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Gene information
General information
TC004390 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 7107623..7109652
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004390
    iBeetle screen iB_00687

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004390 RA
      MMDNSKGGN SDEEYASKS KQSNVLPLW GNERTMNLN 
PLILTNIQS SHYFKVNLY ELKTYHEVV DEIYYKVNH 
LEPWEKGSR RTSGQTGMC GGVRGVGAG GIVSTAYCL 
LYKLFTLKL TRKQLNGLI NHCDSPYIR ALGFMYIRY 
TLPPKDLLE WYEDYLEDE EELDVKAGG GQMMTIGTM 
LRQWLVKLE WFSTLFPRI PVPIQQKIQ KHLEVRFPP 
YAIKAAAAA AAAAAAAVA TATTNERPR NTERERNDW 
SRDRDRHSV REDIRKDFL RDNERERRR DRYRDDRRH 
EKKIRERSR ERDRDRDRS PHRGDRDRE HSKYRKGHH 
R
    CDS sequence
    • >TC004390 RA
      atgatggac aacagcaag ggtgggaac agcgatgaa 
gaatatgcg tccaaatcc aagcaaagt aacgtattg 
ccactttgg ggcaacgaa cgcaccatg aatttgaat 
cctttgatt ttgacaaac atacaaagt tcgcattat 
ttcaaagtc aatctttac gaattgaag acgtaccac 
gaagtcgtc gatgaaatc tactacaaa gttaatcat 
ttggagccc tgggagaag ggttcgcgt cgcacttcg 
ggtcaaacg ggtatgtgc ggaggggtg agaggtgtg 
ggggccgga ggcattgtc tccaccgcc tactgtctc 
ctctacaaa ctgttcacg ctaaaactg acccgtaaa 
caactaaac ggcctcata aaccactgc gattcgccc 
tatattaga gcgttaggt ttcatgtac attagatac 
actttgccc ccaaaagat ctcctggaa tggtacgaa 
gattactta gaagatgaa gaggaattg gatgttaaa 
gcaggaggg gggcaaatg atgaccatt gggacaatg 
ctgaggcaa tggttggtc aaattggag tggttttcg 
acgctattt ccacgaatt ccggtgcct atacagcag 
aaaattcaa aaacatttg gaagttaga ttccctcca 
tatgctatc aaagcagcc gctgctgct gctgctgcc 
gccgcagca gctgttgcc acggcaaca accaatgag 
agaccgaga aacacggag agggaacgt aatgattgg 
tcgcgtgac agagacaga catagtgtt agagaagat 
ataagaaag gattttttg cgcgataat gagagagaa 
cgaagaaga gacagatac agggatgac cgacgacat 
gagaagaaa atcagggaa agaagtcgg gaacgtgac 
agagacaga gataggagt ccccatagg ggagacaga 
gacagggaa cacagcaaa taccgaaaa ggacaccat 
agataa
    mRNA sequence
    • >TC004390 RA
      gaataaatc aatacaaaa cgtaattat tacaatcac 
atgtcgact ggggcgttt gaaacctca gccagatcc 
tgttttgct tttaatttt tcgcgaatt aactcagcc 
atggccttt tgtgtccgt ttggctaac ttttcgagt 
tttttctca acgtcacgt ttcaaatcc cagtcgggt 
tgtctcgga gcaagtgac gaaatgtcc aattgatca 
ataaccatc tcaccttta gccaaatcg agcaaatcc 
ttgacttcg ttccgcaca tcaattgat tcacgtttt 
gtgactaaa tgctcattc aaattctta tcttgtggc 
ctgtaactg cgaaatttc ggcctgaaa acacaaata 
agcacacga aaaatcgcc tttagggct gttttacgt 
tggtagagt ttgtgcggc gtcttggtc acttttcgt 
atcgttgga cttgtcttc acgtttgcg tttaagtga 
ttttaagcg ttcctgtct tttgcgtgc ttgttcctc 
caaactgtt cacttcctc gctcatttt ttcgctttt 
tgagatcgt cataacccg acttttttg gggattttg 
gtgggacgg ttagtgcga gttggtgat taaatggcg 
gtgttgtgt caagtaatt gggaaaaaa attttgatt 
tttgggtaa tttgacgat ttttgtgac acaaattgc 
gaaatgatg gacaacagc aagggtggg aacagcgat 
gaagaatat gcgtccaaa tccaagcaa agtaacgta 
ttgccactt tggggcaac gaacgcacc atgaatttg 
aatcctttg attttgaca aacatacaa agttcgcat 
tatttcaaa gtcaatctt tacgaattg aagacgtac 
cacgaagtc gtcgatgaa atctactac aaagttaat 
catttggag ccctgggag aagggttcg cgtcgcact 
tcgggtcaa acgggtatg tgcggaggg gtgagaggt 
gtgggggcc ggaggcatt gtctccacc gcctactgt 
ctcctctac aaactgttc acgctaaaa ctgacccgt 
aaacaacta aacggcctc ataaaccac tgcgattcg 
ccctatatt agagcgtta ggtttcatg tacattaga 
tacactttg cccccaaaa gatctcctg gaatggtac 
gaagattac ttagaagat gaagaggaa ttggatgtt 
aaagcagga ggggggcaa atgatgacc attgggaca 
atgctgagg caatggttg gtcaaattg gagtggttt 
tcgacgcta tttccacga attccggtg cctatacag 
cagaaaatt caaaaacat ttggaagtt agattccct 
ccatatgct atcaaagca gccgctgct gctgctgct 
gccgccgca gcagctgtt gccacggca acaaccaat 
gagagaccg agaaacacg gagagggaa cgtaatgat 
tggtcgcgt gacagagac agacatagt gttagagaa 
gatataaga aaggatttt ttgcgcgat aatgagaga 
gaacgaaga agagacaga tacagggat gaccgacga 
catgagaag aaaatcagg gaaagaagt cgggaacgt 
gacagagac agagatagg agtccccat aggggagac 
agagacagg gaacacagc aaataccga aaaggacac 
catagataa atttgtttc actttctca cgttagaat 
tacacttaa gtacacttt acagaattt attttttgt 
tttgagttt tttaataaa gtctttttt cctaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY