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Gene information
General information
TC004369 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid77: 125866..140652
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004369
    iBeetle screen iB_00683
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004369 RA
      MLLQTIPGD PGAKSDETS KQPDLDTTG AHVLDYAPA 
      PGWTVHANN EGRLYYCNH VTRTAGWLP PAEAWKCGG 
      GEEILPYGW ERAVDNSGR PYYINHVNK TTTYETPII 
      RNLDPAPIP EPRSVVLER SAALGFGFV AGSEKPVIV 
      RFVTEGGPS VDKLLPGDQ ILTVNGEDV KMAPREHVI 
      SLVRACTKT VNLVVCQPP EQQGIRKST LLSASKKAR 
      LKTKPSRVR FAESVCVNG APLFPPSAF SLGDLCVAP 
      MANVLKVFL ENGQTKSFK YDAWTSVQD VVTSLETKL 
      CLQATEHFS LVVEHIKSL KRNKLTLLD PSDTLARIA 
      SRPGAHKLR CLFRVAFVP PSAAALAQR DLNALDYLY 
      TQCCNDVIQ ERFAPELQY DTALRLAAL HIYQHALAN 
      NIAASKLTV KTIEREFGL DRFVPASLL DTMKRKEVR 
      KLISHFLKL HSNMAGSGK QLTPLQAKL HYLDIVAQL 
      PSYGAKCFS AGPKADALE RVILVSPKF GLSQITGTR 
      NSMPVPIAN IEDMRRIEV RCDDEVTKN VLVRLANEK 
      TVSISLEER DASELVLVL RGYFRLVTG QVLPVDQDE 
      APPMEDLAP PYLSQHKVV PEKWSYINQ SALKTACFA 
      IPPTYQTLN KKTNGLYNT MGRQSKSLG YSNLDNNMN 
      NSLSNRNHF DSYDLETVV SMELLENGR GDDVLKRVA 
      EMHQLVENS EKYLRLNSL EWQETSVDI ESENEDSLP 
      GQLKHSDSL LLLNKSHAP PPIERRFAN AAIELLRSE 
      SNQSESDND SLYTPHDSP KRRIDVKAN RVSFGLRSP 
      DEKNEEAKG EESVYVFDP DIIDLTLLP PPATPDELD 
      CALPTPINV PPSSFADSV EKLNRLDNL NEDQDLEEF 
      LASVTVPPP TQKVTPAIE LTPEEIMSY IIPPPPVEI 
      NTEMTPPPL PPIEPKRRN SNVSIKSNI IEYATIDRK 
      GAFSSCCGK TKKDEQVRP LPRRSSDEK PPERPPKNL 
      PQERQRSQS LTTKPPKLP PRGEAPPHL FLPPKKPPL 
      PPVPPLEVL RQKKNPQID GRTAGIGSP HLQRSRLGS 
      DLDMGLKVA QTAVSTPTS PHLARGAQL TAVPIMSPD 
      SPTQKNGCT STRELLLAK TDVAMASLL VRLDQVAAQ 
      CSAAQVHGG GRLISEEKF QIAKEELTS QSLQLVTSS 
      KMLVIAMSD PSLPDLPEN LAICLTILR RLTELCQDL 
      TNHTTAPLQ TRNLILKVH DVTAAFRHL ITSHIDRTS 
      PKTIEEHLA AQAESLANV LATLLRSLR VFSP
    CDS sequence
    • >TC004369 RA
      atgctactt cagacgatt ccgggcgat cccggagcc 
      aaatcagat gaaacgtcg aaacaaccg gacttggat 
      acgaccgga gcacacgtt ttggattat gctccggcc 
      cccggatgg actgttcat gcaaacaac gagggacgg 
      ctctactat tgcaatcac gtgacacgc accgccggc 
      tggttaccg cccgccgaa gcatggaaa tgtggtggc 
      ggcgaggaa attttgccc tacggttgg gaacgagct 
      gtcgataat tcaggacgt ccttattac atcaatcat 
      gtgaacaaa actacaact tatgaaacc cccattatc 
      agaaatctg gacccagcc cccattcca gagccccgt 
      tctgtggtt ttggaacgt tcggcagca ctcggtttt 
      ggtttcgtt gccggttcg gaaaaaccc gttattgtt 
      cgttttgtt acggaggga ggtcccagt gttgataaa 
      cttctacca ggtgatcaa attcttaca gttaatggt 
      gaagatgta aaaatggca ccacgtgaa catgtcatt 
      tctttggta agagcttgt acaaaaact gtcaacttg 
      gtggtatgt caaccaccc gaacaacaa ggtatacgt 
      aaatcaaca cttctatca gcaagtaaa aaagcacgt 
      ttgaaaacg aaaccatca agagtgcgt tttgccgag 
      agtgtttgc gttaatggc gctccttta tttccgcct 
      tctgcgttt tcattgggt gatttatgt gtggctccc 
      atggccaac gttcttaaa gtatttctc gaaaatgga 
      caaacaaaa tcatttaaa tatgacgct tggacaagt 
      gtacaagat gttgtgacg tcacttgaa actaaacta 
      tgtcttcaa gcaactgaa cattttagt cttgttgtt 
      gaacatatt aaatcactc aaacgtaat aaactcaca 
      ctattggat ccttcggat acactagct cggattgcg 
      tcacgtcca ggggcacac aaattacgt tgtttgttt 
      cgtgtggca tttgttccg ccctcggca gccgccttg 
      gcgcaacgt gatcttaac gccttggat tatctctat 
      acacaatgt tgtaacgat gtgatacag gaacggttt 
      gcgcctgaa ctacaatac gatactgca ctgcgattg 
      gctgcactg catatttat caacacgct ctggccaat 
      aatattgca gcaagcaaa ttgacggtt aagacaatt 
      gaacgcgaa ttcggtctc gatcgtttc gtcccggcg 
      tcacttctc gacacaatg aagcgtaaa gaagtccga 
      aaactgata tcacatttt cttaaactt cattccaat 
      atggccggt tccggtaaa caattgaca ccacttcaa 
      gcaaaactt cattatttg gatattgtt gcacagttg 
      cctagttac ggtgcaaaa tgtttttcg gctggtccg 
      aaagccgac gcactcgaa cgtgtaata cttgtaagt 
      ccgaaattt ggacttagt caaataacg ggaactaga 
      aattcgatg ccggtaccg attgctaat attgaagat 
      atgagacga attgaggtt agatgtgat gatgaagtg 
      acgaaaaat gttttagtt cgtttagcc aatgagaaa 
      actgtttca atctcattg gaagagaga gatgcaagc 
      gagttagtg cttgtattg agaggttat ttccgattg 
      gttactgga caagtgttg ccagtcgat caggacgaa 
      gctccgccc atggaagat ctagcaccg ccttatcta 
      tcacaacat aaagttgtg ccggaaaaa tggagttac 
      atcaaccaa tcagcttta aaaaccgct tgttttgca 
      ataccacca acttatcaa acactaaac aaaaaaacc 
      aacggttta tacaacaca atgggacgt cagagcaaa 
      tcacttggt tacagtaac cttgacaac aacatgaat 
      aattcactc tcgaatcgc aaccatttc gatagttac 
      gaccttgaa acggtagtt tccatggaa ctgctcgaa 
      aatgggcgt ggcgatgac gtacttaaa cgcgtggca 
      gaaatgcac caattggtc gaaaactcc gagaaatat 
      ctccgcctc aattcgctt gaatggcaa gagacgagt 
      gtcgatatc gaatcggaa aacgaagat tcacttccc 
      ggccaatta aaacacagc gattcgctt ctcctactt 
      aataaaagt catgccccg cccccgatt gaacgtcga 
      ttcgctaac gccgccatt gaattgtta cgtagcgaa 
      tcgaaccaa tccgaaagc gataatgat tcgttgtat 
      acaccgcat gattcgcca aaacgtcgt attgatgtg 
      aaagcgaat cgtgttagt tttggttta cgaagtcct 
      gatgagaaa aacgaagag gctaaaggc gaggaaagt 
      gtgtatgtt tttgatccg gatattatt gatttgacg 
      ttgttaccg ccacctgca acgcccgat gaactcgat 
      tgtgcctta ccgacaccg attaacgtg ccgccgagc 
      tcgttcgcc gattccgtc gagaagctc aatcgttta 
      gacaattta aacgaagac caagacctc gaggagttc 
      ctagcgagc gtaacggtc ccacctcca actcaaaaa 
      gttaccccc gcgattgaa ctcacccct gaagagata 
      atgtcatac ataatcccc ccaccacct gttgaaata 
      aatactgaa atgactcca cccccacta ccacccatc 
      gaaccgaaa cgtcgcaat agcaacgtt agcatcaaa 
      tctaacata atcgaatat gcaactatt gatcgtaaa 
      ggtgcattt tcatcatgt tgtggtaaa acgaaaaaa 
      gacgaacaa gtccgacca cttccaaga cgaagttcc 
      gatgaaaaa ccaccagaa cgtccgcct aaaaaccta 
      ccacaagaa cgtcaacgc agccaatca ctaaccacc 
      aaaccaccg aaactccca ccacgtggt gaagccccg 
      ccccattta ttcctccca ccgaaaaaa cctccttta 
      ccaccagtt ccacctcta gaagtacta agacaaaag 
      aaaaatcca caaattgat ggtagaact gcaggaatt 
      ggatcgcca catttgcaa agaagtagg ctggggagt 
      gaccttgat atgggttta aaggtggct cagacggct 
      gtgagtaca ccgacttcg ccgcatttg gctcgaggg 
      gcacaattg acagctgta ccaatcatg tcacctgat 
      agtccaacg caaaaaaat ggttgtacg tcaacaagg 
      gaactccta ttggctaaa actgacgtt gcaatggct 
      agtctttta gttcggttg gatcaagta gccgcacaa 
      tgtagtgct gcacaggtg catggtggg gggagattg 
      atcagcgag gagaagttt cagatcgcc aaagaagag 
      ctcacttca caatcactt caacttgtc acctcgtca 
      aaaatgctc gtgatcgca atgtccgat ccgagttta 
      cccgatcta cctgaaaat ctagccatt tgtttgacg 
      attttacgt cgtctaact gaactgtgt caagatctg 
      accaatcat acaacagct ccacttcaa acacgtaat 
      ctcatcctc aaagtacac gatgtgaca gctgctttc 
      cgccatctt attacgtca cacattgac cgaacatca 
      ccgaaaaca atcgaagaa catctagcg gcacaagcc 
      gaaagtttg gcaaatgtg ttggcaaca ctgttgagg 
      agtttgcga gtgttttca ccgtag
    mRNA sequence
    • >TC004369 RA
      tactttatt ttctattat tattagggg taattgtca 
      agtcgataa aatcgctcc ctgtaattg ctaattgtc 
      tgtagaggg gggctcgaa ttgttttga aatttttca 
      gtcttgcgc gagctttcg tggggggaa tacattcag 
      tctcagttc gtaaaaagt gtaaaaatc agggggtgg 
      tcatagtgt ctgtgttat tatcactag ttggtgttt 
      aaatgggtt tgaagtcgg agtgattgt accatgagt 
      tggcccctt ttgtaccat cccatgacc atgctgggc 
      gtcctggaa gtatagcga tgctacttc agacgattc 
      cgggcgatc ccggagcca aatcagatg aaacgtcga 
      aacaaccgg acttggata cgaccggag cacacgttt 
      tggattatg ctccggccc ccggatgga ctgttcatg 
      caaacaacg agggacggc tctactatt gcaatcacg 
      tgacacgca ccgccggct ggttaccgc ccgccgaag 
      catggaaat gtggtggcg gcgaggaaa ttttgccct 
      acggttggg aacgagctg tcgataatt caggacgtc 
      cttattaca tcaatcatg tgaacaaaa ctacaactt 
      atgaaaccc ccattatca gaaatctgg acccagccc 
      ccattccag agccccgtt ctgtggttt tggaacgtt 
      cggcagcac tcggttttg gtttcgttg ccggttcgg 
      aaaaacccg ttattgttc gttttgtta cggagggag 
      gtcccagtg ttgataaac ttctaccag gtgatcaaa 
      ttcttacag ttaatggtg aagatgtaa aaatggcac 
      cacgtgaac atgtcattt ctttggtaa gagcttgta 
      caaaaactg tcaacttgg tggtatgtc aaccacccg 
      aacaacaag gtatacgta aatcaacac ttctatcag 
      caagtaaaa aagcacgtt tgaaaacga aaccatcaa 
      gagtgcgtt ttgccgaga gtgtttgcg ttaatggcg 
      ctcctttat ttccgcctt ctgcgtttt cattgggtg 
      atttatgtg tggctccca tggccaacg ttcttaaag 
      tatttctcg aaaatggac aaacaaaat catttaaat 
      atgacgctt ggacaagtg tacaagatg ttgtgacgt 
      cacttgaaa ctaaactat gtcttcaag caactgaac 
      attttagtc ttgttgttg aacatatta aatcactca 
      aacgtaata aactcacac tattggatc cttcggata 
      cactagctc ggattgcgt cacgtccag gggcacaca 
      aattacgtt gtttgtttc gtgtggcat ttgttccgc 
      cctcggcag ccgccttgg cgcaacgtg atcttaacg 
      ccttggatt atctctata cacaatgtt gtaacgatg 
      tgatacagg aacggtttg cgcctgaac tacaatacg 
      atactgcac tgcgattgg ctgcactgc atatttatc 
      aacacgctc tggccaata atattgcag caagcaaat 
      tgacggtta agacaattg aacgcgaat tcggtctcg 
      atcgtttcg tcccggcgt cacttctcg acacaatga 
      agcgtaaag aagtccgaa aactgatat cacattttc 
      ttaaacttc attccaata tggccggtt ccggtaaac 
      aattgacac cacttcaag caaaacttc attatttgg 
      atattgttg cacagttgc ctagttacg gtgcaaaat 
      gtttttcgg ctggtccga aagccgacg cactcgaac 
      gtgtaatac ttgtaagtc cgaaatttg gacttagtc 
      aaataacgg gaactagaa attcgatgc cggtaccga 
      ttgctaata ttgaagata tgagacgaa ttgaggtta 
      gatgtgatg atgaagtga cgaaaaatg ttttagttc 
      gtttagcca atgagaaaa ctgtttcaa tctcattgg 
      aagagagag atgcaagcg agttagtgc ttgtattga 
      gaggttatt tccgattgg ttactggac aagtgttgc 
      cagtcgatc aggacgaag ctccgccca tggaagatc 
      tagcaccgc cttatctat cacaacata aagttgtgc 
      cggaaaaat ggagttaca tcaaccaat cagctttaa 
      aaaccgctt gttttgcaa taccaccaa cttatcaaa 
      cactaaaca aaaaaacca acggtttat acaacacaa 
      tgggacgtc agagcaaat cacttggtt acagtaacc 
      ttgacaaca acatgaata attcactct cgaatcgca 
      accatttcg atagttacg accttgaaa cggtagttt 
      ccatggaac tgctcgaaa atgggcgtg gcgatgacg 
      tacttaaac gcgtggcag aaatgcacc aattggtcg 
      aaaactccg agaaatatc tccgcctca attcgcttg 
      aatggcaag agacgagtg tcgatatcg aatcggaaa 
      acgaagatt cacttcccg gccaattaa aacacagcg 
      attcgcttc tcctactta ataaaagtc atgccccgc 
      ccccgattg aacgtcgat tcgctaacg ccgccattg 
      aattgttac gtagcgaat cgaaccaat ccgaaagcg 
      ataatgatt cgttgtata caccgcatg attcgccaa 
      aacgtcgta ttgatgtga aagcgaatc gtgttagtt 
      ttggtttac gaagtcctg atgagaaaa acgaagagg 
      ctaaaggcg aggaaagtg tgtatgttt ttgatccgg 
      atattattg atttgacgt tgttaccgc cacctgcaa 
      cgcccgatg aactcgatt gtgccttac cgacaccga 
      ttaacgtgc cgccgagct cgttcgccg attccgtcg 
      agaagctca atcgtttag acaatttaa acgaagacc 
      aagacctcg aggagttcc tagcgagcg taacggtcc 
      cacctccaa ctcaaaaag ttacccccg cgattgaac 
      tcacccctg aagagataa tgtcataca taatccccc 
      caccacctg ttgaaataa atactgaaa tgactccac 
      ccccactac cacccatcg aaccgaaac gtcgcaata 
      gcaacgtta gcatcaaat ctaacataa tcgaatatg 
      caactattg atcgtaaag gtgcatttt catcatgtt 
      gtggtaaaa cgaaaaaag acgaacaag tccgaccac 
      ttccaagac gaagttccg atgaaaaac caccagaac 
      gtccgccta aaaacctac cacaagaac gtcaacgca 
      gccaatcac taaccacca aaccaccga aactcccac 
      cacgtggtg aagccccgc cccatttat tcctcccac 
      cgaaaaaac ctcctttac caccagttc cacctctag 
      aagtactaa gacaaaaga aaaatccac aaattgatg 
      gtagaactg caggaattg gatcgccac atttgcaaa 
      gaagtaggc tggggagtg accttgata tgggtttaa 
      aggtggctc agacggctg tgagtacac cgacttcgc 
      cgcatttgg ctcgagggg cacaattga cagctgtac 
      caatcatgt cacctgata gtccaacgc aaaaaaatg 
      gttgtacgt caacaaggg aactcctat tggctaaaa 
      ctgacgttg caatggcta gtcttttag ttcggttgg 
      atcaagtag ccgcacaat gtagtgctg cacaggtgc 
      atggtgggg ggagattga tcagcgagg agaagtttc 
      agatcgcca aagaagagc tcacttcac aatcacttc 
      aacttgtca cctcgtcaa aaatgctcg tgatcgcaa 
      tgtccgatc cgagtttac ccgatctac ctgaaaatc 
      tagccattt gtttgacga ttttacgtc gtctaactg 
      aactgtgtc aagatctga ccaatcata caacagctc 
      cacttcaaa cacgtaatc tcatcctca aagtacacg 
      atgtgacag ctgctttcc gccatctta ttacgtcac 
      acattgacc gaacatcac cgaaaacaa tcgaagaac 
      atctagcgg cacaagccg aaagtttgg caaatgtgt 
      tggcaacac tgttgagga gtttgcgag tgttttcac 
      cgtagagaa aaaaatcta gtcagacta atttgacac 
      ttgatgacc aaataaatg aacaatcgc gcaataatt 
      acgtgactg aatttgtga taattatca aaaagttaa 
      aacaagtat gatctgtcc gatgcgatt tgctttata 
      atttttttt tattattat tatttaagt ttgctagtc 
      acgagcagt acaatcgat ggttttttg ttaccctgt 
      gaatgttga aagaaaatg tatatttga ttgttaatt 
      tagttgaga aaaacaagt atttaagtt tgttgtaaa 
      ttttataaa tgtaaaact gtttaatta tttattact 
      aaggtgtat gtaacgtct ctaacatat cctgtatgt 
      tgaaataat aaaaacatg cgataaaaa attacatga 
      aagtgtaaa aaaacaaaa aatcagtcc caaataaag 
      aactattaa atta