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Gene information
General information
TC004358 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid77: 233692..234622
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004358
    iBeetle screen iB_00681
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004358 RA
      MAMFGLIVS GRPVQTEFQ PISETQFIT 
      TIPEADSIN HIVVFLTGA IPFPEGTAG 
      QVYFSWPDP HAPPNWQPL GHISNIKPS 
      AIFKISSLK KLEEMGDSM NILFGQSHI 
      VHNAQIGIA IEPLANIRE IESANNPSN 
      NVTFAQKML ENFMNFVLS YTVTQPQMV 
      PDPTATYVP LATVQNWYT NFERRLQQN 
      PNFWKT
    CDS sequence
    • >TC004358 RA
      atggcaatg ttcggtttg atagtatca gggcgtccg 
      gtccaaacc gaattccag ccgatttcc gagacacaa 
      ttcataacc acaatccca gaagccgac agtataaac 
      cacatcgtg gtgttccta acaggggct ataccgttc 
      cccgaaggc accgccggt caagtctac ttcagttgg 
      cctgaccca cacgcgccc cccaactgg cagcccctc 
      ggccacatt tccaacata aaaccgtcg gcaattttc 
      aaaatctca tcactgaaa aaactcgaa gagatgggc 
      gactcgatg aatatttta ttcgggcaa agtcacatc 
      gtacacaat gcgcaaata gggatcgct attgagccg 
      ctggcgaat attcgggaa atcgaatcg gcgaataat 
      cccagtaat aatgtcacg tttgctcag aaaatgttg 
      gagaatttt atgaatttt gtgctgagt tatacggtg 
      acgcaaccg caaatggtc ccagatccc acggccacg 
      tacgttccg ttggctaca gtacagaac tggtacacg 
      aattttgaa cgacggttg caacaaaac cccaatttt 
      tggaaaacg tag
    mRNA sequence
    • >TC004358 RA
      atgccagtc cttgttctt gtccgaagt gaaacccat 
      caagctttt ttgatgaaa tttcgctat aatttgcta 
      aatcaaaca gaaacgtta ttaaatttg gtgacttat 
      tttttaggg attttgtgg ttgtggcgt ccctaacct 
      aacaaattt gagaaatgt tctagaagc ttcgtgatg 
      gcaatgttc ggtttgata gtatcaggg cgtccggtc 
      caaaccgaa ttccagccg atttccgag acacaattc 
      ataaccaca atcccagaa gccgacagt ataaaccac 
      atcgtggtg ttcctaaca ggggctata ccgttcccc 
      gaaggcacc gccggtcaa gtctacttc agttggcct 
      gacccacac gcgcccccc aactggcag cccctcggc 
      cacatttcc aacataaaa ccgtcggca attttcaaa 
      atctcatca ctgaaaaaa ctcgaagag atgggcgac 
      tcgatgaat attttattc gggcaaagt cacatcgta 
      cacaatgcg caaataggg atcgctatt gagccgctg 
      gcgaatatt cgggaaatc gaatcggcg aataatccc 
      agtaataat gtcacgttt gctcagaaa atgttggag 
      aattttatg aattttgtg ctgagttat acggtgacg 
      caaccgcaa atggtccca gatcccacg gccacgtac 
      gttccgttg gctacagta cagaactgg tacacgaat 
      tttgaacga cggttgcaa caaaacccc aatttttgg 
      aaaacgtag aggaatgtg tgcatacgt gtgtcttgt 
      gttcatcaa agctggatt atggcggct gttaagtct 
      caaaactca ctcgctttt aatgtattt aaatgaata 
      aagtatcga ttttttt