSYG ETLMHLFKG NVGSGIFAM GDAIRNAGI 
      IVGPGIVLL LGVICVHCQ HLLLSAALK MKSMKEVSV 
      PPDFAETVE LCFATGPPA IKKISKIMK IVVNTFLCI 
      TQLGFCCVY FVFISENVK KVLDYYGYE LDVHFHMAI 
      ILLPILCTS LVRNLKYLA PFSTVANIF MLMGLIITV 
      YYTTQDLPS FSERNYYAE PSQLPLFFG TAVFAFEGI 
      GLVLPLQNE MRKPSDFKK PFGVLNVGM CVVTVLYIL 
      IGTLSYLKY GEDIEGSVT LNLPKGDIL AQSVKIIIS 
      LGILLTYAL QFYIAVEIM FPTLERMLG PFKYPVFAE 
      LSFRSVLVL ITFILAEAI PFLNHFISL VGAVSSATL 
      ALIFPPILD LVTSYSFGD LKCTTVVKN VIILIVGVV 
      GCITGTYES INSIVDAFN NSSKQEH
    CDS sequence
    • >TC031063 RA
      atggaaacg ccaagcaat gcaagtgcc ccttcaaac 
      aacgtcaag cagccatgg tacggctca aaccctggc 
      taccaatcg gacacaaaa gataaaagc aatgaaaat 
      gtatttatg acttttcag tctaaagac ccaatcatt 
      tccatcccc ttgcaagaa aagcacccc aaagacaaa 
      gtcccggga agcgggggt ggtcatgga catggcatt 
      tccgtagaa catcctacg tcatatgga gagaccttg 
      atgcacctt ttcaaaggg aatgttgga tcggggatt 
      tttgccatg ggagatgcc atccggaac gctggaatt 
      atcgttgga cccggaatt gtcctgctg ttaggggtc 
      atttgtgtt cattgccaa cacttattg ttatcagcg 
      gccctaaaa atgaaatcg atgaaagaa gtctccgtc 
      cctccggac tttgccgaa acagtggag ctctgcttc 
      gccaccggc cctccagcc ataaaaaaa atttctaaa 
      attatgaaa attgttgta aatactttt ttgtgtata 
      acccagttg gggttttgc tgtgtctac tttgttttt 
      atttcggaa aacgtcaag aaggttttg gattattat 
      ggctatgag ctggacgtc cattttcac atggcaata 
      atccttctg ccgatcctt tgtacgtct ctagttcgt 
      aatttaaaa tatctcgca ccgttttcc acggttgcg 
      aatattttc atgttgatg ggccttatc atcacagtt 
      tattacacg acacaagac cttccgtcc ttttcggaa 
      cgaaattac tacgcggaa ccgtcacaa ctgccgctt 
      ttctttgga acagcagtg tttgccttc gaaggaatc 
      ggacttgtc ttgccgttg caaaacgag atgagaaaa 
      ccgtcagat tttaaaaaa ccttttgga gttttaaat 
      gttgggatg tgtgttgtc accgttctt tacattcta 
      ataggaacc ctctcgtac ctgaaatac ggcgaagat 
      atagagggt agtgttact cttaatttg ccaaagggt 
      gatatcttg gcccagtct gtgaaaata atcatctcg 
      ctgggaatc ctcctgact tacgctctc cagttttac 
      atcgctgtt gagatcatg tttccaact cttgaaaga 
      atgttggga cctttcaag tatccagtt ttcgccgaa 
      ttatccttt agatctgtg ttagtttta ataacattt 
      attctggct gaagctatt ccatttttg aatcatttt 
      atttcgctg gttggagct gttagtagt gcgacatta 
      gcattgatc tttccgcca attctggat cttgttaca 
      agctacagt ttcggtgat ttgaagtgc acaacagtt 
      gtgaaaaat gtgataatt ttaatagtt ggagtcgtc 
      ggatgcatc acaggaact tatgagagt ataaattcg 
      attgttgac gctttcaac aacagtagc aaacaagaa 
      cactga
    mRNA sequence
    • >TC031063 RA
      tatctttaa agtcggctt acaaaggta tgtgccggg 
      tcagtagtg tgtgcattg caagcaaga tcagtaatt 
      tgttgtata actaactca ctttataca gtatggaaa 
      cgccaagca atgcaagtg ccccttcaa acaacgtca 
      agcagccat ggtacggct caaaccctg gctaccaat 
      cggacacaa aagataaaa gcaatgaaa atgtattta 
      tgacttttc agtctaaag acccaatca tttccatcc 
      ccttgcaag aaaagcacc ccaaagaca aagtcccgg 
      gaagcgggg gtggtcatg gacatggca tttccgtag 
      aacatccta cgtcatatg gagagacct tgatgcacc 
      ttttcaaag ggaatgttg gatcgggga tttttgcca 
      tgggagatg ccatccgga acgctggaa ttatcgttg 
      gacccggaa ttgtcctgc tgttagggg tcatttgtg 
      ttcattgcc aacacttat tgttatcag cggccctaa 
      aaatgaaat cgatgaaag aagtctccg tccctccgg 
      actttgccg aaacagtgg agctctgct tcgccaccg 
      gccctccag ccataaaaa aaatttcta aaattatga 
      aaattgttg taaatactt ttttgtgta taacccagt 
      tggggtttt gctgtgtct actttgttt ttatttcgg 
      aaaacgtca agaaggttt tggattatt atggctatg 
      agctggacg tccattttc acatggcaa taatccttc 
      tgccgatcc tttgtacgt ctctagttc gtaatttaa 
      aatatctcg caccgtttt ccacggttg cgaatattt 
      tcatgttga tgggcctta tcatcacag tttattaca 
      cgacacaag accttccgt ccttttcgg aacgaaatt 
      actacgcgg aaccgtcac aactgccgc ttttctttg 
      gaacagcag tgtttgcct tcgaaggaa tcggacttg 
      tcttgccgt tgcaaaacg agatgagaa aaccgtcag 
      attttaaaa aaccttttg gagttttaa atgttggga 
      tgtgtgttg tcaccgttc tttacattc taataggaa 
      ccctctcgt acctgaaat acggcgaag atatagagg 
      gtagtgtta ctcttaatt tgccaaagg gtgatatct 
      tggcccagt ctgtgaaaa taatcatct cgctgggaa 
      tcctcctga cttacgctc tccagtttt acatcgctg 
      ttgagatca tgtttccaa ctcttgaaa gaatgttgg 
      gacctttca agtatccag ttttcgccg aattatcct 
      ttagatctg tgttagttt taataacat ttattctgg 
      ctgaagcta ttccatttt tgaatcatt ttatttcgc 
      tggttggag ctgttagta gtgcgacat tagcattga 
      tctttccgc caattctgg atcttgtta caagctaca 
      gtttcggtg atttgaagt gcacaacag ttgtgaaaa 
      atgtgataa ttttaatag ttggagtcg tcggatgca 
      tcacaggaa cttatgaga gtataaatt cgattgttg 
      acgctttca acaacagta gcaaacaag aacactgat 
      ttttattca agactgtta gtcaataag tgacgttct 
      atcgggata gtggttgta ttttaatta ttaatttat 
      tatgtacat gtgtatcat agttcagat aagcatttt 
      accataagt gattctatg cgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY