Status
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Gene information
General information
TC004403 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19815300..19839077
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004403
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00689

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    gnathal appendages partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004403 RA
      MPPKKAASG AKTATKGKT AKKADSGNK EETQVEEAK 
      KPQKRKAVA SKKETEPDS TKAPKNSTV TNWENINFK 
      CTTKSPKGK APNFHITTW NVGGLRSWL KKGCLDILK 
      YDEPDILCI QETKCAVDK LPEEVKTIE GYETLWCSS 
      DKDGYAGVG IMVKDKPLN VIYGIDSKE HDTEGRCIT 
      VEYETFFVV NVYVPNAGR KLVTLPKRL DWNEQFEKF 
      IKNLDSQKP VIICGDMNV AHKEIDLAN PKTNTKNAG 
      FTVEERDGM TAFLGNGYV DIYRHLYPE KEKAYTFWT 
      YMGNARAKN VGWRLDYFL VSQRFVDNV CDNIIHSDV 
      LGFIAPNLE DRRYRITSL SYSHDGQDM LVSYSSDYL 
      YLFRIQENQ LAELKKPIK ASPWEKVKM LAGQRERVN 
      NSPPHVRRL RLRGDWSDT GPDARPERD TTTTVSIGQ 
      ARPQLHATL MQRMTDVLS QMLNDPMTR AALSAGGED 
      SLDPNETSQ RNILGERQS NSSAPEENA EQPVEQSQN 
      EAEGEQSVQ TEAEEVATT SRQDQEPSA STSNAEERQ 
      SETSACVTS LYSHLTTLR NLRDGFIEQ HGSEPSVSL 
      RYSQQSTAN ATISLRVGD EFNRGNFDN SQNDASAVP 
      STSQSEHTS MRSPSGLAD DGASSDAEF DDYDDEYPD 
      SSLPQKTDT VNTYEAEMK YKYVGHRNA RTMIKEATF 
      WGNDYVMSG SDCGHVFIW DKNTTKLKM LLQADQHVV 
      NCLQPHPTL PLLATSGID HDVKLWAPI LEEPGFNVK 
      MAEDLINRN AIMLEETRD TITVPAAFM IRMLACLNQ 
      IRRGARARS RRQDEEEEN N
    CDS sequence
    • >TC004403 RA
      atgccaccc aaaaaagct gcatctggt gccaagaca 
      gccaccaag ggtaaaact gccaagaag gcagacagt 
      ggaaataaa gaagaaacc caagtggaa gaagcgaaa 
      aaacctcaa aaacgcaaa gctgttgct agtaagaaa 
      gagactgag ccagatagt acaaaggcg cctaaaaat 
      tcgacagta actaattgg gaaaatata aatttcaaa 
      tgcactacg aaatccccg aaaggaaaa gctcccaac 
      tttcatatc actacgtgg aatgtaggc ggattaaga 
      agctggtta aagaaggga tgccttgat atcctgaag 
      tatgacgaa ccagatata ctttgtatt caagagact 
      aagtgcgct gtagataaa ttaccagaa gaagtcaag 
      actatagaa ggttatgaa acattgtgg tgtagtagc 
      gataaagat ggctatgca ggtgtaggc attatggtt 
      aaagataaa ccacttaat gttatttat gggattgac 
      tctaaagaa catgataca gagggcaga tgtatcact 
      gtggagtat gaaactttc tttgtagtc aacgtatat 
      gtccccaat gcaggtcga aaactagtc accttgcca 
      aaacgttta gattggaac gaacaattc gagaagttt 
      attaaaaat ttggactcc caaaaacca gtcataatt 
      tgtggggat atgaacgtg gcacacaaa gagattgac 
      ttggccaat cccaaaacc aacaccaaa aatgctggg 
      tttacagtg gaggaacgt gacggcatg actgctttt 
      cttggaaac ggttatgtt gatatttat cgccactta 
      tatcccgag aaggaaaaa gcttacact ttctggacc 
      tacatggga aatgccaga gctaagaac gttggatgg 
      cgcttggat tatttctta gtctcccaa agatttgtg 
      gacaacgtt tgcgataac ataatacat agtgatgtt 
      ctgggtttc atcgcccca aatctggaa gaccgaaga 
      tacagaata acttcactc agctacagt catgacggt 
      caagatatg ctagtcagt tactcatct gattatctg 
      tatctattt aggatacag gagaatcag ctagctgaa 
      ttaaaaaaa cccattaaa gcgtctcct tgggagaaa 
      gttaaaatg ttagcaggc caacgagaa cgtgtcaat 
      aattcgcca cctcatgtg cgaagatta cgtttaaga 
      ggagattgg tctgatact ggaccagat gcacgtcca 
      gaaagagac acaacgaca actgtcagt attggtcaa 
      gcgagacca caattacac gccactttg atgcagcgt 
      atgaccgat gttctctca caaatgttg aatgatcca 
      atgacaaga gcggctttg agtgcagga ggagaggat 
      tcacttgat ccgaatgaa acgtcgcaa agaaatatt 
      ttaggtgaa agacaaagt aattcgtct gctccggaa 
      gagaacgca gaacagccg gtggaacag agtcagaat 
      gaagcggaa ggagagcaa agtgttcaa acagaggca 
      gaggaagtt gccacaaca agcaggcaa gaccaagag 
      ccgtctgcg tccacaagt aacgctgaa gaaagacaa 
      agtgaaact tctgcatgc gttacaagt ctgtacagt 
      cacctaaca acgttgcgt aacttaagg gacggcttt 
      atagaacaa catggttct gaaccttca gtcagcctc 
      aggtattct cagcaaagc acagccaat gctacgatc 
      agtttaaga gttggtgat gaatttaat cgtggaaat 
      ttcgataat tcacaaaat gatgcctca gcggtgcct 
      tcaacgtca caaagtgaa catacatcg atgagaagt 
      ccaagtgga ctggctgat gacggcgca agctctgat 
      gccgagttc gatgattat gacgatgaa tatcctgat 
      tcgtcgtta ccacaaaag actgatacc gtaaataca 
      tacgaagcg gaaatgaaa tataagtac gtaggacac 
      agaaatgct cgaactatg ataaaagaa gctacattt 
      tggggcaat gactacgtg atgtcgggc tcagactgt 
      ggtcacgta tttatttgg gacaaaaac accacaaaa 
      ttaaaaatg ttgctacaa gctgatcaa catgtggtc 
      aattgttta caacctcac cctactttg ccacttctt 
      gccactagt ggaatagat catgatgtt aaattatgg 
      gctcctatt ttagaagag ccaggtttt aacgtcaag 
      atggctgaa gatctgatc aaccgtaat gcaatcatg 
      ttggaagaa acccgagat accataaca gtacctgca 
      gccttcatg ataagaatg ttagcttgt ttaaatcaa 
      attcgccga ggcgctaga gctcggtct agacgtcag 
      gatgaagaa gaagaaaac aattaa
    mRNA sequence
    • >TC004403 RA
      gcagcgctt tttttcgtc tgtcaaaaa ctcggcgtt 
      ttttggcgc acattgaaa taccgttga attttgtgt 
      tagttgtga taaaattgc aaaatgcca cccaaaaaa 
      gctgcatct ggtgccaag acagccacc aagggtaaa 
      actgccaag aaggcagac agtggaaat aaagaagaa 
      acccaagtg gaagaagcg aaaaaacct caaaaacgc 
      aaagctgtt gctagtaag aaagagact gagccagat 
      agtacaaag gcgcctaaa aattcgaca gtaactaat 
      tgggaaaat ataaatttc aaatgcact acgaaatcc 
      ccgaaagga aaagctccc aactttcat atcactacg 
      tggaatgta ggcggatta agaagctgg ttaaagaag 
      ggatgcctt gatatcctg aagtatgac gaaccagat 
      atactttgt attcaagag actaagtgc gctgtagat 
      aaattacca gaagaagtc aagactata gaaggttat 
      gaaacattg tggtgtagt agcgataaa gatggctat 
      gcaggtgta ggcattatg gttaaagat aaaccactt 
      aatgttatt tatgggatt gactctaaa gaacatgat 
      acagagggc agatgtatc actgtggag tatgaaact 
      ttctttgta gtcaacgta tatgtcccc aatgcaggt 
      cgaaaacta gtcaccttg ccaaaacgt ttagattgg 
      aacgaacaa ttcgagaag tttattaaa aatttggac 
      tcccaaaaa ccagtcata atttgtggg gatatgaac 
      gtggcacac aaagagatt gacttggcc aatcccaaa 
      accaacacc aaaaatgct gggtttaca gtggaggaa 
      cgtgacggc atgactgct tttcttgga aacggttat 
      gttgatatt tatcgccac ttatatccc gagaaggaa 
      aaagcttac actttctgg acctacatg ggaaatgcc 
      agagctaag aacgttgga tggcgcttg gattatttc 
      ttagtctcc caaagattt gtggacaac gtttgcgat 
      aacataata catagtgat gttctgggt ttcatcgcc 
      ccaaatctg gaagaccga agatacaga ataacttca 
      ctcagctac agtcatgac ggtcaagat atgctagtc 
      agttactca tctgattat ctgtatcta tttaggata 
      caggagaat cagctagct gaattaaaa aaacccatt 
      aaagcgtct ccttgggag aaagttaaa atgttagca 
      ggccaacga gaacgtgtc aataattcg ccacctcat 
      gtgcgaaga ttacgttta agaggagat tggtctgat 
      actggacca gatgcacgt ccagaaaga gacacaacg 
      acaactgtc agtattggt caagcgaga ccacaatta 
      cacgccact ttgatgcag cgtatgacc gatgttctc 
      tcacaaatg ttgaatgat ccaatgaca agagcggct 
      ttgagtgca ggaggagag gattcactt gatccgaat 
      gaaacgtcg caaagaaat attttaggt gaaagacaa 
      agtaattcg tctgctccg gaagagaac gcagaacag 
      ccggtggaa cagagtcag aatgaagcg gaaggagag 
      caaagtgtt caaacagag gcagaggaa gttgccaca 
      acaagcagg caagaccaa gagccgtct gcgtccaca 
      agtaacgct gaagaaaga caaagtgaa acttctgca 
      tgcgttaca agtctgtac agtcaccta acaacgttg 
      cgtaactta agggacggc tttatagaa caacatggt 
      tctgaacct tcagtcagc ctcaggtat tctcagcaa 
      agcacagcc aatgctacg atcagttta agagttggt 
      gatgaattt aatcgtgga aatttcgat aattcacaa 
      aatgatgcc tcagcggtg ccttcaacg tcacaaagt 
      gaacataca tcgatgaga agtccaagt ggactggct 
      gatgacggc gcaagctct gatgccgag ttcgatgat 
      tatgacgat gaatatcct gattcgtcg ttaccacaa 
      aagactgat accgtaaat acatacgaa gcggaaatg 
      aaatataag tacgtagga cacagaaat gctcgaact 
      atgataaaa gaagctaca ttttggggc aatgactac 
      gtgatgtcg ggctcagac tgtggtcac gtatttatt 
      tgggacaaa aacaccaca aaattaaaa atgttgcta 
      caagctgat caacatgtg gtcaattgt ttacaacct 
      caccctact ttgccactt cttgccact agtggaata 
      gatcatgat gttaaatta tgggctcct attttagaa 
      gagccaggt tttaacgtc aagatggct gaagatctg 
      atcaaccgt aatgcaatc atgttggaa gaaacccga 
      gataccata acagtacct gcagccttc atgataaga 
      atgttagct tgtttaaat caaattcgc cgaggcgct 
      agagctcgg tctagacgt caggatgaa gaagaagaa 
      aacaattaa atttcaacg tatgtataa atacttacc 
      atgtttgtg ggaagtaac ataggtaga aatcaaatt 
      cacgatgta catacttgc ttatgttaa ctttaccgt 
      aaattacag gattgttgg atttcttca ttatgtcgt 
      aactacatg atcaattgt gttttgaca attaatttt 
      ttccttggt taaagttcc gagttctag cgtttggtc 
      taatataat tatttgatg tatttatta ttttcatag 
      ttcatatta aattattga aaccttgaa ctcgtttta 
      tcatttttt aataaaaca acaaataca aacgaaaca 
      tttattttt acacaatat tcaacaatt ccaaagata 
      actaaatct agtatcacc caaaatatt tacaacaga 
      aagttacat gcatttgta tgtatgtac acacattta 
      attggaaag tcatacgta tatatttct atactaaac 
      ttagacttc ctttttggc aaaaacgat cactttggt 
      tctatgttt agtggcttg ttttttcta taaaatcaa 
      agtcggaaa ttatataaa gtatgctac aaaaatgac 
      acatgcatg cgtatgtga agtggaatc cctttttta 
      taaatagaa ttgacttta agagaaaaa aattatttt 
      atgttggca aaaaaggga ttttttgct atctacatt 
      cgtatatgc agcaggcaa acagccaat ttgttagta 
      catgcaagt acagctttt tttcaaatt gttgcggtc 
      agatggcgt catagagta aaacttctg ataaacaaa 
      tatatcata aatacaaat tcataccct acctactat 
      tttacttga atctaaaaa aagaaaatt aagtatact 
      ttcgtttcc actaaacca tatcacaat gacaacaaa 
      cttgactat gactgtaat ttgtcatta atgtaaaaa 
      tatgaacaa tcaaaattg gcaaattca tgcagtggt 
      aggaaaact tctcttcac caacttttt aatcgtatt 
      tttaacgtg tgcagatca taacatttg agctgtttt 
      acataagat cgtttttac tctttgggg agttttcta 
      cttaaacta tttgcgtct gtgagaagc aaactacca 
      tagaatgtg atcaatttg caaaaataa tctttgtcg 
      aatttttta ggtacgaag ttcaggttc attttcaaa 
      ttgtataat aattttaag tggtattta aaagaactg 
      ttgtgttgt ataaaacac tacaaaacc gtaattttt 
      cgaagtagc ttcccttaa aaatataat ttttgtata 
      atgtacaga aaaaaaaat agaaagaac gagtattaa 
      ctagtgaca ggtaactga cgaaaaacc attcatact 
      gaatctcac ttatttaga agtaattta aataaacta 
      tatttaatg ct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY