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Gene information
General information
TC004356 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid77: 269699..273522
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004356
    iBeetle screen iB_00680
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 leg musculature femural musculature partially not fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    Comment: muscles in the femur seem not to converge
    leg musculature tb1-ptar1 size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    legs tibiotarsus sometimes shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 4


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004356 RA
      MATALYTDK DTNDELWKE CSKWLTRWG MLRADHRAN 
      WPDACIADL ANILRDGVL LCKLLNKIS PGCIDMKNV 
      NLKPAMAQF LSLHNIELF LKTCVISFG LKDQDLFDP 
      LVLFELTNF HKVLCTLSK LSLTKEAQK SNILGFTAQ 
      GVKTKEEDV IYQSLKSVE MPTNARGMN DPIYDVAGE 
      EIYQDLCAL RPVNEQPQT FEKRDFVIK ELLDTEKNY 
      VEVLAKLKR NFMKPLANQ MKPQDHEVV FYKIYDLHE 
      IHLAFYREL TRFRDPGFR LSSIFMNWR ERFLVYGGY 
      CANLTRATN LLQELCDCD ENFNATIVA YEKEDNNGR 
      FKLRDVLSV PMQRILKYH LLLEKLIEN TDPNDEECN 
      DLRRAREAM LDVAGFINE AARDSEHLA VIQNLQENI 
      VEWYRSPDH RLVNYGRLI KDGEMKIKA HDDQKIRNR 
      YVFIFDKCI LICKQIKVR QFAFRNIIN ISEYHVDDT 
      HNRAVLSRD ARFCYHFHL VKNDNVMVY TIFVRTLEL 
      KQQMIKAIN DALDNIHPA AIKRTNHTF ELQTFNGPV 
      QCFHCSKYL RGLIYQGYK CGVCGIGVH KHCIAHSGR 
      CGSHNHHST SDLVMNGAD ACLRDKLWF VGEMDRSRA 
      QNELERREN GTFLVRIRP QSEDKDKYA LSLKTNDTV 
      KHMKICSTG EHEGKYYLS LSKFFSNIE ELVLNYQTN 
      SLKENFERL EENTKLLWP YRQLLATVI RNFEPTEIC 
      HLPLREGQI IYVIGKEGY REGWWKGRN DRQESGFFP 
      SNAVRIDGE ARLNTSRHF TQ
    • >TC004356 RB
      MATALYTDK DTNDELWKE CSKWLTRWG MLRADHRAN 
      WPDACIADL ANILRDGVL LCKLLNKIS PGCIDMKNV 
      NLKPAMAQF LSLHNIELF LKTCVISFG LKDQDLFDP 
      LVLFELTNF HKVLCTLSK LSLTKEAQK SNILGFTAQ 
      GVKTKEEDV IYQSLKSVY VRTNEPSWL QFTIPCPRP 
      EDREEEVYD DLCYVTFST CLPEVCIIF PPVLYLSPV 
      YYFVACLFC FCYIREMPT NARGMNDPI YDVAGEEIY 
      QDLCALRPV NEQPQTFEK RDFVIKELL DTEKNYVEV 
      LAKLKRNFM KPLANQMKP QDHEVVFYK IYDLHEIHL 
      AFYRELTRF RDPGFRLSS IFMNWRERF LVYGGYCAN 
      LTRATNLLQ ELCDCDENF NATIVAYEK EDNNGRFKL 
      RDVLSVPMQ RILKYHLLL EKLIENTDP NDEECNDLR 
      RAREAMLDV AGFINEAAR DSEHLAVIQ NLQENIVEW 
      YRSPDHRLV NYGRLIKDG EMKIKAHDD QKIRNRYVF 
      IFDKCILIC KQIKVRQFA FRNIINISE YHVDDTHNR 
      AVLSRDARF CYHFHLVKN DNVMVYTIF VRTLELKQQ 
      MIKAINDAL DNIHPAAIK RTNHTFELQ TFNGPVQCF 
      HCSKYLRGL IYQGYKCGV CGIGVHKHC IAHSGRCGS 
      HNHHSTSDL VMNGADACL RDKLWFVGE MDRSRAQNE 
      LERRENGTF LVRIRPQSE DKDKYALSL KTNDTVKHM 
      KICSTGEHE GKYYLSLSK FFSNIEELV LNYQTNSLK 
      ENFERLEEN TKLLWPYRQ LLATVIRNF EPTEICHLP 
      LREGQIIYV IGKEGYREG WWKGRNDRQ ESGFFPSNA 
      VRIDGEARL NTSRHFTQ
    CDS sequence
    • >TC004356 RA
      atggccaca gctctttat accgataaa gacaccaac 
      gacgaactg tggaaagag tgttcgaaa tggttaaca 
      cgatggggg atgttaaga gcagaccat cgagccaat 
      tggcctgat gcttgtatt gccgatttg gctaacatc 
      ctccgagat ggtgtctta ctttgtaaa ttgctcaat 
      aagataagt cctggatgt attgatatg aagaatgtt 
      aatttgaaa ccggccatg gctcaattt ttaagtttg 
      cacaacatt gagctgttc ctcaagact tgtgttatc 
      tcgtttgga ttgaaggat caggatttg tttgatcca 
      ctggtgctg ttcgagctc acaaatttc cataaagta 
      ttatgcact ttatcaaaa ctttcacta actaaagaa 
      gcccaaaaa agcaacatt ttaggattt actgctcaa 
      ggtgtcaaa acgaaagaa gaagatgtg atttatcaa 
      agtctcaaa tctgtagaa atgccaacc aatgctaga 
      ggaatgaat gatcccatt tatgatgta gcaggcgaa 
      gaaatttat caggatttg tgtgcactg aggccagtt 
      aatgagcag ccccaaacg ttcgaaaaa cgtgatttt 
      gttataaaa gaactgtta gatacggaa aaaaattat 
      gttgaagtt cttgccaaa ctgaaacgg aattttatg 
      aagccgtta gctaatcaa atgaaaccc caagatcat 
      gaagttgtt ttttataaa atttacgat ttacacgaa 
      atacattta gctttttat cgggaattg acccgattt 
      cgcgatccc ggctttaga ttaagttcg atttttatg 
      aactggagg gaaagattt cttgtttat ggtggttat 
      tgtgcgaat ttaacacgt gcaacgaat ctgttgcaa 
      gaattgtgt gattgtgat gagaatttt aacgcaacg 
      attgtggcg tacgaaaaa gaagacaat aatggtagg 
      tttaaactt agagatgtt ttatcggtt ccgatgcaa 
      agaatctta aaatatcat ttattactt gaaaaattg 
      atcgaaaat actgatcct aacgacgaa gaatgtaat 
      gatttgcga cgagctcgt gaagcgatg cttgatgtt 
      gccggtttt ataaacgaa gcggcacga gatagtgag 
      catttggca gtaattcaa aatttgcaa gaaaatatt 
      gtggaatgg tacagatca ccggatcat cgtttagta 
      aattatgga agattgatt aaagatggt gaaatgaaa 
      atcaaagca catgatgat caaaaaatc aggaaccga 
      tacgtgttc atctttgac aaatgtatc ttgatttgc 
      aaacaaatc aaagttcgt caatttgca tttcgaaat 
      atcatcaat atcagcgaa taccacgtg gatgataca 
      cataataga gctgtattg agtcgagat gcgcgtttt 
      tgttatcat tttcatctt gttaagaac gacaatgtt 
      atggtttat acgattttt gtccgaact cttgaattg 
      aaacaacaa atgattaaa gcgataaat gatgctttg 
      gataatatc catccagct gctatcaaa cgtacaaat 
      cacacattc gaattgcaa acttttaac ggtccagtt 
      caatgtttt cattgctca aaatattta cgaggtttg 
      atttatcaa ggttataaa tgtggagtt tgcggaatc 
      ggtgtccat aagcattgt atagcacat tcgggtcgt 
      tgtggctca cataatcat cattcaacg tccgattta 
      gtcatgaat ggagccgat gcttgtctt agagataaa 
      ttatggttt gttggtgaa atggaccga agtcgcgca 
      caaaatgaa ttagaacga cgagaaaat ggcacgttt 
      ctagtcagg attagacca cagagtgaa gataaggac 
      aagtacgca ctatcgctc aaaacaaat gataccgtc 
      aaacacatg aaaatatgc agcactgga gaacacgaa 
      ggaaaatat tacttatct ctatcgaag ttttttagc 
      aacattgag gaattggtg ctcaattat caaacaaat 
      agtttaaag gagaatttc gagagactg gaagaaaat 
      acgaaattg ttgtggcct taccgtcaa ttgctcgca 
      accgttatt cgaaatttt gaaccgacc gagatttgt 
      catttgccg cttcgggaa ggtcaaata atctatgtt 
      ataggtaaa gaagggtat agggaggga tggtggaag 
      ggccgaaac gaccgacag gaaagtggt ttttttcct 
      agtaatgct gtccgaatt gatggtgaa gcgagattg 
      aacactagt cgtcatttt acgcaataa
    • >TC004356 RB
      atggccaca gctctttat accgataaa gacaccaac 
      gacgaactg tggaaagag tgttcgaaa tggttaaca 
      cgatggggg atgttaaga gcagaccat cgagccaat 
      tggcctgat gcttgtatt gccgatttg gctaacatc 
      ctccgagat ggtgtctta ctttgtaaa ttgctcaat 
      aagataagt cctggatgt attgatatg aagaatgtt 
      aatttgaaa ccggccatg gctcaattt ttaagtttg 
      cacaacatt gagctgttc ctcaagact tgtgttatc 
      tcgtttgga ttgaaggat caggatttg tttgatcca 
      ctggtgctg ttcgagctc acaaatttc cataaagta 
      ttatgcact ttatcaaaa ctttcacta actaaagaa 
      gcccaaaaa agcaacatt ttaggattt actgctcaa 
      ggtgtcaaa acgaaagaa gaagatgtg atttatcaa 
      agtctcaaa tctgtctat gtcaggacc aacgaacca 
      agttggtta caatttaca attccttgt ccgcgacct 
      gaagacaga gaagaggag gtttacgac gatttgtgt 
      tacgtgacc ttctcgact tgtctgcca gaggtttgc 
      ataattttc cccccagtg ttgtatcta tcgcctgtt 
      tattatttt gttgcatgc ctgttttgt ttttgttac 
      attagagaa atgccaacc aatgctaga ggaatgaat 
      gatcccatt tatgatgta gcaggcgaa gaaatttat 
      caggatttg tgtgcactg aggccagtt aatgagcag 
      ccccaaacg ttcgaaaaa cgtgatttt gttataaaa 
      gaactgtta gatacggaa aaaaattat gttgaagtt 
      cttgccaaa ctgaaacgg aattttatg aagccgtta 
      gctaatcaa atgaaaccc caagatcat gaagttgtt 
      ttttataaa atttacgat ttacacgaa atacattta 
      gctttttat cgggaattg acccgattt cgcgatccc 
      ggctttaga ttaagttcg atttttatg aactggagg 
      gaaagattt cttgtttat ggtggttat tgtgcgaat 
      ttaacacgt gcaacgaat ctgttgcaa gaattgtgt 
      gattgtgat gagaatttt aacgcaacg attgtggcg 
      tacgaaaaa gaagacaat aatggtagg tttaaactt 
      agagatgtt ttatcggtt ccgatgcaa agaatctta 
      aaatatcat ttattactt gaaaaattg atcgaaaat 
      actgatcct aacgacgaa gaatgtaat gatttgcga 
      cgagctcgt gaagcgatg cttgatgtt gccggtttt 
      ataaacgaa gcggcacga gatagtgag catttggca 
      gtaattcaa aatttgcaa gaaaatatt gtggaatgg 
      tacagatca ccggatcat cgtttagta aattatgga 
      agattgatt aaagatggt gaaatgaaa atcaaagca 
      catgatgat caaaaaatc aggaaccga tacgtgttc 
      atctttgac aaatgtatc ttgatttgc aaacaaatc 
      aaagttcgt caatttgca tttcgaaat atcatcaat 
      atcagcgaa taccacgtg gatgataca cataataga 
      gctgtattg agtcgagat gcgcgtttt tgttatcat 
      tttcatctt gttaagaac gacaatgtt atggtttat 
      acgattttt gtccgaact cttgaattg aaacaacaa 
      atgattaaa gcgataaat gatgctttg gataatatc 
      catccagct gctatcaaa cgtacaaat cacacattc 
      gaattgcaa acttttaac ggtccagtt caatgtttt 
      cattgctca aaatattta cgaggtttg atttatcaa 
      ggttataaa tgtggagtt tgcggaatc ggtgtccat 
      aagcattgt atagcacat tcgggtcgt tgtggctca 
      cataatcat cattcaacg tccgattta gtcatgaat 
      ggagccgat gcttgtctt agagataaa ttatggttt 
      gttggtgaa atggaccga agtcgcgca caaaatgaa 
      ttagaacga cgagaaaat ggcacgttt ctagtcagg 
      attagacca cagagtgaa gataaggac aagtacgca 
      ctatcgctc aaaacaaat gataccgtc aaacacatg 
      aaaatatgc agcactgga gaacacgaa ggaaaatat 
      tacttatct ctatcgaag ttttttagc aacattgag 
      gaattggtg ctcaattat caaacaaat agtttaaag 
      gagaatttc gagagactg gaagaaaat acgaaattg 
      ttgtggcct taccgtcaa ttgctcgca accgttatt 
      cgaaatttt gaaccgacc gagatttgt catttgccg 
      cttcgggaa ggtcaaata atctatgtt ataggtaaa 
      gaagggtat agggaggga tggtggaag ggccgaaac 
      gaccgacag gaaagtggt ttttttcct agtaatgct 
      gtccgaatt gatggtgaa gcgagattg aacactagt 
      cgtcatttt acgcaataa
    mRNA sequence
    • >TC004356 RA
      taatttcgc aaatcggca gcctccaaa ttccacaaa 
      cgacaaaaa taacaccac aaaagtgta aaaaatcgt 
      atttttttt taatttttt cgtggaaat aggtggcgc 
      tcccatcgt tcgtcatag caacgcgaa aattgacat 
      taataataa tgtcacttt gacgttaaa gtaaattta 
      ttttattta ctgattgta tttatcggt ttattttgt 
      tggaaaagt gtgcgagat tagtgcttt tcgatattt 
      caatgttcc aggagctta caatatggc cacagctct 
      ttataccga taaagacac caacgacga actgtggaa 
      agagtgttc gaaatggtt aacacgatg ggggatgtt 
      aagagcaga ccatcgagc caattggcc tgatgcttg 
      tattgccga tttggctaa catcctccg agatggtgt 
      cttactttg taaattgct caataagat aagtcctgg 
      atgtattga tatgaagaa tgttaattt gaaaccggc 
      catggctca atttttaag tttgcacaa cattgagct 
      gttcctcaa gacttgtgt tatctcgtt tggattgaa 
      ggatcagga tttgtttga tccactggt gctgttcga 
      gctcacaaa tttccataa agtattatg cactttatc 
      aaaactttc actaactaa agaagccca aaaaagcaa 
      cattttagg atttactgc tcaaggtgt caaaacgaa 
      agaagaaga tgtgattta tcaaagtct caaatctgt 
      agaaatgcc aaccaatgc tagaggaat gaatgatcc 
      catttatga tgtagcagg cgaagaaat ttatcagga 
      tttgtgtgc actgaggcc agttaatga gcagcccca 
      aacgttcga aaaacgtga ttttgttat aaaagaact 
      gttagatac ggaaaaaaa ttatgttga agttcttgc 
      caaactgaa acggaattt tatgaagcc gttagctaa 
      tcaaatgaa accccaaga tcatgaagt tgtttttta 
      taaaattta cgatttaca cgaaataca tttagcttt 
      ttatcggga attgacccg atttcgcga tcccggctt 
      tagattaag ttcgatttt tatgaactg gagggaaag 
      atttcttgt ttatggtgg ttattgtgc gaatttaac 
      acgtgcaac gaatctgtt gcaagaatt gtgtgattg 
      tgatgagaa ttttaacgc aacgattgt ggcgtacga 
      aaaagaaga caataatgg taggtttaa acttagaga 
      tgttttatc ggttccgat gcaaagaat cttaaaata 
      tcatttatt acttgaaaa attgatcga aaatactga 
      tcctaacga cgaagaatg taatgattt gcgacgagc 
      tcgtgaagc gatgcttga tgttgccgg ttttataaa 
      cgaagcggc acgagatag tgagcattt ggcagtaat 
      tcaaaattt gcaagaaaa tattgtgga atggtacag 
      atcaccgga tcatcgttt agtaaatta tggaagatt 
      gattaaaga tggtgaaat gaaaatcaa agcacatga 
      tgatcaaaa aatcaggaa ccgatacgt gttcatctt 
      tgacaaatg tatcttgat ttgcaaaca aatcaaagt 
      tcgtcaatt tgcatttcg aaatatcat caatatcag 
      cgaatacca cgtggatga tacacataa tagagctgt 
      attgagtcg agatgcgcg tttttgtta tcattttca 
      tcttgttaa gaacgacaa tgttatggt ttatacgat 
      ttttgtccg aactcttga attgaaaca acaaatgat 
      taaagcgat aaatgatgc tttggataa tatccatcc 
      agctgctat caaacgtac aaatcacac attcgaatt 
      gcaaacttt taacggtcc agttcaatg ttttcattg 
      ctcaaaata tttacgagg tttgattta tcaaggtta 
      taaatgtgg agtttgcgg aatcggtgt ccataagca 
      ttgtatagc acattcggg tcgttgtgg ctcacataa 
      tcatcattc aacgtccga tttagtcat gaatggagc 
      cgatgcttg tcttagaga taaattatg gtttgttgg 
      tgaaatgga ccgaagtcg cgcacaaaa tgaattaga 
      acgacgaga aaatggcac gtttctagt caggattag 
      accacagag tgaagataa ggacaagta cgcactatc 
      gctcaaaac aaatgatac cgtcaaaca catgaaaat 
      atgcagcac tggagaaca cgaaggaaa atattactt 
      atctctatc gaagttttt tagcaacat tgaggaatt 
      ggtgctcaa ttatcaaac aaatagttt aaaggagaa 
      tttcgagag actggaaga aaatacgaa attgttgtg 
      gccttaccg tcaattgct cgcaaccgt tattcgaaa 
      ttttgaacc gaccgagat ttgtcattt gccgcttcg 
      ggaaggtca aataatcta tgttatagg taaagaagg 
      gtataggga gggatggtg gaagggccg aaacgaccg 
      acaggaaag tggtttttt tcctagtaa tgctgtccg 
      aattgatgg tgaagcgag attgaacac tagtcgtca 
      ttttacgca ataaatagt cgcacgact tgccagtat 
      aattgtttt gaagtgttc acactgcag gagaaagaa 
      agaaagcat ttattaatg tgtaattaa taaatttta 
      tattataga tgtgttaag tgtatcggt gccattttt 
      ttttttttt ttgaaatcc gataatttg ggacggcct 
      ttgaaatac aacgaaagc caccccaaa tgaataatt 
      tttgttaaa tgaaatata catactata tattttatg 
      ctagataca cggataaat tttgttatt cttaaatgt 
      atgtttaat aaatcaaag tattgttt
    • >TC004356 RB
      taatttcgc aaatcggca gcctccaaa ttccacaaa 
      cgacaaaaa taacaccac aaaagtgta aaaaatcgt 
      atttttttt taatttttt cgtggaaat aggtggcgc 
      tcccatcgt tcgtcatag caacgcgaa aattgacat 
      taataataa tgtcacttt gacgttaaa gtaaattta 
      ttttattta ctgattgta tttatcggt ttattttgt 
      tggaaaagt gtgcgagat tagtgcttt tcgatattt 
      caatgttcc aggagctta caatatggc cacagctct 
      ttataccga taaagacac caacgacga actgtggaa 
      agagtgttc gaaatggtt aacacgatg ggggatgtt 
      aagagcaga ccatcgagc caattggcc tgatgcttg 
      tattgccga tttggctaa catcctccg agatggtgt 
      cttactttg taaattgct caataagat aagtcctgg 
      atgtattga tatgaagaa tgttaattt gaaaccggc 
      catggctca atttttaag tttgcacaa cattgagct 
      gttcctcaa gacttgtgt tatctcgtt tggattgaa 
      ggatcagga tttgtttga tccactggt gctgttcga 
      gctcacaaa tttccataa agtattatg cactttatc 
      aaaactttc actaactaa agaagccca aaaaagcaa 
      cattttagg atttactgc tcaaggtgt caaaacgaa 
      agaagaaga tgtgattta tcaaagtct caaatctgt 
      ctatgtcag gaccaacga accaagttg gttacaatt 
      tacaattcc ttgtccgcg acctgaaga cagagaaga 
      ggaggttta cgacgattt gtgttacgt gaccttctc 
      gacttgtct gccagaggt ttgcataat tttcccccc 
      agtgttgta tctatcgcc tgtttatta ttttgttgc 
      atgcctgtt ttgtttttg ttacattag agaaatgcc 
      aaccaatgc tagaggaat gaatgatcc catttatga 
      tgtagcagg cgaagaaat ttatcagga tttgtgtgc 
      actgaggcc agttaatga gcagcccca aacgttcga 
      aaaacgtga ttttgttat aaaagaact gttagatac 
      ggaaaaaaa ttatgttga agttcttgc caaactgaa 
      acggaattt tatgaagcc gttagctaa tcaaatgaa 
      accccaaga tcatgaagt tgtttttta taaaattta 
      cgatttaca cgaaataca tttagcttt ttatcggga 
      attgacccg atttcgcga tcccggctt tagattaag 
      ttcgatttt tatgaactg gagggaaag atttcttgt 
      ttatggtgg ttattgtgc gaatttaac acgtgcaac 
      gaatctgtt gcaagaatt gtgtgattg tgatgagaa 
      ttttaacgc aacgattgt ggcgtacga aaaagaaga 
      caataatgg taggtttaa acttagaga tgttttatc 
      ggttccgat gcaaagaat cttaaaata tcatttatt 
      acttgaaaa attgatcga aaatactga tcctaacga 
      cgaagaatg taatgattt gcgacgagc tcgtgaagc 
      gatgcttga tgttgccgg ttttataaa cgaagcggc 
      acgagatag tgagcattt ggcagtaat tcaaaattt 
      gcaagaaaa tattgtgga atggtacag atcaccgga 
      tcatcgttt agtaaatta tggaagatt gattaaaga 
      tggtgaaat gaaaatcaa agcacatga tgatcaaaa 
      aatcaggaa ccgatacgt gttcatctt tgacaaatg 
      tatcttgat ttgcaaaca aatcaaagt tcgtcaatt 
      tgcatttcg aaatatcat caatatcag cgaatacca 
      cgtggatga tacacataa tagagctgt attgagtcg 
      agatgcgcg tttttgtta tcattttca tcttgttaa 
      gaacgacaa tgttatggt ttatacgat ttttgtccg 
      aactcttga attgaaaca acaaatgat taaagcgat 
      aaatgatgc tttggataa tatccatcc agctgctat 
      caaacgtac aaatcacac attcgaatt gcaaacttt 
      taacggtcc agttcaatg ttttcattg ctcaaaata 
      tttacgagg tttgattta tcaaggtta taaatgtgg 
      agtttgcgg aatcggtgt ccataagca ttgtatagc 
      acattcggg tcgttgtgg ctcacataa tcatcattc 
      aacgtccga tttagtcat gaatggagc cgatgcttg 
      tcttagaga taaattatg gtttgttgg tgaaatgga 
      ccgaagtcg cgcacaaaa tgaattaga acgacgaga 
      aaatggcac gtttctagt caggattag accacagag 
      tgaagataa ggacaagta cgcactatc gctcaaaac 
      aaatgatac cgtcaaaca catgaaaat atgcagcac 
      tggagaaca cgaaggaaa atattactt atctctatc 
      gaagttttt tagcaacat tgaggaatt ggtgctcaa 
      ttatcaaac aaatagttt aaaggagaa tttcgagag 
      actggaaga aaatacgaa attgttgtg gccttaccg 
      tcaattgct cgcaaccgt tattcgaaa ttttgaacc 
      gaccgagat ttgtcattt gccgcttcg ggaaggtca 
      aataatcta tgttatagg taaagaagg gtataggga 
      gggatggtg gaagggccg aaacgaccg acaggaaag 
      tggtttttt tcctagtaa tgctgtccg aattgatgg 
      tgaagcgag attgaacac tagtcgtca ttttacgca 
      ataaatagt cgcacgact tgccagtat aattgtttt 
      gaagtgttc acactgcag gagaaagaa agaaagcat 
      ttattaatg tgtaattaa taaatttta tattataga 
      tgtgttaag tgtatcggt gccattttt ttttttttt 
      ttgaaatcc gataatttg ggacggcct ttgaaatac 
      aacgaaagc caccccaaa tgaataatt tttgttaaa 
      tgaaatata catactata tattttatg ctagataca 
      cggataaat tttgttatt cttaaatgt atgtttaat 
      aaatcaaag tattgttt