Status
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Gene information
General information
TC004378 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6946043..6947867
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004378
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00686

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004378 RA
      MDNLEANIM DKSMRSVFV GNIPYEATE EKLKDIFGE 
VGQVLSFKL VFDRETGKP KGYGFCEYR DQETALSAM 
RNLNGYEIG GRNLRVDNA CTEKSRMEM QNLLNQPPV 
ENPYGEPIQ AEKAPEAIS KAVASLPPE QMFELMKQM 
KLCIQNNPT EARQMLLQN PQLAYALLQ AQVVMRIID 
PHTAATMLH PQNPVPSSL TPKEKTNPG PIPINSNYV 
SNPTPVQPP RQDFQPVPA PAIHNNSNP VFTGQDVDL 
RSLDPRGAD PRLSRLGDQ DMRTLPGQL PNPLPPPLV 
ENFNRDPRG GPANFPSDP RQQQRADPR QKNMPPQQN 
AMPPQPVRA PQGPPSTPQ VRQAANVAS SIGTAGASD 
QEKAALIMQ VLQLSDEQI AMLPPEQRN SILVLKEQI 
AKSAHR
    CDS sequence
    • >TC004378 RA
      atggataat ttagaagca aatatcatg gacaagtca 
atgcgttcg gtctttgtg ggcaacatc ccctacgaa 
gccaccgaa gagaagctc aaagacatt ttcggcgaa 
gtaggccag gtcctgtca ttcaagttg gttttcgac 
cgcgagaca ggcaagccc aaaggctac ggcttctgc 
gagtaccgc gaccaggag acggcgctc agcgccatg 
cgcaacctc aacgggtac gagatcggg gggcgcaac 
ctccgggtc gacaacgcc tgtaccgag aaatcgcgc 
atggagatg caaaacttg ctgaaccag cccccggtc 
gagaacccc tacggcgag ccgattcag gccgagaag 
gcccccgag gccatcagc aaagccgtg gcctcgttg 
ccccccgag cagatgttc gagttgatg aagcagatg 
aagctctgc attcagaac aaccccact gaggccagg 
cagatgctg ctgcagaac ccccagttg gcgtacgct 
ctgctgcag gcgcaggtg gttatgagg attattgac 
ccgcatacg gcggctaca atgttgcat ccgcaaaat 
cccgttcct tcgtctttg acgcccaag gaaaagact 
aatccaggc cccattccg attaatagt aattatgtc 
tcgaatccc acgcctgtg cagccccca agacaggac 
tttcagccg gtgccagcc cccgcgatt cataataat 
tccaatccg gtcttcaca ggtcaagac gttgaccta 
agatcactg gacccccga ggggctgat ccccgcctc 
agccgcctg ggcgaccaa gacatgcgc actttgccc 
ggacaactc cccaacccc cttccgccg ccacttgtc 
gaaaacttt aatagggac ccacgtgga ggtccggcc 
aacttccca tctgatccg agacaacaa cagcgagct 
gacccaaga cagaaaaat atgccccct cagcaaaac 
gcgatgcct ccgcagccg gtcagagcc ccgcagggg 
ccccccagt accccccaa gttcgccaa gctgccaat 
gttgcttca agtataggt acagctggg gcctcagac 
caggaaaag gcggccctg attatgcaa gtcctgcaa 
ttgtcagat gagcagatt gcaatgctg ccacctgaa 
cagcgtaat agtattcta gttttgaag gaacaaatc 
gccaaaagt gcacaccgt tga
    mRNA sequence
    • >TC004378 RA
      tacgattca ctttagggt tgtaaccac aatagataa 
accaacatt tttgttatt gttggcttt aaaatgcat 
aattttgta cttttttaa aaggtagag ggttggtaa 
cgctgaccg tttgttgaa attggtgtc ccaggtgtc 
ttgattact tcgttccaa gttggaatt aaacaatca 
ccatggata atttagaag caaatatca tggacaagt 
caatgcgtt cggtctttg tgggcaaca tcccctacg 
aagccaccg aagagaagc tcaaagaca ttttcggcg 
aagtaggcc aggtcctgt cattcaagt tggttttcg 
accgcgaga caggcaagc ccaaaggct acggcttct 
gcgagtacc gcgaccagg agacggcgc tcagcgcca 
tgcgcaacc tcaacgggt acgagatcg gggggcgca 
acctccggg tcgacaacg cctgtaccg agaaatcgc 
gcatggaga tgcaaaact tgctgaacc agcccccgg 
tcgagaacc cctacggcg agccgattc aggccgaga 
aggcccccg aggccatca gcaaagccg tggcctcgt 
tgccccccg agcagatgt tcgagttga tgaagcaga 
tgaagctct gcattcaga acaacccca ctgaggcca 
ggcagatgc tgctgcaga acccccagt tggcgtacg 
ctctgctgc aggcgcagg tggttatga ggattattg 
acccgcata cggcggcta caatgttgc atccgcaaa 
atcccgttc cttcgtctt tgacgccca aggaaaaga 
ctaatccag gccccattc cgattaata gtaattatg 
tctcgaatc ccacgcctg tgcagcccc caagacagg 
actttcagc cggtgccag cccccgcga ttcataata 
attccaatc cggtcttca caggtcaag acgttgacc 
taagatcac tggaccccc gaggggctg atccccgcc 
tcagccgcc tgggcgacc aagacatgc gcactttgc 
ccggacaac tccccaacc cccttccgc cgccacttg 
tcgaaaact ttaataggg acccacgtg gaggtccgg 
ccaacttcc catctgatc cgagacaac aacagcgag 
ctgacccaa gacagaaaa atatgcccc ctcagcaaa 
acgcgatgc ctccgcagc cggtcagag ccccgcagg 
ggcccccca gtacccccc aagttcgcc aagctgcca 
atgttgctt caagtatag gtacagctg gggcctcag 
accaggaaa aggcggccc tgattatgc aagtcctgc 
aattgtcag atgagcaga ttgcaatgc tgccacctg 
aacagcgta atagtattc tagttttga aggaacaaa 
tcgccaaaa gtgcacacc gttgattaa ttaagtcat 
ttccccaaa tcagtttaa tttttacag tttacatga 
taa

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY