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Gene information
General information
TC004273 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3897520..3899354
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004273
    iBeetle screen iB_00675

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004273 RA
      MKLFLISLC FAFAQSAKI LGVFNHAGA SHTSIGKVL 
      LKNLAKRGH HVTMISSYP MDEPVDNYR DIFLYENLE 
      DFKQREKMY FTKDKTNEL ETLRNMTLT HTENTFTNP 
      QVKELLESN ETFDLVIID WFFNEASLI FGHRLKAPV 
      IYVSAFGNM LFLNDFTGN TLPYSYVPG AALSTSDEM 
      SFKHRVVMT LLNLGYNLV LPWRNQAQY EILKRHFVD 
      PPSIEELKE NIALVLAVS HFSFETSRP YTPSVVPVG 
      GFHIDELKQ LPKDLEKFL NSAKNGAIF FSLGSQIKS 
      TNLEKNTFA SIVKALGKL PQKILWKYE SDDFINLPK 
      NIKIVKWAP QLEILAHPN VKLFISHCG TLSFIESIH 
      FNKPLLCLP FNGDQLTNA AFARSRQFG AHISPDEIT 
      EDSLFNKTT EVLTNPLYR KQMNFHSSL LREQPMKPM 
      DLAIFWVEH VINHRTGDH LKTFATKLP WYKYYLVDV 
      IGFFLGVVF IVVKIVLFL IKFVVRRVS RLSKSEIKA 
      KND
    CDS sequence
    • >TC004273 RA
      atgaagtta tttttaatt tcgctctgt ttcgcattt 
      gcccaaagc gcaaaaatt ctgggggtg ttcaaccat 
      gcgggggcc agtcacaca tccattggt aaagtgcta 
      ttgaaaaat ttggccaag agggggcac cacgttacc 
      atgatcagt tcctaccca atggacgaa ccagtggat 
      aactacagg gatattttc ctttatgaa aatttggaa 
      gatttcaaa caacgtgaa aaaatgtac ttcactaaa 
      gacaaaacc aacgaattg gagacgcta aggaacatg 
      acgttaact cacaccgaa aacactttt accaacccc 
      caagtgaaa gaacttctc gagtcaaat gagactttt 
      gatttggtg ataattgat tggtttttc aacgaagct 
      tcgcttatt ttcggccac cggctcaaa gcgccggtg 
      atttacgtg tcggcgttt ggaaatatg ctgtttctg 
      aacgacttt acgggaaat actttaccg tattcttac 
      gtcccgggt gctgcattg tccactagt gacgaaatg 
      tcctttaaa catcgggtg gtaatgact ttactgaac 
      ttgggctat aatttagtg cttccctgg cggaatcaa 
      gcccagtat gagattctc aagcgccat ttcgttgat 
      cctccaagt attgaagaa cttaaagaa aatatagcg 
      ctggttttg gccgtttct cacttcagt tttgagact 
      tcaagacct tacacaccc agtgttgta ccagtgggt 
      ggtttccac atcgacgaa ttgaaacaa ttacccaaa 
      gacttggaa aaatttctc aatagtgcc aaaaacggg 
      gcgattttt ttcagtctc gggagtcaa ataaagtcg 
      actaatctt gaaaaaaac acttttgca agcatagtc 
      aaagctttg ggtaaacta ccacagaaa atattgtgg 
      aagtacgaa agcgacgac tttattaat ttgcccaaa 
      aacataaaa atcgtcaag tgggcaccg cagttggaa 
      attttggcg caccctaat gttaaattg tttatttct 
      cactgtggc actttaagt ttcattgaa tcgattcat 
      ttcaataaa cccttgctt tgtcttccc tttaacggg 
      gaccagctc acgaacgca gcctttgct cggtcgaga 
      cagtttggg gctcacatt tctccagat gaaatcaca 
      gaagacagt ctgtttaat aaaaccaca gaagtgtta 
      actaaccca ttatatcgg aagcaaatg aattttcac 
      tcgtctttg ctgcgagag cagcccatg aaaccaatg 
      gatttggcc attttttgg gtggaacat gtgataaat 
      caccgaact ggtgatcat ttaaaaact ttcgctacg 
      aagcttccg tggtataaa tattatctt gtcgatgtt 
      attgggttt tttctgggt gtggttttt attgttgtt 
      aaaattgtg ttgttttta attaagttt gttgttcga 
      agggtgagt aggttgtct aagagtgaa attaaggcg 
      aaaaatgat taa
    mRNA sequence
    • >TC004273 RA
      ttgtttaat ttaaaacca gtcaccatg aagttattt 
      ttaatttcg ctctgtttc gcatttgcc caaagcgca 
      aaaattctg ggggtgttc aaccatgcg ggggccagt 
      cacacatcc attggtaaa gtgctattg aaaaatttg 
      gccaagagg gggcaccac gttaccatg atcagttcc 
      tacccaatg gacgaacca gtggataac tacagggat 
      attttcctt tatgaaaat ttggaagat ttcaaacaa 
      cgtgaaaaa atgtacttc actaaagac aaaaccaac 
      gaattggag acgctaagg aacatgacg ttaactcac 
      accgaaaac acttttacc aacccccaa gtgaaagaa 
      cttctcgag tcaaatgag acttttgat ttggtgata 
      attgattgg tttttcaac gaagcttcg cttattttc 
      ggccaccgg ctcaaagcg ccggtgatt tacgtgtcg 
      gcgtttgga aatatgctg tttctgaac gactttacg 
      ggaaatact ttaccgtat tcttacgtc ccgggtgct 
      gcattgtcc actagtgac gaaatgtcc tttaaacat 
      cgggtggta atgacttta ctgaacttg ggctataat 
      ttagtgctt ccctggcgg aatcaagcc cagtatgag 
      attctcaag cgccatttc gttgatcct ccaagtatt 
      gaagaactt aaagaaaat atagcgctg gttttggcc 
      gtttctcac ttcagtttt gagacttca agaccttac 
      acacccagt gttgtacca gtgggtggt ttccacatc 
      gacgaattg aaacaatta cccaaagac ttggaaaaa 
      tttctcaat agtgccaaa aacggggcg atttttttc 
      agtctcggg agtcaaata aagtcgact aatcttgaa 
      aaaaacact tttgcaagc atagtcaaa gctttgggt 
      aaactacca cagaaaata ttgtggaag tacgaaagc 
      gacgacttt attaatttg cccaaaaac ataaaaatc 
      gtcaagtgg gcaccgcag ttggaaatt ttggcgcac 
      cctaatgtt aaattgttt atttctcac tgtggcact 
      ttaagtttc attgaatcg attcatttc aataaaccc 
      ttgctttgt cttcccttt aacggggac cagctcacg 
      aacgcagcc tttgctcgg tcgagacag tttggggct 
      cacatttct ccagatgaa atcacagaa gacagtctg 
      tttaataaa accacagaa gtgttaact aacccatta 
      tatcggaag caaatgaat tttcactcg tctttgctg 
      cgagagcag cccatgaaa ccaatggat ttggccatt 
      ttttgggtg gaacatgtg ataaatcac cgaactggt 
      gatcattta aaaactttc gctacgaag cttccgtgg 
      tataaatat tatcttgtc gatgttatt gggtttttt 
      ctgggtgtg gtttttatt gttgttaaa attgtgttg 
      tttttaatt aagtttgtt gttcgaagg gtgagtagg 
      ttgtctaag agtgaaatt aaggcgaaa aatgattaa 
      aatttgtag aatgttttt tgaatttat tgttggatt 
      ttttgtctt ggaagttat tattttatt attctttga 
      aataaaaat ttattgcag tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY