Status
Please wait ...
Gene information
General information
TC004378 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid77: 33124..34948
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004378
    iBeetle screen iB_00686
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004378 RA
      MDNLEANIM DKSMRSVFV GNIPYEATE EKLKDIFGE 
      VGQVLSFKL VFDRETGKP KGYGFCEYR DQETALSAM 
      RNLNGYEIG GRNLRVDNA CTEKSRMEM QNLLNQPPV 
      ENPYGEPIQ AEKAPEAIS KAVASLPPE QMFELMKQM 
      KLCIQNNPT EARQMLLQN PQLAYALLQ AQVVMRIID 
      PHTAATMLH PQNPVPSSL TPKEKTNPG PIPINSNYV 
      SNPTPVQPP RQDFQPVPA PAIHNNSNP VFTGQDVDL 
      RSLDPRGAD PRLSRLGDQ DMRTLPGQL PNPLPPPLV 
      ENFNRDPRG GPANFPSDP RQQQRADPR QKNMPPQQN 
      AMPPQPVRA PQGPPSTPQ VRQAANVAS SIGTAGASD 
      QEKAALIMQ VLQLSDEQI AMLPPEQRN SILVLKEQI 
      AKSAHR
    CDS sequence
    • >TC004378 RA
      atggataat ttagaagca aatatcatg gacaagtca 
      atgcgttcg gtctttgtg ggcaacatc ccctacgaa 
      gccaccgaa gagaagctc aaagacatt ttcggcgaa 
      gtaggccag gtcctgtca ttcaagttg gttttcgac 
      cgcgagaca ggcaagccc aaaggctac ggcttctgc 
      gagtaccgc gaccaggag acggcgctc agcgccatg 
      cgcaacctc aacgggtac gagatcggg gggcgcaac 
      ctccgggtc gacaacgcc tgtaccgag aaatcgcgc 
      atggagatg caaaacttg ctgaaccag cccccggtc 
      gagaacccc tacggcgag ccgattcag gccgagaag 
      gcccccgag gccatcagc aaagccgtg gcctcgttg 
      ccccccgag cagatgttc gagttgatg aagcagatg 
      aagctctgc attcagaac aaccccact gaggccagg 
      cagatgctg ctgcagaac ccccagttg gcgtacgct 
      ctgctgcag gcgcaggtg gttatgagg attattgac 
      ccgcatacg gcggctaca atgttgcat ccgcaaaat 
      cccgttcct tcgtctttg acgcccaag gaaaagact 
      aatccaggc cccattccg attaatagt aattatgtc 
      tcgaatccc acgcctgtg cagccccca agacaggac 
      tttcagccg gtgccagcc cccgcgatt cataataat 
      tccaatccg gtcttcaca ggtcaagac gttgaccta 
      agatcactg gacccccga ggggctgat ccccgcctc 
      agccgcctg ggcgaccaa gacatgcgc actttgccc 
      ggacaactc cccaacccc cttccgccg ccacttgtc 
      gaaaacttt aatagggac ccacgtgga ggtccggcc 
      aacttccca tctgatccg agacaacaa cagcgagct 
      gacccaaga cagaaaaat atgccccct cagcaaaac 
      gcgatgcct ccgcagccg gtcagagcc ccgcagggg 
      ccccccagt accccccaa gttcgccaa gctgccaat 
      gttgcttca agtataggt acagctggg gcctcagac 
      caggaaaag gcggccctg attatgcaa gtcctgcaa 
      ttgtcagat gagcagatt gcaatgctg ccacctgaa 
      cagcgtaat agtattcta gttttgaag gaacaaatc 
      gccaaaagt gcacaccgt tga
    mRNA sequence
    • >TC004378 RA
      tacgattca ctttagggt tgtaaccac aatagataa 
      accaacatt tttgttatt gttggcttt aaaatgcat 
      aattttgta cttttttaa aaggtagag ggttggtaa 
      cgctgaccg tttgttgaa attggtgtc ccaggtgtc 
      ttgattact tcgttccaa gttggaatt aaacaatca 
      ccatggata atttagaag caaatatca tggacaagt 
      caatgcgtt cggtctttg tgggcaaca tcccctacg 
      aagccaccg aagagaagc tcaaagaca ttttcggcg 
      aagtaggcc aggtcctgt cattcaagt tggttttcg 
      accgcgaga caggcaagc ccaaaggct acggcttct 
      gcgagtacc gcgaccagg agacggcgc tcagcgcca 
      tgcgcaacc tcaacgggt acgagatcg gggggcgca 
      acctccggg tcgacaacg cctgtaccg agaaatcgc 
      gcatggaga tgcaaaact tgctgaacc agcccccgg 
      tcgagaacc cctacggcg agccgattc aggccgaga 
      aggcccccg aggccatca gcaaagccg tggcctcgt 
      tgccccccg agcagatgt tcgagttga tgaagcaga 
      tgaagctct gcattcaga acaacccca ctgaggcca 
      ggcagatgc tgctgcaga acccccagt tggcgtacg 
      ctctgctgc aggcgcagg tggttatga ggattattg 
      acccgcata cggcggcta caatgttgc atccgcaaa 
      atcccgttc cttcgtctt tgacgccca aggaaaaga 
      ctaatccag gccccattc cgattaata gtaattatg 
      tctcgaatc ccacgcctg tgcagcccc caagacagg 
      actttcagc cggtgccag cccccgcga ttcataata 
      attccaatc cggtcttca caggtcaag acgttgacc 
      taagatcac tggaccccc gaggggctg atccccgcc 
      tcagccgcc tgggcgacc aagacatgc gcactttgc 
      ccggacaac tccccaacc cccttccgc cgccacttg 
      tcgaaaact ttaataggg acccacgtg gaggtccgg 
      ccaacttcc catctgatc cgagacaac aacagcgag 
      ctgacccaa gacagaaaa atatgcccc ctcagcaaa 
      acgcgatgc ctccgcagc cggtcagag ccccgcagg 
      ggcccccca gtacccccc aagttcgcc aagctgcca 
      atgttgctt caagtatag gtacagctg gggcctcag 
      accaggaaa aggcggccc tgattatgc aagtcctgc 
      aattgtcag atgagcaga ttgcaatgc tgccacctg 
      aacagcgta atagtattc tagttttga aggaacaaa 
      tcgccaaaa gtgcacacc gttgattaa ttaagtcat 
      ttccccaaa tcagtttaa tttttacag tttacatga 
      taa