Status
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Gene information
General information
TC004391 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 7109933..7114787
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004391
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00688

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004391 RA
      MACPKAKSR KAEPDSTTK EADFGPGQD VLLKQKDGR 
      YYLGTVVEV DGARERCLV KFGDNTDSW SSFKDLTKL 
      SQPQQEDLL CVVCKKSTP KNNTEITVC DKCGRGYHR 
      KCHQPEIPN ECQKEDSVW MCKRCTSSE PHRLKKTDS 
      KHLKRESRN AASSSSDSR FIAPITKVL PYDLNSLTW 
      DTYHRVNDE HIYCYCGEN GEWYKQMLQ CTRCKQWFH 
      ERCLDCLQY PLYCGDRFY VFVCSICNH GKEFLRRLE 
      MKWVDLVHL MLFNLTAYN SKKYYDLDT TVIPYINDN 
      WHALQLPPK IVSVSKSER RDNILSVLT NNRNRFKCG 
      REIKKRTTI WGLRVRLPP PAPTVTLNF NGCITEEEL 
      REVWQGNRR LQFLPFAEN GGVVVPCDN HMRGLMMGV 
      SYQQNGNRS ETDSPCPSP EREEPLHLL AGCAKKTVP 
      FSNITGQRR GKRRNTTTN DETERYLTR FSLDSSKNY 
      KSESLPLTP PTSVSAPPT PPASGSNSL LSDDFSEPL 
      LKKGGGESS GDETSSKST LDLIIPPPK DFEGKNNPF 
      LALLRGGDE PKKRRSKDT ITLPLPLTA VIPGKPLMR 
      PKKRQLSEK DIFIGPNGE VKRRRLRRS RFEKTASKT 
      ATVLPVRTD SKEWGQRGT EFALNGRRL RQRPEKAVP 
      EKEKAGPTT KPSPVKQEP EINMDDLKS SVNIYFGAA 
      NRIAAGERF AVKAKRVGP SGKLEFLIE WEGPGNGMT
    CDS sequence
    • >TC004391 RA
      atggcgtgc cccaaggcg aagagccgc aaggcggag 
      cccgactcc accaccaag gaggccgac tttgggccc 
      ggtcaggac gtgctgctc aagcaaaag gacggcagg 
      tactatctg ggaacggtg gtcgaagtt gatggggcc 
      agggaacgg tgtctcgtc aaattcggg gacaatacg 
      gacagttgg tcgagtttc aaggatttg accaagttg 
      agtcaaccg caacaggag gatctcttg tgtgtcgtt 
      tgcaagaaa tccacgccc aaaaacaac accgaaatc 
      acggtctgt gataaatgc ggcaggggg taccatcgc 
      aagtgtcat cagcccgaa attcccaac gaatgccaa 
      aaagaagat tcagtatgg atgtgtaaa agatgtaca 
      agcagcgaa ccacataga ctgaaaaaa actgattca 
      aagcatttg aagcgagaa tctcgaaat gcggcttcg 
      tcaagtagc gacagtcgt tttattgct ccaattaca 
      aaagtatta ccttacgat ttaaattcg cttacatgg 
      gacacatat catcgggta aacgacgaa cacatttat 
      tgttattgt ggtgaaaat ggcgaatgg tacaaacaa 
      atgttacaa tgtacaagg tgtaaacaa tggtttcat 
      gaaaggtgt ttggattgt cttcagtat ccgttatat 
      tgcggcgac aggttttat gtgtttgtt tgttcgatt 
      tgtaatcac ggcaaggag tttttaaga cgtttggaa 
      atgaaatgg gttgatctt gttcacttg atgcttttc 
      aatctcaca gcttacaat agtaaaaag tattacgat 
      ttggacaca actgtcatt ccttatatt aatgacaat 
      tggcatgcc ttacagcta ccaccaaag atcgtttca 
      gtgagtaaa tcagaacgt cgtgacaac atcctgtca 
      gttctcaca aacaatcga aatcgtttc aagtgcggt 
      cgtgaaata aaaaaacgc accacaata tgggggctg 
      cgtgtgcgt ttaccgccg cccgccccc accgtaacc 
      ctaaatttc aatgggtgt atcacggag gaggagcta 
      cgtgaggtg tggcaaggt aatcgccgc ctacaattc 
      ctcccattt gctgaaaat ggaggcgtg gtcgtgccg 
      tgtgataat catatgcgt ggtcttatg atgggcgtg 
      tcctatcag caaaacggt aatcgttct gaaaccgat 
      tcgccttgt cccagcccc gaaagggag gagccattg 
      catttattg gccggttgt gcgaaaaaa accgtccca 
      ttttcgaat attacgggt caaaggcgt ggcaaacga 
      cgcaatacg acgaccaat gacgaaacc gaacgctat 
      ttaacacgt ttcagtttg gatagcagt aagaattat 
      aaaagcgaa tcactgcct ttgactccg cctacttct 
      gtttcggca ccgcctaca ccgcccgcc tccggttcg 
      aattcactt ctatcggat gatttttcc gagccattg 
      ttgaaaaaa ggcggcggt gaatcatca ggtgatgaa 
      acgtcatca aaaagcaca cttgattta ataataccg 
      ccaccgaaa gatttcgaa ggtaaaaat aatccattt 
      ttggcgtta ctaaggggc ggggatgag ccgaaaaaa 
      aggcggagc aaagataca attacttta cctctacct 
      ttaacggct gtgatacca ggcaaacca ttaatgcga 
      ccgaagaaa cgccaattg agcgaaaaa gatattttt 
      attggacca aatggtgaa gttaaaagg cggcgatta 
      aggcggagc cgatttgag aaaacagcc agtaaaaca 
      gcaacggtt ctgccggtc agaactgat tcaaaggaa 
      tggggacaa cgagggacg gagtttgca ttaaatggg 
      cggaggttg cgacagcgg cccgagaag gcggtgcca 
      gagaaggaa aaggcgggg ccaacaaca aaaccgagt 
      ccagtcaaa caagagccg gagataaac atggacgat 
      ttgaagagt tcagtaaat atttatttt ggggcggcg 
      aatcggatc gcagcaggc gagaggttt gcggttaag 
      gcgaaaagg gtgggtccg agtggaaaa ctggagttt 
      ttgatcgag tgggagggg cctggcaac ggaatgact 
      tga
    mRNA sequence
    • >TC004391 RA
      gtagtattg tagttttgt gtttgtgtt cgcggcgcc 
      ctctgttct tcctccaag tgtcatcgt gtgaaaatg 
      tcgttaatt ttgaacaaa atcgcagaa aatttcgtt 
      taaattgaa taaagtgtg tcgttttgt tcgtttgtc 
      gtgcttgtg cacacattt cgtgtgcct tttgtgtgc 
      tttttgctg cgtaaagtg cgtcagatt cgtgccgat 
      ccgtttggc gcgctttga ccaaacaag agtcgtttt 
      aatggccgc aaaagcacc actcaattt ggatggacg 
      ttgaggggt tgtgggcag ggggcgcat gtcttgggc 
      tgtgatggc gtgccccaa ggcgaagag ccgcaaggc 
      ggagcccga ctccaccac caaggaggc cgactttgg 
      gcccggtca ggacgtgct gctcaagca aaaggacgg 
      caggtacta tctgggaac ggtggtcga agttgatgg 
      ggccaggga acggtgtct cgtcaaatt cggggacaa 
      tacggacag ttggtcgag tttcaagga tttgaccaa 
      gttgagtca accgcaaca ggaggatct cttgtgtgt 
      cgtttgcaa gaaatccac gcccaaaaa caacaccga 
      aatcacggt ctgtgataa atgcggcag ggggtacca 
      tcgcaagtg tcatcagcc cgaaattcc caacgaatg 
      ccaaaaaga agattcagt atggatgtg taaaagatg 
      tacaagcag cgaaccaca tagactgaa aaaaactga 
      ttcaaagca tttgaagcg agaatctcg aaatgcggc 
      ttcgtcaag tagcgacag tcgttttat tgctccaat 
      tacaaaagt attacctta cgatttaaa ttcgcttac 
      atgggacac atatcatcg ggtaaacga cgaacacat 
      ttattgtta ttgtggtga aaatggcga atggtacaa 
      acaaatgtt acaatgtac aaggtgtaa acaatggtt 
      tcatgaaag gtgtttgga ttgtcttca gtatccgtt 
      atattgcgg cgacaggtt ttatgtgtt tgtttgttc 
      gatttgtaa tcacggcaa ggagttttt aagacgttt 
      ggaaatgaa atgggttga tcttgttca cttgatgct 
      tttcaatct cacagctta caatagtaa aaagtatta 
      cgatttgga cacaactgt cattcctta tattaatga 
      caattggca tgccttaca gctaccacc aaagatcgt 
      ttcagtgag taaatcaga acgtcgtga caacatcct 
      gtcagttct cacaaacaa tcgaaatcg tttcaagtg 
      cggtcgtga aataaaaaa acgcaccac aatatgggg 
      gctgcgtgt gcgtttacc gccgcccgc ccccaccgt 
      aaccctaaa tttcaatgg gtgtatcac ggaggagga 
      gctacgtga ggtgtggca aggtaatcg ccgcctaca 
      attcctccc atttgctga aaatggagg cgtggtcgt 
      gccgtgtga taatcatat gcgtggtct tatgatggg 
      cgtgtccta tcagcaaaa cggtaatcg ttctgaaac 
      cgattcgcc ttgtcccag ccccgaaag ggaggagcc 
      attgcattt attggccgg ttgtgcgaa aaaaaccgt 
      cccattttc gaatattac gggtcaaag gcgtggcaa 
      acgacgcaa tacgacgac caatgacga aaccgaacg 
      ctatttaac acgtttcag tttggatag cagtaagaa 
      ttataaaag cgaatcact gcctttgac tccgcctac 
      ttctgtttc ggcaccgcc tacaccgcc cgcctccgg 
      ttcgaattc acttctatc ggatgattt ttccgagcc 
      attgttgaa aaaaggcgg cggtgaatc atcaggtga 
      tgaaacgtc atcaaaaag cacacttga tttaataat 
      accgccacc gaaagattt cgaaggtaa aaataatcc 
      atttttggc gttactaag gggcgggga tgagccgaa 
      aaaaaggcg gagcaaaga tacaattac tttacctct 
      acctttaac ggctgtgat accaggcaa accattaat 
      gcgaccgaa gaaacgcca attgagcga aaaagatat 
      ttttattgg accaaatgg tgaagttaa aaggcggcg 
      attaaggcg gagccgatt tgagaaaac agccagtaa 
      aacagcaac ggttctgcc ggtcagaac tgattcaaa 
      ggaatgggg acaacgagg gacggagtt tgcattaaa 
      tgggcggag gttgcgaca gcggcccga gaaggcggt 
      gccagagaa ggaaaaggc ggggccaac aacaaaacc 
      gagtccagt caaacaaga gccggagat aaacatgga 
      cgatttgaa gagttcagt aaatattta ttttggggc 
      ggcgaatcg gatcgcagc aggcgagag gtttgcggt 
      taaggcgaa aagggtggg tccgagtgg aaaactgga 
      gtttttgat cgagtggga ggggcctgg caacggaat 
      gacttgatt gatgaccaa tcaaattgt gttcatgtg 
      accattaag aggcggtgc tttgtaaat aaaattgta 
      gagtgtatg tgtgtgtgt gtgttgatg gtcacagat 
      ttattattt ttttggaca gttccttgc acgcgaaat 
      agttggact acatcatta cttgacttg aaaacttcc 
      gatttgcca aagaggccg attgttttt tcgctaatc 
      aaataactg ccaatgttg agtcactgc caattgttt 
      tttttttaa tttttgtgt gttaaacat gtaatccaa 
      ctgaagttg tgatataaa acgagaaaa agttaggga 
      attttttat ttggaattg taataaatg gccttgaca 
      tttctatac aggtgcatt cagtaatta ttagttggg 
      acattaagt ttgtcaccc cctttatat ttttttgtc 
      tttttattt ctatcttaa gtatgtagt taatgagac 
      tgaaattat ttttactag tttttattc acacttgac 
      gtttgtgaa acgtcattt tcagtcaaa atttgtatt 
      tttattaac aattattgt taaataaag caacttgta 
      ttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY